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Antegrade scrotal sclerotherapy of internal spermatic veins for varicocele treatment： technique, complications, and results

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Asian Journal of Andrology 2016年第2期18卷 292-295,J0007,J0008页

作者： Alessandro Crestani Gianluca Giannarini Mattia Calandriello Marta Rossanese Mariangela Mancini giacomo Novara Vincenzo Ficarra Academic Medical Centre Hospital Santa Maria della Misericordia Udine Italy Department of Oncologic Surgical and Gastrointestinal Sciences Urologic Unit PadovaItaly Department of Experimental and Clinic Medical Sciences Urology Unit University of Udine Udine Italy.

Varicocele repair is mainly indicated in young adult patients with clinical palpable varicocele and abnormal semen parameters. Varicocele treatment is associated with a significant improvement in sperm concentration, motility, morphology, and pregnancy rate. Antegrade scrotal sclerotherapy （ASS） represented one of the main alternatives to the traditional inguinal or suprainguinal surgical ligation. This article reviews the use of ASS for varicocele treatment. We provide a brief overview of the history of the procedure and present our methods used in ASS. In addition, we review complication and success of ASS, including our own retrospective data of treating 674 patients over the last 17 years. Herein, we analyzed step by step the ASS technique and described our results with an original modified technique with a long follow-up. Between December 1997 and December 2014, we performed 674 ASS. Mean operative time was 14 min （range 9 to 50 min）. No significant intraoperative complications were reported. Within 90 days from the procedure, postoperative complications were recorded in overall 49 （7.2%） patients. No major complications were recorded. A persistent/recurrent varicocele was detected in 40 （5.9%） cases. In 32/40 （80%） cases, patients showed preoperative grade III varicoceles. In patients with a low sperm number before surgery, sperm count improved from 13 x 106 to 21 x 106 m1-1 （P〈 0.001）. The median value of the percentage of progressive motile forms at 1 h improved from 25% to 45% （P 〈 0.001）. Percentage of normal forms increased from 17% before surgery to 35% 1 year after the procedure （P 〈 0.001）. In the subgroup of the 168 infertile patients, 52 （31%） fathered offspring at a 12-month-minimum follow-up. Therefore, ASS is an effective minimal invasive treatment for varicocele with low recurrence/persistence rate.

关键词： antegrade scrotal sclerotherapy male infertility outcomes varicocele

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Lattice Boltzmann Models with Mid-Range Interactions

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Communications in Computational Physics 2007年第6期2卷 1071-1084页

作者： giacomo Falcucci Gino Bella Giancarlo Chiatti Sergio Chibbaro Mauro Sbragaglia Sauro Succi Department of Mechanical and Industrial Engineering University of“Roma Tre”Via della Vasca Navale 7900146 RomeItaly Department of Mechanical Engineering University of“Tor Vergata”Viale PolitecnicoRomeItaly Istituto Applicazioni Calcolo CNRV.le del Policlinico 13700161RomeItaly Faculty of Applied Sciences Impact and BurgerscentrumUniversity of TwenteP.O.Box 217AE 7500 EnschedeThe Netherlands.

An extension of the standard Shan-Chen model for non ideal-fluids,catering for mid-range,soft-core and hard-core repulsion,is *** is shown that the inclusion of such mid-range interactions does not yield any visible enhancement of the density jump across the dense and light *** an enhancement can however be obtained by tuning the exponents of the effective *** results also indicate that the inclusion of soft-core repulsion can prevent the coalescence of neighborhood bubbles,thereby opening the possibility of tailoring the size of multi-droplet configurations,such as sprays and related phase-separating fluids.

关键词： Lattice-Boltzmann pseudopotential phase-separation.

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Is bigger better？ Male body size affects wing-borne courtship signals and mating success in the olive fruit fly, Bactrocera oleae （Diptera： Tephritidae）

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Insect Science 2016年第6期23卷 869-880页

作者： Giovanni Benelli Elisa Donati Donato Romano giacomo Ragni Gabriella Bonsignori Cesare Stefanini Angelo Canale Insect Behaviour Group Department of Agriculture Food and Environment University of Pisa Pisa The BioRobotics Institute Sant'Anna School of Advanced Studies Pontedera Italy

Variations in male body size are known to affect inter- and intrasexual selection outcomes in a wide range of animals. In mating systems involving sexual signaling before mating, body size often acts as a key factor affecting signal strength and mate choice. We evaluated the effect of male size on courtship displays and mating success of the olive fruit fly, Bactrocera oleae （Diptera： Tephritidae）. Wing vibrations performed during successful and unsuccessful courtships by large and small males were recorded by high-speed videos and analyzed through frame-by-frame analysis. Mating success of large and small males was investigated. The effect of male-male competition on mating success was evaluated. Male body size affected both male courtship signals and mating outcomes. Successful males showed wing-borne signals with high frequencies and short interpulse intervals. Wing vibrations displayed by successful large males during copulation attempt had higher frequencies over smaller males and unsuccessful large males. In no-competition conditions, large males achieved higher mating success with respect to smaller ones. Allowing large and small males to compete for a female, large males achieve more mating success over smaller ones. Mate choice by females may be based on selection of the larger males, able to produce high-frequency wing vibrations. Such traits may be indicative of ＂good genes,＂ which under sexual selection could means good social-interaction genes, or a good competitive manipulator of conspecifics.

关键词： communication channels courtship call male-male competition mate choice sexual selection social resources

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Applications of endoscopic ultrasound elastography in pancreatic diseases: From literature to real life

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World Journal of Gastroenterology 2022年第9期28卷 909-917页

作者： Clara Benedetta Conti giacomo Mulinacci Raffaele Salerno Marco Emilio Dinelli Roberto Grassia Interventional Endoscopy Unit ASST MonzaOspedale San GerardoMonza 20900Italy Gastroenterology and Endoscopy Unit ASST Fatebenefratelli-SaccoMilan 20121Italy Gastroenterology and Endoscopy Unit ASST CremonaCremona 26100Italy

Elastography is a non-invasive method widely used to measure the stiffness of the tissues,and it is available in most endoscopic ultrasound machines,using either qualitative or quantitative *** ultrasound elastography is a tool that should be applied to obtain a complementary evaluation of pancreatic diseases,together with other imaging tests and clinical *** can be informative,especially when studying pancreatic masses and help the clinician in the differential diagnosis between benign or malignant ***,further studies are necessary to standardize the method,increase the reproducibility and establish definitive cut-offs to distinguish between benign and malignant pancreatic ***,even if promising,elastography still provides little information in the evaluation of benign conditions.

关键词： Elastography Pancreas Pancreatic stiffness Pancreatitis Pancreatic cancer Endosonography Endoscopic ultrasound Quantitative elastography Strain elastography Pancreatic diseases

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Role of Long Non-coding RNAs in Reprogramming to Induced Pluripotency

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Genomics, Proteomics & Bioinformatics 2020年第1期18卷 16-25页

作者： Shahzina Kanwal Xiangpeng Guo Carl Ward giacomo Volpe Baoming Qin Miguel A.Esteban Xichen Bao Joint School of Life Sciences Guangzhou Medical University and Guangzhou Institutes of Biomedicine and HealthGuangzhou 511436China Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine Guangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou 510530China Laboratory of RNA Chromatinand Human DiseaseGuangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou 510530China Guangzhou Regenerative Medicine and Health Guangdong Laboratory(GRMH-GDL) Guangzhou 510005China Laboratory of Metabolism and Cell Fate Guangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou 510530China Institute for Stem Cells and Regeneration Chinese Academy of SciencesBeijing 100101China Laboratory of RNA Molecular Biology Guangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou 510530China

The generation of induced pluripotent stem cells through somatic cell reprogramming requires a global reorganization of cellular *** reorganization occurs in a multi-phased manner and involves a gradual revision of both the epigenome and *** studies have shown that the large-scale transcriptional changes observed during reprogramming also apply to long noncoding RNAs(lncR NAs),a type of traditionally neglected RNA species that are increasingly viewed as critical regulators of cellular *** understanding of lncR NAs in reprogramming may not only help to improve this process but also have implications for studying cell plasticity in other contexts,such as development,aging,and *** this review,we summarize the current progress made in profiling and analyzing the role of lncR NAs in various phases of somatic cell reprogramming,with emphasis on the re-establishment of the pluripotency gene network and X chromosome reactivation.

关键词： Mesenchymal-to-epithelial transition X chromosome reactivation Apoptosis Proliferation

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Perioperative thrombotic complications in liver transplantation

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World Journal of Gastroenterology 2015年第26期21卷 8004-8013页

作者： Paolo Feltracco Stefania Barbieri Umberto Cillo giacomo Zanus Marco Senzolo Carlo Ori Department of Medicine UO Anaesthesia and Intensive CarePadua University Hospital Hepatobiliary Surgery and Liver Transplant Unit Department of SurgeryOncology and GastroenterologyUniversity Hospital of Padua Multivisceral Transplant Unit Department of SurgeryOncology and GastroenterologyUniversity Hospital of Padua

Although the perioperative bleeding complications and the major side effects of blood transfusion have always been the primary concern in liver transplantation(OLT),the possible cohesion of an underestimated intrinsic hypercoagulative state during and after the transplant procedure may pose a major threat to both patient and graft *** during OLT is characterized not only by a complex aetiology,but also by unpredictable onset and evolution of the *** initiation of a procoagulant process may be triggered by various factors,such as inflammation,venous stasis,ischemia-reperfusion injury,vascular clamping,anatomical and technical abnormalities,genetic factors,deficiency of profibrinolytic activity,and platelet *** involvement of the arterial system,intracardiac thrombosis,pulmonary emboli,portal vein thrombosis,and deep vein thrombosis,are among the most serious thrombotic events in the perioperative *** rapid detection of occlusive vascular events is of paramount importance as it heavily influences the prognosis,particularly when these events occur intraoperatively or early after *** of the lack of studies and guidelines on anticoagulant prophylaxis in this setting,many institutions recommend such an approach especially in the subset of patients at high ***,the decision of when,how and in what doses to use the various chemical anticoagulants is still a difficult task,since there is no common consensus,even for highrisk *** risk of postoperative thromboembolism causing severe hemodynamic events,or even loss of graft function,must be weighed and compared with the risk of an important *** this article we briefly review the risk factors and the possible predictors of major thrombotic complications occurringin the perioperative period,as well as their incidence and clinical ***,the indications to pharmacological prophylaxis and the current treatment strategies are also summarized.

关键词： Vascular complications Thromboembolicphenomena Liver transplantation Hepatic arteryocclusion Postoperative complications Pulmonaryemboli

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Laparoscopic hysterectomy as optimal approach for 5400 grams uterus with associated polycythemia: A case report

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World Journal of Clinical Cases 2019年第19期7卷 3027-3032页

作者： Antonio Macciò giacomo Chiappe Fabrizio Lavra Elisabetta Sanna Romualdo Nieddu Clelia Madeddu Department of Gynecologic Oncology Azienda Ospedaliera BrotzuCagliari 09100Italy Department of Medical Sciences and Public Health Medical Oncology UnitUniversity of CagliariCagliari 09042Italy

BACKGROUND Uterine myomas are the most common benign gynecological soft tissue tumors in women but polycythemia associated with these tumors is *** is significantly associated with risk of venous thromboembolism(VTE),which is further increased in case of a large pelvic mass and *** we report the surgical challenges in the case of an obese patient with a giant fibromatous uterus and associated *** SUMMARY A 42-year-old obese woman was referred to our department for treatment for a huge fibromatous *** suffered of pelvic pressure and abdominal distension and reported severe dyspnea from several *** analyses including hemoglobin(Hb)19.2 g/dL and hematocrit(Hct)59.7%were indicative of *** blood gas analysis showed arterial oxygen pressure(pO2)of 81.5 *** computed tomography confirmed a giant fibromatous uterus measuring 28.2 cm×17 cm×25 *** reduce the thromboembolic risk,the patient underwent low molecular weight heparin,phlebotomy twice before surgery,and we opted for a laparoscopic *** uterus weighed 5400 g and the histology confirmed a diagnosis of *** did not find increased erythropoietin levels in the supernatant of the myomatous uterine *** were no *** postoperative day 1,the Hb and the Hct levels normalized to 13.3 g/dL and 41.7%,*** blood gas analysis after surgery showed a pO2 of 144.7 *** results suggested the diagnosis of myomatous erythrocytosis *** patient was discharged on the second postoperative day in very good condition with no *** We believe this is the first reported laparoscopic hysterectomy in a patient with a giant uterus,polycythemia and *** VTE risk factors warranted a laparoscopic approach.

关键词： Hysterectomy Laparoscopy Myoma Myomatous erythrocytosis syndrome Polycythemia,huge uterus Total laparoscopic hysterectomy Case report

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Surgery in biliary lithiasis: from the traditional “open” approach to laparoscopy and the“rendezvous” technique

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Hepatobiliary & Pancreatic Diseases International 2017年第6期16卷 595-601页

作者： Giuseppe Tarantino Paolo Magistri Roberto Ballarin giacomo Assirati Antonio Di Cataldo Fabrizio Di Benedetto Hepato-Pancreato-Biliary Surgery and Liver Transplantation Unit University of Modena and Reggio Emilia 41124 ModenaItaly Department of General Surgery Sapienza-University of Rome 00189 Rome Italy Department of Surgical Sciences Organ Transplantation and Advanced Technologies University of Catania95124 Catania Italy

BACKGROUND:According to the current literature, biliary lithiasis is a worldwide-diffused condition that affects almost 20% of the general population. The rate of common bile duct stones(CBDS) in patients with symptomatic cholelithiasis is estimated to be 10% to 33%, depending on patient’s *** to stones in the gallbladder, the natural history of secondary CBDS is still not completely understood. It is not clear whether an asymptomatic choledocholithiasis requires treatment or not. For many years, open cholecystectomy with choledochotomy and/or surgical sphincterotomy and cleaning of the bile duct were the gold standard to treat both pathologies. Development of both endoscopic retrograde cholangiopancreatography(ERCP) and laparoscopic surgery,together with improvements in diagnostic procedures, influenced new approaches to the management of CBDS in association with *** SOURCES:We decided to systematically review the literature in order to identify all the current therapeutic options for CBDS. A systematic literature search was performed independently by two authors using Pub Med, EMBASE, Scopus and the Cochrane Library ***:The therapeutic approach nowadays varies greatly according to the availability of experience and expertise in each center, and includes open or laparoscopic common bile duct exploration, various combinations of laparoscopic cholecystectomy and ERCP and combined laparoendoscopic ***:Although ERCP followed by laparoscopic cholecystectomy is currently preferred in the majority of hospitals worldwide, the optimal treatment for concomitant gallstones and CBDS is still under debate, and greatly varies among different centers.

关键词： biliary obstruction bile duct stones gallstones endoscopic retrograde cholangiopancreatography laparoscopic cholecystectomy

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Current perspectives on active surveillance for patients with small renal masses

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Asian Journal of Urology 2022年第3期9卷 204-205页

作者： giacomo Rebez M.Carmen Mir Department of Urology University of TriesteTriesteItaly Department of Urology Fundacion Instituto Valenciano OncologiaValenciaSpain

Small renal mass(SRM)incidence has been increasing over last decades due to the widespread access to diagnostic tools and is now accounting for 48%e66%of all renal cell carcinoma(RCC)diagnoses[1].The optimal management of SRM is still debated since approximately 25%of T1a is benign *** role of active surveillance(AS)and the problem of SRM overtreatment are widely described in the current ***,RCC mortality has remained stable over the last 2 *** major strength of AS is the“tetrafecta”concept as it brings renal function preservation,safe oncological outcomes,and no surgical complications,and it maintains quality of *** is nowadays considered as a safe strategy for many SRMs.

关键词： renal mortality patients

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Red wine and green tea reduce H pylori- or VacA-induced gastritis in a mouse model

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World Journal of Gastroenterology 2007年第3期13卷 349-354页

作者： Paolo Ruggiero giacomo Rossi Francesco Tombola Laura Pancotto Laura Lauretti Giuseppe Del Giudice Mario Zoratti Novartis Vaccines & Diagnostics s.r.l. Research Center SienaItaly Department of Veterinary Science University of Camerino Matelica Italy Department of Biomedical Sciences University of PadovaItaly CNR Institute of Neuroscience Section for Biomembranes Padova Italy CNR Institute of Neuroscience Section for Biomembranes Padova Italy

AIM： To investigate whether red wine and green tea could exert anti-H pylori or anti-VacA activity in vivo in a mouse model of experimental infection. METHODS： Ethanol-free red wine and green tea concentrates were administered orally as a mixture of the two beverages to H pylori infected mice, or separately to VacA-treated mice. Gastric colonization and gastric inflammation were quantified by microbiological, histopathological, and immunohistochemical analyses.RESULTS： In H pylori-infected mice, the red wine and green tea mixture significantly prevented gastritis and limited the localization of bacteria and VacA to the surface of the gastric epithelium. Similarly, both beverages significantly prevented gastric epithelium damage in VacA-treated mice; green tea, but not red wine, also altered the VacA localization in the gastric epithelium. CONCLUSION： Red wine and green tea are able to prevent H pylori-induced gastric epithelium damage, possibly involving VacA inhibition. This observation supports the possible relevance of diet on the pathological outcome of Hpylori infection.

关键词： VacA H pylori Gastritis Wine Tea Polyphenols

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