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The Crop Journal 2024年第2期12卷 456-469页

作者： Jingli ding Chenchen Ji Lu Yu Chuang Wang guangda ding Sheliang Wang Lei Shi Fangsen Xu Hongmei Cai Research Center of Microelement Huazhong Agricultural UniversityWuhan 430070HubeiChina College of Resources and Environment Huazhong Agricultural UniversityWuhan 430070HubeiChina National Key Laboratory of Crop Genetics and Improvement Huazhong Agricultural UniversityWuhan 430070HubeiChina

Transcription factors regulating crop uptake and translocation of the micronutrient Cu have not been *** isolated a novel R2R3-MYB transcription factor,OsMYB84,and showed that it was a positive regulator involved in uptake and transport of Cu via activation of OsCOPT2 and OsHMA ***84 was highly expressed in roots and anthers and induced by *** of OsMYB84 promoted uptake and root-to-shoot translocation of Cu in rice,facilitated Cu distribution into grain and increased grain *** contrast,mutation of OsMYB84 reduced Cu concentration in xylem ***84 bound to the promoter region of OsCOPT2 and OsHMA5 and upregulated their ***2 mutants showed reduced uptake of Cu and OsHMA5 overexpression lines showed increased root-to-shoot translocation of Cu.

关键词： OsMYB84 OsCOPT2 OsHMA5 Copper Rice

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遮阴栽培对雪茄茄衣烟叶叶面均匀性的影响

中国农业科技导报 2024年第2期26卷 216-225页

作者：郭昊谭荣雷杨锦鹏余君黄文昌杨久红乔保明杨瑞玮徐芳森杨春雷丁广大华中农业大学资源与环境学院微量元素研究中心武汉430070 湖北省烟草科学研究院武汉430030 中国烟草总公司湖北省公司烟叶管理处武汉430030

为探究遮阴栽培对雪茄茄衣品种叶面均匀性的影响,以雪茄茄衣品种‘楚雪26号’(CX-26)为研究对象,在大田试验条件下进行不同程度的遮阴处理,分析其成熟期上部叶和中部叶的叶面厚度、叶面平展度、叶片密度、主支脉粗细、主支脉占叶片干物... 详细信息

为探究遮阴栽培对雪茄茄衣品种叶面均匀性的影响,以雪茄茄衣品种‘楚雪26号’(CX-26)为研究对象,在大田试验条件下进行不同程度的遮阴处理,分析其成熟期上部叶和中部叶的叶面厚度、叶面平展度、叶片密度、主支脉粗细、主支脉占叶片干物质量比例以及SPAD值等的差异。结果表明,随着遮阴程度的增加,上部叶的叶面厚度、叶片密度以及叶片SPAD值均逐渐下降;当透光率为70%时,叶面厚度、叶片密度与不遮阴处理相比分别下降35.5%和33.3%,而中部叶中各性状则逐渐上升。遮阴栽培显著增加上部叶的叶面平展度,但降低了中部叶的叶面平展度;在透光率为70%时,上部叶的叶面平展度达到最大,为0.98;遮阴栽培增加上部叶主支脉占叶片干物质量比例,对中部叶主支脉占叶片干物质量比例的影响随遮阴程度的增加呈下降趋势,但差异不显著。遮阴栽培对中部叶主支脉的粗细程度无影响,但使上部叶的主支脉变细。以上研究结果为国产雪茄茄衣烟叶遮阴栽培技术提供理论基础。

关键词：雪茄烟遮阴栽培透光率叶面均匀性

Induction of jasmonic acid biosynthetic genes inhibits Arabidopsis growth in response to low boron

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Journal of Integrative Plant Biology 2021年第5期63卷 937-948页

作者： Yupu Huang Sheliang Wang Chuang Wang guangda ding Hongmei Cai Lei Shi Fangsen Xu National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070China Microelement Research Center College of Resources&EnvironmentHuazhong Agricultural UniversityWuhan 430070China

The essential micronutrient boron(B) has key roles in cell wall integrity and B deficiency inhibits plant growth. The role of jasmonic acid(JA) in plant growth inhibition under B deficiency remains unclear. Here,we report that low B elevates JA biosynthesis in Arabidopsis thaliana by inducing the expression of JA biosynthesis genes. Treatment with JA inhibited plant growth and, a JA biosynthesis inhibitor enhanced plant growth, indicating that the JA induced by B deficiency affects plant growth. Furthermore,examination of the JA signaling mutants jasmonate resistant1, coronatine insensitive1-2, and myc2 showed that JA signaling negatively regulates plant growth under B deficiency. We identified a low-B responsive transcription factor, ERF018, and used yeast one-hybrid assays and transient activation assays in Nicotiana benthamiana leaf cells to demonstrate that ERF018 activates the expression of JA biosynthesis genes. ERF018 overexpression(OE)lines displayed stunted growth and up-regulation of JA biosynthesis genes under normal B conditions,compared to Col-0 and the difference between ERF018 OE lines and Col-0 diminished under low *** results suggest that ERF018 enhances JA biosynthesis and thus negatively regulates plant growth. Taken together, our results highlight the importance of JA in the effect of low B on plant growth.

关键词： Arabidopsis thaliana ERF018 low B jasmonic acid biosynthesis jasmonic acid signaling plant growth

Vascular Cambium-Localized AtSPDT Mediates Xylem-to-Phloem Transfer of Phosphorus for Its Preferential Distribution in Arabidopsis

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Molecular Plant 2020年第1期13卷 99-111页

作者： guangda ding Gui Jie Lei Naoki Yamaji Kengo Yokosho Namiki Mitani-Ueno Sheng Huang Jian Feng Ma institute of Plant Science and Resources Okayama UniversityChuo 2-20-1KurashikiJapan College of Resources and Environment Huazhong Agricultural UniversityWuhan 430070China

During plant growth and development mineral elements are preferentially delivered to different organs and tissues to meet the differential demand. It has been shown that the preferential distribution of mineral nutrients in gramineous plants is mediated by node-based transporters, but the mechanisms of preferential distribution in dicots are poorly understood. Here, we report a distinct mechanism for the preferential distribution of phosphorus (P) in Arabidopsis plants, revealed by detailed functional analysis of AtSPDT/AtSULTR3;4 (SULTR-like P Distribution Transporter), a homolog of rice OsSPDT. Like OsSPDT, AtSPDT is localized at the plasma membrane and showed proton-dependent transport activity for P. Interestingly, we found that AtSPDT is mainly expressed in the rosette basal region and leaf petiole, and its expression is up-regulated by P deficiency. Tissue-specific analysis showed that AtSPDT is mainly located in the vascular cambium of different organs, as well as in the parenchyma tissues of both xylem and phloem regions. Knockout of AtSPDT inhibited the growth of new leaves under low P due to decreased P distribution to those organs. The seed yields of the wild-type and atspdt mutant plants are similar, but the seeds of mutant plants contain – less P. These results indicate that AtSPDT localized in the vascular cambium is involved in preferential distribution of P to the developing tissues, through xylem-to-phloem transfer mainly at the rosette basal region and leaf petiole.

关键词： A rabidopsis thaliana phosphate transporter SPDT phosphorus distribution vascular cambium

A further analysis of BnSPX3;2 promoter in Brassica napus indicated an unknown cis-acting element responsive to phosphorus stress

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A further analysis of BnSPX3;2 promoter in Brassica napus in...

甘蓝型油菜耐铝极端品种筛选及耐铝生理机制初步解析

第十四届全国植物基因组学大会

作者： Hongyuan Du Guangzhe Yang guangda ding Lei Shi Fangsen Xu National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University

Phosphorus(P) is an essential macronutrient for *** of the low availability of phosphate(Pi) absorbed directly by plant roots in soil,plants have evolved many adaptive *** the proteins carrying the SPX(Syg1/Pho81/Xpr1... 详细信息

关键词： Brassica napus SPX domain phosphate promoter cis-acting element

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华中农业大学学报 2023年第6期42卷 154-163页

作者：张雯黄益丹张鹏超徐芳森王创丁广大华中农业大学资源与环境学院/微量元素研究中心/农业农村部长江中下游耕地保育重点实验室武汉430070

为筛选不同铝敏感性油菜品种并探究油菜对铝毒的耐受机制,以997份甘蓝型油菜自然品种为材料,采用土培初筛和营养液复筛的两步筛选法,以地上部鲜质量和相对根系伸长率为指标筛选获得甘蓝型油菜不同铝敏感性品种,解析油菜耐铝的生理机制... 详细信息

为筛选不同铝敏感性油菜品种并探究油菜对铝毒的耐受机制,以997份甘蓝型油菜自然品种为材料,采用土培初筛和营养液复筛的两步筛选法,以地上部鲜质量和相对根系伸长率为指标筛选获得甘蓝型油菜不同铝敏感性品种,解析油菜耐铝的生理机制。结果显示,997份甘蓝型油菜自然品种苗期在酸性土壤上表现出显著的基因型差异,从中筛选出142个不同铝敏感性极端品种,含77份铝耐受品种和65份铝敏感品种;进一步利用营养液培养体系在加铝和不加铝处理下对142个油菜品种进行复筛,通过聚类分析发现,97个品种在2个培养体系中具有较好的生长一致性;经过多次表型重复试验最终确定2个铝耐受品种(806和985)和2个铝敏感品种(482和811)。在铝毒处理下铝敏感品种(482和811)生物量相比不加铝处理下降约45%,根系生长显著被抑制,而铝耐受品种则无明显差异。进一步分析发现,相比铝敏感品种,铝耐受品种在铝毒条件下根系中积累的铝更少,活性氧含量更低,且根系中抗氧化酶(CAT和POD)的活性相比无铝处理显著增加,这可能是油菜耐铝的重要生理机制之一。

关键词：甘蓝型油菜酸性土壤铝毒两步筛选法极端品种耐铝

甘蓝型油菜苗期耐酸性综合评价及耐酸种质筛选

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华中农业大学学报 2023年第6期42卷 164-174页

作者：张鹏超张雯黄益丹冉文昊徐芳森丁广大华中农业大学资源与环境学院/微量元素研究中心/农业农村部长江中下游耕地保育重点实验室武汉430070

为探究在酸性土壤环境下油菜苗期耐酸特性,采用盆栽试验对113份油菜品种进行酸胁迫处理和酸性土壤改良处理,在苗期测定根长、株高、地上部鲜质量、根系鲜质量、总鲜质量、地上部干质量、根系干质量、总干质量、根冠比和SPAD共10项性状指... 详细信息

为探究在酸性土壤环境下油菜苗期耐酸特性,采用盆栽试验对113份油菜品种进行酸胁迫处理和酸性土壤改良处理,在苗期测定根长、株高、地上部鲜质量、根系鲜质量、总鲜质量、地上部干质量、根系干质量、总干质量、根冠比和SPAD共10项性状指标,利用相关性分析、主成分分析、隶属函数法、聚类分析、灰色关联度和逐步回归分析对不同油菜品种进行耐酸综合评价,建立油菜耐酸评价模型并筛选耐酸油菜品种。结果显示:113份油菜品种在2个处理下10个性状均呈现极显著差异;10项指标的变异系数范围为10.09%~78.68%,将10个单项指标转换成4个相互独立的综合指标,代表了原始指标88.688%的信息量;基于113份油菜的耐酸综合评价值(D值)聚类分析,将油菜品种分为5个类群,分别为极耐酸型(2份)、耐酸型(16份)、中间型(41份)、敏感型(50份)、极敏感型(4份);通过D值和10项指标构建最优回归方程:D=-0.075+0.0403X_(总干质量)+0.100X_(根系鲜质量)+0.188XSPAD+0.099X_(株高)+0.092X_(根系干质量)(R^(2)=0.982,F=1154.562^(***)),筛选出适宜的油菜苗期耐酸鉴定指标。综上,酸胁迫会严重抑制油菜苗期的生长,不同油菜品种耐酸性存在较大差异,通过测定总干质量、根系鲜质量、SPAD、株高、根系干质量并结合回归方程可评价判断油菜耐酸性。

关键词：甘蓝型油菜苗期酸性土壤耐酸性综合评价耐酸品种

A population for genome-wide association studies(GWAS) of root traits in oilseed rape(Brassic napus L.)

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A population for genome-wide association studies(GWAS) of ro...

QTLs for seed yield and yield-related traits under low phosphorus level in Brassica napus

第十四届全国植物基因组学大会

作者： Xiaohua Wang Yanling Chen guangda ding Fangsen Xu Tingdong Fu Lei Shi Bin Yi National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University

Genome-wide associate studies(GWAS) have been employed to reveal the genetic bases of some important complex agronomic traits in crops,such as kernel,plant height,flowering time and root number in the germination stag... 详细信息

关键词： genome-wide association studies(GWAS) oilseed rape root traits Single Nucleotide Polymorphism(SNP) phosphorus

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QTLs for seed yield and yield-related traits under low phosp...

基于代谢组学分析雪茄烟叶晾制时“青斑”组织的代谢差异及形成原因

第十三届全国植物基因组学大会

作者： guangda ding,Lei Shi,Fangsen Xu~* National Key Laboratory of Crop Genetic Improvement,Wuhan 430070,China

One of the key targets of breeding programs in rapeseed(Brassica napus) is to develop high-yield ***,the lack of available phosphorus(P) in soils seriously limits rapeseed *** the present study,two-year field trials were conducted at one site with normal and low P treatments using a population of 124 recombinant inbred lines(RILs) derived from a cross between B104-2 and Eyou

关键词：

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华中农业大学学报 2022年第1期41卷 152-159页

作者：于连营杨锦鹏余君李楠芬杨荣洲汪社亮丁广大杨春雷徐芳森华中农业大学作物遗传改良国家重点实验室/微量元素研究中心武汉430070 湖北省烟草科学研究院武汉430030

为探究雪茄烟叶自然晾制过程中青色斑点(简称“青斑”)形成的生理机制及代谢差异,提高茄衣烟叶外观品质,改进晾制技术,以雪茄烟‘楚雪10号’品种为试验材料,利用高压液相色谱串联质谱(HPLC-MS/MS)非靶向代谢组学技术,分析烟叶“青斑”... 详细信息

为探究雪茄烟叶自然晾制过程中青色斑点(简称“青斑”)形成的生理机制及代谢差异,提高茄衣烟叶外观品质,改进晾制技术,以雪茄烟‘楚雪10号’品种为试验材料,利用高压液相色谱串联质谱(HPLC-MS/MS)非靶向代谢组学技术,分析烟叶“青斑”和正常两组织部位的代谢差异。研究结果表明:“青斑”部位叶绿素和绿原酸含量显著高于正常部位,大量未分解的叶绿素和绿原酸在晾制末期沉积是“青斑”形成的生理基础;“青斑”和正常两组织部位间,酪氨酸代谢、苯丙氨酸代谢和类黄酮生物合成途径具有显著差异;绿原酸和二氢玉米素可作为“青斑”形成过程的化学标记物,晾制过程中可检测其含量高低来监测是否会出现“青斑”;烟叶变黄期晾房湿度骤降且波动过大,可能是形成“青斑”的外部环境因素,建议采用湿度可控晾房晾制并适当提高变黄期湿度。

关键词：雪茄烟茄衣 “青斑” 叶绿素非靶向代谢组学烟叶