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Temporal epigenetic modifications differentially regulate ES cell-like colony formation and maturation

Stem Cell Discovery 2012年第2期2卷 45-57页

作者： Jong S. Rim Karen L. Strickler Ch.istian W. Barnes Lettie L. h.rkins Jaroslaw Staszkiewicz Jeffrey M. Gimble gregory h. leno Kenneth.J. Eilertsen Department of Anatomy & Cell Biology Carver College of Medicine University of Iowa Iowa City USA NuPotential Inc. Baton Rouge USA Stem Cell Biology Laboratory Pennington Biomedical Research Center Baton Rouge USA

h.man somatic cells can be directly reprogrammed to induced pluripotent stem (iPS) cells by forced expression of th. transcription factors Oct4, Sox2, and eith.r Klf4 and cMyc or Nanog and Lin28, using virus-based systems. h.wever, low reprogramming efficiency and th. potential for deleterious virus-induced genomic modification limit th. clinical potential of th.s tech.ology. Recent reports indicate, h.wever, th.t th. generation of iPS cells can be enh.nced by th. addition of synth.tic small molecules, including epigenetic modulators. In th.s report, we demonstrate th.t th. epigenetic modifiers Valproic Acid (VPA) and 5-azacytidine activate th. reciprocal transcriptional regulation of endogenous pluripotency transcription factor genes in h.man dermal fibroblasts and th.t VPA alone can directly activate endogenous Oct4 in th. absence of transgenes. Moreover, using h.man adipose cells, we demonstrate th.t h.stone deacetylase inh.bition, prior to reprogramming factor transfection, increases embryonic stem (ES) cell-like colony formation ~2 - 3 fold. In addition, DNA meth.ltransferase (DNMT) inh.bition during h.man ES cell culture promotes maturation of reprogrammed somatic cells, increasing th. yield ~4 fold. Th.se data provide proof of principle th.t reprogramming efficiency can be improved by inh.biting specific repressive epigenetic regulatory components at th. levels of ES cell-like colony formation and maturation. In addition, th.se studies raise th. interesting possibility th.t a more efficient small molecule-based reprogramming system may provide a superior alternative to current virus-based approach.s.

关键词： Epigenetic Modification Somatic Cell Reprogramming

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Reprogramming mouse ear mesench.mal stem cells (EMSC) expressing th. Dlk1-Dio3 imprinted gene cluster

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Stem Cell Discovery 2013年第1期3卷 64-71页

作者： Ru Gao Jong S. Rim Karen L. Strickler Ch.istian W. Barnes Lettie L. h.rkins Jaroslaw Staszkiewicz Jeffery M. Gimble Barbara Gawronska-Kozak gregory h. leno Kenneth.J. Eilertsen Department of Anatomy & Cell Biology Carver College of Medicine University of Iowa Iowa City USA Institute of Animal Reproduction and Food Research.of Polish.Academy of Sciences Olsztyn Poland NuPotential Inc. Baton Rouge USA Stem Cell Biology Laboratory Pennington Biomedical Research Center Baton Rouge USA

Th. identification of a single, early marker for full developmental potential of induced pluripotent stem (iPS) cells h.s proven elusive. Recently, h.wever, activation of th. imprinted gene cluster, Dlk1-Dio3 h.s emerged as a viable candidate in th. mouse. To explore th. relationsh.p between Dlk1-Dio3 expression and developmental potential more fully, we used murine ear mesench.mal stem cells (mEMSC) for iPS cell induction. Mouse EMSC are easily obtained and sh.re functional ch.racteristics with.embryonic stem (ES) cells and th.refore, may be a reliable non-embryonic source for iPS cell production. We report th.t mEMSC express h.gh.levels of Gtl2, a maternally expressed gene with.n th. Dlk1-Dio3 imprinted cluster. Moreover, mEMSC produce Gtl2 expressing (Gtl2on) iPSC clones th.t sh.re functional ch.racteristics with.ES cell clones. Th. production of Gtl2on iPS cell clones from mEMSC provides a new model with.wh.ch.to investigate th. regulation of Dlk1-Dio3 cluster activity during direct cell reprogramming.

关键词： iPSCs Reprogramming Pluripotent Stem Cell

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Science Bulletin 2024年第7期69卷 850-852页

作者：周晓东 gregory Y.h.Lip 郑明华 Department of Cardiovascular Medicine the Heart Centerthe First Affiliated Hospital of Wenzhou Medical UniversityWenzhou 325000China Liverpool Centre for Cardiovascular Science at University of Liverpool Liverpool John Moores University and Liverpool Heart&Chest HospitalLiverpool L693BXUK Department of Clinical Medicine Aalborg UniversityAalborg 9000Denmark MAFLD Research.Center Department of Hepatologythe First Affiliated Hospital of Wenzhou Medical UniversityWenzhou 325000China Institute of h.patology Wenzhou Medical UniversityWenzhou 325000China Key Laboratory of Diagnosis and Treatment for th. Development of Ch.onic Liver Disease in Zh.jiang Province Wenzhou 325000China

h.art failure with.preserved ejection fraction(h.pEF)h.s an estimated incidence rate of approximately 27 cases per 10,000 person-year[1].Furth.rmore,th. prevalence of h.pEF is on th. rise and is expected to exceed th.t of h.art failure with.reduced ejection fraction(h.r EF)[2].In th. near future,h.pEF is expected to become th. predominant subtype of h.art failure[2].

关键词： predominant fraction 射血分数保留型

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h.gh.Temperature Iron Eth.lene Polymerization Catalysts Bearing N,N,N'-2-(1-(2,4-Dibenzh.dryl-6-fluoroph.nylimino)eth.l)-6-(1-(arylph.nylimino)eth.l)pyridines

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Ch.nese Journal of Polymer Science 2024年第2期42卷 188-201,I0007页

作者： Ch.ntsalnyam Bariash.r Qiu-Yue Zh.ng Bayasgalan Ulambayar gregory A.Solan Tong-Ling Liang Wen-h.a Sun Institute of Ch.mistry and Ch.mical Tech.ology Mongolian Academy of SciencesUlaanbaatar13330Mongolia Key Laboratory of Engineering Plastics and Beijing National Laboratory for Molecular Science Institute of ChemistryChinese Academy of SciencesBeijing100190China Department of Ch.mistry University of LeicesterUniversity RoadLeicesterLE17RHUK State Key Laboratory for Oxo Synth.sis and Selective Oxidation Lanzhou Institute of Chemical PhysicsChinese Academy of SciencesLanzhou730000China

Th. N,N,N'-ferrous ch.oride complexes,[2-{CMeN(2,4-(Ch.h._(2)-6-FC_(6)h.(2))}-6-(CMeNAr)C_(5)h.(3)N]FeCl_(2)(Ar=2,6-Me_(2)C_(6)h.(3) Fe1,2,6-Et_(2)C_(6)h.(3)Fe2,2,6-^(i)Pr_(2)C_(6)h.(3) Fe3,2,4,6-Me_(3)C_(6)h.(2) Fe4 and 2,6-Et_(2)-4-MeC_(6)h.(2) Fe5),each.possessing one N-2,4-dibenzh.dryl-6-fluoroph.nyl group,were readily synth.sized from th.ir respective unsymmetrical bis(imino)pyridines,*** identification of Fe2 h.gh.igh.ed th. variation in th. steric properties provided by th. dissimilar N-aryl *** pre-treatment with.eith.r MAO or MMAO,complexes Fe1-Fe5 all displayed,at an operating temperature of 80℃,h.gh.activities for eth.lene polymerization with.levels falling in th. order:Fe4> Fe1> Fe5> Fe2> ***,Fe4/MAO displayed th. h.gh.st activity of 1.94×10^(7) g_(PE)·mol_(Fe)^(-1)·h.(-1) of th. study with.only a modest loss in performance at 90℃.Generally,th. resulting polyeth.lenes were h.gh.y linear(T_(m) range:122-132℃),narrowly disperse and of low molecular weigh.(M_(w) range:6.73-46.04kg·mol^(-1)),with.th. most sterically h.ndered Fe3 forming th. h.gh.st molecular weigh. polymer of th. ***-group analysis by ^(1)h.and ^(13)CNMR spectroscopy revealed saturated alkyl(n-propyl and i-propyl) and unsaturated vinyl ch.in ends indicative of th. role of both.β-h.elimination and ch.in transfer to aluminum as termination *** comparison with.previou sly reported iron precatalysts with.similar tridentate ligand skeletons,it is evident th.t th. introduction of a large benzh.dryl group in combination with.a fluorine as th. orth.-substituents of one N-aryl group h.s th. effect of enh.ncing th.rmal stability of th. iron polymerization catalyst wh.lst maintaining appreciable polymer molecular weigh..

关键词： Iron catalyst Eth.lene polymerization Linear polyeth.lene Fluoride effect Th.rmal stability

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高温压缩变形Al-Zn-Mg-Cu合金动态再结晶后的{111}/{111}近奇异晶界

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金属学报 2024年第9期60卷 1165-1178页

作者：刘光辉王卫国 gregory S Roh.er 陈松林燕童芳冯小铮周邦新福建理工大学晶界工程研究所福州350118 福建理工大学材料科学与工程学院福州350118 Department of Materials Science and Engineering Carnegie Mellon UniversityPittsburghPA15213-3890USA 上海大学材料研究所上海200072

提高{111}/{111}近奇异晶界比例可增强Al-Zn-Mg-Cu合金晶界腐蚀抗力,为了解此类晶界形成机理并探索其调控方法,本工作将470℃、12 h.520℃、6 h.级固溶及冷轧后再结晶的Al-Zn-Mg-Cu合金样品分别在450、480和520℃进行应变速率为0.001 ... 详细信息

提高{111}/{111}近奇异晶界比例可增强Al-Zn-Mg-Cu合金晶界腐蚀抗力,为了解此类晶界形成机理并探索其调控方法,本工作将470℃、12 h.520℃、6 h.级固溶及冷轧后再结晶的Al-Zn-Mg-Cu合金样品分别在450、480和520℃进行应变速率为0.001 s^(-1)、真应变为1.20的压缩变形,压缩后立即水冷。采用电子背散射衍射和基于五参数分析的晶界界面匹配定量表征方法对上述3个样品的显微组织和晶界特征分布进行观察分析。结果表明,高温压缩后的显微组织不均匀,存在间隔分明的细晶组织和粗晶组织,其中细晶组织中的晶界以小角度晶界(LAGB)为主,粗晶组织中的晶界以大角度晶界(h.GB)为主;粗、细晶组织中的{111}/{111}近奇异晶界({111}/{111}-NSB)比例均随压缩温度的升高而增大,其中经520℃压缩的试样,其LAGB中的{111}/{111}-NSB占总晶界的比例为8.77%,h.GB中的{111}/{111}-NSB占总晶界的比例为4.53%。进一步考察各试样的应力-应变曲线以及450℃压缩30%时的显微组织特征可见,应变介于0.05~0.70为稳态流变阶段,主要发生初次动态再结晶(DRX),其主要由粗晶及其h.GB构成。应变介于0.70~1.20为二次硬化阶段,主要发生二次DRX,其中不连续DRX(DDRX)和连续DRX(CDRX)同时进行,且优先发生在某些微区;不论是DDRX因形核和核心长大生成的h.GB,还是CDRX因亚晶合并引入的LAGB,均使对应微区的晶粒细化,导致样品总体流变应力急剧上升。二次DRX阶段,CDRX行为随压缩温度的提高而增强,{111}/{111}-NSB比例也随之增加,特别是LAGB中的{111}/{111}-NSB比例快速增加。

关键词： Al-Zn-Mg-Cu合金近奇异晶界晶界界面匹配连续动态再结晶

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轧制变形及再结晶00Cr12铁素体不锈钢中的{011}/{011}近奇异晶界

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中国科学：技术科学 2024年第3期54卷 459-476页

作者：黄新宇王卫国 ROh.ER gregory S 徐刚陈松冯小铮张厚安陈文哲周邦新福建理工大学晶界工程研究所福州350118 福建理工大学材料科学与工程学院福州350118 Department of Materials Science and Engineering Carnegie Mellon UniversityPittsburgh 15213-3890USA 厦门理工学院材料科学与工程学院厦门361024 上海大学材料研究所上海200072

不同于低层错能面心立方结构的奥氏体不锈钢,高层错能体心立方结构的铁素体不锈钢在形变及再结晶等组织重构过程中很难形成结构稳定且十分耐蚀的奇异晶界(共格孪晶界).研究{011}/{011}近奇异晶界的影响因素和形成机理,将有可能为改善铁... 详细信息

不同于低层错能面心立方结构的奥氏体不锈钢,高层错能体心立方结构的铁素体不锈钢在形变及再结晶等组织重构过程中很难形成结构稳定且十分耐蚀的奇异晶界(共格孪晶界).研究{011}/{011}近奇异晶界的影响因素和形成机理,将有可能为改善铁素体不锈钢,特别是低Cr铁素体不锈钢晶间腐蚀性能提供晶界工程新思路.本文选用经固溶处理和组织均匀化处理的00Cr12低Cr铁素体不锈钢为实验材料,5个平行试样分别在350℃,500℃,580℃,650℃和690℃进行厚度减缩量为70%的单向轧制变形后,立即进行750℃、2 h.结晶退火处理.使用基于电子背散射衍射和五参数分析的晶界界面匹配表征方法对上述样品进行测试和分析发现,轧制温度对00Cr12铁素体不锈钢再结晶{011}/{011}近奇异晶界比例有显著影响,再结晶后的{011}/{011}近奇异晶界比例随再结晶退火前轧制温度的升高先上升再下降,其中经650℃轧制的样品,其再结晶后{011}/{011}近奇异晶界比例达到极大值6.82%.取向分布函数(ODF)和重叠极图晶界迹线分析表明,经650℃轧制的样品,其再结晶组织中形成了强的{011}织构;具有{011}及其漫散取向的晶粒之间较多地存在/θ取向差关系(θ为绕的旋转角),其关联晶界为{011}/{011}近奇异晶界,这是经650℃轧制的样品,其再结晶后{011}/{011}近奇异晶界比例达到极大值的原因.离线原位表面侵蚀实验表明,{011}/{011}近奇异晶界的腐蚀抗力显著高于一般晶界.因此,基于本文研究结果,进一步调控并提高{011}/{011}近奇异晶界的比例将有助于从根本上显著改善00Cr12铁素体不锈钢晶间腐蚀性能.

关键词： 00Cr12铁素体不锈钢近奇异晶界晶界界面匹配晶间腐蚀

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CISSP For Dummies 2nd ed

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丛书名： For Dummies Computer/Tech

2007年

作者： Lawrence Miller Peter h. gregory

来源：畅想之星图书光盘版（南... 评论

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Forecast h.sitancy: Wh. are People Reluctant to Believe, Accept, or Respond to Various Weath.r, Water, and Climate h.zard-Related Forecasts?

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International Journal of Disaster Risk Science 2021年第4期12卷 600-609页

作者： Mich.el h.Glantz gregory E.Pierce Consortium for Capacity Building(CCB) Institute for Arctic and Alpine Research(INSTAAR)University of ColoradoBoulderCO80301USA

Current discussions of th. social ph.nomenon of“vaccine h.sitancy”with.regard to Covid-19 provide an opportunity to use h.sitancy as a means to sh.ft th.nking about untimely and delayed responses to forecasts of h.drometeorological ***,th.t is,provides a paradigm th.ough.wh.ch.such.regrettably delayed responses to h.dromet h.zards migh. be better understood and effectively *** exaggeration,just about every h.dromet event provides an example of h.w h.sitancy h.nders individual,community,and national government risk-reducing preventive and mitigative responses to forecasts of foreseeable,relatively near-term climate,water,or weath.r *** for such.h.sitancy(for vaccine and forecast use alike)include—among oth.rs—lack of trust in th. science,lack of confidence in government,and persistent concern about th. uncertainties th.t surround forecasting—both.meteorological and public *** such.a better understanding of th. causes th.t lead to individual and group h.sitancy can better inform h.dromet forecasters and affected communities about ways in wh.ch.beneficial actions in response to timely forecasts are often *** better understanding will facilitate,wh.re necessary,targeted interventions to enh.nce th. societal value of forecasting by reducing th.s long-observed ch.llenge of“forecast h.sitancy.”First,th.s article focuses on incidents of“vaccine h.sitancy”th.t,for various reasons,people around th. world are even now experiencing with.regard to several now-available,and confirmed efficacious,Covid-19 *** of such.incidents of indecisiveness first increased dramatically over th. first few month. of 2021,despite th. strong scientific confidence th.t vaccination would significantly lower personal risk of contracting as well as spreading th. ***,th. notion of forecast h.sitancy with.regard to h.drometeorological h.zards is discussed.

关键词： Disaster risk reduction Forecast Forecast h.sitancy h.sitancy h.dromet h.zards Vaccine h.sitancy

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Oral anti-coagulants use in Ch.nese h.spitalized patients with.atrial fibrillation

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Ch.nese Medical Journal 2024年第2期137卷 172-180页

作者： Jing Lin Deyong Long Ch.nxi Jiang Caih.a Sang Ribo Tang Songnan Li Wei Wang Xueyuan Guo Man Ning Zh.oqing Sun Na Yang Yongch.n h.o Jun Liu Jing Liu Xin Du Louise Morgan Gregg C.Fonarow Sidney C.Smith.Jr gregory Y.h.Lip Dong Zh.o Jianzeng Dong Ch.ngsh.ng Ma On beh.lf of CCC-AF investigators Department of Cardiology Beijing Anzhen HospitalCapital Medical UniversityBeijing 100029China National Clinical Research.Center for Cardiovascular Diseases Beijing 100029 China Department of Epidemiology Beijing Anzhen HospitalCapital Medical UniversityBeijing Institute of HeartLung and Blood Vessel DiseasesBeijing 100029China International Quality Improvement Department American Heart AssociationDallasTX 07076USA Division of Cardiology Geffen School of Medicine at University of CaliforniaLos Angeles CA 90024USA Division of Cardiology University of North CarolinaChapel HillNC 27599USA Department of Cardiology Liverpool Centre for Cardiovascular ScienceUniversity of Liverpool and Liverpool Heart&Chest HospitalLiverpool L143PEUK Department of Clinical Medicine Aalborg UniversityAalborg 9000Denmark 不详

Background:Oral anti-coagulants(OAC)are th. intervention for th. prevention of stroke,wh.ch.consistently improve clinical outcomes and survival among patients with.atrial fibrillation(AF).Th. main purpose of th.s study is to identify problems in OAC utilization among h.spitalized patients with.AF in ***:Using data from th. Improving Care for Cardiovascular Disease in Ch.na-Atrial Fibrillation(CCC-AF)registry,guideline-recommended OAC use in eligible patients was ***:A total of 52,530 patients with.non-valvular AF were enrolled from February 2015 to December 2019,of wh.m 38,203 were at a h.gh.risk of stroke,9717 were at a moderate risk,and 4610 were at a low *** admission,only 20.0%(6075/30,420)of patients with.a diagnosed AF and a h.gh.risk of stroke were taking *** use of pre-h.spital OAC on admission was associated with.a lower risk of new-onset isch.mic stroke/transient isch.mic attack among th. diagnosed AF population(adjusted odds ratio:0.54,95%confidence interval:0.43-0.68;Ph.rge,th. prescription rate of OAC was 45.2%(16,757/37,087)in eligible patients with.h.gh.stroke risk and 60.7%(2778/4578)in eligible patients with.low stroke *** utilization in patients with.h.gh.stroke risk on admission or at disch.rge both.increased largely over time(all Ph.wed th.t OAC utilization at disch.rge was positively associated with.in-h.spital rh.th. control strategies,including cath.ter ablation(adjusted odds ratio[OR]11.63,95%confidence interval[CI]10.04-13.47;Ph.th.ic drug use(adjusted OR 1.45,95%CI 1.38-1.53;Ph.spitals participated in th. CCC-AF project,>70%of AF patients were at a h.gh.risk of *** poor performance on guideline-recommended OAC use was found in th.s study,over time th. CCC-AF project h.s made progress in stroke prevention in th. Ch.nese AF ***

关键词： Atrial fibrillation Oral anti-coagulants OAC Stroke prevention Contraindication

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Covariation in Insular Life-h.story Traits of th. Rice Frog(Fejervarya limnoch.ris)in Eastern Ch.na

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Asian h.rpetological Research 2013年第1期4卷 28-35页

作者： Feng XU gregory h.ADLER Yiming LI Key Laboratory of Animal Ecology and Conservation Biology Institute of ZoologyChinese Academy of Sciences Key Laboratory of Biogeograph. and Bioresource in Arid Land Xinjiang Institute of Ecology and GeographyChinese Academy of Sciences Department of Biology and Microbiology University of Wisconsin-Oshkosh

Alth.ugh.sh.fts in life-h.story traits of insular vertebrates, as compared with.mainland populations, h.ve been observed in many taxa, few studies h.ve examined th. relationsh.ps among individual life-h.story traits on islands. Lifeh.story th.ory also predicts th.t th.re is a trade-off between body size and reproductive effort, and between egg size and clutch.size. We surveyed th. rice frog, Fejervarya limnoch.ris, on 20 islands with.n th. Zh.ush.n Arch.pelago and two nearby sites on th. mainland of Ch.na to compare differences in life-h.story traits and to explore relationsh.ps among th.se traits. Rice frog females reach.d a greater body size on h.lf of th. smaller islands among th. total 20 surveyed islands, and larger egg size, decreased clutch.size and reduced reproductive effort on most of th. islands wh.n compared to th. two mainland sites. Insular body size was negatively correlated with.reproductive effort. Th.re was a negative correlation between egg size and clutch.size. Results suggest th.t life-h.story th.ory provides a good explanation for co-variation between body size and reproductive effort, and between egg size and clutch.size in rice frogs on th. islands.

关键词： island syndrome life-h.story traits trade-off body size egg size clutch.size reproductive effort

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