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GoldCLIP： Gel-omitted Ligation-dependent CLIP

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Genomics, Proteomics & Bioinformatics 2018年第2期16卷 136-143页

作者： jiaqi Gu Ming Wang Yang Yang Ding Qiu Yiqun Zhang jinbiao Ma Yu Zhou gregory j. Hannon Yang Yu State Key Laboratory of Genetic Engineering Department of Biochemistry School of Life Sciences Fudan University Shanghai 200438 China CAS Key Laboratory of RNA Biology Institute of Biophysics Chinese Academy of Sciences Beijing 100101 China Hubei Key Laboratory of Cell Homeostasis College of Life Sciences Wuhan University Wuhan 430072 China Cancer Research UK Li Ka Shing Centre University of Cambridge Cambridge CB2 ORE United Kingdom

Protein-RNA interaction networks are essential to understand gene regulation control. Identifying binding sites of RNA-binding proteins （RBPs） by the UV-crosslinking and immunoprecipitation （CLIP） represents one of the most powerful methods to map protein RNA interactions in vivo. However, the traditional CLIP protocol is technically challenging, which requires radioactive labeling and suffers from material loss during PAGE-membrane transfer procedures. Here we introduce a super-efficient CLIP method （GoldCLIP） that omits all gel purification steps. This nonisotopic method allows us to perform highly reproducible CLIP experiments with polypyrimidine tract-binding protein （PTB）, a classical RBP in human cell lines. In principle, our method guarantees sequencing library constructions, providing the protein of interest can be successfully crosslinked to RNAs in living cells. GoldCLIP is readily applicable to diverse proteins to uncover their endogenous RNA targets.

关键词： RNA binding protein UV crosslinking CLIP HaloTag PTB

Variation in resource competition traits among Microcystis strains is affected by their microbiomes

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mLife 2023年第4期2卷 401-415页

作者： Dylan Baker Casey M.Godwin Muhtamim Khanam Ashley M.Burtner gregory j.Dick Vincent j.Denef Department of Ecology and Evolutionary Biology University of MichiganAnn ArborMichiganUSA Cooperative Institute for Great Lakes Research School for Environment and SustainabilityUniversity of MichiganAnn ArborMichiganUSA Department of Earth and Environmental Sciences University of MichiganAnn ArborMichiganUSA

Freshwater harmful algal blooms are often dominated by Microcystis,a phylogenetically cohesive group of cyanobacteria marked by extensive genetic and physiological *** have previously shown that this genetic diversity and the presence of a microbiome of heterotrophic bacteria influences competitive interactions with eukaryotic *** this study,we sought to explain these observations by characterizing Monod equation parameters for resource usage(maximum growth rateμmax,half-saturation value for growth Ks,and quota)as a function of N and P levels for four strains(NIES-843,PCC 9701,PCC 7806[WT],and PCC 7806ΔmcyB)in presence and absence of a microbiome derived from Microcystis isolated from Lake *** indicated limited differences in maximum growth rates but more pronounced differences in half-saturation values among Microcystis *** largest impact of the microbiome was reducing the minimal nitrogen concentration sustaining growth and reducing half saturation values,with variable results depending on the Microcystis *** strains also differed from each other in their N and P quotas and the extent to which microbiome presence affected *** data highlight the importance of the microbiome in altering Microcystis-intrinsic traits,strain competitive hierarchies,and thus bloom *** quota,μmax,and Ks are commonly used in models for harmful algal blooms,our data suggest that model improvement may be possible by incorporating genotype dependencies of resource-use parameters.

关键词： cultivation-dependent fitness harmful algal blooms host-microbe interactions phytoplankton

Circumcision of Infants and Children: Short-Term Trauma and Long-Term Psychosexual Harm

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Advances in Sexual Medicine 2015年第2期5卷 22-38页

作者： gregory j. Boyle Australian Institute of Psychology Fortitude Valley Queensland Australia

Non-therapeutic infant male circumcision is a permanent surgical alteration to the penis that may cause significant physical, sexual and psychological harm. Physical harms include unintended adverse effects of the surgery itself (e.g., complications such as bleeding, infection, excessive removal of foreskin leaving insufficient shaft skin to accommodate erections, etc.), as well as the inherent loss of healthy, functional tissue. Sexual harms that necessarily follow from circumcision include the loss of all sensation in the foreskin itself, and the loss of all sexual functions that involve the physical manipulation of the foreskin. Additional sexual harms that may follow circumcision include reduced sexual sensation in the remaining penile structures, difficulty with masturbation, increased chafing in both the circumcised man and his sexual partner, as well as reduced overall psychosexual/psychological tension relief and subjective satisfaction. Psychological harms include short-term trauma as well as the potential for long-term emotional disturbances, including sadness, frustration, distress, and anger—akin to post-traumatic stress disorder (PTSD). In this paper, the extent and severity of these various harms are considered and it is argued that they are more serious and more widespread than is commonly believed.

关键词： Circumcision Sexual Reduction Surgery Circumcision-Related Deaths Male Genital Mutilation

Does Male Circumcision Adversely Affect Sexual Sensation, Function, or Satisfaction? Critical Comment on Morris and Krieger (2013)

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Advances in Sexual Medicine 2015年第2期5卷 7-12页

作者： gregory j. Boyle Australian Institute of Psychology Fortitude Valley Queensland Australia

Morris and Krieger (2013) have argued that male circumcision does not impact adversely on sexual sensation, satisfaction, and/or function. In the present paper, it is argued that such a view is untenable. By selectively citing Morris’ own non-peer-reviewed letters and opinion pieces purporting to show flaws in studies reporting evidence of negative effects of circumcision, and by failing adequately to account for replies to these letters by the authors of the original research (and others), Morris and Krieger give an incomplete and misleading account of the available literature. Consequently, Morris and Krieger reach an implausible conclusion that is inconsistent with what is known about the anatomy and functions of the penile foreskin, and the likely effects of its surgical removal.

关键词： Circumcision Sexual Dysfunction

China＇s ＂Peaceful Rise＂： Rhetoric or Reality7

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Fudan journal of the Humanities and Social Sciences 2010年第4期3卷 132-137页

作者： gregory j. Moore

With the astonishing growth in China＇s economy over the past thirty years and the concomitant growth in China＇s power and influence in international affairs have come a number of new ＂China＇s rise＂ books, for very understandable reasons. Among the more notable examples are those by Taylor Fravel, David Kang and David （Mike） Lampton,

关键词：中国经济发展发展战略市场分析

Realism on China＇s Rise： The Limits of Harmony （ and War）

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Fudan journal of the Humanities and Social Sciences 2013年第2期6卷 143-150页

作者： gregory j. Moore

Wang Yuan-kang has written an important book on China＇s historical foreign policy and the implications of these historical arguments on China＇s present day policies. Titled, Harmony and War： Confucian Culture and Chinese Power Politics （Columbia University Press, 2010）, Wang＇s volume compares two approaches to understanding China＇s historical foreign policy： Confucian pacifism, and structural Realism;

关键词：中国历史现实主义战争和谐哥伦比亚大学外交政策儒家文化出版社

Proteasome inhibition-induces endoplasmic reticulum dysfunction and cell death of human cholangiocarcinoma cells

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World journal of Gastroenterology 2007年第6期13卷 851-857页

作者： Yucel Ustundag Steven F Bronk gregory j Gores Mayo Clinic College of Medicine Rochester MN 55905 United States

AIM: To determine if proteasome inhibition induces apoptosis in human cholangiocarcinoma cells, and if so, to elucidate the cellular mechanisms. METHODS: Studies were performed in the human KMCH, KMBC, and Mz-ChA-1 cholangiocarcinoma, and normal rat cell lines. MG132, a peptide aldehyde, which inhibits the chymotrypsin-like activity of the proteaosome was employed for this study. Apoptosis was assessed morphologically by 4'-6-Diamidino-2-phenylindole (DAPI) nuclear staining and fluorescence microscopy. Mitochondrial membrane potential was examined using a fluorescent unquenching assay. Ultrastructural changes during cell death were examined using transmission electron microscopy (TEM). Caspase 3/7 activity was assessed using an enzymatic-based fluorescent assay. Cytosolic-free calcium concentrations were measured using Fura-2 and digitized fluorescent microscopy. RESULTS: MG132, a proteasome inhibitor, induced apoptosis in all the cholangiocarcinoma cell lines examined. In contrast, minimal cytotoxicity was observed in normal rat cholangiocytes. Apoptosis was time-and -concentration-dependent. There was no change in the mitochondrial membrane potential between treated and untreated cells. Ultrastructural examination by transmission electron microscopy displayed the classic features of apoptosis, but in addition, there was also dramatic vacuolization of the endoplasmic reticulum (ER). Unexpectedly, no increase in caspase 3/7 activity was observed in MG132 treated cells, nor did the pancaspase inhibitor, Q-VD-OPh prevent cell death. The protein synthesis inhibitor, cycloheximide, blocked apoptosis induced by proteosome inhibitor indicating that ER dysfunction was dependent upon the formation of new proteins. CONCLUSION: Proteosome inhibition induces ERdysfunction and caspase-independent cell death selectively in human cholangiocarcinoma cells. Proteasome inhibitors warrant evaluation as anticancer agents for the treatment of human cholangiocarcinoma.

关键词： MG132 Cholangiocarcinoma Proteosome Apoptosis Calcium

Short history of Harry *** MD

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中华显微外科杂志 2020年第1期43卷 I0009-I0010页

作者： gregory M.Buncke 不详

我的父亲哈里本克(Harry ***),被公认为“显微外科之父”。他在格拉斯哥医务室作为专科培训生、师从托马斯吉布森(Thomas Gibson)教授时,便开始对修复直径1 mm的血管感兴趣.在参与Gibson教授的移植免疫学工作以及缩短分离蒂转移时间... 详细信息

我的父亲哈里本克(Harry ***),被公认为“显微外科之父”。他在格拉斯哥医务室作为专科培训生、师从托马斯吉布森(Thomas Gibson)教授时,便开始对修复直径1 mm的血管感兴趣.在参与Gibson教授的移植免疫学工作以及缩短分离蒂转移时间的研究中,Buncke博士便设想仅通过一次手术将营养动脉/静脉修复到受体部位的皮瓣来移植同源组织,从而避免附着皮瓣。

关键词：受体部位专科培训移植免疫学显微外科医务室吉布森托马斯

Diabetes exacerbates inflammatory bowel disease in mice with dietinduced obesity

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世界胃肠病学杂志:英文版 2023年第33期29卷 4991-5004页

作者： Kendra L Francis Kimberly M Alonge Maria Cristina Pacheco Shannon j Hu Cody A Krutzsch gregory j Morton Michael W Schwartz jarrad M Scarlett Department of Pediatric Gastroenterology and Hepatology Seattle Children’s HospitalSeattleWA 98105United States 不详 Department of Medicinal Chemistry University of WashingtonSeattleWA 98195United States Department of Laboratory Medicine and Pathology Seattle Children's HospitalSeattleWA 98105United States

BACKGROUND The increased prevalence of inflammatory bowel disease(IBD)among patients with obesity and type 2 diabetes suggests a causal link between these diseases,potentially involving the effect of hyperglycemia to disrupt intestinal barrier *** To investigate whether the deleterious impact of diabetes on the intestinal barrier is associated with increased IBD severity in a murine model of colitis in mice with and without diet-induced *** Mice were fed chow or a high-fat diet and subsequently received streptozotocin to induce diabetic-range *** weeks later,dextran sodium sulfate was given to induce *** select experiments,a subset of diabetic mice was treated with the antidiabetic drug dapagliflozin prior to colitis *** included both clinical and histological measures of colitis activity as well as histochemical markers of colonic epithelial barrier *** In mice given a high-fat diet,but not chow-fed animals,diabetes was associated with significantly increased clinical colitis activity and histopathologic markers of disease *** was also associated with a decrease in key components that regulate colonic epithelial barrier integrity(colonic mucin layer content and epithelial tight junction proteins)in diet-induced obese *** of these effects of diabetes in diet-induced obese mice was ameliorated by restoring *** In obese mice,diabetes worsened clinical and pathologic outcomes of colitis via mechanisms that are reversible with treatment of ***-induced intestinal barrier dysfunction offers a plausible mechanism linking diabetes to increased colitis *** findings suggest that effective diabetes management may decrease the clinical severity of IBD.

关键词： Inflammatory bowel disease Type 2 diabetes Obesity Intestinal barrier Hyperglycemia Colitis in mice Tight junction proteins