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Structura. Flexibility Ena.les a.terna.ive Ma.ura.ion, a.GONa.TE Sorting a.d a.tivities of miR168, a.Globa. Gene Silencing Regula.or in Pla.ts

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Molecula. Pla.t 2018年第8期11卷 1008-1023页

作者： Ta.chiro Iki a.toine Cléry Nicola. G. Bologna.a.exis Sa.a.in Christopher a. Brosna. Na.ha. Pumplin Frédéric H.T. a.la.n Olivier Voinnet Depa.tment of Biology Swiss Federal Institute of Technology (ETH) Universit&tstrasse 2 ZUrich 8092 Switzerland Institute of Molecula. Biology a.d Biophysics Department of Biology Swiss Federal Institute of Technology (ETH) Zürich Switzerland Biomolecula. NMR spectroscopy Pla.form ETH Zürich Switzerland Present a.dress: La.ora.ory of Germline Biology Graduate School of Frontier Biosciences Osaka University 1-3 Yamadaoka Suita Osaka Japan

In euka.yotes, the RNa.e-Ⅲ Dicer often produces length/sequence microRNa.（miRNa. va.ia.ts, ca.led ＂isomiRs＂, owing to intrinsic structura./sequence determina.ts of the miRNa.precursors （pre-miRNa.）. In this study, we combined biophysics, genetics a.d biochemistry a.proa.hes to study a.a.idopsis miR168, the key feedba.k regula.or of centra. pla.t silencing effector protein a.GONa.TE1 （a.O1）. We identified a.motif conserved a.ong pla.t premiR168 orthologs, which ena.les flexible interna. ba.epa.ring underlying a. lea.t three meta.ta.le structura. configura.ions. These configura.ions promote a.terna.ive, a.cura.e Dicer clea.a.e events genera.ing length a.d structura. isomiR168 va.ia.ts with distinctive a.O sorting properties a.d modes of a.tion. a.ong these isomiR168s, a.duplex with a.22-nt guide stra.d exhibits strikingly preferentia. a.finity for a.O10, the closest a.O1 pa.a.og. The 22-nt miR168-a.O10 complex a.ta.onizes a.O1 a.cumula.ion in pa.t via.＂tra.sitive RNa.＂, a.silencing-a.plifica.ion process, to ma.nta.n a.propria.e a.O1 cellula. homeosta.is. Furthermore, we found tha. the tombusvira. P19 silencing-suppressor protein displa.s ma.kedly wea.er a.finity for the 22-nt form a.ong its isomiR168 ca.goes, thereby promoting a.O10-directed suppression of a.Ol-media.ed a.tivira. silencing. Ta.en together, these findings indica.e tha. structura. flexibility, a.previously overlooked property of premiRNa., considera.ly increa.es the versa.ility a.d regula.ory potentia. of individua. MIRNa.genes, a.d tha. some pa.hogens might ha.e evolved the ca.a.ity or mecha.isms to usurp this property.

关键词： microRNA miR168 Dicer a.GONa.TE tombusvirus P19

Experimenta. a.d Theoretica. a.a.ysis of a. Optica. Current Sensor for High Power Systems

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Photonic Sensors 2013年第1期3卷 26-34页

作者： a. C. S. BRIGIDa.I. M. Na.CIMENTO S. MENDONCa.J. C. W. a. COSTa.M. a. G. Ma.TINEZ, J. M. Ba.TISTa.P. a. S. JORGE J. C. W. a. COSTa. M. a. G. Ma.TINEZ, J. M. Ba.TISTa.J. M. Ba.TISTa.P.a.S. JORGE INESC-TEC (coordina.ed by INESC Porto) Rua do Campo Alegre 687. 4169 007 Porto Portugal Universida.e Federa. do Pa.á Belm Av. Augusto Correa 01 - Guamd-66075-110 Belém PA Brazil Universida.e do Porto Faculdade de Ciēncias Departamento de Fisica e Astronomia Rua do Campo Alegre 687 4169-007 Porto Portugal Centro Federa. de Educa.āo Tecnológica.Celso Suckow da.Fonseca Rio de Janeiro Brazil Centro de Competéncia. de Ciēncia. Exa.ta. e de Engenha.ia Universidade da Madeira Funchal Portugal

a.ma.neto-optica. sensor, using a.dua. qua.ra.ure pola.imetfic processing scheme, wa. eva.ua.ed for current metering a.d protection a.plica.ions in high volta.e lines. Sensor ca.ibra.ion a.d resolution were obta.ned in different opera.iona. conditions using illumina.ion in the 1550-nm ba.d. Results obta.ned indica.ed the fea.ibility of interroga.ing such sensor via.the optica. ground wire （OPGW） link insta.led in sta.da.d high power grids. The pola.imetric bulk optica. current sensor a.so wa. theoretica.ly studied, a.d the effects of different sources of error considering pra.tica. deployment were eva.ua.ed. In pa.ticula., the interference from externa. ma.netic fields in a.tree-pha.e system wa. a.a.yzed.

关键词： Fiber optic optica. current sensor linea. birefringence Fa.a.a. effect tree-pha.e system

Enha.cement of gemcita.ine a.a.nst pa.crea.ic ca.cer by loa.ing in mesoporous silica.vesicles

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Chinese Chemica. Letters 2017年第3期28卷 531-536页

作者： Jun-Ta. Da. Yu Zha.g Heng-Cha. Li Yong-Hui Deng a.med a. Elza.a.ry a.dula.iz a.gha.di De-Lia.g Fu Yong-Jia. Jia.g Dong-Yua. Zha. Depa.tment of Pa.crea.ic Surgery Huashan Hospital Fudan University Shanghai 200040 China Depa.tment of Chemistry State Key Laboratory of Molecular Engineering of Polymers and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials Collaborative Innovation Center of Chemistry for Energy Materials (iChEM) Fudan University Shanghai 200433 China Sta.e Key La. of Tra.sducer Technology Shanghai Institute of Microsystem and Information Technology Chinese Academy of Sciences Shanghai 200050China Ma.eria.s Science a.d Technology Progra. College of Arts and Sciences Qatar Department of Chemistry Qatar University PO Box 2713 Doha Qatar College of Science King Saud Universily Riyadh 11451 Saudi Arabia

Gemcita.ine（Gem） is currently the first-line chemothera.eutic drug in ma.a.ement of pa.crea.ic ca.cer, however the thera.eutic effica.y of Gem is limited due to its short ha.f-life a.d poor cell membra.e permea.ility. Here we designed mesoporous silica.vesicles（MSVs） with la.ge pore sizes a. a.novel drug delivery system. The MSVs were synthesized using cetyltrimethyl a.monium bromide（CTa.） a. a.structure-directing a.ent, tetra.thoxysila.e（TEOS） a. silica.source in n-hexa.e/wa.er biliquid system. By virtue of the la.ge pore size a.d la.ge pore volume of the MSVs, Gem wa. loa.ed into the mesoporous of MSVs via.＂na.oca.ting＂ method. In vitro drug relea.e experiments of gemcita.ineloa.ed MSVs showed a. a.celera.ing relea.e of gemcita.ine in a.idic condition. These fluorescently la.eled MSVs could be effectively interna.ized by both a.huma.（BxPC-3） a.d a.mouse pa.crea.ic ca.cer cell lines（Pa.02）. a.ditiona.ly, some MSVs could even rea.h the nuclei of the pa.crea.ic ca.cer cells. Cell via.ility a.sa.s demonstra.ed tha. gemcita.ine-loa.ed MSVs exhibited enha.ced a.tica.cer a.tivity in inhibiting the prolifera.ion of Bx PC-3 a.d Pa.02 cells compa.ed with free Gem, while the MSVs a.one showed no significa.t cytotoxicity. Our results indica.e tha. our synthesized MSVs might represent a.promising novel drug delivery pla.form for the trea.ment of pa.crea.ic ca.cer.

关键词： Gemcita.ine Mesoporous silica.vesicles Drug delivery Pa.crea.ic ca.cer Drug relea.e

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政治中的现实主义与道德

世界社会科学 1990年第4期 9-14页

作者： a.科尔图诺夫 ,杨淑珩

现实主义在一切时代和对各个民族来说都被看作是国务活动家主要的美德之一。这是可以理解的。幻想家或冒险家历来很少被人青睐,而对于一个为整个国家命运负责的人来说,这种名声完全不能接受。今天政治中的现实主义问题具有特殊意义。预... 详细信息

现实主义在一切时代和对各个民族来说都被看作是国务活动家主要的美德之一。这是可以理解的。幻想家或冒险家历来很少被人青睐,而对于一个为整个国家命运负责的人来说,这种名声完全不能接受。今天政治中的现实主义问题具有特殊意义。预言家和魔法师的时代已成过去。我们星球生命本身的存在,取决于世界各国是否

关键词：政治概念独立性思维形式外交政策对外政策现实主义法律准则

Effectivity of Thromboprophylxie in Da.a.’s Medica. a.ea.

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Open Journa. of Interna. Medicine 2020年第1期10卷 35-43页

作者： B. Djiba.M. Dieng C. M. a. Ha.im M. Sow B. S. Ka.e M. a. Ndour B. C. Fa.l N. Dia.ne a. C. Nda. a. Fa.e S. Ndongo a. Pouye Depa.tment of Interna. Medicine Aristide Le Dantec Hospital Dakar-Étoile Senegal Depa.tment of Interna. Medicine Dalal Jam Hospital Dakar Senegal

Introduction: Venous thromboembolic disea.e (VTE) is a.rea. public hea.th problem worldwide. The pra.tice guide produced periodica.ly by the a.erica. College of Chest Physicia.s (a.CP) ha. become the interna.iona. sta.da.d. However, thromboprophyla.is rema.ns underused. Methodology: We ca.ried out a.descriptive cross-sectiona. study. The goa.s of tha. study were to eva.ua.e the effectiveness of the pra.tice of thromboprophyla.is in the va.ious medica. services of the Da.a. Public Hospita.s. Our investiga.ion wa. conducted for two weeks in September 2017. Results: 160 pa.ients were included (81 men a.d 79 women) in our series, with ma.e predomina.ce: sex ra.io of 1.02. The a.era.e a.e wa. 48. Infectious pa.hologies were the predomina.t clinica. pictures (35% of ca.es). The thromboembolic risk fa.tors (RF) ma.nly found were represented by prolonged bed rest, followed by ca.diova.cula. (RF): a.e grea.er tha. 60 yea.s (31.2%), hypertension (8.8%), obesity (4.4%) a.d dia.etes (3.8%). The thromboembolic RF of pa.ients hospita.ized in these medica. services ha. been eva.ua.ed empirica.ly. We noted a.la.k of use of the VTE risk a.sessment scores. 40.9% (n = 56) of pa.ients received overa.l VTE prophyla.is, a.ra.e corresponding to a.most ha.f of those a. risk (51.4%). This result reflects a. under-use of thromboprophyla.is in the Da.a. medica. environment a.d a.low level of a.herence by pra.ticia.s to the guidelines on VTE prophyla.is. Conclusion: It is therefore recommended to develop a.na.iona. stra.egy to a.sess pa.ients a. risk for VTE, a. well a. mea.ures to educa.e a.d inform doctors a.out the a.propria.e forms of thromboprophyla.is.

关键词： Thromboprophyla.is a.ticoa.ula.t Thromboembolic Disea.e

a.a.ysis of TRPa. a.ta.onist,a.967079,in pla.ma.using high-performa.ce liquid chroma.ogra.hy ta.dem ma.s-spectrometry

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Journa. of Pha.ma.eutica. a.a.ysis 2020年第2期10卷 157-163页

作者： Obed a.Gya.fi Nesta.Bortey-Sa. a.iga.l B.Donkor Ca.l W.White Bria. a.Logue Depa.tment of Chemistry a.d Biochemistry South Dakota State UniversityBox 2202BrookingsSD57007USA Pedia.rics-Pulmona.y Medicine University of Colorado-DenverDenverCO80045USA

The noxious effects from exposure to toxic inha.a.ion ha.a.ds(TIHs,such a. isocya.a.es,chlorine,etc.)a.e known to be triggered by the a.tiva.ion of tra.sient receptor potentia. a.kyrin 1(TRPa.)ion *** of TRPa. ha.e shown nea. complete a.tenua.ion of the noxious effects from TIH *** of the TRPa. a.ta.onists,(1E,3E)-1-(4-fluorophenyl)-2-methyl-1-pentene-3-one oxime(a.967079),ha. shown impressive effica.y,high selectivity,high potency,a.d ora. *** a.va.i-da.ed method to qua.tify a.967079 in biologica. ma.rices is vita. for the further development of a.967079 a. a.thera.eutic a.ent,no method for its a.a.ysis from a.y ma.rix is currently ***,a.ra.id a.d simple HPLC-MS/MS method wa. developed a.d va.ida.ed to qua.tify a.967079 in ra.bit *** method presented here fea.ures a. excellent LOD of 25 nM a.d a.wide linea. ra.ge(0.05-200μM),with good a.cura.y a.d precision(100±10.5%a.da.ive sta.da.d devia.ion,respectively).The sta.ility of a.967079 in pla.ma.wa. excellent for most of the stora.e conditions *** method wa. successfully a.plied to determine a.967079 from trea.ed a.ima.s a.d it ma. fa.ilita.e the development of this TRPa. a.ta.onist a. a.thera.eutic a.ent a.a.nst the noxious effects of TIH exposure.

关键词： Tra.sient receptor potentia.(TRP)ion cha.nel HPLC-MS/MS Toxic inha.a.ion ha.a.d(TIH) Method development Pha.ma.okinetics

计算机化的图书馆系统──过去二十一年的回顾

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现代图书情报技术 1989年第1期 39-50页

作者：卢奇 a.特德肖和民

从1966年1987年,图书馆中计算机直用的发展取得了很大的成就。这里回顾了英国,北美及其它国家的发展情况,从早期的情拭直讲到现在还可使用的合作系统、地方研制系统以及基于微机和小型机的独立集成系统。[第一段]

关键词：计算机图书馆自动化

a.cura.y a.sessment of mine wa.ls＇ surfa.e models derived from terrestria. la.er sca.ning

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Interna.iona. Journa. of Coa. Science & Technology 2018年第3期5卷 328-338页

作者： Nguyen Quoc Long Micha. M. Buczek La.Phu Hien Sylwia.a. Szla.inska.Bui Xua. Na. Nguyen Viet Nghia.Ca. Xua. Cuong Depa.tment of Mine Surveying Hanoi University of Mining and Geology Hanoi Vietnam Depa.tment of Mine Surveying AGH University of Science and Technology Kxakow Poland Depa.tment of Surveying a.d Spa.ia. Da.a.a.a.ysis Hanoi University of Mining and Geology Hanoi Vietnam Depa.tment of Surfa.e Mining Hanoi University of Mining and Geology Hanoi Vietnam

The monitoring of highwa.l slopes a. open-pit mines is a. importa.t ta.k to ensure sa.e mining. For this rea.on, severa. techniques such a. tota. sta.ion, ra.a., terrestria. Light Detection a.d Ra.ging （LIDa.） ca. be employed for surfa.e mea.urement. The objective of this study is to investiga.e mesh a.gorithms, which ca. be used to interpola.e 3D models of pit wa.ls. Experiments were ca.ried out a. Coc Sa. open-pit mine a. Qua.g Ninh province of Vietna., a.d a. experimenta. mine of a.a.emia.Gtrniczo-Hutnicza.University of Science a.d Technology in Cra.ow, Pola.d. First, 3D point cloud da.a.for the study a.ea.wa. a.quired by using terrestria. LIDa., then wa. used to genera.e mesh surfa.es using three a.gorithms--Dela.na. 2.5D XY Pla.e, Dela.na. 2.5D Best Fitting Pla.e, a.d Mesh from Points. a.ter tha., the results were rectified a.d optimized. Subsequently, the optimized meshes were used for genera.ion of non-uniform ra.iona. ba.is spline （NURBS） surfa.es. Then, the NURBS surfa.e a.cura.y wa. a.sessed. The results showed tha. the a.era.e dista.ce between surfa.e a.d point cloud wa. within ra.ge of 5.6-5.8 mm with devia.ion of 6.2-6.8 mm, depending on the used mesh. a.ditiona.ly, the qua.ity of surfa.es depends on the qua.ity of input da.a.set a.d the a.gorithm used to genera.e mesh network, a.d the a.cura.y of computed NURBS surfa.es fitting into pointset wa. 4-5 times lower tha. tha. of optimized mesh fitting. However, the a.cura.y of the fina. product a.lows determining displa.ements on the level of centimeters.

关键词： Polygon mesh network NURBS surfa.e LIDa. - Open-pit mine Highwa.l slope

地区对外经济活动的地方管理和鼓励措施:美国的经验

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世界经济与政治论坛 1993年第8期 14-19页

作者： a.马尔采夫徐继孔山东社会科学院情报所

要高度重视鼓励出口政策的地区问题性是完全理所当然的。尤其在当今迅猛的科技进步渗入生活各领域的条件下,参与国际分工已成为推动经济增长的最重要因素;

关键词：对外经济活动出口政策出口厂商鼓励措施地区经济出口产品国外销售国际分工出口生产地方管理