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Molecular and biological characterization of melon-infecting squash leaf curl China virus in China

Journal of Integrative Agriculture 2020年第2期19卷 570-577页

作者： WU Hui-jie LI Meng HONG Ni PENG Bin gu qin-sheng Zhengzhou Fruit Research Institute Chinese Academy of Agricultural SciencesZhengzhou 450009P.R.China Huazhong Agricultural University Wuhan 430070P.R.China School of Food and Bioengineering Zhengzhou University of Light IndustryZhengzhou 450000P.R.China

It has been reported that squash leaf curl China virus(SLCCNV)infects some Cucurbitaceae crops except for melon(Cucumis melo L.).A new disease of melon exhibiting severe leaf curl and dwarfing was observed in Hainan Province of *** this study,the pathogen was identified as SLCCNV through biological and molecular *** isolate(SLCCNV-HN)possess a bipartite genome,DNA-A(HM566112.1)with the highest nucleotide identity(99%)to SLCCNV-Hn(MF062251.1)pumpkin and SLCCNV-Hn61(AM260205.1)squash isolates from China,whereas DNA-B(HM566113.1)with the highest nucleotide identity(99%)to SLCCNV-Hn(MF062252.1).Phylogenetic analyses based on the full-length SLCCNV-HN DNA-A and-B sequences indicated that SLCCNV-HN melon isolate is clustered with SLCCNV-Hn pumpkin,SLCCNV-Hn61 and SLCCNV-SY squash isolates from southern China,forming an independent *** clone of SLCCNV-HN was constructed and the melon plants were inoculated and the infection rate is 100%,the systemic symptoms in melon showed identical to those of melon plants infected in ***,melon plants transmission of this virus by Bemisia tabaci with a transmission rate of 95%(19/20)showed leaf curl and dwarf symptoms 15 days post transmission,thereby fulfilling Koch’s *** of genomic organization and phylogenetic trees indicated that SLCCNV-HN melon isolate belongs to the Begomovirus *** the best of our knowledge,this is the first characterization of meloninfecting SLCCNV through its genome,infectious clone and transmission.

关键词： melon squash leaf curl China virus(SLCCNV) genome infectious clone

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Mapping subgenomic promoter of coat protein gene of Cucumber green mottle mosaic virus

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Journal of Integrative Agriculture 2020年第1期19卷 153-163页

作者： LIU Mei LIU Li-ming WU Hui-jie KANG Bao-shan gu qin-sheng Henan Provencial Key Laboratory of Fruit and Cucurbit Biology Zhengzhou Fruit Research InstituteChinese Academy of Agricultural Sciences

Many plant viruses utilize subgenomic RNA as gene expression strategy, therefore mapping subgenomic promoter(SGP) is extremely important for constructing viral vectors. Although Cucumber green mottle mosaic virus(CGMMV)-based virus vectors have been constructed, SGP of the coat protein(CP) has not yet mapped. To this end, we firstly presumed 13 nucleotides upstream of the start codon as the transcription starting site(TSS) as previous study identified by random amplification of c DNA ends(RACE). Secondly, the region from nucleotides –110 to +175 is the putative CP SGP, as predicted, a long stem loop structure by the secondary structure of RNA covering movement protein(MP) and CP. To map the CGMMV CP SGP, we further constructed a series of deletion mutants according to RNA secondary structure prediction. The deletion of TSS upstream significantly enhanced CP transcription when 105 nucleotides were retained before the CP TSS. For the downstream of CP TSS, we analyzed the expression of enhanced green fluorescent protein(EGFP) in a series of vectors with partial deletion of the CGMMV CP and found that the nucleotides from +71 to +91 played a key role in the EGFP expression at the transcription level, while EGFP showed the highest expression level when 160 nucleotides were retained downstream of the CP TSS. To confirm these results, we applied online software MEME to predict the motifs and cis-acting elements in the 466 nucleotides covering the sequences of deletion analysis. Conserved motifs and relative acting elements were in regions in which transcription levels were the highest or enhanced. To our best knowledge, this is the first mapping of CGMMV SGP.

关键词： Cucumber green mottle mosaic virus coat protein subgenomic promoter

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Dot-Blot Hybridization for Detection of Five Cucurbit Viruses by Digoxigenin-Labelled cDNA Probes

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Agricultural Sciences in China 2007年第12期6卷 1450-1455页

作者： MENG Juan gu qin-sheng LIN Shi-ming PENG Bin LIU Li-feng TIAN Yan-ping LI Li Zhengzhou Fruit Research Institute Chinese Academy of Agricultural Sciences Zhengzhou 450009 P.R.China Xiamen Entry-Exit Inspection and Quarantine Bureau Xiamen 361012 P.R.China

Dot-blot hybridization was applied in this paper to detect five viruses infecting cucurbitaceous crops, Zuccini yellow mosaic virus （ZYMV）, Watermelon mosaic virus （WMV）, Cucumber mosaic virus （CMV）, Papaya ringspot viruswatermelon strain （PRSV-W） and Squash mosaic virus （SqMV）, as a good alternative assay in seed health test and epidemiological and transgenic research. Digoxigenin-labelled cDNA probes of the five viruses were synthesized by PCR with the specific primers and applied in dot-blot hybridization to detect five viruses in crude extraction of the infected leaves. And three SqMV probes of different lengths （0.55, 1.6, and 2.7 kb, respectively） were designed to investigate the effect of hybridization. The results showed that the sensitivity for detecting the crude extraction of infected leaves by ZYMV, WMV, CMV, PRSV-W, and SqMV was down to 1：160, 1：160, 1：320, 1：160, and 1：320, respectively. Three SqMV probes of different length showed no differences on the sensitivity and specificity. The digoxigenin-labelled probes prepared by PCR could be used for accurate and rapid identification of 5 viruses infecting cucurbitaceous crops with good stabilities, sensitivities, specificity, and reproducibilifies.

关键词： PCR digoxigenin-labelled cDNA probe dot-blot hybridization ZYMV WMV CMV PRSV-W SqMV

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德才兼修：清代闺秀的琴学活动及其文化参与

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南通大学学报（社会科学版） 2019年第4期35卷 111-117页

作者：顾圣琴南京大学文学院

在清代闺秀文艺繁盛的背景下,闺秀琴学及其活动得到极大的发展。闺秀们或从父母习琴,或从闺塾师、琴师习琴,抚琴成为她们生活中一种有限的排遣与交际行为,而琴则因此成为闺秀们实现文化参与的一种独特媒介。琴学在闺秀群体中,还以女教... 详细信息

在清代闺秀文艺繁盛的背景下,闺秀琴学及其活动得到极大的发展。闺秀们或从父母习琴,或从闺塾师、琴师习琴,抚琴成为她们生活中一种有限的排遣与交际行为,而琴则因此成为闺秀们实现文化参与的一种独特媒介。琴学在闺秀群体中,还以女教、母教、闺教的形式代相传授。对闺秀而言,习琴不仅能怡情遣兴,还能收获天伦之爱、夫妇之情,并籍以友于知音,是其对抗生命困境、实现闺门跨越的一种有效方式。特别是,琴德的“雅”“静”等特点与儒家“幽闲贞静”的女德相合一,使得闺秀琴学在发展闺门才艺而外,更于修身养德上有所成就,隐然成为社会对闺秀的文化期许的一部分:女子习琴即是要“比德于琴”。就此而言,闺秀琴学及其活动实现了儒家的乐教理想,客观上亦参与并推动了清代文化的发展与繁荣。

关键词：琴学闺秀琴德女德女教

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甜瓜坏死斑点病毒山东分离物的基因组

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植物病理学报 2017年第2期47卷 234-239页

作者：吴会杰古勤生中国农业科学院郑州果树研究所河南省果树瓜类生物学重点实验室郑州450009

甜瓜坏死斑点病毒(Melon necrotic spot virus,MNSV)是我国甜瓜病毒新纪录种,主要靠种子和土壤真菌传播。本文以MNSV山东分离物(MNSV-Shandong)为材料,利用RT-PCR和RACE技术获得了MNSV山东分离物基因组,并分析了其基因组成和进化关系。M... 详细信息

甜瓜坏死斑点病毒(Melon necrotic spot virus,MNSV)是我国甜瓜病毒新纪录种,主要靠种子和土壤真菌传播。本文以MNSV山东分离物(MNSV-Shandong)为材料,利用RT-PCR和RACE技术获得了MNSV山东分离物基因组,并分析了其基因组成和进化关系。MNSV-Shandong基因组为4 267 nt,编码5个ORF,参与复制的p29和p89基因分别编码29 kDa和89 kDa蛋白质;p42基因编码的外壳蛋白为42 kDa;p7A和p7B基因编码的移动蛋白均为7 kDa。系统进化树表明MNSV-Shandong与海门分离物(MNSV-HM)聚在一起,与欧洲、部分亚洲及北美洲在一个分支上,与日本分离物距离较远。本文为国内首次报道MNSV山东分离物基因组。

关键词：甜瓜甜瓜坏死斑点病毒基因组系统进化树

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GPS: graph contrastive learning via multi-scale augmented views from adversarial pooling

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Science China(Information Sciences) 2025年第1期68卷 145-158页

作者： Wei JU Yiyang gu Zhengyang MAO Ziyue QIAO Yifang qin Xiao LUO Hui XIONG Ming ZHANG School of Computer Science National Key Laboratory for Multimedia Information ProcessingPeking University Artificial Intelligence Thrust The Hong Kong University of Science and Technology Department of Computer Science University of California

Self-supervised graph representation learning has recently shown considerable promise in a range of fields, including bioinformatics and social networks. A large number of graph contrastive learning approaches have shown promising performance for representation learning on graphs, which train models by maximizing agreement between original graphs and their augmented views(i.e., positive views). Unfortunately, these methods usually involve pre-defined augmentation strategies based on the knowledge of human experts. Moreover, these strategies may fail to generate challenging positive views to provide sufficient supervision signals. In this paper, we present a novel approach named graph pooling contrast(GPS) to address these *** by the fact that graph pooling can adaptively coarsen the graph with the removal of redundancy, we rethink graph pooling and leverage it to automatically generate multi-scale positive views with varying emphasis on providing challenging positives and preserving semantics, i.e., strongly-augmented view and weakly-augmented view. Then, we incorporate both views into a joint contrastive learning framework with similarity learning and consistency learning, where our pooling module is adversarially trained with respect to the encoder for adversarial robustness. Experiments on twelve datasets on both graph classification and transfer learning tasks verify the superiority of the proposed method over its counterparts.

关键词： graph representation learning graph neural networks graph contrastive learning graph augmentations graph pooling

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黄瓜绿斑驳花叶病毒传播方式的研究进展

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中国蔬菜 2015年第1期 13-18页

作者：李俊香古勤生中国农业科学院郑州果树研究所河南郑州450009

黄瓜绿斑驳花叶病毒(Cucumber green mottle mosaic virus,CGMMV)是葫芦科作物重要病毒,通过多种方式如种子、土壤、水、介体和机械接触传播,病害蔓延扩散迅速。该病害在许多国家和地区频繁报道,我国也曾大面积受到为害,尤其是西瓜嫁接... 详细信息

黄瓜绿斑驳花叶病毒(Cucumber green mottle mosaic virus,CGMMV)是葫芦科作物重要病毒,通过多种方式如种子、土壤、水、介体和机械接触传播,病害蔓延扩散迅速。该病害在许多国家和地区频繁报道,我国也曾大面积受到为害,尤其是西瓜嫁接地区,产业损失严重。带毒种子为初侵染源,发生过该病害的地块土壤也是重要的侵染源,发病植株可通过灌溉水以及机械接触传播病毒。为了更好地控制该病害的发生与流行,本文介绍了该病毒传播方式方面的研究进展。

关键词：黄瓜绿斑驳花叶病毒传播方式综述

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四种化学诱抗剂防治黄瓜绿斑驳花叶病毒病的试验初报

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中国蔬菜 2015年第7期 40-44页

作者：李俊香古勤生中国农业科学院郑州果树研究所河南郑州450009

为了筛选出能够诱导瓠瓜产生黄瓜绿斑驳花叶病毒(Cucumber green mottle mosaic virus,CGMMV)病抗性的化学诱抗剂,在瓠瓜幼苗上分别喷施不同浓度的2,1,3-苯并噻二唑(2,1,3-Benzothiodiazole,BTH)、芸薹素内酯(Brassinolide,BL)、壳聚糖(Chitosan Oligosaccharide,CTS)、水杨酸(Salicylic Acid,SA)后,人工摩擦接种CGMMV,评价它们的相对防治效果。结果表明:接种前3 d用0.050 g·L-1 BTH、5.0×10-5 g·L-1 BL、1.0 g·L-1 CTS、0.138 g·L-1 SA处理均可使瓠瓜CGMMV的病情指数降低,其中5.0×10-5 g·L-1 BL防效最佳,达70.38%。进一步研究表明,BL、CTS、SA以喷药后3 d为最佳诱导时间,而BTH为喷药后1 d。4种化学物质均能诱导瓠瓜对CGMMV产生抗性,其抗性差异与诱抗剂种类、浓度及诱导时间相关。

关键词：瓠瓜黄瓜绿斑驳花叶病毒化学诱抗剂诱导抗性

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Exploration of Gas‑Dependent Self‑Adaptive Reconstruction Behavior of Cu_(2)O for Electrochemical CO_(2)Conversion to Multi‑Carbon Products

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Nano-Micro Letters 2025年第3期17卷 280-296页

作者： Chaoran Zhang Yichuan gu Qu Jiang Ziyang sheng Ruohan Feng Sihong Wang Haoyue Zhang Qianqing Xu Zijian Yuan Fang Song State Key Laboratory of Metal Matrix Composites School of Materials Science and EngineeringShanghai Jiao Tong UniversityShanghai 200240People’s Republic of China

Structural reconstruction of electrocatalysts plays a pivotal role in catalytic performances for CO_(2)reduction reaction(CO_(2)RR),whereas the behavior is by far superficially ***,we report that CO_(2)accessibility results in a universal self-adaptive structural reconstruction from Cu_(2)O to Cu@CuxO composites,ending with feeding gas-dependent microstructures and catalytic *** CO_(2)-rich atmosphere favors reconstruction for CO_(2)RR,whereas the CO_(2)-deficient one prefers that for hydrogen evolution *** the assistance of spectroscopic analysis and theoretical calculations,we uncover a CO_(2)-induced passivation behavior by identifying a reductionresistant but catalytic active Cu(I)-rich amorphous layer stabilized by*CO ***,we find extra CO production is indispensable for the robust production of *** inverse correlation between durability and FECO/FEC2H4 is disclosed,suggesting that the selfstabilization process involving the absorption of*CO intermediates on Cu(I)sites is essential for durable *** by this insight,we design hollow Cu_(2)O nanospheres for durable and selective CO_(2)RR electrolysis in producing *** work recognizes the previously overlooked passivation reconstruction and self-stabilizing behavior and highlights the critical role of the local atmosphere in modulating reconstruction and catalytic processes.

关键词： CO_(2)reduction reaction Electrocatalysts Cu_(2)O Reconstruction Self-adaptive electrocatalysis

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MNSV p42与寄主因子3-HIBADH互作

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植物病理学报 2021年第4期51卷 583-591页

作者：吴会杰彭斌康保珊古勤生中国农业科学院郑州果树研究所郑州450009

甜瓜坏死斑点病毒(melon necrotic spot virus,MNSV)是侵染甜瓜的重要病毒之一,为害严重。为了明确MNSV的致病机制,本研究以pGBK-p42为诱饵载体,利用Mating的方法从感染MNSV的甜瓜cDNA文库中筛选出6个与p42互作的寄主因子,进一步利用酵... 详细信息

甜瓜坏死斑点病毒(melon necrotic spot virus,MNSV)是侵染甜瓜的重要病毒之一,为害严重。为了明确MNSV的致病机制,本研究以pGBK-p42为诱饵载体,利用Mating的方法从感染MNSV的甜瓜cDNA文库中筛选出6个与p42互作的寄主因子,进一步利用酵母双杂交系统、双分子荧光互补技术(Bimolecular fluorescence complementation,BiFC)等验证p42与寄主因子3-羟基异丁酸脱氢酶(3-Hydroxyisobutyrate dehydrogenase-like 1,3-HIBADH)的互作,并明确了p42蛋白的s-domain是与3-HIBADH互作的关键区域。此外,利用激光共聚焦显微镜观察发现p42 s-domain与3-HIBADH作用改变了3-HIBADH蛋白在烟草表皮的亚细胞定位,进一步证实p42可与3-HIBADH发生相互作用。该结果为阐明甜瓜响应MNSV侵染的互作机制奠定了基础。

关键词：甜瓜坏死斑点病毒外壳蛋白p42 寄主因子 3-羟基异丁酸脱氢酶互作

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