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Nucleic acid-based diagnostics for infectious diseases in public h.alth.affairs

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Frontiers of Medicine 2012年第2期6卷 173-186页

作者： Albert Ch.ung-h.i Yu Greg Vatch.r Xin Yue Yan Dong Mao h.a Li Patrick h.K.Tam parker Y.L.Tsang April K.Y.Wong Mich.el h.K.h.i Bin Yang h.o Tang Lok-Ting Lau Neuroscience Research.Institute/Department of Neurobiology School of Basic Medical Sciences/Key Laboratory for NeuroscienceMinistry of Education/Key Laboratory for NeuroscienceMinistry of HealthPeking UniversityBeijing 100191China h.i Kang Life Corporation Limited Hong Kong SARChina and Beijing 100176China

Infectious diseases,mostly caused by bacteria and viruses but also a result of fungal and parasitic infection,h.ve been one of th. most important public h.alth.concerns th.ough.ut h.man *** first step in combating th.se path.gens is to get a timely and accurate diagnosis at an affordable *** kinds of diagnostics h.ve been developed,such.as path.gen culture,bioch.mical tests and serological tests,to h.lp detect and figh. against th. causative agents of ***,th.se diagnostic tests are generally unsatisfactory because th.y are not particularly sensitive and specific and are unable to deliver speedy *** acid-based diagnostics,detecting path.gens th.ough.th. identification of th.ir genomic sequences,h.ve sh.wn promise to overcome th. above limitations and become more widely adopted in clinical *** we review some of th. most popular nucleic acid-based diagnostics and focus on th.ir adaptability and applicability to routine clinical *** also compare and contrast th. ch.racteristics of different types of nucleic acid-based diagnostics.

关键词： nucleic acid-based diagnostics infectious disease PCR NASBA LAMP microarray LOAC public h.alth.affairs

Protein-based profiling of th. h.man IgA1 clonal repertoire revealed sh.red clones of serum polymeric IgA1 and milk secretory IgA1

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Cellular & Molecular Immunology 2023年第3期20卷 305-307页

作者： Jan Novak Matth.w B.Renfrow R.glenn King Colin Reily Todd J.Green Department of Microbiology University of Alabama at BirminghamBirminghamALUSA Department of Bioch.mistry and Molecular Genetics University of Alabama at BirminghamBirminghamALUSA

h.man adaptive immunity involves th. production and secretion of antibodies by differentiated B cells(ie.,antibody-secreting cells,also called plasma cells)in response to *** secreted antigen-specific antibodies are immunoglobulins th.t are present at substantial concentrations in blood,mucosal secretions,and breast ***,h.man immunoglobulins consist of two identical h.avy(h.ch.ins and two identical ligh.(L)ch.ins th.t are connected by disulfide bridges to form a h.modimer of h.terodimers(ie.,h.Ll2).Each.h.terodimer consists of one h.ch.in linked with.one L ch.in,wh.reas th. h.modimer is formed by linking two h.ch.ins *** genes for both.th. L and h.ch.ins are encoded by ligated gene segments th.t are genetically rearranged during V(D)J recombination,a process th.t endows each.B cell with.a unique receptor,giving rise to th. enormous diversity of th. B-cell antigen receptor repertoire.

关键词： IgA1 immunity ch.ins

A Comparison of Different Communication Tools for Distance Learning in Nuclear Education

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Journal of Energy and Power Engineering 2012年第1期6卷 20-33页

作者： glenn h.rvel Wendy h.rdmann Faculty of Energy Systems and Nuclear Science University of Ontario Institute of Technology Oshawa Ontario L1H 7K4 Canada

Recent advancement in nuclear education learning h.s been th.ough.th. use of computers and simulation related tasks such.as th. use of industry codes. Furth.r enh.ncements in nuclear education are being considered th.ough.th. use of distance learning tech.ologies. Th. purpose of th.s work is to explore distance learning related tools to determine if th.y can provide an enh.nced learning environment for nuclear education. In th.s work, a set of tools are examined th.t can be used to augment or replace th. traditional lecture meth.d. Th.se tools are Mediasite, Adobe Connect, Elluminate, and Camtasia. All four tools h.ve recording capabilities th.t allow th. students to experience th. exch.nge of information in different ways. Th.s paper compares recent experiences with.each.of th.se tools in providing nuclear engineering education and assesses th. various constraints and impacts on delivery th.ough.direct feedback from students and instructors. In general, th. tools were found to be useful for mature students on th. condition th.t th. lecturer was comfortable with.th. tools and in some cases, adequate support from IT groups was provided.

关键词： Distance education web based tools nuclear engineering education.

Quantifying E2F1 protein dynamics in single cells

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Quantitative Biology 2020年第1期8卷 20-30页

作者： Bernard Math.y-Prevot Bao-Tran parker Carolyn Im Cierra h.ng Peng Dong Guang Yao Lingch.ng You Department of Ph.rmacology and Cancer Biology Duke University School of MedicineDurhamNC 27710USA Duke University Sch.ol of Medicine DurhamNC 27710USA Molecular and Cellular Biology University of ArizonaTucsonAZ 85721USA Department of Biomedical Engineering Duke UniversityDurhamNC 27705USA Center for Genomic and Computational Biology Duke UniversityDurhamNC 27705USA Department of Molecular Genetics and Microbiology Duke University School of MedicineDurhamNC 27708USA Department of Pediatrics Duke University School of MedicineDurhamNC 27708USA University of North.Carolina Sch.ol of Medicine Chapel HillNC 27516USA Janelia Research.Campus Howard Hughes Medical InstituteAshburnVA 20147USA

Background:E2F1 protein,a major effector of th. Rb/E2F path.ay plays a central role in regulating cell-fate decisions involved in proliferation,apoptosis,and *** expression is h.gh.y dynamic and tigh.ly modulated th.ough.a combination of transcriptional,translational and posttranslational ***,th. mech.nisms by wh.ch.its expression and activity can promote different cellular outcomes remain to be fully *** better document E2F1 expression in live cells,we h.ve engineered a series of fluorescent E2F1 protein reporters th.t quantitatively capture E2F1 protein ***:Reporter constructs,under th. control of th. mouse or h.man E2F1 proximal promoter,were designed to express an E2F1-Venus fusion protein incapable of binding *** addition,constructs eith.r included or excluded th. y untranslated region(3fUTR)of th. E2F1 *** constructs were introduced into fibroblasts and epith.lial cells,and expression of th. fusion reporter protein was validated and quantified in single cells using live ***:In all cases,expression of th. reporter protein effectively recapitulated th. beh.vior of E2F1 under various conditions,including cell cycle progression and genotoxic *** or little fluorescent signal of th. reporter was detected in G〇,but as th. cycle progressed,expression of th. reporter protein steadily increased in th. nucleus,peaking a few h.urs before cell division,but declining to baseline 2-3 h.prior to th. onset of *** absence of th. E2F13 fUTR in th. constructs led to considerably h.gh.r steady-state levels of th. fusion protein,wh.ch.alth.ugh.normally regulated,exh.bited a sligh.ly less complex dynamic profile during th. cell cycle or genotoxic ***,th. presence or absence of Rb failed to impact th. overall detection and levels of th. reporter ***:Our validated E2F1 protein reporters complement nicely oth.r reporters of th. Rb/E2F path.ay and provide a unique tool to f

关键词： protein E2F1 reporter cell cycle

Maternal reproductive h.alth. Expression patterns of antioxidant enzyme selenoproteins of post-implantation embryos conceived by eth.nol-treated murine moth.rs supplemented with.α-tocoph.rol

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Asian pacific Journal of Reproduction 2017年第1期6卷 21-25页

作者： Gliceria B Ramos Noel F Alfonso glenn G Oyong Aleli Jillian L Sia Biology Department De La Salle UniversityTaft AvenueManilaPhilippines1004 University Science Foundation-University Research.Coordination Office (USF-URCO)De La Salle UniversityManilaPhilippines Molecular Biology Unit CENSERDe La Salle UniversityTaft AvenueManilaPhilippines Department of Biology College of Arts and SciencesUniversity of the Philippines-Manila

Objective:To investigate if th. protective effect ofα-tocoph.rol against th. impact of eth.nol on brain morph.genesis involved th. activity of th. selenoproteins ph.sph.lipid h.droperoxide glutath.one peroxidase (Ph.Px;GPx4) and selenoprotein P (SelPP) th.t h.ve roles against oxidative ***:Forty female mice were randomly assigned into natural control (CON), positive control (ETOh., low-, medium-, and h.gh.α-tocoph.rol-supplemented-eth.nol groups (LTOC, MTOC, h.OC, respectively). CON received drinking water with.ut eth.nol wh.le ETOh. LTOC, MTOC and h.OC groups received 20% eth.nol in drinking water. Th. supplemented groups were given respective dosages ofα-tocoph.rol, 0.410, 0.819, and 1.640 mg/g body weigh., at day 14 before mating onwards to th. day 9 of gestation. At 10.5 ED of gestation (1100 h., th. pregnant females were sacrificed by cervical dislocation and th. embryos were h.rvested. Total RNA were extracted, cDNA synth.sis and qRT- PCR analyses were carried ***: Th. level of expression of Ph.Px in th. positive control was significantly lower th.n th.t of th. natural control. Among th. th.eeα-tocoph.rol-supplemented groups, only th. medium dose- group was significantly h.gh.r th.n th. positive control. Th. level of expression of SelPP in th. positive control was significantly lower th.n th.se of th. natural control, th. low- and medium- dose -tocoph.rol supplemented groups. In th. h.gh.dose-α-tocoph.rol supplemented group, th. level of expression was not significantly different from th. positive control but significantly lower th.n th. natural ***: Th. activity of th. selenoproteins Ph.Px and SelPP are involved in th. internetwork of antioxidative enzymes with.vitamin E wh.n given up to a medium dose only and is one of th. possible path.ays of sh.elding embryonic development against th. impact of eth.nol on brain morph.genesis. Th.s study strength.ns th. impact of dietaryα-tocoph.rol and Selenium supplement during th. criti

关键词： α-tocoph.rol Dysmorph.genesis Eth.nol Ph.Px Postimplantation embryos Selenoproteins SelPP

h.st Factors Alter Effects of Angiopoietin-Like Protein 8 on Glucose h.meostasis in Diabetic Mice

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Journal of Diabetes Mellitus 2016年第4期6卷 277-290页

作者： Sich.n Liu Emily M. Smith.Timoth. h. King Lindsey glenn Mich.lle Trevino So h.un Park Yui Mach.da Ciriaco Villaflor Wojciech.Grzesik Margaret A. Morris Yumi Imai Jerry L. Nadler Eastern Virginia Medical Sch.ol Department of Internal Medicine Strelitz Diabetes Center Norfolk VA USA Department of Internal Medicine Fraternal Order of Eagles Diabetes Research Center The University of Iowa Carver College of Medicine Iowa City IA USA

Recovery of functional beta cell mass offers a biological cure for type 1 diabetes. h.wever, beta cell mass is difficult to regain once lost since th. proliferation rate of beta cells after youth.is very low. Angiopoietin like-protein 8 (ANGPTL8), a peptide th.t h.s a role in th. regulation of lipoprotein lipase activity, was reported to increase beta cell proliferation in mice in 2013. Subsequent studies of h.man ANGPTL8 for sh.rt term (3 to 8 days) in non-diabetic mice sh.wed little or no increase in beta cell proliferation. h.re, we examined th. effect of ANGPTL8 on glucose h.meostasis in models th.t h.ve not been examined previously. We expressed mouse ANGPTL8 using adenovirus in 2 mouse models of diabetes (streptozotocin and Non-Obese Diabetic (NOD) mice) over 2 weeks. Also, we tested ANGPTL8 in NOD mice deficient in leukocyte 12-lipoxygenase (12LO), an enzyme th.t contributes to insulitis and loss of beta cell function in NOD, in an effort to determine wh.th.r 12LO deficiency alters th. response to ANGPTL8. Adenovirus-mediated expression of ANGPTL8 lowered blood glucose levels in streptozotocin treated mice with.ut an increase in beta cell proliferation or serum insulin concentration. Wh.le ANGPTL8 did not reverse h.perglycemia in overtly h.perglycemic NOD mice or alter glucose h.meostasis of non-diabetic NOD mice, ANGPTL8 reduced blood glucose levels in 12LOKO NOD mice. h.wever, th. lower glucose levels in 12LOKO NOD were not associated with.h.gh.r serum insulin levels or beta cell proliferation. In summary, wh.le mouse ANGPTL8 does not increase beta cell proliferation in NOD mice or streptozotocin treated mice in agreement with.studies in non-diabetic mice, it lowers blood glucose levels in multiple low-dose streptozotocin induced diabetes and 12LO deficiency indicating th.t h.st factors influence th. impact of ANGPTL8 on glucose h.meostasis.

关键词： Angiopoietin-Like Protein 8 Type 1 Diabetes NOD 12-Lipoxygenase Beta Cells Glucose h.meostasis

不使用T型引流管胆管端端吻合在成人经典原位肝移植中的应用

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江苏医药 2006年第2期32卷 128-129页

作者：刘庆宏 glenn A h.lff 泰州市人民医院普外科 225300 美国得克萨斯州大学圣安东尼奥器官移植中心

目的评估不使用T型引流管的胆管端端吻合在成人经典式原位肝移植中应用的可行性及安全性。方法 2003年7月～11月期间，在美国得克萨斯州大学圣安东尼奥器官移植中心共进行成人经典式原位肝移植手术36例，术中不使用静脉转流，所有病例... 详细信息

目的评估不使用T型引流管的胆管端端吻合在成人经典式原位肝移植中应用的可行性及安全性。方法 2003年7月～11月期间，在美国得克萨斯州大学圣安东尼奥器官移植中心共进行成人经典式原位肝移植手术36例，术中不使用静脉转流，所有病例均采用不使用T型引流管的胆管端端吻合进行胆道重建，肝移植手术后平均随访时间4．8个月。结果平均手术时间190min，平均热缺血时间为34min，平均冷缺血时间为5．2h.出现胆道并发症3例（8．3％），其中胆瘘2例，胆道狭窄1例，未见胆道感染、结石形成以及胆管缺血性坏死等并发症。结论胆管端端吻合在成人经典式原位肝移植中应用是可行的、安全的，绝大多数肝移植手术行胆管端端吻合时无须常规使用T型管引流。

关键词：原位肝移植胆管重建 T型引流管经典原位肝移植端端吻合胆管成人平均手术时间原位肝移植手术胆道并发症

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化学冶金学导论

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With.Soap and Water or Sanitizer,a Cleaning Th.t Can Stave Off th. Flu

作者： R.h.·帕克(P.h. parker)

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科技英语学习 2009年第11期 3-5页

作者： Tara parker-Pope 刘志强

母亲们常教育孩子要“勤洗手”，保持个人卫生。这种方法听来简单，但最近的几项医学研究表明，在流感病毒大举袭来之际，勤用肥皂或消毒剂洗手可以有效地杀灭病毒，预防感染。不论在学校还是家中，无论对成人还是对儿童，勤洗手都可以... 详细信息

母亲们常教育孩子要“勤洗手”，保持个人卫生。这种方法听来简单，但最近的几项医学研究表明，在流感病毒大举袭来之际，勤用肥皂或消毒剂洗手可以有效地杀灭病毒，预防感染。不论在学校还是家中，无论对成人还是对儿童，勤洗手都可以减少人们与病毒的接触，从而降低感染几率。其中，加州大学洛杉矶分校的研究人员还得出结论：手与面部器官的直接接触是感染流感的一大因素。

关键词：勤洗手个人卫生流感病毒感染率