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Efficacy assessment of peracetic acid in the removal of synthetic 17α-ethinyl estradiol contraceptive hormone in wastewater

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Journal of Environmental Sciences 2020年第3期32卷 1-8页

作者： Rita Maurício Flávia Semedo Rita Dias Joao P.Noronha Leonor amaral Michiel A.Daam Antonio P.Mano Mário S.Diniz CENSE Center for Environmental and Sustainability ResearchNOVA School of Science and TechnologyNOVA University LisbonQuinta da Torre2829-516 CaparicaPortugal Department of Environmental Sciences and Engineering NOVA School of Science and TechnologyNOVA University LisbonQuinta da Torre2829-516 CaparicaPortugal REQUIMTE/FCT NOVA School of Science and TechnologyNOVA University LisbonQuinta da Torre2829-516 CaparicaPortugal Biotox Lab UCIBIODepartment of ChemistryNOVA School of Science and TechnologyNOVA University LisbonQuinta da Torre2829-516 CaparicaPortugal

Increasing concerns have been raised on endocrine disrupting chemicals like the sex hormone 17 a-ethinylestradiol(EE2),the more since traditional wastewater(WW) treatments appear to be ineffective for their *** efficacy of the relatively novel disinfectant peracetic acid(PAA) in EE2 removal was evaluated,as well as its potential effects on WW quality *** treatments tested for EE2 removal were also evaluated in terms of toxicity,through the determination of biochemical responses(antioxidant enzymes,lipid peroxidation and vitellogenin induction) using zebrafish(Danio rerio) as a biological *** contact times less than 20 min appeared insufficient regardless of the PAA dose tested,but a 100% EE2 removal was attained at a PAA concentration of 15 mg/L with a contact time of20 *** suspended solids,chemical oxygen demand and pH in PAA treatments remained well within levels set in European legislation for WW ***2 induced signific ant increased vitellogenin(VTG) levels in both female and male fish,indic ating increased estrogenic activity,especially in males suggesting an endocrine disruption *** the addition of PAA(15 mg/L),however,VTG levels in both sexes returned to control *** this PAA treatment showed increased levels of the antioxidant enzyme catalase,the lipid peroxidation levels were similar or even lower than in *** the results suggest that the use of PAA appears a promising way forward as a less toxic alternative to chlorine disinfection with high efficiency in the removal of EDC like EE2.

关键词： EE2 Wastewater treatment Zebrafish Biomarkers

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The role of rhizobacteria in rice plants: Growth and mitigation of toxicity

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Journal of Integrative Agriculture 2018年第12期17卷 2636-2647页

作者： Marcela C F Rêgo Aline F Cardoso Thayná da C Ferreira Marta C C de Filippi Telma F V Batista Rafael G Viana gisele B da Silva Plant Protection Laboratory Institute of Agrarian Sciences Federal Rural University of Amazon Phytopathology Laboratory Brazilian Enterprise for Agricultural Research-Rice and Beans

Allelopathic compounds reduce the growth and productivity of upland rice plants, especially in consecutive plantations. The rhizobacteria Pseudomonas fluorescens BRM-32111 and Burkholderia pyrrocinia BRM-32113 have been recorded as growth promoters in rice. This study was developed to understand the effect of the application of rhizobacteria on upland rice plants in consecutive plantations. Experiments were conducted in a completely randomized design with four replications of four treatments: rice seed inoculated with P. fluorescens BRM-32111, rice seed inoculated with B. pyrrocinia BRM-32113(both sown on soil with rice residue), non-inoculated plants sown on soil with rice residue(control with residue(WR)), and non-inoculated plants on soil with no residue(NR). Roots and seedling growth were adversely affected by allelopathic compounds in control WR plants. Plants inoculated with rhizobacteria P. fluorescens BRM-32111 or B. pyrrocinia BRM-32113 induced an increase of 88% in biomass, 3% in the leaf area, 40% in length, 67% in root biomass, 21% in chlorophyll a, 53% in chlorophyll(a+b), 50% in rate of carbon assimilation(A), 227% in A/rubisco carboxylation efficiency(Ci) and 63% in water use efficiency(WUE) compared to control WR plants. These results indicate that rhizobacteria P. fluorescens BRM-32111 and B. pyrrocinia BRM-32113 increase the tolerance of rice plants to stress from allelochemicals. There are possible practical agricultural applications of these results for mitigating the effects of environmental allelochemistry on upland rice.

关键词： allelopathy B.pyrrocinia P.fluorescens rhizobacteria rice

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Endoscopic ultrasound staging for early esophageal cancer: Are we denying patients neoadjuvant chemo-radiation?

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World Journal of Gastroenterology 2017年第46期23卷 8193-8199页

作者： Carrie Luu Marisa amaral Jason Klapman Cynthia Harris Khaldoun Almhanna Sarah Hoffe Jessica Frakes Jose M Pimiento Jacques P Fontaine Department of Thoracic Oncology H. Lee Moffitt Cancer Center and Research Institute Department of Gastrointestinal Oncology H. Lee Moffitt Cancer Center and Research Institute Department of Radiation Oncology H. Lee Moffitt Cancer Center and Research Institute

AIM To evaluate the accuracy of endoscopic ultrasound(EUS) in early esophageal cancer(EC) performed in a highvolume tertiary cancer center. METHODS A retrospective review of patients undergoing esophagectomy was performed and patients with c T1 N0 and c T2 N0 esophageal cancer by EUS were evaluated. Patient demographics, tumor characteristics, and treatment were reviewed. EUS staging was compared to surgical pathology to determine accuracy of EUS. Descriptive statistics was used to describe the cohort. Student's t test and Fisher's exact test or χ~2 test was used to compare variables. Logistic regression analysis was used to determine if clinical variables such as tumor location and tumor histology were associated with EUS *** Between 2000 and 2015, 139 patients with clinical stage Ⅰ or Ⅱ?A esophageal cancer undergoing esophagectomy were identified. There were 25(18%) female and 114(82%) male patients. The tumor location included the middle third of the esophagus in 11(8%) and lower third and gastroesophageal junction in 128(92%) patients. Ninety-three percent of patients had adenocarcinoma. Preoperative EUS matched the final surgical pathology in 73/139 patients for a concordance rate of 53%. Twenty-nine patients(21%) were under-staged by EUS; of those, 19(14%) had unrecognized nodal disease. Positron emission tomography(PET) was used in addition to EUS for clinical staging in 62/139 patients. Occult nodal disease was only found in 4 of 62 patients(6%) in whom both EUS and PET were negative for nodal involvement. CONCLUSION EUS is less accurate in early EC and endoscopic mucosal resection might be useful in certain settings. The addition of PET to EUS improves staging accuracy.

关键词： Esophageal cancer Endoscopic ultrasound Staging Early esophageal cancer Endoscopic mucosal resection

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Sequence Types and Textual Production Teaching

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Sino-US English Teaching 2016年第10期13卷 783-791页

作者： gisele Gomes Guedes Xella Ohana da Cunha do Nascimento Rural Federal University of Rio de Janeiro Seropedica Brazil

Currently, Portuguese teaching prioritizes the study of text sequence types and genres. All the didactic material and activity in the classroom are guided by this study. Because the narrative text type is one of the first which the student has contact with, it will be the focus of this work. This article aims to present a didactic sequence along the lines of Dolz, Noverraz, and Schneuwly （2004）, using the fable and tale genres in order to contribute to the development and appropriation, by the students, of the structural and linguistic characteristics of these genres and the type that makes it up. This sequence of activities is fundamental to work with text production, as it greatly contributes to the writing teaching.

关键词： sequence types text genres writing

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Exercise Training for the Elderly:Inflammaging and the Central Role for HSP70

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Journal of Science in Sport and Exercise 2019年第2期1卷 97-115页

作者： Carlos Henrique de Lemos Muller Jorge Roberto de Matos gisele Bettú Grigolo Helena Trevisan Schroeder Josianne Rodrigues-Krause Mauricio Krause Laboratory of Inflammation Metabolism and Exercise Research(LAPIMEX)and Laboratory of Cellular PhysiologyDepartment of PhysiologyInstitute of Basic Health SciencesFederal University of Rio Grande do SulSarmento Leite Street500Lab 0290050-170 Porto-AlegreRSBrazil School of Physical Education Physiotherapy and DanceFederal University of Rio Grande do SulPorto AlegreRSBrazil

Inflammation is a common feature of aging tissues,being involved in most,if not all,age-related *** origin of a low-grade inflammation state in aging(inflammaging)is multifactorial and may involve changes in body composition,immunosenescence,autophagy,microbiota modification and loss of *** heat shock response pathway(HSR,and HSP70 expression)plays an important role as a mechanism of resolution of inflammation and proteostasis *** this review,we sought to discuss the mechanisms that may lead to inflammaging,and the importance of the HSP70 in this ***,we also discuss how physical exercise,particularly resistance training,can improve the HSR and the inflammatory balance of elderly people.

关键词： Aging Inflammation Exercise training Cytokines Heat shock response HSP70

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氯丙嗪对大肠杆菌细胞被膜蛋白的影响

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国外医药（抗生素分册） 1993年第3期14卷 216-217页

作者： amaral L 林风

1959年首次报道精神病治疗药吩噻嗪氯丙嗪(CPZ)有抗细菌活性,1961年证实了这个结果。最近研究表明,CPZ 有抗广谱细菌的活性,但对其抗菌机理尚不了解。因此,我们进行了以下研究。把大肠杆菌接种于胰蛋白酶黄豆汁中,过夜培养至 OD_(578nm)... 详细信息

1959年首次报道精神病治疗药吩噻嗪氯丙嗪(CPZ)有抗细菌活性,1961年证实了这个结果。最近研究表明,CPZ 有抗广谱细菌的活性,但对其抗菌机理尚不了解。因此,我们进行了以下研究。把大肠杆菌接种于胰蛋白酶黄豆汁中,过夜培养至 OD_(578nm)=1.500。分别从此培养液中约取100000CFU 接种于含有0～100μg/ml不同浓度 CPZ 的一系列胰蛋白酶黄豆汁试管中。经37℃培养,不同间隔时间测定光密度,同时吸取0.1ml 进行显微镜检查。培养到3h,在含有40～60μg/mlCPZ 的培养液中出现丝状形态的大肠杆菌;到5h,所有菌体都呈丝状,且长达20μm;到8h,培养液中杆状和丝状细胞并存;到24h,所有细胞均成杆状。

关键词：氯丙嗪大肠杆菌细胞被膜蛋白

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Ecophysiological acclimatization to cyclic water stress in Eucalyptus

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Journal of Forestry Research 2020年第3期31卷 797-806页

作者： Rogerio de Souza Noia Junior Genilda Canuto amaral Jose Eduardo Macedo Pezzopane Mariana Duarte Silva Fonseca Ana Paula Camara da Silva Talita Miranda Teixeira Xavier Department of Biosystems Engineering ESALQ University of Sao PauloPiracicabaSao PauloBrazil Department of Forestry Sciences NEDTEC Federal University of Espirito SantoJero nimo MonteiroEspirito SantoBrazil

Drought is considered the main environmental factor limiting productivity in eucalyptus plantations in Brazil. However, recent studies have reported that exposure to water deficit conditions enables plants to respond to subsequent stresses. Thus, this study investigates the ecophysiological acclimatization of eucalyptus clones submitted to recurrent water deficit cycles. Eucalyptus seedlings were submitted to three recurrent water deficit cycles and anatomical, morphological and physiological changes were analyzed. The results were:(1) Eucalyptus seedlings responded to water deficits by directing carbohydrates to root and stem growth;(2) Size and number of stomata were reduced;(3) Stomatal conductance decreased which allowed the plants to reduce water losses through transpiration,increasing instantaneous water use efficiency;(4) The relationship between gas exchanges and available water contents allowed the seedlings to uptake the retained soil water athigher tensions;and,(5) Physiological recovery from subsequent water deficits became faster. As a result of these changes, the eucalyptus seedlings recovered from the same degree of water stress more rapidly.

关键词： Carbon partition Drought Gas exchange Morpho-physiological changes Photosynthetic apparatus

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Topics on Solid Angles

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Journal of Applied Mathematics and Physics 2019年第10期7卷 2423-2439页

作者： L. C. M. amaral Dallas TX USA

The purpose of the present work is to derive some solutions for several solid angle cases via a fundamental formula which gives the solid angle for an isosceles triangle. From this formula the solid angle of pyramids is derived but, unlike other presentations, it is shown in a format similar to that of the well-known cone case. Besides the regular polygon cases (straight pyramids), solid angles of some other plane closed curves are calculated. The fundamental formula also leads to some interesting properties showing the not simple behavior of solid angles with the observer point on the curve itself, as it depends on how the observer arrived there. The question of the equi-Ω surfaces is also discussed and calculated in simple cases.

关键词： Solid Angles Pyramid Solid Angles Electric Field Flux Equi-Ω Surfaces

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Fatty acid content in epididymal fluid and spermatozoa during sperm maturation in dogs

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Journal of Animal Science and Biotechnology 2017年第3期8卷 574-581页

作者： Daniel S.Ramos Angrimani Marcilio Nichi Joāo Diego A.Losano Cristina F.Lucio gisele A.Lima Veiga Márcia V.M.Junqueira Franco Camila I.Vannucchi Department of Animal Reproduction School of Veterinary Medicine and Animal Science University of Sāo Paulo Rua Prof. Orlando Marques de Paiva 87 Sāo Paulo 05508-270 Brazil Department of Internal Medicine Division of Nutrition University of Sāo Paulo Av. Bandeirantes 3900 - 14040-040 Ribeirāo Preto. Brazil

Background： During sperm maturation, there is a reorganization of fatty acids from plasmatic membrane of the spermatozoa, which allows higher membrane integrity and acquisition of sperm motility. However, the fatty acid profile during sperm maturation remains unclear in dogs. Thus, the aim of this study was to identify the fatty acids from the epididymal spermatozoa and plasma during the sperm maturation, and observed changes in the motility and plasmatic membrane parameters. Twenty one adult dogs were used, subsequently to bilateral orchiectomy and epididymal storage, sperm samples were collected from the different segments of the epididymis. Samples were evaluated for conventional microscopy, computer-assisted motility analysis, sperm plasma membrane permeability and the fatty acid analysis（lipids were extracted, transmethylated and analyzed by chromatography）.Results： Caput and corpus sperm showed lower values for the motility variables evaluated and plasmatic membrane integrity, indicating different levels of the fatty acids organization. Saturated, monounsaturated and polyunsaturated fatty acids were in higher concentrations in the spermatozoa from epididymis cauda. Highlighting the presence of caprylic, stearic and docosahexaenoic ***： These findings demonstrate the influence of the fatty acid profile during sperm maturation, assigning physical and chemical changes in sperm cells, essential for fertilization.

关键词： Dogs Epididymis Fatty acids Sperm maturation

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Evaluation of Biodegradation in Aqueous Medium of Poly(Hydroxybutyrate-Co-Hydroxyvalerate)/Carbon Nanotubes Films in Respirometric System

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Journal of Renewable Materials 2019年第2期7卷 117-128页

作者： Larissa Stieven Montagna Isabela CÉSAR Oyama Rita de CÁSSIA Barbosa Camargo Lamparelli Ana Paula Silva THAIS Larissa do amaral Montanheiro Ana Paula Lemes Federal University of São Paulo(UNIFESP) Institute of Science and TechnologyTechnology Laboratory of Polymers and Biopolymers(TecPBio)Talim330São Josédos CamposSP12231-280BrazilSão Josédos CamposSPBrazil

Biodegradable polymers have been increasingly used for scientific and commercial applications because they are similar to some conventional thermoplastics and exhibit the ability of ***(3-hydroxybutyrate-co-3-hydroxyvalerate)(PHBV)nanocomposites films with 1 and 2 wt% of carbon nanotubes(CNT)were prepared by solution mixing,followed by solvent *** this work,PHBV/CNT nanocomposites were submitted to biodegradation in an aqueous medium for 34 days through a respirometric ***,the PHBV films were analyzed by the CO2 production and mineralization as a response of a microbial attack,which was monitored by back titration during all the *** films were also characterized by measuring the weight loss;crystallinity was evaluated by differential scanning calorimetry(DSC)and the surface morphology by scanning electron microscopy(SEM).By analyzing the weight loss of the films,it was observed that adding CNT increases the resistance to biodegradation *** obtained values of CO2 production and mineralization of the samples,as well as the values of weight loss,showed that the biodegradation of PHBV/CNT nanocomposites was minor in comparison to neat *** addition of CNT in PHBV matrix influences the surface morphology,causing the presence of cavities and an increase of roughness.

关键词： Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) carbon nanotube nanocomposites biodegradation in aqueous medium

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