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10B-doped MCP detector developed for neutron resonance imaging at Back-n white neutron source

Nuclear Science and Techniques 2024年第8期35卷 58-68页

作者： Qiang Li Li‑Jiao Wang Jing‑Yu Tang Xiang‑Biao Qiu Zhen Chen Mao‑yuan Zhao Chang‑Jun Ning Kai Pan Wei Xu Tao Li Su‑Peng Lu Han Yi Rui‑Rui Fan Chang‑Qing Feng Rong Zhang Xiao‑Yang Sun Qi An Hao‑Fan Bai Jiang‑Bo Bai Jie Bao Ping Cao Qi‑Ping Chen Yong‑Hao Chen Zeng‑Qi Cui An‑Chuan Fan Fan‑Zhen Feng Min‑Hao Gu Chang‑Cai Han Zi‑Jie Han Guo‑Zhu He Yong‑Cheng He Yang Hong Yi‑Wei Hu Han‑Xiong Huang Wei Jiang Zhi‑Jie Jiang Zheng‑Yao Jin Ling Kang Bo Li Gong Li Xiao Li Yang Li Jie Liu Rong Liu Shu‑Bin Liu Yi‑Na Liu guang‑yuan luan Jie Ren Zhi‑Zhou Ren Xi‑Chao Ruan Zhao‑Hui Song Kang Sun Zhi‑Xin Tan Sheng‑Da Tang Jin‑Cheng Wang Peng‑Cheng Wang Zhao‑Hui Wang Zhong‑Wei Wen Xiao‑guang Wu Xuan Wu Cong Xia Yong‑Ji Yu Guo‑Hui Zhang Hang‑Chang Zhang Lin‑Hao Zhang Qi‑Wei Zhang Xian‑Peng Zhang Yu‑Liang Zhang Yue Zhang Zhi‑Yong Zhang Zhi‑Hao Zhou Ke‑Jun Zhu Chong Zou Institute of High Energy Physics Chinese Academy of Sciences(CAS)Beijing 100049China Spallation Neutron Source Science Center Dongguan 523803China State Key Laboratory of Particle Detection and Electronics Institute of High Energy Physics Chinese Academy of SciencesBeijing 100049China Dongguan Songshan Lake Future School Dongguan 523808China School of Nuclear Science and Technology University of Science and Technology of ChinaHefei 230027China State Key Laboratory of Particle Detection and Electronics University of Science and Technology of ChinaHefei 230026China North Night Vision Science and Technology(Nanjing)Research Institute Co.Ltd Nanjing 211106China Department of Modern Physics University of Science and Technology of ChinaHefei 230026China State Key Laboratory of Nuclear Physics and Technology School of PhysicsPeking UniversityBeijing 100871China Institute of Modern Physics Fudan UniversityShanghai 200433China Key Laboratory of Nuclear Data China Institute of Atomic EnergyBeijing 102413China China Academy of Engineering Physics Institute of Nuclear Physics and ChemistryMianyang 621900China USTC archaeometry lab University of Science and Technology of ChinaHefei 230026China Northwest Institute of Nuclear Technology Xi’an 710024China University of Chinese Academy of Sciences Beijing 100049China China Shipbuilding Trading Co. LtdBeijing 100044China

Neutron resonance imaging(NRI)has recently emerged as an appealing technique for neutron *** complexity surpasses that of conventional transmission imaging,as it requires a high demand for both a neutron source and ***,the progression of NRI technology has been sluggish since its inception in the 1980s,particularly considering the limited studies analyzing the neutron energy range above *** white neutron source(Back-n)at the China Spallation Neutron Source(CSNS)provides favorable beam conditions for the development of the NRI technique over a wide neutron energy range from eV to ***-sensitive microchannel plates(MCP)have emerged as a cutting-edge tool in the field of neutron detection owing to their high temporal and spatial resolutions,high detection efficiency,and low *** this study,we report the development of a 10B-doped MCP detector,along with its associated electronics,data processing system,and NRI experiments at the *** heavy elements such as gold,silver,tungsten,and indium can be easily identified in the transmission images by their characteristic resonance peaks in the 1–100 eV energy range;the more difficult medium-weight elements such as iron,copper,and aluminum with resonance peaks in the 1–100 keV energy range can also be *** particular,results in the neutron energy range of dozens of keV(Aluminum)are reported here for the first time.

关键词： Neutron resonance imaging 10B doped MCP detector White neutron source Sample nuclide identification

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Nondestructive technique for identifying nuclides using neutron resonance transmission analysis at CSNS Back-n

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Nuclear Science and Techniques 2024年第1期35卷 178-187页

作者： Sheng-Da Tang Yong-Hao Chen Jing-Yu Tang Rui-Rui Fan Qiang Li Gong Li Dong Liu Zheng-Yao Jin Xing-Zhu Cui Tian-Xiang Chen Yi-Wei Yang Rong Liu Han Yi Yang Li Zhen Yang Qi An Hao-Fan Bai Jiang-Bo Bai Jie Bao Ping Cao Qi-Ping Chen Zhen Chen Zeng-Qi Cui An-Chuan Fan Chang-Qing Feng Fan-Zhen Feng Ke-Qing Gao Min-Hao Gu Chang-Cai Han Zi-Jie Han Guo-Zhu He Yong-Cheng He Yang Hong Yi-Wei Hu Han-Xiong Huang Wei-Hua Jia Hao-yu Jiang Wei Jiang Zhi-jie Jiang Ling Kang Bo Li Chao Li Jia-Wen Li Xiao Li Jie Liu Shu-Bin Liu guang-yuan luan Chang-Jun Ning Bin-Bin Qi Jie Ren Zhi-Zhou Ren Xi-Chao Ruan Zhao-Hui Song Kang Sun Zhi-Xin Tan Li-Jiao Wang Peng-Cheng Wang Zhao-Hui Wang Zhong-Wei Wen Xiao-guang Wu Xuan Wu Li-Kun Xie Yong-Ji Yu Guo-Hui Zhang Lin-Hao Zhang Mo-Han Zhang Qi-Wei Zhang Xian-Peng Zhang Yu-Liang Zhang Yue Zhang Zhi-Yong Zhang Mao-yuan Zhao Lu-Ping Zhou Zhi-Hao Zhou Ke-Jun Zhu Sino-French Institute of Nuclear Engineering and Technology Sun Yat-sen UniversityZhuhai 519082China Institute of High-Energy Physics Chinese Academy of Sciences(CAS)Beijing 100049China Spallation Neutron Source Science Center Dongguan 523803China School of Nuclear Science and Technology University of Science and Technology of ChinaHefei 230026China State Key Laboratory of Particle Detection and Electronics University of Science and Technology of ChinaHefei 230026China State Key Laboratory of Particle Detection and Electronics Institute of High Energy PhysicsChinese Academy of SciencesBeijing 100049China USTC Archaeometry Laboratory University of Science and Technology of ChinaHefei 230026China Institute of Nuclear Physics and Chemistry China Academy of Engineering PhysicsMianyang 621900China Department of Modern Physics University of Science and Technology of ChinaHefei 230026China State Key Laboratory of Nuclear Physics and Technology School of PhysicsPeking UniversityBeijing 100871China Key Laboratory of Nuclear Data China Institute of Atomic EnergyBeijing 102413China Northwest Institute of Nuclear Technology Xi’an 710024China University of Chinese Academy of Sciences Beijing 100049China

Nondestructive and noninvasive neutron assays are essential applications of neutron *** resonance transmission analysis(NRTA)is a powerful nondestructive method for investigating the elemental composition of an *** back-streaming neutron line(Back-n)is a newly built time-of-flight facility at the China Spallation Neutron Source(CSNS)that provides neutrons in the eV to 300 MeV range.A feasibility study of the NRTA method for nuclide identification was conducted at the CSNS Back-n via two test *** results demonstrate that it is feasible to identify different elements and isotopes in samples using the NRTA method at *** study reveals its potential future applications.

关键词： CSNS White neutron beam NRTA Nuclide identification Nondestructive method

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Study of the response of 10B-doped MCP to wide-energy range neutrons from eV to MeV

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Nuclear Science and Techniques 2024年第8期35卷 132-141页

作者： Qiang Li Li‑Jiao Wang Xiang‑Biao Qiu Jing‑Wen Li Wei Xu Tao Li Ze‑Bin Lin Chang‑Jun Ning Yong‑Hao Chen Rui‑Rui Fan Kang Sun Jing‑Yu Tang Rong Zhang Han‑Tao Jing Bo Mei Qi An Hao‑Fan Bai Jiang‑Bo Bai Jie Bao Ping Cao Qi‑Ping Chen Zhen Chen Zeng‑Qi Cui An‑Chuan Fan Chang‑Qing Feng Fan‑Zhen Feng Ke‑Qing Gao Min‑Hao Gu Chang‑Cai Han Zi‑Jie Han Guo‑Zhu He Yong‑Cheng He Yang Hong Yi‑Wei Hu Han‑Xiong Huang Wei‑Hua Jia Hao‑Yu Jiang Wei Jiang Zhi‑Jie Jiang Zheng‑Yao Jin Ling Kang Bo Li Chao Li Gong Li Jia‑Wen Li Xiao Li Yang Li Jie Liu Rong Liu Shu‑Bin Liu guang‑yuan luan Bin‑Bin Qi Jie Ren Zhi‑Zhou Ren Xi‑Chao Ruan Zhao‑Hui Song Zhi‑Xin Tan Sheng‑Da Tang Peng‑Cheng Wang Zhao‑Hui Wang Zhong‑Wei Wen Xiao‑guang Wu Xuan Wu Li‑Kun Xie Yi‑Wei Yang Han Yi Yong‑Ji Yu Guo‑Hui Zhang Lin‑Hao Zhang Mo‑Han Zhang Qi‑Wei Zhang Xian‑Peng Zhang Yu‑Liang Zhang Yue Zhang Zhi‑Yong Zhang Mao‑yuan Zhao Lu‑Ping Zhou Zhi‑Hao Zhou Ke‑Jun Zhu Institute of High Energy Physics Chinese Academy of Sciences(CAS)Beijing 100049China Spallation Neutron Source Science Center Dongguan 523803China State Key Laboratory of Particle Detection and Electronics Institute of High Energy PhysicsChinese Academy of SciencesBeijing 100049China Dongguan Songshan Lake Future School Dongguan 523808China North Night Vision Science&Technology(Nanjing)Research Institute Co.Ltd. Nanjing 211106China Sino‑French Institute of Nuclear Engineering and Technology Sun Yat-sen UniversityZhuhai 519082China School of Nuclear Science and Technology University of Science and Technology of ChinaHefei 230027China State Key Laboratory of Particle Detection and Electronics University of Science and Technology of ChinaHefei 230026China Department of Modern Physics University of Science and Technology of ChinaHefei 230026China State Key Laboratory of Nuclear Physics and Technology School of PhysicsPeking UniversityBeijing 100871China Institute of Nuclear Physics and Chemistry China Academy of Engineering PhysicsMianyang 621900China Key Laboratory of Nuclear Data China Institute of Atomic EnergyBeijing 102413China USTC Archaeometry Lab University of Science and Technology of ChinaHefei 230026China Northwest Institute of Nuclear Technology Xi’an 710024China University of Chinese Academy of Sciences Beijing 100049China

Neutron-sensitive microchannel plates(nMCPs)have applications in neutron detection,including energy spectrum measurements,neutron-induced cross sections,and neutron imaging.10B-doped MCPs(B-MCPs)have attracted significant attention owing to their potential for exhibiting a high neutron detection efficiency over a large neutron energy *** spatial and temporal resolutions are useful for neutron energy-resolved ***,their practical applications still face many technical *** this study,a B-MCP with 10 mol%10B was tested for its response to wide-energy neutrons from eV to MeV at the Back-n white neutron source at the China Spallation Neutron *** neutron detection efficiency was calibrated at 1 eV,which is approximately 300 times that of an ordinary MCP and indicates the success of 10 B *** factors that caused the reduction in the detection efficiency were simulated and *** neutron energy spectrum obtained using B-MCP was compared with that obtained by other measurement methods,and showed very good consistency for neutron energies below tens of *** response is more complicated at higher neutron energy,at which point the elastic and nonelastic reactions of all nuclides of B-MCP gradually become *** is beneficial for the detection of neutrons,as it compensates for the detection efficiency of B-MCP for high-energy neutrons.

关键词： Neutron radiation image 10B-doped MCP Neutron response Wide-energy range neutrons

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Ghost Module Based Residual Mixture of Self-Attention and Convolution for Online Signature Verification

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Computers, Materials & Continua 2024年第4期79卷 695-712页

作者： Fangjun luan Xuewen Mu Shuai yuan Department of Computer Technology School of Computer Science and EngineeringShenyang Jianzhu UniversityShenyang110168China Liaoning Province Big Data Management and Analysis Laboratory of Urban Construction Shenyang Jianzhu UniversityShenyang110168China Shenyang Branch of National Special Computer Engineering Technology Research Center Shenyang Jianzhu UniversityShenyang110168China

Online Signature Verification (OSV), as a personal identification technology, is widely used in various ***, it faces challenges, such as incomplete feature extraction, low accuracy, and computational heaviness. Toaddress these issues, we propose a novel approach for online signature verification, using a one-dimensionalGhost-ACmix Residual Network (1D-ACGRNet), which is a Ghost-ACmix Residual Network that combines convolutionwith a self-attention mechanism and performs improvement by using Ghost method. The Ghost-ACmix Residualstructure is introduced to leverage both self-attention and convolution mechanisms for capturing global featureinformation and extracting local information, effectively complementing whole and local signature features andmitigating the problem of insufficient feature extraction. Then, the Ghost-based Convolution and Self-Attention(ACG) block is proposed to simplify the common parts between convolution and self-attention using the Ghostmodule and employ feature transformation to obtain intermediate features, thus reducing computational ***, feature selection is performed using the random forestmethod, and the data is dimensionally reducedusing Principal Component Analysis (PCA). Finally, tests are implemented on the MCYT-100 datasets and theSVC-2004 Task2 datasets, and the equal error rates (EERs) for small-sample training using five genuine andforged signatures are 3.07% and 4.17%, respectively. The EERs for training with ten genuine and forged signaturesare 0.91% and 2.12% on the respective datasets. The experimental results illustrate that the proposed approacheffectively enhances the accuracy of online signature verification.

关键词： Online signature verification feature selection ACG block ghost-ACmix residual structure

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Effects of rare earth elements on inclusions, microstructure and impact toughness of spring steel

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Journal of Iron and Steel Research International 2024年第4期31卷 933-944页

作者： Chun-lei Hao Chao-yun Yang Peng Liu Yi-kun luan Bao-guang Sang Shenyang National Laboratory for Materials Science Institute of Metal ResearchChinese Academy of SciencesShenyang 110016LiaoningChina School of Mechanical Engineering and Automation Dalian Polytechnic UniversityDalian 116034LiaoningChina

Spring steel is an indispensable basic part manufacturing material in mechanical equipment,which is widely used in transportation tools such as automobiles,railways,construction machinery and other *** effects of rare earth(RE)on inclusions,microstructure,carbides and impact toughness of 50CrV spring steel were studied by means of optical microscope,scanning electron microscope,transmission electron microscope and impact *** results show that the addition of rare earth can modify the main MnS and Al_(2)O_(3) inclusions in steel to form spherical or near-spherical rare earth inclusions,and the size of inclusions is significantly *** the quenched structure,the size of martensite slab becomes smaller and the structure is *** tempering,the carbide changes from sheet to ellipse,which makes it easier for spring steel to form stress concentration cracks under *** becomes finer and more dispersed,which leads to better pinning of grain boundaries and smaller grain *** impact toughness of 50CrV-RE spring steel increased from 21 to 41 J by 95%due to the improvement of inclusions,microstructure and carbides by rare *** is possible to improve the strength and toughness of spring steel for the improvement of railway running speed and the development of vehicle lightweight in China.

关键词： Rare earth-Spring steel Inclusion Microstructure Impact toughness

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High-Temperature Stability of Mg-1Al-12Y Alloy Containing LPSO Phase and Mechanism of Its Portevin-Le Chatelier(PLC)Effect

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Acta Metallurgica Sinica(English Letters) 2024年第6期37卷 982-998页

作者： Qian-Long Ren Shuai yuan Shi-Yu luan Jin-Hui Wang Xiao-Wei Li Xiao-Yu Liu Qinghai Provincial Key Laboratory of New Light Alloys Qinghai Provincial Engineering Research Center of High Performance Light Metal Alloys and FormingQinghai UniversityXining810016China School of Materials Science and Engineering Shenyang University of TechnologyShenyang110870China

In this study,the high-temperature stability and the generation mechanism of the Portevin-Le Chatelier(PLC)effect in solid-solution Mg-1Al-12Y alloy with different heat treatment processes were investigated by adjusting the content of long-period stacking ordered(LPSO)*** was found that the content of LPSO phases in the alloys differed the most after heat treatment at 530℃for 16 h and 24 h,with values of 13.56%and 3.93%***,high-temperature tensile experiments were conducted on these two alloys at temperatures of 150℃,200℃,250℃,and 300℃.The results showed that both alloys exhibited the PLC effect at temperatures ranging from 150 to 250℃.However,at a temperature 300℃,only the alloy with a greater concentration of LPSO phases exhibited the PLC effect,whereas the alloy with a lower proportion of LPSO phases did not exhibit this ***,both alloys exhibited remarkable high-temperature stability,with the alloy containing a greater percentage of LPSO phases also demonstrating superior *** underlying mechanism for this phenomenon lies in the exceptional high-temperature stability exhibited by the second phase within the ***,the LPSO phase effectively obstructs the movement of dislocations,and it also undergoing kinking to facilitate plastic deformation of the *** results indicate that the PLC effect can be suppressed by reducing dislocation pile-up at grain boundaries,which leads to a decrease in alloy plasticity but an increase in *** presence of the PLC effect in the WA121 alloy is attributed to the abundant dispersed second phase within the alloy,which initially hinders the movement of dislocations,leading to an increase in stress,and subsequently releases the dislocations,allowing them to continue their movement and thereby reducing in stress.

关键词： Magnesium alloy Long-period stacking ordered(LPSO)phase Portevin-Le Chatelier effect High temperature

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Effects of long-term partial substitution of inorganic fertilizer with pig manure and/or straw on nitrogen fractions and microbiological properties in greenhouse vegetable soils

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Journal of Integrative Agriculture 2024年第6期23卷 2083-2098页

作者： Shuo yuan Ruonan Li Yinjie Zhang Hao'an luan Jiwei Tang Liying Wang Hongjie Ji Shaowen Huang State Key Laboratory of Efficient Utilization of Arid and Semi-arid Arable Land in Northern China/Institute of Agricultural Resources and Regional Planning Chinese Academy of Agricultural SciencesBeijing 100081China Institute of Agricultural Resources and Environment Hebei Academy of Agriculture and Forestry SciencesShijiazhuang 050051China College of Resources and Environment Henan Agricultural UniversityZhengzhou 450002China College of Forestry Hebei Agricultural UniversityBaoding 071000China

Partial substitution of inorganic fertilizers with organic amendments is an important agricultural management *** 11-year field experiment(22 cropping periods)was carried out to analyze the impacts of different partial substitution treatments on crop yields and the transformation of nitrogen fractions in greenhouse vegetable *** treatments with equal N,P_(2)O_(5),and K_(2)O inputs were selected,including complete inorganic fertilizer N(CN),50%inorganic fertilizer N plus 50%pig manure N(CPN),50%inorganic fertilizer N plus 25%pig manure N and 25%corn straw N(CPSN),and 50%inorganic fertilizer N plus 50%corn straw N(CSN).Organic substitution treatments tended to increase crop yields since the 6th cropping period compared to the CN *** the 8th to the 22nd cropping periods,the highest yields were observed in the CPSN treatment where yields were 7.5-11.1%greater than in CN *** 11-year fertilization,compared to CN,organic substitution treatments significantly increased the concentrations of NO_(3)^(-)-N,NH_(4)^(+)-N,acid hydrolysis ammonium-N(AHAN),amino acid-N(AAN),amino sugar-N(ASN),and acid hydrolysis unknown-N(AHUN)in soil by 45.0-69.4,32.8-58.1,49.3-66.6,62.0-69.5,34.5-100.3,and 109.2-172.9%,*** analysis indicated that soil C/N and OC concentration significantly affected the distribution of N *** highest concentrations of NO_(3)^(-)-N,AHAN,AAN,AHUN were found in the CPSN *** substitution treatments increased the activities ofβ-glucosidase,β-cellobiosidase,N-acetyl-glucosamidase,L-aminopeptidase,and phosphatase in the *** substitution treatments reduced vector length and increased vector angle,indicating alleviation of constraints of C and N on soil *** substitution treatments increased the total concentrations of phospholipid fatty acids(PLFAs)in the soil by 109.9-205.3%,and increased the relative abundance of G^(+)bacteria and fungi taxa,but decreased the relat

关键词： organic substitution management greenhouse vegetable production N fractions microbial community composition extracellular enzyme activity enzyme stoichiometry coefficients

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Mesenchymal stromal cells modulate unfolded protein response and preserve β-cell mass in type 1 diabetes

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BIOCELL 2024年第7期48卷 1115-1126页

作者： SIyuan LIU yuan ZHAO YU YU DOU YE QIAN WANG ZHAOYAN WANG ZUO luan Department of Pediatrics The Sixth Medical CenterChinese PLA General HospitalBeijing100037China

Introduction:Transplantation of mesenchymal stromal cells(MSCs)is a promising therapy for type 1 diabetes(T1D).However,whether the infused MSCs affect the endoplasmic reticulum stress or subsequent unfolded protein response inβcells remains ***:To investigate this,we induced early-onset T1D in non-obese diabetic mice using ***,T1D mice were randomly assigned to receive either MSCs or phosphate-buffered *** observed the in vivo homing of MSCs and assessed their effectiveness by analyzing blood glucose levels,body weight,histopathology,pancreatic protein expression,and serum levels of cytokines,proinsulin,and ***:Infused MSCs were found in the lungs,liver,spleen,and pancreas of T1D *** exhibited various effects,including reducing blood glucose levels,regulating immunity,inhibiting inﬂammation,increasingβ-cell areas,and reducing the expression of key proteins in the unfolded protein response *** serum proinsulin and C-peptide levels were signiﬁcantly higher in the MSCs treatment group than in the T1D model ***,there was no signiﬁcant difference in the biomarker ofβ-cell endoplasmic reticulum stress,the ratio of fasting serum proinsulin to C-peptide,between the two ***:Ourﬁndings reveal that MSCs infusion does not alleviate endoplasmic reticulum stress inβcells directly but modulates the unfolded protein response pathway to preserveβ-cell mass and function in T1D mice.

关键词： Type 1 diabetes Mesenchymal stromal cells Endoplasmic reticulum stress Unfolded protein response Non-obese diabetic mice

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Projecting the Dynamic Trends of Human Immunodeficiency Virus/Acquired Immunodeficiency Syndrome:Modeling the Epidemic in Sichuan Province, China

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Biomedical and Environmental Sciences 2024年第9期37卷 1003-1014页

作者： yuan Li Qinxi Liu Rongsheng luan Yi Yang Tao Wu Bihui Yang West China School of Public Health&No.4 West China Teaching Hospital Sichuan UniversityChengdu 610041SichuanChina Chengdu University of Traditional Chinese Medicine Chengdu 611137SichuanChina

Objective Our study aimed to provide a comprehensive overview of the current status and dynamic trends of the human immunodeficiency virus(HIV)prevalence in Sichuan,the second most heavily affected province in China,and to explore future *** The epidemiological,behavioral,and population census data from multiple sources were analyzed to extract inputs for an acquired immunodeficiency syndrome(AIDS)epidemic model(AEM).Baseline curves,derived from historical trends in HIV prevalence,were used,and the AEM was employed to examine future intervention *** In 2015,the modeled data suggested an adult HIV prevalence of 0.191%in Sichuan,with an estimated 128,766 people living with HIV/AIDS and 16,983 individuals with newly diagnosed *** current high-risk behaviors,the model predicts an increase in the adult prevalence to 0.306%by 2025,projecting an estimated 212,168 people living with HIV/AIDS and 16,555 individuals with newly diagnosed *** Heterosexual transmission will likely emerge as the primary mode of AIDS transmission in ***,we anticipate a stabilization in the incidence of AIDS with a concurrent increase in *** comprehensive intervention measures aimed at high-risk groups could effectively alleviate the spread of AIDS in Sichuan.

关键词： HIV/AIDS AIDS Epidemic Model Heterosexual transmission Sichuan province

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ORPA:a fast and efficient phylogenetic analysis method for constructing genome-wide alignments of organelle genomes

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Journal of Genetics and Genomics 2024年第3期51卷 352-358页

作者： Guiqi Bi Xinxin luan Jianbin Yan Shenzhen Branch Guangdong Laboratory of Lingnan Modern AgricultureKey Laboratory of Synthetic BiologyMinistry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhenGuangdong 518124China Zhengzhou Research Base State Key Laboratory of Cotton BiologySchool of Agricultural SciencesZhengzhou UniversityZhengzhouHenan 450001China

Creating a multi-gene alignment matrix for phylogenetic analysis using organelle genomes involves aligning single-gene datasets manually,a process that can be time-consuming and prone to *** HomBlocks pipeline has been created to eliminate the inaccuracies arising from manual *** processing of a large number of sequences,however,remains a time-consuming *** conquer this challenge,we develop a speedy and efficient method called Organelle Genomes for Phylogenetic Analysis(ORPA).ORPA can quickly generate multiple sequence alignments for whole-genome comparisons by parsing the result files of NCBI BLAST,completing the task just in 1 *** increasing data volume,the efficiency of ORPA is even more pronounced,over 300 times faster than HomBlocks in aligning 60 high-plant chloroplast *** phylogenetic tree outputs from ORPA are equivalent to HomBlocks,indicating its outstanding *** to its speed and accuracy,ORPA can identify species-level evolutionary conflicts,providing valuable insights into evolutionary cognition.

关键词： Ultrafast alignment Organelle phylogenomics Phylogenomic conflict Efficient pipeline Multiple sequence alignment pipeline

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