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作者： ghulam mustafa wassan 华中农业大学

学位级别：硕士

Verticillium wilt of cotton （Gossypium hirsutum） is a widespread and destructive disease that is caused by the soil-borne fungus pathogen Verticillium dahliae. Jasmonic acid （JA） and Salicylic acid （SA） are essential signaling molecules that coordinate the plant response to biotic and abiotic challenges, in addition to several developmental processes. The basic-helix-loop-helix （bHLH） transcription factor （TF） MYC2 has recently emerged as a master regulator of most aspects of the jasmonate （JA） signaling pathway in Arabidopsis. MYC2 coordinates JA-mediated defense responses by antagonistically regulating two different branches of the JA signaling pathway that determine resistance to pests and pathogens, respectively. In present study, a MYC2-like gene, GhMYC2-like, was used and transferred into G. hirsutum cv. YZ-1 （susceptible to V. dahliae）. The transcriptional level of GhMYC2-like was up regulated in transgenic cotton plants as compared to wild types upon JA treatments. It was also observe that GhMYC2-like up regulates the expression of those genes were involved JA-mediated response defense to pathogens. While GhMYC2-like expression was suppressed after SA treatment GhMYC2-like induced transgenic lines were subjected to insect feeding stress. Results revealed that the effects of insect feeding were very less on leaves of transgenic lines that probably due to the GhMYC2-like induced JA defense system against insects. Constitutively overexpressing of GhMYC2-like transgenic plants displayed a noteworthy tolerance against herbivores, which supports our hypothesis. Overall, these results are consistent with the observation that GhMYC2-like is a positive regulator of a subset of JA-responsive genes involved in pathogen and insects stress. Virus induced gene silencing （VIGS） is post-transcriptional gene silence technique, which offers an alternative and complimentary approach for large-scale functional analysis of individual genes by knocking down the expression of endo

关键词：黄萎病基因沉默棉花

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Rare earth based MgPm_(2)X_(4)(X=S,Se) spinel chalcogenides for spintronic and thermoelectric applications

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Journal of Rare Earths 2024年第8期42卷 1577-1585,I0006页

作者： Tariq M.Al-Daraghmeh Omar Zayed ghulam M.mustafa Taharh Zelai Bisma Younas Hind Albalawi S.Bouzgarrou Othman Hakami Q.Mahmood Khaild I.Hussein Basic Sciences Department King Abdullah Ⅱ School of EngineeringPrincess Sumaya University For TechnologyP.O.Box 1438Al-jubaiha11941AmmanJordan Department of Physics Division of Science&TechnologyUniversity of EducationLahorePakistan Department of Physics Faculty of ScienceJazan University JazanSaudi Arabia Department of Physics The University of LahoreLahorePakistan Department of Physics College of SciencesPrincess Nourah bint Abdulrahman University(PNU)P.O.Box 84428Riyadh11671Saudi Arabia Department of Physics College of ScienceQassim UniversityPo.64BuraidahSaudi Arabia Laboratoire de Microelectronique et Instrumentation(UR 03/13-04) Faculte des Sciences de MonastirAvenue de I'Environnement 5000MonastirTunisia Department of Chemistry Faculty of ScienceJazan University JazanSaudi Arabia Basic&Applied Scientific Research Center Department of PhysicsCollege of ScienceImam Abdulrahman Bin Faisal UniversityP.O.Box 1982Dammam 31441Saudi Arabia Department of Radiological Sciences College of Applied Medical SciencesKing Khalid UniversityAbha61421Saudi Arabia

In current report,the structural,magnetic,and thermoelectric properties of RE doped MgPm_(2)X_(4)(X=S,Se) spinels were *** energy difference in ferromagnetic and antiferromagnetic states reveals the stability of MgPm_(2)(S/Se)_(4) in the ferromagnetic *** co mputation of enthalpy of formation also ascertains thermodynamic stability of crystal ***-dependent band structure and density of states analysis reveal ferromagnetic semiconducting character showing different electronic behavior in both spin *** room temperature ferromagnetism,spin polarization and Curie temperature are estimated from exchange energies *** addition,exchange constants(N_(0)α and N_(0)β),exchange energy Δ_(x)(pd),crystal ifeld energy,and double exchange mechanism were studied to explore the magnetic ***,the electrical conductivity,thermal conductivity,Seebeck co-efficient,and power factor show effect on electrons spin and their potential for thermoelectric devices.

关键词： Rare earth elements Spintronic applications Thermoelectric effects Exchange energies

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GbSOBIR1通过磷酸化bHLH171介导棉花对黄萎病的抗性

GbSOBIR1通过磷酸化bHLH171介导棉花对黄萎病的抗性

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中国农学会棉花分会2018年年会

作者：周易孙龙清 ghulam mustafa wassan 何昕朱龙付张献龙作物遗传改良国家重点实验室/华中农业大学

SOBIR1在植物基础免疫中具有重要作用,并介导了番茄Ve1对1号大丽轮枝菌生理小种的抗性。但其在棉花抗黄萎病中的作用尚不明确。本研究发现1个编码类受体蛋白激酶的基因的表达在ssn组成型免疫激活棉花突变体中与野生型相比具有明显差异... 详细信息

SOBIR1在植物基础免疫中具有重要作用,并介导了番茄Ve1对1号大丽轮枝菌生理小种的抗性。但其在棉花抗黄萎病中的作用尚不明确。本研究发现1个编码类受体蛋白激酶的基因的表达在ssn组成型免疫激活棉花突变体中与野生型相比具有明显差异。克隆了该基因的全长序列,发现其编码的蛋白质多肽与拟南芥SOBIR1具有60%的同源性,并将该基因命名为GbSOBIR1。GbSOBIR1包含1个信号肽区域,5个亮氨酸富集重复(Leucine-rich repeat,LRR)区域,1个跨膜区域和1个膜内激酶区域。表达分析显示GbSOBIR1在棉花的各个组织中均有表达,并且受到黄萎病菌侵染的诱导上调表达。利用病毒介导的基因沉默(Virus induced gene silencing,VIGS)抑制GbSOBIR1表达后,棉花对大丽轮枝菌株"V991"的抗性减弱,并且抗病相关激素合成基因ICS1、LOX2和AOS以及病程相关蛋白PR1、PR5的表达量均有所下降。同时,在拟南芥中异源超量表达GbSOBIR1后,转基因植株对黄萎病的抗性得到了增强。这表明GbSOBIR1参与了植物抗黄萎病过程,并对抗病性具有正向调节功能。通过GbSOBIR1胞内蛋白激酶结构域进行酵母双杂筛选到多个互作蛋白,其中包括1个basic helix-loop-helix(bHLH)类的转录因子GbbHLH171。通过构建干涉和超量表达GbbHLH1 71的转基因株系,证明其对于棉花抗病反应具有重要作用。利用Co-IP等试验证明GbSOBIR1可以在体外和体内条件下磷酸化GbbHLH171,磷酸化后的GbbHLH171对其下游抗病相关基因的表达具有明显增强的转录活性。通过质谱分析和点突变分析进一步明确了GbSOBIR1对GbbHLH171的磷酸化位点位于Ser-413,基于这个位点的磷酸化影响了GbbHLH171的转录活性。

关键词：棉花黄萎病类受体蛋白激酶 bHLH转录因子信号转导

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GhJAZ2通过抑制bHLH171转录活性降低棉花抗病性

GhJAZ2通过抑制bHLH171转录活性降低棉花抗病性

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中国农学会棉花分会2018年年会

作者：何昕朱龙付 ghulam mustafa wassan 王钰静苗玉焕张献龙作物遗传改良国家重点实验室/华中农业大学

植物进化出多重防御机制来使自己更好地适应复杂多变的环境。茉莉酸(Jasmonic acid,JA)是植物抵御病原侵染的重要防御激素。由生物和非生物胁迫信号引起的JA积累,通过降解具有j asmon-zim结构域的负调控蛋白JAZs从而激活了信号传导的转... 详细信息

植物进化出多重防御机制来使自己更好地适应复杂多变的环境。茉莉酸(Jasmonic acid,JA)是植物抵御病原侵染的重要防御激素。由生物和非生物胁迫信号引起的JA积累,通过降解具有j asmon-zim结构域的负调控蛋白JAZs从而激活了信号传导的转录因子(如MYC2),并激活下游一系列防御反应相关基因的表达。研究发现1个编码具有JAZ结构域多肽的基因GhJAZ2在棉花JA处理和接种大丽轮枝菌时的表达均明显增强。在棉花中过量表达GhJAZ2会削弱其对JA的敏感度,降低JA响应相关基因如GhPDF1.2和GhVSP的表达水平,同时使棉花对大丽轮枝菌和食草性昆虫更敏感。酵母双杂交和双分子荧光互补(Bimolecular fluorescence complementation,BiFC)分析表明,GhJAZ2可以与更多抗病反应相关蛋白互作,如病程相关蛋白GhPR10、类过敏源蛋白GhD2、NBS-LRR类蛋白质GhR1,以及具有螺旋-环-螺旋的转录因子bHLH171。与MYC2不同的是,在棉花中过量表达bHLH171激活了JA合成和信号通路,并提高了植物对大丽轮枝菌的抗性。分子和遗传证据表明,GhJAZ2可以与bHLH171互作并抑制其转录活性,从而抑制了由JA介导的防御反应。这表明JA信号路径在棉花抗黄萎病中具有重要作用,同时棉花JA信号路径与拟南芥JA调控存在差异。

关键词：棉花黄萎病茉莉酸信号转导 bHLH转录因子

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Statistical and Geometrical Way of Model Selection for a Family of Subdivision Schemes

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Chinese Annals of Mathematics,Series B 2017年第5期38卷 1077-1092页

作者： ghulam mustafa Department of Mathematics The Islamia University of BahawalpurBahawalpur 63100Pakistan

The objective of this article is to introduce a generalized algorithm to produce the m-point n-ary approximating subdivision schemes(for any integer m, n ≥ 2). The proposed algorithm has been derived from uniform B-spline blending functions. In particular, we study statistical and geometrical/traditional methods for the model selection and assessment for selecting a subdivision curve from the proposed family of schemes to model noisy and noisy free data. Moreover, we also discuss the deviation of subdivision curves generated by proposed family of schemes from convex polygonal curve. Furthermore, visual performances of the schemes have been presented to compare numerically the Gibbs oscillations with the existing family of schemes.

关键词： Approximating subdivision schemes B-spline blending function Convex polygon Statistical and geometrical methods Model selection andassessment

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ESTIMATING ERROR BOUNDS FOR TERNARY SUBDIVISION CURVES/SURFACES

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Journal of Computational Mathematics 2007年第4期25卷 473-484页

作者： ghulam mustafa Jiansong Deng Department of Mathematics Islamia University Bahawalpur Pakistan Department of Mathematics University of Science and Technology of China Hefei 230026 China Department of Mathematics University of Science and Technology of China Hefei 230026 China

We estimate error bounds between ternary subdivision curves/surfaces and their control polygons after k-fold subdivision in terms of the maximal differences of the initial control point sequences and constants that depend on the subdivision mask. The bound is independent of the process of subdivision and can be evaluated without recursive subdivision. Our technique is independent of parametrization therefore it can be easily and efficiently implemented. This is useful and important for pre-computing the error bounds of subdivision curves/surfaces in advance in many engineering applications such as surface/surface intersection, mesh generation, NC machining, surface rendering and so on.

关键词： Subdivision curve Subdivision surface Subdivision depth Error bound.

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Optimization of Neutrino Oscillation Parameters Using Differential Evolution

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Communications in Theoretical Physics 2013年第3期59卷 324-330页

作者： ghulam mustafa Faisal Akram Bilal Masud Centre for High Energy Physics University of the PunjabLahore(54590)Pakistan

We show how the traditional grid based method for finding neutrino oscillation parameters △m2 and tan2θ can be combined with an optimization technique, Differential Evolution （DE）, to get a significant decrease in computer processing time required to obtain minimal chi-square （χ2） in four different regions of the parameter space. We demonstrate efficiency for the two-neutrinos case. For this, the χ2 function for neutrino oscillations is evaluated for grids with different density of points in standard allowed regions of the parameter space of △m2 and tan2θ using experimental and theoretical total event rates of ehlorine （Homestake）, Gallex＋GNO, SAGE, Superkamiokande, and SNO detectors. We find that using DE in combination with the grid based method with smail density of points can produce the results comparable with the one obtained using high density grid, in much lesser computation time.

关键词： solar neutrinos neutrino masses and mixing numerical optimization

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Hybridization of CNN with LBP for Classification of Melanoma Images

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Computers, Materials & Continua 2022年第6期71卷 4915-4939页

作者： Saeed Iqbal Adnan N.Qureshi ghulam mustafa Faculty of Information Technology University of Central PunjabLahorePakistan Department of Computer Sciences Bahria University Lahore CampusPakistan

Skin cancer(melanoma)is one of the most aggressive of the cancers and the prevalence has significantly increased due to increased exposure to ultraviolet ***,timely detection and management of the lesion is a critical consideration in order to improve lifestyle and reduce *** this end,we have designed,implemented and analyzed a hybrid approach entailing convolutional neural networks(CNN)and local binary patterns(LBP).The experiments have been performed on publicly accessible datasets ISIC 2017,2018 and 2019(HAM10000)with data augmentation for in-distribution *** a novel contribution,the CNN architecture is enhanced with an intelligible layer,LBP,that extracts the pertinent visual *** of Basal Cell Carcinoma,Actinic Keratosis,Melanoma and Squamous Cell Carcinoma has been evaluated on 8035 and 3494 cases for training and testing,*** outcomes with cross-validation depict a plausible performance with an average accuracy of 97.29%,sensitivity of 95.63%and specificity of 97.90%.Hence,the proposed approach can be used in research and clinical settings to provide second opinions,closely approximating experts’intuition.

关键词： Skin cancer convolutional neural network feature extraction local binary pattern classification

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Generalized and Unified Families of Interpolating Subdivision Schemes

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Numerical Mathematics(Theory,Methods and Applications) 2014年第2期7卷 193-213页

作者： ghulam mustafa Pakeeza Ashraf Jiansong Deng Department of Mathematics The Islamia University of BahawalpurPakistan University of Science and Technology of China P.R.China

We present generalized and unified families of (2n)-point and (2n − 1)-point p-ary interpolating subdivision schemes originated from Lagrange polynomialfor any integers n ≥ 2 and p ≥ 3. Almost all existing even-point and odd-pointinterpolating schemes of lower and higher arity belong to this family of schemes. Wealso present tensor product version of generalized and unified families of *** error bounds between limit curves and control polygons of schemes arealso calculated. It has been observed that error bounds decrease when complexityof the scheme decrease and vice versa. Furthermore, error bounds decrease withthe increase of arity of the schemes. We also observe that in general the continuityof interpolating scheme do not increase by increasing complexity and arity of thescheme.

关键词： Interpolating subdivision scheme even-ary schemes odd-ary schemes Lagrange polynomial parameters error bounds tensor product

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The Values and Significance of the Colonial Steel Railway Bridges of Pakistan

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Built Heritage 2017年第4期1卷 11-21页

作者： Ayesha Pamela Rogers ghulam mustafa Baloch Cultural Studies Department National College of ArtsLahorePakistan

This paper proposes that Civil Engineering Heritage is a distinct form of heritage,related to but differing from Industrial Heritage and a separate field that should be treated *** critical element that signifies engineering heritage is ingenium or innovation in development of complex technological responses to physical *** heritage requires specific approaches to its identification,documentation,conservation and *** important first step of this process is a clear understanding of the values and attributes that contribute to the significance of engineering heritage and must be *** example of engineering heritage examined here is the colonial steel railway bridges of Pakistan;three case study bridges are presented and the values and attributes of each are *** paper then identifies a set of shared essential values of this type of engineering heritage that can serve as a basis for value-based conservation and management.

关键词： civil engineering heritage bridges colonial Pakistan values innovation

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