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Binding of Mono, Bi and Tridomain Proteins to Zwitterionic and Anionic Vesicles： Asymmetric Location of Anionic Phospholipids in Mixed Zwitterionic/Anionic Vesicles and Cooperative Binding

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Journal of Life Sciences 2011年第3期5卷 167-181页

作者： Francisco Torrens gloria Castellano Institute for Molecular Science University of Valencia Valencia E-46071 Spain Energesis Chair of Interdisciplinary Technology Saint Vincent Martyr Catholic University of Valencia Valencia E-46003 Spain

Lysozyme, myoglobin and BSA were used as models of globular proteins covering a wide range of pl. The purpose is to extend the studies to anionic lipid bilayers. Electrostatics is studied in cationic protein adsorption to zwitterionic PC and anionic mixed PC/PG SUVs. Protein adsorption is investigated in SUVs along with changes of fluorescence emission spectra. Partition coefficients and cooperativity parameters are calculated. At pl binding obtains maximum while at lower or higher pHs binding decreases. In Gouy-Chapman formalism activity coefficient goes with square charge, which deviations indicate asymmetric location of anionic phospholipid in the inner leaflet, in mixed SUVs for lysozyme- and myoglobin-PC/PG systems, in agreement with experiments and molecular dynamics simulations. Vesicles bind myoglobin anti-cooperatively while lysozyme-BSA cooperativitivey. A model is proposed for both, which composes two protein sub-layers with different structures and properties. Hill coefficient reflects subunit cooperativity of bi and tridomain proteins.

关键词： Protein-lipid interaction sigmoid adsorption isotherm transbilayer asymmetry anionic phospholipid asymmetric location cooperative binding hill plot.

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香港的生物多样性及其保育工作

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生物多样性 2000年第1期8卷 25-35页

作者：刘惠宁侯智恒萧丽萍周锦超香港新界大埔林锦路嘉道里农场暨植物园香港新界大埔林锦路嘉道里农场暨植物园

香港位于热带，属海洋性气候。地势崎岖多山，山地约占全港总面积的3/4。城市发展多集中在沿海平坦地带。目前香港的城市和乡镇面积约占总面积的20%，农地约占5%(当中大部份已遭荒废)，余下的均为郊野地区，这包括天然林和人工林(约占1... 详细信息

香港位于热带，属海洋性气候。地势崎岖多山，山地约占全港总面积的3/4。城市发展多集中在沿海平坦地带。目前香港的城市和乡镇面积约占总面积的20%，农地约占5%(当中大部份已遭荒废)，余下的均为郊野地区，这包括天然林和人工林(约占14%)、灌丛(约占36%)及草地(约占17%)。由于良好的气候和地理条件，形成了众多不同的生态环境，使总面积仅1090 km2的弹丸之地孕育出种类多样的动植物，生物多样性十分丰富。香港约有2500种原生植物，包括被子植物约1900种，裸子植物7种，蕨类植物220多种及苔藓植物300多种。动物方面，已记录的野生哺乳类动物有40多种，鸟类超过459种，两栖类23种，爬行类70多种。昆虫种类繁多，其中蜻蜓目100多种，鳞翅目2200多种(蝴蝶200多种，蛾类2000多种)。有很多是国家保护物种和特有种。植物方面属国家一级保护的有1种——刺桫椤(Alsophila spinulosa)；国家二级保护的有6种，如四药门花(Tetrathryrium subcordatum)；国家三级保护的有8种，如穗花杉(Amentotaxus argotaenia)。此外，香港特有种有16种，例如紫萁科(Osmundaceae)的粤紫萁(Osmunda mildei)、马兜铃科(Aristolochiaceae)的香港细辛(Asarum hongkongense)和兰科(Orchidaceae)的谢氏卷瓣兰(Bulbophyllum

关键词：香港生物多样性保育工作环境保育

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Total esophagogastrectomy plus extended lymphadenectomy with transverse colon interposition:A treatment for extensive esophagogastric junction cancer

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World Journal of Hepatology 2015年第22期7卷 2411-2417页

作者： Marco Ceroni Enrique Norero Juan Pablo Henríquez Eduardo Vinuela Eduardo Briceno Cristian Martínez gloria Aguayo Fernando Araos Paulina González Alfonso Díaz Mario Caracci Department of Digestive Surgery Pontificia Universidad Católica de Chile School of MedicineHospital Sótero del Rio Pathological Anatomy Department Hospital Sótero del Rio Radiology Department Hospital Sótero del Rio Research Nurse Hospital Sótero del Rio

AIM:To review the post-operative morbidity and mortality of total esophagogastrectomy(TEG) with second barrier lymphadenectomy(D2) with interposition of a transverse colon and to determine the oncological outcomes of TEG D2 with interposition of a transverse ***:This study consisted of a retrospective review of patients with a cancer diagnosis who underwent TEG between 1997 and 2013. Demographic data,surgeryprotocols,complications according to Clavien-Dindo classifications,final pathological reports,oncological follow-ups and causes of death were recorded. We used the TNM 2010 and Japanese classifications for nodal dissection of gastric cancer. We used descriptive statistical analysis and Kaplan-Meier survival curves. A P-value of less than 0.05 was considered statistically ***:The series consisted of 21 patients(80.9% men). The median age was 60 years. The 2 main surgical indications were extensive esophagogastric junction cancers(85.7%) and double cancers(14.2%). The mean total surgery time was 405 min(352-465 min). Interposition of a transverse colon through the posterior mediastinum was used for replacement in all cases. Splenectomy was required in 13 patients(61.9%),distal pancreatectomy was required in 2 patients(9.5%) and resection of the left adrenal gland was required in 1 patient(4.7%). No residual cancer surgery was achieved in 75.1% of patients. A total of 71.4% of patients had a postoperative complication. Respiratory complications were the most frequently observed complication. Postoperative mortality was 5.8%. Median follow-up was 13.4 mo. Surgery specific survival at 5 years of follow-up was 32.8%; for patients with curative surgery,it was 39.5% at 5 ***:TEG for cancer with interposition of a transverse colon is a very complex surgery,and it presents high post-operative morbidity and adequate oncological outcomes.

关键词： Esophagogastric junction cancer Total esophagogast

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Overexpression Of Protein P16ink4a As A Marker Of Squamous Intraepithelial Lesions Induced By High Risk Hpv Infection In Mexican Women

Overexpression Of Protein P16ink4a As A Marker Of Squamous I...

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24th International Papillomavirus Conference and Clinical Workshop

作者： Luz del Carmen Alarcon-Romero Elba Reyes-Maldonado Berenice Illades-Aguiar Eugenia Flores-Alfaro Amafia Vences Velazquez gloria Fernondez-Tilapa Esther Ivonne Lopez-Bayghen Ethel A Garcia-Latorre Patricia Romero Neri Victor Gabriel Hernandez Chavez Marco Antonio teron-Porcayo Escuela Nacional de Ciencias Biologicas IPNMexicoD.F.Facultad de Ciencias Quimico BiologicasUniversidad Autonoma de GuerreroChilpancingo.GuerreroMexico Escuela Nacional de Ciencias Biologicas IPNMexicoD.F Facultad de Ciencias Quimico Biologicas Universidad Autonoma de GuerreroChilpancingo.GuerreroMexico Facultad de Ciencias Quimico Biologicas Universidad Autonoma de GuerreroChilpancingo.GuerreroMexico Escuela Nacional de Ciencias Biologicas IPNMexicoD.F.Facultad de Ciencias Quimico BiologicasUniversidad Autonoma de GuerreroChilpancingo.GuerreroMexico Escuela Nacional de Ciencias Biologicas IPNMexicoD.F.Facultad de Ciencias Quimico BiologicasUniversidad Autonoma de GuerreroChilpancingo.GuerreroMexico Depatamento de Genetica y Biologia Molecular CIVESTAV-IPNMexicoD.F Escuela Nacional de Ciencias Biologicas IPNMexicoD.F Clinica de Displasias ChilpancingoGuerreroMexico Departamento de Anatomia patologica Hospital de Cardiologia Centro Medico Nacional Siglo XXI del IMSSMexicoD.F Instituto Estatal de Cancerologia‘Dr Arturo Beltran Ortega’ AcapulcoGuerreroMexico

＜正＞Background:Expression of the high-risk human papillomavirus（hr-hpv）e7-gene replicating epithelial cells results in disruption of the prb-e2f complex and degradation of prb This leads to strong overexpression o...

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