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An Approach to Quantify Endomembrane Dynamics in Pollen Utilizing Bioactive Chemicals

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Molecular Plant 2013年第4期6卷 1202-1213页

作者： Nolan Ung Michelle Q. Brown glenn R. Hicks Natasha V. Raikhel Center for Plant Cell Biology and Department of Botany and Plant sciences University of California Riverside Riverside CA 92521 USA

Tip growth of pollen tubes and root hairs occurs via rapid polar growth. These rapidly elongating cells require tip-focused endomembrane trafficking for the deposition and recycling of proteins, membranes, and cell wall materials. Most of the image-based data published to date are subjective and non-quantified. Quantitative and com- parative descriptors of these highly dynamic processes have been a major challenge, but are highly desirable for genetic and chemical genomics approaches to dissect this biological network. To address this problem, we screened for small molecules that perturbed the localization of a marker for the Golgi Ras-like monomeric G-protein RAB2：GFP expressed in transgenic tobacco pollen. semi-automated high-throughput imaging and image analysis resulted in the identifica- tion of novel compounds that altered pollen tube development and endomembrane trafficking. six compounds that caused mislocalization and varying degrees of altered movement of RAB2：GFP-labeled endomembrane bodies were used to generate a training set of image data from which to quantify vesicle dynamics. The area, velocity, straightness, and intensity of each body were quantified using semi-automated image analysis tools revealing quantitative differences in the phenotype caused by each compound. A score was then given to each compound enabling quantitative comparisons between compounds. Our results demonstrate that image analysis can be used to quantitatively evaluate dynamic sub- cellular endomembrane phenotypes induced by bioactive chemicals, mutations, or other perturbing agents as part of a strategy to quantitatively dissect the endomembrane network.

关键词： endomembranes vesicle trafficking quantification pollen chemical biology.

six high-intensity interval training sessions over 5 days increases maximal oxygen uptake,endurance capacity,and sub-maximal exercise fat oxidation as much as 6 high-intensity interval training sessions over 2 weeks

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Journal of sport and Health science 2021年第4期10卷 478-487页

作者： Muhammed M.Atakan Yasemin Guzel suleyman Bulut sukran N.Kosar glenn K.McConell Huseyin H.Turnagol Division of Nutrition and Metabolism in Exercise Faculty of Sport SciencesHacettepe UniversityAnkara 06690Turkey Institute for Health and sport Victoria UniversityMelbourneVIA 3011Australia

Background:High-intensity interval training(HIIT)induces similar or even superior adaptations compared to continuous endurance ***,just 6 HIIT sessions over 2 weeks significantly improves maximal oxygen uptake(VO_(2max)),submaximal exercise fat oxidation,and endurance *** even faster adaptations can be achieved with HIIT is not ***,we aimed to determine whether 2 sessions of HIIT per day,separated by 3 h,every other day for 5 days(double HIIT(HIIT-D),n=15)could increase VO_(2max),submaximal exercise fat oxidation,and endurance capacity as effectively as 6 sessions of HIIT over 2 weeks(single HIIT(HIIT-s),n=13).Methods:Each training session consisted of 10×60 s of cycling at 100%of VO_(2max)interspersed with 75 s of low-intensity cycling at 60 watt(W).Pre-and post-training assessments included VO_(2max),time to exhaustion at-80%of VO_(2max),and 60-min cycling trials at-67%of VO_(2max).Results:similar increases(ps:6.0%,p>0.05)and endurance capacity(HIIT-D:80.1%***-s:79.2%,p>0.05)were *** exercise carbohydrate oxidation was reduced in the 2 groups after exercise training(HIIT-D:9.2%,p=0.014 ***-s:18.8%,p=0.012)while submaximal exercise fat oxidation was significantly increased in HIIT-D(15.5%,p=0.048)but not in HIIT-s(9.3%,p=0.290).Conclusion:six HIIT sessions over 5 days was as effective in increasing VO_(2max)and endurance capacity and was more effective in improving submaximal exercise fat oxidation than 6 HIIT sessions over 2 weeks.

关键词： Double interval training Exercise metabolism Performance substrate oxidation

Accuracy and reliability of feature selection by Chinese fingerprint examiners

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Forensic sciences Research 2017年第4期2卷 203-209页

作者： shiquan Liu Zhongliang Mi glenn MLangenburg Jian Wu Institute of Evidence Law and Forensic science China University of Political Science and LawBeijingChina shanghai Key Laboratory of Crime scene Evidence ShanghaiChina Minnesota Bureau of Criminal Apprehension St.PaulMNUSA College of sciences Northeastern UniversityShenyangChina

The selection of minutiae is a critical part of the analysis phase within the fingerprint identification methodology,known as *** study assessed the accuracy and reliability of the minutiae selections of 92 Chinese fingerprint examiners during the analysis phase,absent an exemplar print,of the fingerprint identification ***,we measured the accuracy(trueness)of their annotation of minutiae,and we measured their reliability which is the reproducibility and repeatability in their annotations in one complex mark by using R *** observed significant variation within inter-and intra-examiner annotations of the *** saw no statistically significant differences for the variability of minutiae annotations based on the participant’s sex or years of experience.

关键词： Forensic science fingerprint ACE-V minutiae reliability reproducibility feature selection

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Dimorphism in Fungi

Dimorphism in Fungi

structural development of vegetation on rehabilitated North stradbroke Island:Above/belowground feedback may facilitate alternative ecological outcomes

2007中华医学会第二次医学真菌学术会议暨医学真菌实验室研究技术新进展学习班

作者： glenn s.Bulmer Peking Medical University and Xinjiang Peoples’Hospital

Many of the etiologic agents of systemic mycoses exits in two different *** is the saprophytic phase,which lives in nature and multiplies with hyphae and spores:this is the infectious *** second form is the so-called pathogenic form which develops in a host from the infectious form. These two forms differ in morphology,chemistry,antigenicity,habitat,and serologic response,ie.,one may exist in a hyphae/spore form while the other is a pure yeast. These differences must be understood if we are to successfully diagnose and treat systemic mycoses.

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Ecological Processes 2013年第1期2卷 195-211页

作者： Patrick Audet Amanda Gravina Vanessa glenn Phill McKenna Helen Vickers Melina Gillespie David Mulligan Centre for Mined Land Rehabilitation Sustainable Minerals Institute The University of QueenslandBrisbane(St.Lucia)QLD 4072Australia

Introduction:This study depicts broad-scale revegetation patterns following sand mining on North stradbroke Island,south-eastern Queensland,***:Based on an ecological timeline spanning 4–20 years post-rehabilitation,the structure of these ecosystems(n=146)was assessed by distinguishing between periods of‘older’(pre-1995)and‘younger’(post-1995)rehabilitation ***:The general rehabilitation outlook appeared promising,whereby an adequate forest composition and suitable levels of native biodiversity(consisting of mixed-eucalypt communities)were achieved across the majority of rehabilitated sites over a relatively short ***,older sites(n=36)appeared to deviate relative to natural analogues as indicated by their lack of under-storey heath and simplified canopy composition now characterised by mono-dominant black sheoak(Allocasuarina littoralis)reaching up to 60%of the total tree *** changes coincided with lower soil fertility parameters(e.g.,total carbon,total nitrogen,and nutrient holding capacity)leading us to believe that altered growth conditions associated with the initial mining disturbance could have facilitated an opportunistic colonisation by this *** established,it is suspected that the black sheoak’s above/belowground ecological behaviour(i.e.,relating to its leaf-litter allelopathy and potential for soil-nitrogen fixation)further exacerbated its mono-dominant distribution by inhibiting the development of other native ***:Although rehabilitation techniques on-site have undergone refinements to improve site management,our findings support that putative changes in edaphic conditions in combination with the competitive characteristics of some plant species can facilitate conditions leading to alternative ecological outcomes among rehabilitated *** on these outcomes,future studies would benefit from in depth spatio-temporal analyses to verify these mechanisms at finer investigative sc

关键词： Eucalypts Mono-dominance Forest regeneration Ecological restoration Mineral sands Mining

Calibration after bootstrap for accurate uncertainty quantification in regression models

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npj Computational Materials 2022年第1期8卷 1092-1100页

作者： glenn Palmer siqi Du Alexander Politowicz Joshua Paul Emory Xiyu Yang Anupraas Gautam Grishma Gupta Zhelong Li Ryan Jacobs Dane Morgan Department of Computer sciences University of Wisconsin-MadisonMadisonWIUSA Department of Materials science and Engineering University of Wisconsin-MadisonMadisonWIUSA

Obtaining accurate estimates of machine learning model uncertainties on newly predicted data is essential for understanding the accuracy of the model and whether its predictions can be trusted.A common approach to such uncertainty quantification is to estimate the variance from an ensemble of models,which are often generated by the generally applicable bootstrap *** this work,we demonstrate that the direct bootstrap ensemble standard deviation is not an accurate estimate of uncertainty but that it can be simply calibrated to dramatically improve its *** demonstrate the effectiveness of this calibration method for both synthetic data and numerous physical datasets from the field of Materials science and *** approach is motivated by applications in physical and biological science but is quite general and should be applicable for uncertainty quantification in a wide range of machine learning regression models.

关键词： ensemble applicable uncertainty

Comparing carbon accumulation in restored and natural wetland soils of coastal Louisiana

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International Journal of sediment Research 2019年第6期34卷 600-607页

作者： glenn M.suir Charles E.sasser Ronald D.DeLaune Elizabeth O.Murray U.s.Army Engineer Research and Development Center Environmental LaboratoryWetlands and Environmental Technologies Research Facility Louisiana state University Department of Oceanography and Coastal ScienceCollege of the Coast and Environment U.s.Army Engineer Research and Development Center Environmental Laboratory

Louisiana’s chronic wetland deterioration has resulted in massive soil organic matter loss and subsequent carbon release through *** combat these losses,and reestablish ecosystem function,goods,and services,many restoration projects have been constructed or planned throughout coastal *** are significant data gaps and conflicting results regarding wetland contributions to global warming,especially related to carbon sequestration in restored *** exceptionally large data set was used to derive carbon accumulation rates from key soil characteristics and *** and comparisons of bulk density,organic matter,total carbon,vertical accretion(short-and longer-term),and carbon accumulation rates were made across time(chronosequence) and space(i.e.,coastwide,watershed basins,and vegetation zones).Carbon accumulation rates in the Louisiana coastal zone were generally correlated to hydrogeomorphology,with higher rates occurring in zones of high river connectivity or in swamp or higher salinity tolerant *** average,naturally occurring wetlands had higher carbon accumulation rates than restoration *** some restoration measures were higher,and most showed increasing carbon accumulation rates over *** demonstrate that although wetland restoration provides many ecosystem benefits,the associated carbon sequestration may also provide useful measures for climate change management.

关键词： Wetland productivity Chronosequence Carbon sequestration Wetland restoration Coastal Louisiana