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Ex vivo ovine model for teaching open laryngotracheal surgery

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World Journal of Otorhinolaryngology-Head and Neck Surgery 2018年第2期4卷 140-144页

作者： Ahmed M.S. Soliman David c. Ianacone glenn c. Isaacson Department of Otolaryngology — Head & Neck Surgery Lewis Katz School of Medicine Temple University Philadelphia PA 19140 USA Department of Pediatrics Lewis Katz School of Medicine Temple University Philadelphia PA 19140 USA

Objective: To develop an animal model for teaching open laryngotracheal surgical procedures. Methods: The heads and necks from 5 pre-pubescent sheep were harvested after humane anesthesia. After 2—5 days to allow for rigor mortis to resolve, a specimen was supported with sandbags on an operating table. Operative procedures including tracheotomy, medialization laryngoplasty, anterior cartilage grafting, tracheal resection with primary anastomosis, and laryngectomy with closure of the pharynx were attempted. Results: The ovine head and neck provided an accurate model for simulation of all attempted procedures. Ovine tissue resembled that of humans in mechanical properties and handling. Postsurgical endoscopy confirmed graft alignment. conclusions: The sheep head and neck provides an inexpensive, realistic, and safe model for surgical training for a variety of open laryngotracheal procedures. This is particularly relevant given the recent emphasis on surgical simulation and the relative rarity of some of these pro-cedures in residency training.

关键词： Airway surgery Laryngotracheal reconstruction Sheep Surgical simulation Tracheal resection

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Intracellular Delivery of mRNA in Adherent and Suspension cells by Vapor Nanobubble Photoporation

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Nano-Micro Letters 2020年第12期12卷 404-420页

作者： Laurens Raes Stephan Stremersch Juan cFraire Toon Brans glenn Goetgeluk Stijn De Munter Lien Van Hoecke Rein Verbeke Jelter Van Hoeck Ranhua Xiong Xavier Saelens Bart Vandekerckhove Stefaan De Smedt Koen Raemdonck Kevin Braeckmans Laboratory of General Biochemistry&Physical Pharmacy Ghent University9000 GhentBelgium cancer Research Institute Ghent(cRIG) 9000 GhentBelgium Department of Diagnostic Sciences Ghent University9000 GhentBelgium VIB-UGent center for Medical Biotechnology 9052 GhentBelgium Department of Biomedical Molecular Biology Ghent University9000 GhentBelgium Department of Biochemistry and Microbiology Ghent University9000 GhentBelgium

Efficient and safe cell engineering by transfection of nucleic acids remains one of the long-standing hurdles for fundamental biomedical research and many new therapeutic applications,such as cAR T cell-based *** has recently gained increasing attention as a more safe and versatile alternative tool over viral-or DNA transposon-based approaches for the generation of adoptive T ***,limitations associated with existing nonviral mRNA delivery approaches hamper progress on genetic engineering of these hard-to-transfect immune *** this study,we demonstrate that gold nanoparticle-mediated vapor nanobubble(VNB)photoporation is a promising upcoming physical transfection method capable of delivering mRNA in both adherent and suspension *** transfection experiments on HeLa cells showed the importance of transfection buffer and cargo concentration,while the technology was furthermore shown to be effective for mRNA delivery in Jurkat T cells with transfection efficiencies up to 45%.Importantly,compared to electroporation,which is the reference technology for nonviral transfection of T cells,a fivefold increase in the number of transfected viable Jurkat T cells was ***,our results point toward the use of VNB photoporation as a more gentle and efficient technology for intracellular mRNA delivery in adherent and suspension cells,with promising potential for the future engineering of cells in therapeutic and fundamental research applications.

关键词： Transfection mRNA Photoporation Optoporation Gold nanoparticles Vapor nanobubbles

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博物馆和可持续发展的在地文化

美术馆 2020年第2期 8-9页

作者： glenn c.Sutter

自从人类世界在近二百年从全新世(Holocene)进入人类世(Anthropocene),全球化背景下人类对自然和生态的干预愈加深入,以至于人类集体活动所带来的影响不仅限于自然资源,而是更加不可避免地对在地文化造成了威胁。有学者提出,相比于自然...

自从人类世界在近二百年从全新世(Holocene)进入人类世(Anthropocene),全球化背景下人类对自然和生态的干预愈加深入,以至于人类集体活动所带来的影响不仅限于自然资源,而是更加不可避免地对在地文化造成了威胁。有学者提出,相比于自然,保护在地文化或许更应该成为"可持续发展"的核心概念。博物馆在保护在地文化的可持续发展上扮演着重要的角色,但目前的博物馆还有很多可改进的空间。对于在地博物馆来说,

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cannabinoids function in defense against chewing herbivores in cannabis sativa L.

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Horticulture Research 2023年第11期10卷 131-140页

作者： George M.Stack Stephen I.Snyder Jacob A.Toth Michael A.Quade Jamie L.crawford John K.McKay John Nicholas Jackowetz Ping Wang glenn Philippe Julie L.Hansen Virginia M.Moore Jocelyn K.c.Rose Lawrence B.Smart Horticulture Section School of Integrative Plant ScienceCornell UniversityCornell AgriTechGenevaNY 14456United States Plant Biology Section School of Integrative Plant ScienceCornell UniversityIthacaNY 14853United States Plant Breeding Section School of Integrative Plant ScienceCornell UniversityIthacaNY 14853United States Department of Agricultural Biology Colorado State UniversityFort CollinsCO 80523United States cirona Labs GenevaNY 14456United States Department of Entomology Cornell UniversityCornell AgriTechGenevaNY 14456United States

In the decades since the first cannabinoids were identified by scientists,research has focused almost exclusively on the function and capacity of cannabinoids asmedicines and intoxicants for humans and other *** little is knownabout the adaptive value of cannabinoid production,though several hypotheses have been proposed including protection from ultraviolet radiation,pathogens,and *** test the prediction that genotypeswith greater concentrations of cannabinoidswill have reduced herbivory,a segregating F2 population of cannabis sativa was leveraged to conduct lab-and field-based bioassays investigating the function of cannabinoids in mediating interactions with chewing *** the field,foliar cannabinoid concentration was inversely correlated with chewing herbivore *** detached leaves,Trichoplusia ni larvae consumed less leaf area and grew less when feeding on leaves with greater concentrations of *** electron and light microscopy were used to characterize variation in glandular trichome ***-free genotypes had trichomes that appeared *** isolate cannabinoids from confounding factors,artificial insect diet was amended with cannabinoids in a range of physiologically relevant *** grew less and had lower rates of survival as cannabinoid concentration *** results support the hypothesis that cannabinoids function in defense against chewing herbivores.

关键词： function founding ultraviolet

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The Role of Phenolic Antioxidants in Lubricant Protection and Deposit control

作者： (加)亚历山大

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The Role of Phenolic Antioxidants in Lubricant Protection an...

中国润滑技术论坛（2018）暨中国汽车工程学会汽车燃料与润滑油分会第十八届年会

作者： glenn V.Kenreck Tom Tang SI Group Inc.

The performance of antioxidants is critical for protection of the lubricant from degradation that could be detrimental to service life of lubricants and the lubricated *** two types of antioxidants typically used in lubricant additive formulations are alkylated diphenylamines and alkylated *** reactions of these antioxidants are *** bench performance testing of these two types of antioxidants are *** data analysis using contour mapping of the performance tests demonstrates the effectiveness of a high-level formulation design to find an optimum antioxidant ratio for an Alkylated Phenol with an alkylated *** optimums for the specific formulation and oil that was used in the test are *** presentation provides an understanding of the performance of both phenolic and aminic antioxidants as well as the interactions of these two types in lubricant formulations.

关键词： aminic antioxidant phenolic antioxidant lubricant formulation antioxidant PDSc TEOST 33c deposit control ETHANOX 4716

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如何限制暂态恢复电压

吉林电力 1982年第6期 72-77页

作者： glenn c.Vietor William E.Harper 陈政

工业制造部门一直关注增加输配电断路器的遮断能力,然而为了安全运行,断路器的性能还必须符合另外一些具有同等重要性的指标:满载电流、系统过电压以及暂态恢复电压(TRY)。人们有重视故障电流的增加而忽视TRV问题的倾向,但是TRV对已设... 详细信息

工业制造部门一直关注增加输配电断路器的遮断能力,然而为了安全运行,断路器的性能还必须符合另外一些具有同等重要性的指标:满载电流、系统过电压以及暂态恢复电压(TRY)。人们有重视故障电流的增加而忽视TRV问题的倾向,但是TRV对已设计制造、并按照ANSI标准经过检验的断路器,同样会引起破坏性的后果。断路器承受TRV的能力,根据ANSI标准来确定。利用这些标准,对一台给定的断路器,可以绘制出相应于额定电流和故障电流水平的额定恢复电压性能曲线。额定恢复

关键词：暂态恢复电压上升速率 ANSI TRV 断路器限制

High-efficiency reflector-less dual-level silicon photonic grating coupler

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光子学研究:英文版 2023年第7期11卷 1275-1283页

作者： VALERIO VITALI THALíA DOMíNGUEZ BUcIO cOSIMO LAcAVA RIccARDO MARcHETTI LORENZO MASTRONAR TEERAPAT RUTIRAWUT glenn cHURcHILL JOAQUíN FANEcA JAMES c.GATES FREDERIc GARDES PERIKLIS PETROPOULOS Optoelectronics Research centre University of SouthamptonSouthamptonSO171BJUK Electrical Computer and Biomedical Engineering DepartmentUniversity of Pavia27100 PaviaItaly Advanced Fiber Resources Milan S.r.l. 20098 San Donato MilaneseItaly currently at Instituto de Microelectrónica de Barcelona IMB-CNM(CSIC)Campus UAB08193 BellaterraSpain

We present the design and experimentally demonstrate a dual-level grating coupler with subdecibel efficiency for a 220 nm thick silicon photonics waveguide which was fabricated starting from a 340 nm silicon-on-insulator *** proposed device consists of two grating levels designed with two different linear apodizations,with opposite chirping signs,and whose period is varied for each scattering unit.A coupling efficiency of-0.8 d B at1550 nm is experimentally demonstrated,which represents the highest efficiency ever reported in the telecommunications c-band in a single-layer silicon grating structure without the use of any backreflector or indexmatching material between the fiber and the grating.

关键词： waveguide scattering grating

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2001年未来展望 4