检索结果-南通市图书馆

Transcriptional search to identify and assess reference genes for expression analysis in Solanum lycopersicum under stress and hormone treatment conditions

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2022年第11期21卷 3216-3229页

作者： DUAN Yao-ke HAN Rong SU Yan WANG Ai-ying LI Shuang SUN Hao gong Hai-jun Shaanxi Engineering Research Center for Vegetables/College of Horticulture Northwest A&F UniversityYangling 712100ShaanxiP.R.China Henan Key Laboratory of Ion-Beam Bioengineering College of Agricultural SciencesZhengzhou UniversityZhengzhou 450000HenanP.R.China

Tomato(Solanum lycopersicum)is a model plant for research on fruit development and stress response,in which gene expression analysis is frequently *** PCR(qPCR)is a widely used technique for gene expression analysis,and the selection of reference genes may affect the accuracy of results and even *** there have been some frequently used reference genes in tomato,it has been shown that the expressions of some of these genes are not constant in different tissues and environmental ***,little information on genomic identification of reference genes is available in ***,we mined the publicly available transcriptional sequencing data and screened out fifteen candidate reference genes,and the expression stability of these candidate genes and seven traditionally used ones were evaluated under stress and hormone *** results showed that over half of the selected candidate references were housekeeping genes in tomato *** the candidate reference genes and the traditionally used ones,the most stably expressed genes varied under different treatments,and most of these genes were recommended as preferred reference genes at least once except Solyc04g009030 and Solyc07g066610,two traditionally used reference *** study provides some novel reference genes in tomato,and the preferred reference genes under different environmental stimuli,which may be useful for future *** study suggests that excavating stably expressed genes from transcriptome sequencing data is a reliable approach to screening reference genes for qPCR analysis.

关键词： tomato(Solanum lycopersicum) gene expression qPCR reference gene expression stability

Estrogen reduces CCL_4-induced liver fibrosis in rats

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2002年第5期8卷 883-887页

作者： jun-Wang Xu jun gong Xin-Ming Chang Jin-Yan Luo Lei Dong Zhi-Ming Hao Ai Jia Gui-Ping Xu Department of Gastroenterology Second Hospital of Xi’an Jiaotong UniversityXi’an 710031Shaanxi ProvinceChina Department of Gastroenterology First Hospital of Xi’an Jiaotong UniversityXi’an 710061Shaanxi ProvinceChina

AIM: Chronic liver diseases, such as fibrosis or cirrhosis,are more common in men than in women. This genderdifference may be related to the effects of sex hormones onthe liver. The aim of the present work was to investigatethe effects of estrogen on CCL4-induced fibrosis of the liverin ***: Liver fibrosis was induced in male, female andovariectomized rats by CCL4 administration. All the groupswere treated with estradiol(1 mg/kg) twice weekly. Andtamoxifen wasgiven to male fibrosis model. At the end of 8weeks, all therats were killed to study serum indicators andthe ***: Estradiol treatment reduced aspartateaminotransferase(AST), alanine aminotransferase (ALT),hyaluronic acid(HA) and type IV collagen(CIV) in sera,suppressed hepatic collagen content, decreased the areas ofhepatic stellate cells (HSC) positive for α-smooth muscle actin(α-SMA), and lowered the synthesis of hepatic type I collagensignificantly in both sexes and ovariectomy fibrotic rats inducedby CCL4 administration. Whereas, tamoxifen had the oppositeeffect. The fibrotic response of the female liver to CCL4treatment was significantly weaker than that of male ***: Estradiol reduces CCL4-induced hepaticfibrosis in rats. The antifibrogenic role of estrogen in theliver may be one reason for the sex associated differencesin the progression from hepatic fibrosis to cirrhosis.

关键词：雌激素大鼠肝纤维化 CCL4 慢性肝病作用机制

Computer Vision-based Navigation and Predefined Track Following Control of a Small Robotic Airship

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

自动化学报 2007年第3期33卷 286-291页

作者： XIE Shao-Rong LUO jun RAO Jin-jun gong Zhen-Bang School of Mechatronics Engineering and Automation ShanghaiUniversity Shanghai 200072 P. R. China

为小机器的飞艇，飞艇应该能够跟随一条预定义的轨道，这被要求。在这份报纸，计算机为机器的飞艇的基于视觉的航行和最佳的模糊控制策略被建议。第一，视觉航行基于环境的自然里程碑被介绍。例如，当飞艇在一个城市上正在飞时，大楼能... 详细信息

为小机器的飞艇，飞艇应该能够跟随一条预定义的轨道，这被要求。在这份报纸，计算机为机器的飞艇的基于视觉的航行和最佳的模糊控制策略被建议。第一，视觉航行基于环境的自然里程碑被介绍。例如，当飞艇在一个城市上正在飞时，大楼能被用作其几何性质从数字地图或一个地理信息系统(GIS ) 被知道的视觉烽火。然后，几何方法论被采用关于飞艇的取向和位置提取信息。以便在一个预定义的磁道上保留飞艇，一个模糊飞行控制系统被设计，它把那些数据用作它的输入。并且基因算法(气体) ，一个通用全球优化方法，被利用优化模糊控制器的会员功能。最后，航行和控制策略被验证。

关键词：自动化系统飞行系统智能机器人宇宙飞船

Synthesis of Dendritic-Linear Block Copolymers by Atom Transfer Radical Polymerization

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2001年第7期12卷 595-596页

作者： You Liang ZHAO Ai jun gong Jing JIANG Hong Wei LIU Chuan Fu CHEN Fu XI State Key Laboratory of Polymer Physics and Chemistry Center of Molecular ScienceInstitute of ChemistryChinese Academy of SciencesBeijing 100080

The dendritic polyarylether 2-bromoisobutyrate as the macromolecular initiator for the controlled free radical polymerization of styrene was investigated. The polymerization was carried out with CuBr/2,2 ' -bipyridine catalyst at 110 degreesC. It is found that the hybrid dendritic-linear block copolymers possess well-defined molecular weights and low polydispersities.

关键词： Atom transfer radical polymerization dendrimer polystyrene block copolymer

Influence of education and working background on physicians' knowledge of secondary prevention guidelines for coronary heart disease: results from a survey in China

在线全文

学校读者我要写书评

暂无评论

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2012年第3期13卷 231-238页

作者： Yan-jun gong Tao HONG Jie JIANG Rong-hui YU Yan ZHANG Zhao-ping LIU Yong HUO Department of Cardiology Peking University First Hospital Beijing 100034 China

Background and objective： In clinical practice, the standard of secondary prevention for coronary heart disease （CHD） is quite disappointing in China. The physicians＇ shortage of knowledge of secondary prevention guidelines is thought to be a key factor contributing to the inadequate and delayed translation of guidelines into clinical practice. The purpose of this study is to investigate the influence of physicians＇ characteristics, including their educa- tion and work experience, on their knowledge of secondary prevention in China. Methods： A representative ques- tionnaire survey was made of physicians from cardiology departments in 35 tertiary hospitals in China. The survey contained 19 questions on knowledge of guideline recommendations for the secondary prevention of CHD. We col- lected basic information about the physicians, including their educational degree, clinical practice duration/work experience and geographic region. Results： In total, 864 physicians participated in the survey. Eight hundred and thirty-seven completed questionnaires were analyzed. For 6 of the 19 questions, physicians with a postgraduate degree were more likely to answer correctly than those without such a degree. For 11 of the 19 questions, physicians with more than three years＇ clinical experience were more likely to answer correctly than those who had less than three years＇ experience. For 5 of the 19 questions, *** eastern areas were more likely to answer correctly than those from mid/western areas. The mean total score of correct answers to the questionnaire was 11.69 points. Edu- cational degree and clinical practice duration affected total scores significantly while practice location did not （β=0.500, P=0.004; ,β=0.979, P=0.000; and 8=0.228, P=0.162, respectively）. Even if a relatively low score of 12 is taken as a threshold level of acceptable knowledge （defined as a pass）, the pass rate of all physicians was only 53.9%. Educational degree and clinical practice durat

关键词： Coronary heart disease Secondary prevention Guidelines Survey

Novel preparation of monolithic imprinted columns for electrochromatographic separation by photopolymerization

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2007年第6期18卷 734-737页

作者： Yu Ping Zhang Guo Qiang Zuo Wen jun gong Yue E.Deng Quan Ming Li Henan Institute of Science and Technology Xinxiang 453003China Department of Chemistry Henan Nomal UniversityXinxiang 453003China

A monolithic molecularly imprinted polymer with specific recognition ability for 4-hydroxybenzoic acid (4-HBA) was prepared by in situ photopolymerization, using methacrylic acid (MAA) as a functional monomer, ethylene glycol dimethacrylate (EDMA) as a cross-linking agent, toluene and isooctane as porogenic solvents and Irgacure 1800 as an initiator. Baseline separation of isomers of hydroxybenzoic acid was achieved in less than 8 min on this monolithic column using 4-HBA as template, but not on the blank polymer. Furthermore, some neutral compounds could also be baseline-separated on the imprinted polymer column in the mode of pressure-driven capillary electrochromatography.

关键词： Monolithic columns Molecularly imprinted polymer Electrochromatography Photopolymerization

Fluorinated p-n Type Copolyfluorene as Polymer Electret for Stable Nonvolatile Organic Transistor Memory Device

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Polymer Science 2016年第10期34卷 1183-1195页

作者： Bin Liu Yan Bao Hai-feng Ling Wen-sai Zhu Rui-jun gong Jin-yi Lin 解令海仪明东黄维 Centre for Molecular Systems and Organic Devices Key Laboratory for Organic Electronics and Information Displays & Institute of Advanced Materials Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM) Nanjing University of Posts & Telecommunications Nanjing 210023 China Key Laboratory of Flexible Electronics & Institute of Advanced Materials Jiangsu National Synergetic Innovation Center for Advanced Materials Nanjing Tech University Nanjing 211816 China

Abstract In this study, a kind of fluorinated copolyfluorene, named poly[（4-（octyloxy）-9,9-diphenylfluorene-2,7-diyl）-alt- （2,3,5,6-tetrafluoro-1,4-phenylene）] （PODPF-TFP）, is synthesized by facile palladium-based direct aromatization. Compared to the non-fluorinated counterpart, poly[（4-（octyloxy）-9,9-diphenylfluorene-2,7-diyl）-alt-（p-phenylene）] （PODPF-P）, deeper HOMO/LUMO energy level combined with steric hindrance effect endow PODPF-TFP with excellent spectra and morphology stability. Finally, organic field-effect transistor （OFET） memory devices are fabricated with PODPF-P/PODPF- TFP as the dielectric layers, and they both exhibit flash type storage characteristic. Owing to the electronegativity of fluorine atom, the device based on PODPF-TFP exhibits larger memory window and more stable Ion/Ioff ratio during a retention time of 10^4 s as well as a better aging stability. The present study suggests that fluorinated p-n copolyfluorene electrets could enhance the capabilities of charge trapping and storage, which are promising for OFET memory devices.

关键词： p-n copolyfluorenes Fluorine atom Direct aromatization Polymer dielectric Transistor memory.

Cloning,Tissue Distribution,and Transmembrane Orientation of the Olfactory Co-Receptor Orco from Two Important Lepidopteran Rice Pests,the Leaffolder(Cnaphalocrocis medinalis) and the Striped Stem Borer(Chilo suppressalis)

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2013年第10期12卷 1816-1825页

作者： LIU Su HUANG Yuan-jie QIAO Fei ZHOU Wen-wu gong Zhong-jun CHENG Jia-an ZHU Zeng-rong State Key Laboratory of Rice Biology/Key Laboratory of Agricultural Entomology Ministry of Agriculture/Institute of Insect Sciences Zhejiang University Institute of Plant Protection Henan Academy of Agricultural Sciences

In insects,the sense of smell is mainly mediated by olfactory receptors（Ors）.Olfactory co-receptor（Orco）,which is coexpressed with the Ors in almost all olfactory receptor neurons（ORNs）,is demonstrated to be an essential component in the insect olfactory *** can be potential target for developing novel olfactory-disruption strategy to control insect *** this study,two full-length cDNA sequences encoding Orcos（CmedOrco and ChsupOrco） were cloned from two Lepidopteran rice pests,the rice leaffolder,Cnaphalocrocis medinalis and the rice striped stem borer,Chilo *** amino acid sequences of CmedOrco and ChsupOrco showed high similarity to the previously identified Orcos from other insect species. Bioinformatic prediction and cellular immunofluorescence indicated that CmedOrco and ChsupOrco were both seven-transmembrane proteins with intracellular N-termini and extracellular *** expression levels of the two Orcos were much higher in male and female antennae than those in non-olfactory tissues,and the ChsupOrco transcripts reached a peak level in adults compared to other life *** results provide a foundation from which it will be possible to elucidate the roles of Orco in moth olfaction and for the development of environment-friendly management strategies of these two rice insect pests.

关键词： Cnaphalocrocis medinalis Chilo suppressalis olfactory co-receptor molecular cloning relative expression transmembrane orientation

Decreased expression of serotonin in the jejunum and increased numbers of mast cells in the terminal ileum in patients with irritable bowel syndrome

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2007年第45期13卷 6041-6047页

作者： Shen-Hao Wang Lei Dong Jin-Yan Luo jun gong Lu Li Xiao-Lan Lu Shui-Ping Han Department of Gastroenterology Second Hospital of Xi'an Jiaotong University Xi'an 710004 Shaanxi Province China Department of Pathology Medical School of Xi'an Jiaotong University Xi'an 710061 Shaanxi Province China

AIM: To investigate if there are changes in serotonin (5-HT) levels, enterochromaffin (EC) cells and mast cells in small intestinal mucosa of patients with irritable bowel syndrome (IBS). METHODS: Diarrhea-predominant (IBS-D, n = 20), or constipation-predominant (IBS-C, n = 18) IBS patients and healthy controls (n = 20) underwent colonoscopy and peroral small intestinal endoscopy, and mucosal samples were obtained at the descending part of the duodenum, proximal end of jejunum and terminal ileum. High-performance liquid chromatography- electrochemistry and immunohistochemical methods were used to detect 5-HT content, EC cells and mast cells. RESULTS: (1) There were no differences in the number and distribution of EC cells between IBS patients and the normal group. (2) The mucosal 5-HT contents at the duodenum, jejunum and ileum in IBS-C patients were 182 ± 90, 122 ± 54, 61 ± 35 ng/mg protein, respectively, which were all lower than those in the normal group (256 ± 84, 188 ± 91, and 93 ± 45 ng/ mg protein, respectively), with a significant difference at the jejunum (P < 0.05). There were no differences in the small intestinal mucosal 5-HT contents between IBS-D patients and the normal group. The mucosal 5-HT contents at the duodenum were significantly higher than those at the ileum in the three groups (P < 0.001). (3) The numbers of mast cells in patients with IBS-C and IBS-D at the ileum were 38.7 ± 9.4 and 35.8 ± 5.5/highpower field (hpf), respectively, which were significantly more than that in the normal group (29.8 ± 4.4/hpf) (P < 0.001). There was no significant difference in the numbers of mast cells at the other two parts between IBS patients and the normal group. The numbers of mast cells in IBS-C, IBS-D, and normal groups were all significantly higher at the ileum (38.7 ± 9.4, 35.8 ± 5.5, 29.8 ± 4.4/hpf, respectively) than at the duodenum (19.6 ± 4.7, 18.5 ± 6.3, 19.2 ± 3.3/hpf, respectively, P < 0.001). CONCLUSION: The changes in the 5-HT signaling pathway

关键词： Enterochromaffin cell Irritable bowelsyndrome Mast cell Serotonin Small intestinal mucosa