检索结果-南通市图书馆

Controlled WISP-1 shRNA Delivery Using Thermosensitive Biodegradable Hydrogel in the Treatment of Osteoarthritis

Journal of Bionic Engineering 2015年第2期12卷 285-293页

作者： gong yubao ma hecheng liu jianguo Department of Bone and Joint First Hospital of Jilin University Changchun 130021 China

This paper presents a new method of delivering shRNA with biodegradable, thermosensitive PLGA-PEG-PLGA hydrogels for gene treatment of osteoarthritis （OA）. OA is a chronic debilitating disease. Without the proper treatment and prognosis, it may result in the loss of joint function in aged people. Currently, gene therapy targeted on WISP-1 has emerged as an alternative method for OA treatment. In order to constantly release shRNA at 37.0 ℃, we synthetized the hydrogels via ring-opening copolymerization of lactide （LA） and glycolide （GA） using Polyethylene glycol （PEG Mn = 1000） and stannous octoate （Sn（Oct）2, 95%） as the macroinitiator and catalyst. First, the PLGA-PEG-PLGA copolymer was mixed with WISP- 1 shRNA and PEI-Lys in distilled water at 4.0 ℃. Then, the WISP-lshRNA/PEI-Lys loaded hydrogel was formed after incubation of the mixed solution at 37.0 ℃. During tests, the plasmid was released from this hydrogel complex constantly, and enhanced the transfection efficiency of WISP-1 shRNA. In addition, silencing WISP-1 results to lower expression of MMP-3 and ADAMTS, and the accumulation of HBP 1 in synoviocytes. Therefore, the hydrogel containing WISP-1 shRNA is demonstrated an efficient way for the treatment of OA.

关键词： osteoarthritis biodegradable thermosensitive hydrogel PLGA-PEG-PLGA WISP-1

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Rb-Sr and Sm-Nd Isochron Ages of the Dongmozhazhua and Mohailaheng Pb-Zn Ore Deposits in the Yushu area, southern Qinghai and Their Geological Implications

引用

Acta Geologica Sinica(English Edition) 2014年第2期88卷 558-569页

作者： TIAN Shihong gong Yingli YANG Zhusen HOU Zengqian liu Yingchao SONG Yucai XUE Wanwen LU Haifeng WANG Fuchun ZHANG yubao ZHU Tian YU Changjie School of Earth Science and Mineral Resources China University of Geosciences Key Laboratory of Metallogeny and Mineral Assessment Ministry of Land and ResourcesInstitute of Mineral ResourcesChinese Academy of Geological Sciences Laboratory of Department of Thermal Engineering Tsinghua University Institute of Geology Chinese Academy of Geological Sciences Qinghai Institute of geological Survey

Located on the northeast margin of the Qiangtang terrane between the Jinshajiang suture zone and Bangonghu-Nujiang suture zone,the Dongmozhazhua and Mohailaheng Pb-Zn deposits in the Yushu area of Qinghai Province are representative Pb-Zn deposits of the Pb-Zn-Cu polymetallic mineralization belt in the northern part of the Nujiang-Lancangjiang-Jinshajiang area,which are in the front belt of the Yushu thrust nappe *** formed environments of these two deposits are different from those of sediment-hosted base metal deposits elsewhere in the *** authors hold that they were formed during the Indian-Asian continental collision and developed within the fold-thrust belt combined with thrust and strike-slip-related Cenozoic basins in the interior of the collisional *** on the metallogenic epochs of these two deposits is helpful to the understanding of ore-forming regularity of the regional Pb-Zn-Cu mineralization belt and also to the search for new deposits in this *** age of the Dongmozhazhua deposit has been determined by the Rb-Sr isochron method for sphalerite residues,whereas the age of the Mohailaheng deposit has been determined by the Rb-Sr isochron method for sphalerite residues and the Sm-Nd isochron method for *** age of the Dongmozhazhua deposit is 35.0-±0.0 ma （（87Sr/86Sr）0=0.708807） for sphalerite *** age of the Mohailaheng deposit is 32.2±0.4 ma （（87Sr/86Sr）o=0.708514） for sphalerite residues and 31.8±0.3 ma （（143Nd/144Nd）o=0.512362） for fluorite with an average of 32.0 *** with the regional geological setting during mineralization,a possible tectonic model for metallogeny of the Dongmozhazhua and Mohailaheng Pb-Zn deposits has been *** two ages are close to the ages of the Pb-Zn deposits in the Lanping and Tuotuohe basins,indicating that it is possible that the narrow 1000-kilometer-long belt controlled by a thrust nappe system on the eastern and northern margins of the Tibetan plateau could

关键词： Rb-Sr and Sm-Nd isochron ages thrust nappe system tectonic model Dongmozhazhua and Mohailaheng Pb-Zn deposits in the Yushu area Qinghai Province

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：