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Imidazolium-based ionic liquids as additives to preserve the Enhanced Green Fluorescent Protein fluorescent activity

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Green Chemical Engineering 2021年第4期2卷 412-422页

作者： Nathalia V.Veríssimo Carolina F.Saponi Timothy m.Ryan Tamar L.greaves Jorge F.B.Pereira Department of Engineering of Bioprocesses and Biotechnology School of Pharmaceutical SciencesSao Paulo State University(UNESP)Rodovia Araraquara-Jaú/Km 0114800-903AraraquaraSPBrazil School of Science College of ScienceEngineering and HealthRMIT University124 La Trobe StreetMelbourneVIC 3000Australia School of Pharmaceutical Sciences Sao Paulo University(USP)Av.Prof.Lineu Prestesno.580B1605508-000Cidade de UniversitariaSao PauloSPBrazil Australian Synchrotron 800 Blackburn RoadClaytonVIC 3168Australia Univ Coimbra CIEPQPFDepartment of Chemical EngineeringRua Sílvio LimaPolo II–Pinhal de Marrocos3030-790 CoimbraPortugal

Fluorescent Proteins(FP)can be applied as biomarkers and biosensors in the industrial and medical fields,but their large-scale use,especially for new industrial applications,is limited due to their low ***,the discovery of additives capable of preserving the activity of FP at room temperature and under stress conditions can help to expand and facilitate their commercial *** this goal,we evaluated the application of 1-alkyl-3-methylimidazolium chloride-based([C_(n)mim]Cl)ionic liquids(ILs)as additives to preserve the activity of Enhanced Green Fluorescent Protein(EGFP)at different storage times and under unfavorable ***[C_(n)mim]Cl solutions(at 0.100 mol L^(-1))were able to preserve EGFP fluorescence for longer than the phosphatesaline buffer(PBS)and NaCl solutions,increasing its fluorescence manifestation from 1 to 3 months.[C_(n)mim]Cl with shorter to medium cationic alkyl chains were the most effective in preserving EGFP fluorescence.[C_(n)mim]Cl also protected EGFP activity in the presence of the surfactant SDS,the acid guanidine hydrochloride,and H_(2)O_(2).Therefore,[C_(n)mim]Cl can be added to aqueous solutions to preserve EGFP fluorescence activity at room temperature for longer storage times and to reduce the negative impact of denaturing agents on ***,there is a massive potential for the application of ILs as additives to preserve FP in the long-term.w.thout refrigeration and under unfavorable conditions,and this is fundamental to enable expansion of FP in industrial and commercial applications.

关键词： Green Fluorescent Protein Protein stability Fluorescence Ionic liquids Preservatives

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注空气低温氧化驱油室内实验与油藏筛选标准

油气地质与采收率 2008年第1期15卷 69-71页

作者：王杰祥徐国瑞付志军任韶然 greaves m 中国石油大学(华东)石油工程学院山东东营257061 英国Bath大学英国巴斯市

对于低渗透油藏和注水开发后期油藏来说,注空气低温氧化提高采收率技术是一种经济、有效的方法。通过注空气低温氧化静态实验和注空气动态驱油实验,进行注空气技术可行性研究,评价不同油品的低温氧化性能,结合应用实例,建立了注空气目... 详细信息

对于低渗透油藏和注水开发后期油藏来说,注空气低温氧化提高采收率技术是一种经济、有效的方法。通过注空气低温氧化静态实验和注空气动态驱油实验,进行注空气技术可行性研究,评价不同油品的低温氧化性能,结合应用实例,建立了注空气目标油藏筛选标准,如油藏温度、油藏厚度、原油性质等。产出气中氧气的体积分数低于5%的实验结果表明,开展注空气提高采收率技术安全、有效,具有推广应用的价值。

关键词：注空气低温氧化实验研究油藏筛选评价方法

Neonatal neuroimaging after repair of congenital diaphragmatic hernia and long-term neurodevelopmental outcome

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world Journal of Pediatric Surgery 2019年第3期2卷 2-8页

作者： Julia Kate Gunn-Charlton Alice C Burnett Stephanie malarbi margaret m moran Esther A Hutchinson Susan greaves Rod w Hunt Newborn Intensive Care The Royal Children’s HospitalMelbourneVictoriaAustralia Neonatal Research Group Murdoch Children’s Research InstituteMelbourneVictoriaAustralia Department of Paediatrics The University of MelbourneMelbourneVictoriaAustralia Victorian Infant Brain Studies(VIBeS)Group Murdoch Children's Research InstituteMelbourneVictoriaAustralia Department of Occupational Therapy The Royal Children’s HospitalMelbourneVictoriaAustralia

Objective Previous outcome reports of congenital diaphragmatic hernia(CDH)have described neuroimaging anomalies and neurodevelopmental ***,the link between imaging and outcome has not been *** aimed to determine whether routine postoperative neonatal neuroimaging in infants with CDH detects later neurodevelopmental *** In a prospective cohort study within a clinical service in The Royal Children’s Hospital Newborn Intensive *** ultrasound was performed in 81 children and mRI in 57 children who subsequently underwent neurodevelopmental follow-up after surgery for *** scans were analyzed using a scoring system designed to identify injury,maturation and volume *** assessment occurred at 2 years(48)and neurocognitive assessment at 5 years(26)and/or 8 years(27).Brain imaging scores corrected for gestational age at scan time were correlated with outcome measures,adjusting for known clinical *** Clinically significant findings were identified on mRI of 16(28%)*** scores were in the normal range for all domains assessed at each *** impairment was seen in 23%at 2 years and verbal intellectual impairment in 25%at 8 *** cognitive scores were lower in 2-year-old children with white matter injury on mRI(p=0.03).mean motor scores were lower in 2-year-old children with brain immaturity(p=0.01).Associations between mRI and 5-year and 8-year assessments were no longer significant when adjusting for known clinical *** Neuroimaging abnormalities were associated with worse neurodevelopment at 2 years,but not with later neurocognitive outcomes,after accounting for clinical risk factors.

关键词： hernia routine maturity

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开采稠油并原地提高原油品位的新技术

特种油气藏 2002年第1期9卷 75-76页

作者： m greaves 高益桁张冰马雁中油辽河油田分公司勘探开发研究院辽宁盘锦124010

引言THAI(' Toe-to-Heel' Air Injection)水平段注空气技术是一项重力辅助的火烧油层工艺.它结合了水平井先进技术以获得潜在的、极高的稠油采收率;通过热裂化实现原地提高原油品位、开采出改质的原油.该工艺利用重力稳定性原理限制向... 详细信息

引言THAI(' Toe-to-Heel' Air Injection)水平段注空气技术是一项重力辅助的火烧油层工艺.它结合了水平井先进技术以获得潜在的、极高的稠油采收率;通过热裂化实现原地提高原油品位、开采出改质的原油.该工艺利用重力稳定性原理限制向狭窄的可流动区域泄油,这样就可以使已经处于流动状态的流体直接进入生产井的裸露井段.该工艺可用于一次采油、作为现代技术的配套技术或作为热效率要求高的工艺的配套工艺.该技术能够凝聚能量,使原油在油藏中流动、采出和热力升级.THAI中还结合了清洁技术.因此,该技术为稠油工业提供一条新的、成功的路.

关键词：稠油开采原油品位 THAT 水平段注空气技术

Investigation of wave-Structure Interaction Using State of the Art CFD Techniques

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Open Journal of Fluid Dynamics 2014年第1期4卷 18-43页

作者： Jan westphalen Deborah m. greaves Alison Raby Zheng Zheng Hu Derek m. Causon Clive G. mingham Pourya Omidvar Peter K. Stansby Benedict D. Rogers School of Computing Mathematics and Digital Technology Manchester Metropolitan University Manchester UK School of marine Science and Engineering Marine Institute University of Plymouth Plymouth UK School of mechanical Aerospace and Civil Engineering University of Manchester Manchester UK

The suitability of computational fluid dynamics (CFD) for marine renewable energy research and development and in particular for simulating extreme wave interaction with a wave energy converter (wEC) is considered. Fully nonlinear time domain CFD is often considered to be an expensive and computationally intensive option for marine hydrodynamics and frequency-based methods are traditionally preferred by the industry. However, CFD models capture more of the physics of wave-structure interaction, and whereas traditional frequency domain approaches are restricted to linear motions, fully nonlinear CFD can simulate wave breaking and overtopping. Furthermore, with continuing advances in computing power and speed and the development of new algorithms for CFD, it is becoming a more popular option for design applications in the marine environment. In this work, different CFD approaches of increasing novelty are assessed: two commercial CFD packages incorporating recent advances in high resolution free surface flow simulation;a finite volume based Euler equation model with a shock capturing technique for the free surface;and meshless Smoothed Particle Hydrodynamics (SPH) method. These different approaches to fully nonlinear time domain simulation of free surface flow and wave structure interaction are applied to test cases of increasing complexity and the results compared with experimental data. Results are presented for regular wave interaction with a fixed horizontal cylinder, wave generation by a cone in driven vertical motion at the free surface and extreme wave interaction with a bobbing float (The manchester Bobber wEC). The numerical results generally show good agreement with the physical experiments and simulate the wave-structure interaction and wave loading satisfactorily. The grid-based methods are shown to be generally less able than the meshless SPH to capture jet formation at the face of the cone, the resolution of the jet being grid dependent.

关键词： wave Loading wave Energy wave-Structure Interaction manchester Bobber CFD Physical Experiments FV CV-FE SPH Cartesian-Cut-Cell SPHysics AmAZON SC STAR CCm+ CFX

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肝素使用和监控指南

血栓与止血学 2006年第1期12卷 42-48页

作者： Baglin T Barrowcliffe Tw Coben A greaves m 陈君敏曾开淇英国血液学标准委员会福建医科大学第一附属医院福州350000

肝素是天然氨基葡聚糖，由肥大细胞产生。药用的肝素由猪或牛的黏膜中提取。肝素由糖醛酸链和葡糖胺链交替组成，含多少不等的硫酸根，相对分子质量5ku-35ku。现在使用的制剂平均相时分子质量（mw）为13ku～15ku，比活性180—220u／mg。

关键词：肝素监控分子质量肥大细胞

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对遗传性易栓症检查与治疗的指导意见

国外医学（输血及血液学分册） 2004年第6期27卷 484-487页

作者： walker ID greaves m Preston FE 王兆钺英国血液学会

国际上对易栓症尚无统一的定义。英国血液学会标准化委员会提出，易栓症是指“易导致血栓形成的止血机理异常”。这个定义被广泛地采用，但也有两点不足之处：①随着新的易栓状态不断发现，很多有这类异常的人并无症状；②虽然已认识到... 详细信息

国际上对易栓症尚无统一的定义。英国血液学会标准化委员会提出，易栓症是指“易导致血栓形成的止血机理异常”。这个定义被广泛地采用，但也有两点不足之处：①随着新的易栓状态不断发现，很多有这类异常的人并无症状；②虽然已认识到各种能增加血栓危险的异常，但至少50%有血栓病史的患者经详细的实验室检查后仍找不到原因。在北美地区医生将易栓症患者限定为有自发性静脉血栓或其严重性比预期的高，或反复性静脉血栓发作，或在年青时就有静脉血栓的人。这个概念可能在临床上更有用。

关键词：遗传性易栓症治疗诊断实验室检查抗凝血酶缺陷

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细胞识别

细胞识别

作者： m.F.·格里夫斯(m.F.c greaves)

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水平井的火烧油层技术

石油石化节能与计量 1997年第12期 5-7页

作者： m．greaves,O．AL-SHAmALI,郭秀文,许宁辽河油田勘探开发研究院辽河油田勘探开发研究院

使用wolfLake的稠油，在0．4m×0．4m×0．1m的三维矩形燃烧器中进行了一系列干式燃烧和一次湿式燃烧实验。使用的三种不同配置是：①VIHP──一口垂直注入井和一口水平采油井；②HIHP──一口水平注入井和一口与之垂直的水平生产井；... 详细信息

使用wolfLake的稠油，在0．4m×0．4m×0．1m的三维矩形燃烧器中进行了一系列干式燃烧和一次湿式燃烧实验。使用的三种不同配置是：①VIHP──一口垂直注入井和一口水平采油井；②HIHP──一口水平注入井和一口与之垂直的水平生产井；③平行HIHP──一口水平注入井和一口与之平行的水平生产井。所有水平生产井都按排状驱油排列。实验用了含高稠油、饱和度S0≈80％的充填砂层，注入气（空气或含氧35％的富氧空气），记录到的火烧油层最高温度对干式燃烧是747℃，对湿式燃烧是531℃，得到了很高的采收率。注入井的类型和相对于水平生产井的应置对点火后开始形成的燃烧前缘宽度有很大影响。在水平注入井与生产井垂直的情况下（HIHP），形成的燃烧前缘跨越了整个填充砂层宽度。

关键词：水平井火烧油层技术三维物理模型采收率干式燃烧