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Altered Taurine Release in Hypothalamus of Aquaporin-4 Knockout Mice

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Chemical Research in Chinese Universities 2010年第3期26卷 406-408页

作者： ZHANG Hong-guo XIAO Ying-hong YANG Long-fei SU Wei-heng guan xin-gang WANG Yu FENG Xue-chao MA Tong-hui Membrane Channel Research Laboratory Northeast Normal University Changchun 130024 P. R. China

Taurine is concentrated in glial cells in the hypothalamus and released in an osmo-dependent manner through volume-sensitive anion channels. Released tanrine acts on glycine receptors on vasopressin neurons to control vasopressin secretion. Water channel AQP4 is abundant in astrocytes in osmosensory areas such as the supraoptic nucleus of hypothalamus. An HPLC-based method was established to quantify taurine release from isolated hypothalamus tissues in wildtype and AQP4 knockout mice. Under the basal condition, there was no difference in taurine release from AQP4^＋/＋ and AQP4^-/- hypothalamuses. Taurine release from AQP4^-/- hypothalamus under hypoosmotic stimulation was significantly lower than that from AQP4^＋/＋ mice. AQP4 expression in the glial cells of the hypothalamus may play an important role in osmoregulation oftaurine release and subsequent vasopressin secretion.

关键词： Aquaporin-4 Taurine Hypothalamus HPLC Precolumn derivatization

Aquaporin-3 Deletion Reduces Glycerol and ATP Content in Mouse Sciatic Nerve

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Chemical Research in Chinese Universities 2010年第6期26卷 955-957页

作者： ZHANG Di GUO Lei ZHU Na SU Wei-heng guan xin-gang YI Fei HAO Feng XIAO Ying-hong LIU Jia LIU Tian-zhou MA Tong-hui Membrane Channel Research Laboratory Northeast Normal University Changchun 130024 P. R. China The Second Hospital of Jilin University Changchun 130041 P. R. China

We reported the expression and function of aquaglyceroporin AQP3 in mouse peripheral nervous ***3 mRNA was identified in freshly isolated mouse sciatic nerve by *** using AQP3 antibody localized the protein expression in the myelin sheathe of sciatic nerve *** primary morpholo-gical evaluation of sciatic nerves from AQP3-knockout and wildtype mice revealed no significant difference,biochemical analysis demonstrated remarkably decreased glycerol and ATP contents in freshly isolated AQP3-knockout sciatic *** study indicates an important role of facilitated glycerol transport mechanism in peripheral nerve energy metabolism.

关键词： Aquaporin Sciatic nerve myelin Glycerol ATP

Tat-GFP融合蛋白的表达纯化及其穿膜活性

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吉林大学学报（医学版） 2014年第4期40卷 725-728,I0001页

作者：关新刚苏维恒于欣佟海滨孙新北华大学生命科学研究中心吉林吉林132013 吉林大学生命科学学院艾滋病疫苗国家工程实验室吉林长春130012 东北师范大学附属中学高中部生物组吉林长春130021

目的:获得具备穿膜活性与绿色荧光蛋白(GFP)标记的Tat-GFP融合蛋白,探讨Tat-GFP在MCF-7细胞中的跨膜转运特性。方法:应用pET-24a-Tat-GFP质粒转化大肠杆菌BL21感受态细胞,检测不同异丙基硫代半乳糖苷(IPTG)浓度(0.5和1.0mmol·L-1)和不... 详细信息

目的:获得具备穿膜活性与绿色荧光蛋白(GFP)标记的Tat-GFP融合蛋白,探讨Tat-GFP在MCF-7细胞中的跨膜转运特性。方法:应用pET-24a-Tat-GFP质粒转化大肠杆菌BL21感受态细胞,检测不同异丙基硫代半乳糖苷(IPTG)浓度(0.5和1.0mmol·L-1)和不同温度(22℃和37℃)诱导融合蛋白的表达情况;利用Ni-IDA树脂亲和纯化Tat-GFP蛋白,利用GFP特异性抗体采用Western blotting法分析洗脱液中的蛋白;激光共聚焦荧光显微镜下检测Tat-GFP融合蛋白的跨膜转运活性。结果:0.5和1.0mmol·L-1 IPTG诱导出的细菌总蛋白中所含的Tat-GFP蛋白量无明显差异;低温(22℃)诱导生产的Tat-GFP蛋白量较37℃更高;Western blotting分析,GFP抗体能够特异性识别PVDF膜上的蛋白,条带灰度与Tat-GFP蛋白上样量有关联;细胞穿膜实验,绿色荧光分布于MCF-7细胞的细胞质和细胞核中。结论:低温诱导时大肠杆菌BL21菌体上清液中Tat-GFP融合蛋白量更高,所生产的Tat-GFP融合蛋白既具备穿膜活性又具有易于检测的绿色荧光。

关键词： Tat-GFP 细胞穿膜肽融合蛋白穿膜活性

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城市排水信息化管理系统的设计与应用

信息技术 2013年第5期37卷 173-176页

作者：管新岗天津大学管理与经济学部天津300073

从排水系统管理需求出发,结合天津临港工业区现状,提出了基于GIS的城市排水智能化专家决策系统的设计方案。通过集成地理信息系统、排水管网模型和泵站优化调度等,实现预测降雨信息,模拟雨污水在排水管网的运行状态,根据预测信息和专家... 详细信息

从排水系统管理需求出发,结合天津临港工业区现状,提出了基于GIS的城市排水智能化专家决策系统的设计方案。通过集成地理信息系统、排水管网模型和泵站优化调度等,实现预测降雨信息,模拟雨污水在排水管网的运行状态,根据预测信息和专家经验分析确定泵站的实时调度方案。所建立的系统为管理人员有效地进行日常管理、泵站调度以及处理突发事件提供科学依据与参考,实现了不同管理部门之间的数据共享,对提高城市排水系统管理的效率和水平具有重要意义。

关键词：城市排水专家决策系统地理信息系统排水管网模型泵站优化调度

Vector meson's spin alignments in high energy reactions

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 5-20页

作者： Jin-Hui Chen Zuo-Tang Liang Yu-gang Ma xin-Li Sheng Qun Wang Key Laboratory of Nuclear Physics and Ion-beam Application (MoE) Institute of Modern PhysicsFudan University Institute of Frontier and Interdisciplinary Science Key Laboratory of Particle Physics and Particle Irradiation (MOE)Shandong University INFN-Firenze Department of Modern Physics University of Science and Technology of China School of Mechanics and Physics Anhui University of Science and Technology

The global spin alignment of vector mesons has been observed by the STAR collaboration at the Relativistic Heavy Ion Collider （RHIC） at Brookhaven National Laboratory （BNL）.It provides a unique opportunity to probe the correlation between the polarized quark and antiquark in the strongly coupled quark-gluon plasma （sQGP） produced in relativistic heavy ion collisions,opening a new window to explore the properties of s *** addition,spin alignments of vector mesons have also been observed in other high-energy particle collisions such as e+e-annihilations at high energies where hadron production is dominated by quark fragmentation *** results obtained are quite different from those obtained in heavy ion collisions where quark coalescence/combination mechanism dominates suggesting strong dependence on hadronization *** comprehensive studies in different hadronization processes are *** this article,we present a brief review of theoretical and experimental advances in the study of vector meson’s spin alignments in a variety of high-energy particle collisions,with emphasis on hadronization mechanisms.

关键词： vector meson spin alignment global polarization relativistic heavy ion collisions fragmentation

乳腺癌细胞来源的细胞膜纳米囊泡制备及靶向特性

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四川大学学报（医学版） 2023年第1期54卷 198-202页

作者：郭冲杨馥旭穆业腾胡楠楠范宇鑫薛晗郭峰霖关新刚北华大学医学技术学院生物医药工程重点实验室吉林132013 台州学院医学院基础医学系台州318000

目的制备乳腺癌细胞来源的细胞膜纳米囊泡(NVs),探讨其基本特性、肿瘤细胞内吞,在荷瘤小鼠模型中的体内分布,探究其肿瘤靶向特性。方法体外培养乳腺癌4T1细胞,通过超速离心法提取细胞膜,利用脂质体挤出仪制备细胞膜纳米囊泡。应用动态... 详细信息

目的制备乳腺癌细胞来源的细胞膜纳米囊泡(NVs),探讨其基本特性、肿瘤细胞内吞,在荷瘤小鼠模型中的体内分布,探究其肿瘤靶向特性。方法体外培养乳腺癌4T1细胞,通过超速离心法提取细胞膜,利用脂质体挤出仪制备细胞膜纳米囊泡。应用动态光散射方法检测囊泡粒径分布,利用透射电子显微镜研究囊泡的形貌特征。通过检测囊泡在磷酸盐缓冲液中的粒径变化分析囊泡的稳定性。应用CCK8法检测不同质量浓度(5、10、20、50和100 mg·L-1)囊泡处理后树突细胞的存活率。荧光显微镜成像检测囊泡在乳腺癌细胞中的内吞。建立皮下乳腺癌荷瘤小鼠模型,将Cy5.5标记的囊泡经尾静脉注射后通过小动物活体成像系统分析其体内分布。结果制备了乳腺癌细胞4T1来源的囊泡,平均粒径为123.2 nm,在透射电镜下呈现为空心的圆球形结构。囊泡在磷酸盐缓冲液中孵育7 d后粒径无明显变化。CCK8结果显示不同囊泡浓度处理后树突细胞存活率均大于90%,荧光显微镜成像显示囊泡能被乳腺癌细胞摄取到细胞内,体内成像结果显示乳腺癌细胞来源的囊泡具有较正常细胞囊泡更高的肿瘤组织蓄积。结论成功制备出4T1细胞来源的NVs,该NVs能被乳腺癌细胞摄取,并展现良好的肿瘤靶向作用。

关键词：细胞膜纳米囊泡肿瘤靶向药物递送

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水通道蛋白1-C末端肽段/GST融合蛋白的原核表达(简报)

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分子细胞生物学报 2008年第1期41卷 81-85页

作者：李晓萌李江杨南扬关新刚张淑芝惠伟丽麻彤辉东北师范大学生命科学学院膜通道实验室长春130024 吉林大学口腔医院长春130041

水通道蛋白（Aquaporin，AQP）是一类选择性高效转运水分子的细胞膜通道蛋白，广泛存在于原核和真核生物细胞的细胞膜上．主要介导自由水分子的被动跨膜转运．对保持细胞内外液环境的稳态平衡起着重要的作用。AQP1是第一个被发现鉴定的... 详细信息

水通道蛋白（Aquaporin，AQP）是一类选择性高效转运水分子的细胞膜通道蛋白，广泛存在于原核和真核生物细胞的细胞膜上．主要介导自由水分子的被动跨膜转运．对保持细胞内外液环境的稳态平衡起着重要的作用。AQP1是第一个被发现鉴定的水通道蛋白．它是1988年Agre等从红细胞膜分离纯化Rh血型多肽时偶然发现的一个分子量为28KD的疏水性跨膜蛋白．称为形成通道整合膜蛋白28（CHIP28）。Agre因此获得了2003年的诺贝尔奖。水通道蛋白AQP1广泛地表达于红细胞、肾、眼、肺，脉络丛和血管内皮，在体液转运中发挥作用。

关键词： AQP1 GST融合蛋白 E.coli

Maintaining moderate levels of hypochlorous acid promotes neural stem cell proliferation and differentiation in the recovery phase of stroke

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Neural Regeneration Research 2025年第3期20卷 845-857页

作者： Lin-Yan Huang Yi-De Zhang Jie Chen Hai-Di Fan Wan Wang Bin Wang Ju-Yun Ma Peng-Peng Li Hai-Wei Pu xin-Yian Guo Jian-gang Shen Su-Hua Qi School of Medical Technology Xuzhou Key Laboratory of Laboratory DiagnosticsXuzhou Medical UniversityXuzhouJiangsu ProvinceChina School of Pharmacy Xuzhou Medical UniversityXuzhouJiangsu ProvinceChina Department of Laboratory Medicine Branch Hospital of Huai’an First People’s HospitalHuai’anJiangsu ProvinceChina Department of Laboratory Medicine the Affiliated Hospital of Xuzhou Medical UniversityXuzhouJiangsu ProvinceChina School of Chinese Medicine The University of Hong KongHong Kong Special Administrative RegionChina

It has been shown clinically that continuous removal of ischemia/reperfusion-induced reactive oxygen species is not conducive to the recovery of late ***,previous studies have shown that excessive increases in hypochlorous acid after stroke can cause severe damage to brain *** previous studies have found that a small amount of hypochlorous acid still exists in the later stage of stroke,but its specific role and mechanism are currently *** simulate stroke in vivo,a middle cerebral artery occlusion rat model was established,with an oxygen-glucose deprivation/reoxygenation model established in vitro to mimic *** found that in the early stage(within 24 hours)of ischemic stroke,neutrophils produced a large amount of hypochlorous acid,while in the recovery phase(10 days after stroke),microglia were activated and produced a small amount of hypochlorous ***,in acute stroke in rats,hypochlorous acid production was prevented using a hypochlorous acid scavenger,taurine,or myeloperoxidase inhibitor,4-aminobenzoic acid *** results showed that high levels of hypochlorous acid(200μM)induced neuronal apoptosis after oxygen/glucose deprivation/***,in the recovery phase of the middle cerebral artery occlusion model,a moderate level of hypochlorous acid promoted the proliferation and differentiation of neural stem cells into neurons and *** suggests that hypochlorous acid plays different roles at different phases of cerebral ischemia/reperfusion *** levels of hypochlorous acid(5 and 100μM)promoted nuclear translocation ofβ-*** transfection of single-site mutation plasmids,we found that hypochlorous acid induced chlorination of theβ-catenin tyrosine 30 residue,which promoted nuclear ***,our study indicates that maintaining low levels of hypochlorous acid plays a key role in the recovery of neurological function.

关键词： cell differentiation cerebral ischemia/reperfusion injury chlorination hypochlorous acid microglia neural stem cell neurogenesis nuclear translocation stroke β-catenin

Hard-core Hall tube in superconducting circuits

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Chinese Physics B 2022年第8期31卷 226-233页

作者： xin guan gang Chen Jing Pan Zhi-Guo Gui Department of Materials and Chemical Engineering Taiyuan UniversityTaiyuan 030032China State Key Laboratory of Quantum Optics and Quantum Optics Devices Institute of Laser SpectroscopyShanxi UniversityTaiyuan 030006China Collaborative Innovation Center of Extreme Optics Shanxi UniversityTaiyuan 030006China Collaborative Innovation Center of Light Manipulations and Applications Shandong Normal UniversityJinan 250358China

The Hall tube as a minimum model to simulate the integer quantum Hall effect is essential for exploring topological physics, while it has not been constructed in the recent developing successfully experiments on superconducting *** this work, we propose a feasible experiment scheme using three legs superconducting circuits with transmon qubits to realize a Hall tube. Then we first investigate its topological properties. Since the time–reversal, particle–hole, and chiral symmetries are all broken for the system, the Hall tube belongs to the A class of the Altland–Zirnbauer *** obtain the corresponding topological phase transition both numerically and analytically. Since the chirality is a key character of the quantum Hall effect, we secondly investigate the chiral physics in the Hall tube. We find the topological protected chiral edge currents and discuss its robustness. Finally, we give the possible experimental observations of the topological state and topological protected chiral edge currents.

关键词： Hall tube superconducting circuits topological phase transition chiral edge currents