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High-quality reference genes for quantifying the transcriptional responses of Oryza sativa L. (ssp. indica and japonica) to abiotic stress conditions

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Chinese Science Bulletin 2013年第16期58卷 1919-1930页

作者： MAKSUP Sarunyaporn SUPAIBULWATANA Kanyaratt selvaraj gopalan Department of Biotechnology Faculty of Science Mahidol University Plant Biotechnology Institute National Research Council of Canada

Rice (Oryza sativa L.) is important to food security and is also an excellent model plant for numerous cereal crops. A functional genomics study in rice includes characterization of the expression dynamics of genes by quantitative real-time PCR (qPCR) analysis; this is a significant key for developing rice varieties that perform well in the face of adverse climate change. The qPCR analysis requires the use of appropriate reference genes in order to make any quantitative interpretations meaningful. Here, the new potential reference genes were selected from a huge public database of rice microarray experiments. The expression stability of 14 candidates and 4 conventional reference genes was validated by geNorm PLUS and NormFinder software. Seven candidates are superior to the conventionally used reference genes in qPCR and three genes can be used reliably for quantitating the expression of genes involved in abiotic stress responses. These high-quality references EP (LOC_Os05g08980), HNR (LOC_Os01g71770), and TBC (LOC_Os09g34040) worked very well in three indica genotypes and one japonica genotype. One of indica genotypes including the Jasmine rice, KDML105 developed in Thailand for which no reference genes have been reported until now.

关键词：功能基因组学胁迫反应非生物水稻胁迫条件籼稻品质实时定量PCR

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Analysis of Gene Expression Patterns during Seed Coat Development in Arabidopsis

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Molecular Plant 2011年第6期4卷 1074-1091页

作者： Gillian Dean YongGuo Cao DaoQuan Xiang Nicholas J. Provart Larissa Ramsay Abdul Ahad Rick White gopalan selvaraj Raju Datla George Haughn Department of Botany University of British Columbia 6270 University Blvd Vancouver BC V6T 1Z4 Canada National Research Council of Canada Plant Biotechnology Institute 110 Gymnasium Place Saskatoon SK S7N owg Canada Department of Cell and Systems Biology/Centre for the Analysis of Genome Evolution and Function University of Toronto 25 Willcocks St Toronto ON M5S 3B2 Canada Department of Statistics University of British Columbia 6356 Agricultural Road Vancouver BC V6T 1Z2 Canada

The seed coat is important for embryo protection, seed hydration, and dispersal. Seed coat composition is also of interest to the agricultural sector, since it impacts the nutritional value for humans and livestock alike. Although some seed coat genes have been identified, the developmental pathways controlling seed coat development are not completely elucidated, and a global genetic program associated with seed coat development has not been reported. This study uses a combination of genetic and genomic approaches in Arabidopsis thaliana to begin to address these knowledge gaps. Seed coat development is a complex process whereby the integuments of the ovule differentiate into specialized cell types. In Arabidopsis, the outermost layer of cells secretes mucilage into the apoplast and develops a secondary cell wall known as a columella. The layer beneath the epidermis, the palisade, synthesizes a secondary cell wall on its inner tangential side. The innermost layer （the pigmented layer or endothelium） produces proanthocyanidins that condense into tannins and oxidize, giving a brown color to mature seeds. Genetic separation of these cell layers was achieved using the ap2-7 and tt16-1 mutants, where the epidermis/palisade and the endothelium do not develop respectively. This genetic ablation was exploited to examine the developmental programs of these cell types by isolating and collecting seed coats at key tran- sitions during development and performing global gene expression analysis. The data indicate that the developmental programs of the epidermis and the pigmented layer proceed relatively independently. Global expression datasets that can be used for identification of new gene candidates for seed coat development were generated. These dataset provide a comprehensive expression profile for developing seed coats in Arabidopsis, and should provide a useful resource and reference for other seed systems.

关键词： Seed coat microarray APETALA2 TRANSPARENT TESTA16 mucilage pectin secondary cell wall pigmentedlayer proanthocyanidin.

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The Molecular Basis of Plant Penetration Resistance to Fungal Pathogens

The Molecular Basis of Plant Penetration Resistance to Funga...

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中国植物病理学会2011年学术年会

作者： Nazmul Bhuiyan David Greenshields John King gopalan selvaraj Department of Biology College of Arts and ScienceUniversity of SaskatchewanSaskatoonSK S7N 5E2Canada

Resistance to fungal penetration of the epidermal cell wall is a key component of plant basal *** resistance depends on reinforcement of the cell wall at the site of attempted penetration through the development of specialized cell wall appositions(papilla formation),typically containing callose,cell wall cross-linked proteins,polyphenolics (lignin),iron(Fe),and hydrogen *** use monocot cereals and dicot Arabidopsis to identify molecular components involved in papilla ***

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Management practices and technologies for efficient biological sample collection from domestic animals with special reference to Indian field conditions

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Animal Diseases 2023年第4期3卷 242-255页

作者： Gokula Kannan Ragavan Purushothaman selvaraj Dharman Murugesan Tirumurugaan Krishnaswamy gopalan Soundararajan Chinnaiyan Dhinakar Raj Gopal Aravindh Babu Ramasamy Parthiban Karthik Kumaragurubaran Azhahianambi Palavesam Translational Research Platform for Veterinary Biologicals Centre for Animal Health StudiesTamil Nadu Veterinary and Animal Sciences UniversityChennaiTamil Nadu 600051India Centre for Animal Health Studies Tamil Nadu Veterinary and Animal Sciences UniversityChennaiTamil Nadu 600051India Department of Animal Biotechnology Madras Veterinary CollegeVeperyChennaiTamil Nadu 600007India Department of Veterinary Microbiology Veterinary College and Research InstituteUdumalpetTamil Nadu 642126India

Management strategies and the use of advanced technologies are equally important for determining the sample number and sampling frame for successful field sampling for animal disease prevalence *** quality of the biological samples collected in the field has a direct bearing on the integrity of the data generated,prevalence estimates and subsequent policy decisions on disease ***,compromising the quality of biological samples collected in the field could potentially undermine the priority setting principles in disease control *** samples collected from domestic animals in the field are precious materials and require meticulous planning for sample collection,sample storage in the field,transportation,and storage in the *** managed field sampling has a significant detrimental impact on the sample quality and quantity and directly affects the accuracy of disease prevalence data.A bad choice of sampling tools,containers,storage and transport all have a negative impact on the integrity of the sample and consequently have an impact on the *** the last two years,as part of our one health animal sampling work in ndia,we have observed challenges and opportunities in the field sampling of animals for disease prevalence *** paper aims to provide information on management practices and technologies for efficient biological sample collection from the field and ensure that good quality samples are available fortesting.

关键词： One health Domestic animals Sample quality Technology Management

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区结核病规程下化学治疗后涂片阳性肺结核病人的评价

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国际结核病与肺部疾病杂志 1995年第1期 16-22页

作者： Manjula Datta M.P.Radhamani R.selvaraj C.N.Paramasivan B.N.gopalan C.R.Sudeendra R.Prabhakar 屠德华 Tuberculois Research centre ICMRchetputMadrasIndia

这是1986年4月—1899年3月间北Arcot区开始抗结核化学治疗的3357名涂片阳性病人回顾性队列分析的情况报告。1988年11月至1989年6月(治疗开始后6到36个月)间对这些病人做一次家庭访问并获得其中76％病人情况(包括死亡)的资料。治疗方案... 详细信息

这是1986年4月—1899年3月间北Arcot区开始抗结核化学治疗的3357名涂片阳性病人回顾性队列分析的情况报告。1988年11月至1989年6月(治疗开始后6到36个月)间对这些病人做一次家庭访问并获得其中76％病人情况(包括死亡)的资料。治疗方案是由病人选择的。2306(69％)病人接受短程方案(SCC),1051(31％)病人接受标准化学治疗(非SCC),在SCC和非SCC方案中分别有43％和35％病人完成了他们80％以上的治疗。总死亡率为28％,其余病人中,31％有活动性疾病并排菌,其中培养阳性的65％耐鼻烟肼,12％耐利福平。4％耐鼻烟肼和利福平,19％耐异烟肼和链霉素。一个重要的发现是,即使用药量不足他们应治疗的50％病人中,56％为结核菌阴性。然而,不充分或不规则化学治疗的病人比充分化学治疗的病人死亡率在4倍以上,涂片阳性率约2倍。短程方案病人与标准方案病人之间没有比较。因为是病人选择他们的治疗而且各组是不可比的。

关键词：化学治疗标准方案规程总死亡率细菌学治疗方案病人死亡痰标本阳性率回顾性

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