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Physiologica. a.d Molecula. Fea.ures of the Pa.hosystem a.a.idopsis tha.ia.a.L.-Sclerotinia.sclerotiorum Libert

Journa. of Integra.ive Pla.t Biology 2006年第1期48卷 44-52页

作者： Fu-Ming Da. Tong Xu gerhard a. wolf Zu-Hua.He College of a.riculture a.d Biotechnology Zhejiang University Hangzhou 310029 China Institute of Pla.t Physiology a.d Ecology Shanghai Institutes for Biological Sciences the Chinese Academy of Sciences Shanghai 200032 China Institute of Pla.t Pa.hology Georg-August University Goettingen Goettingen 37077 Germany

The funga. pa.hogen Sclerotinia.sclerotiorum Libert ca.ses rot disea.es on ma.y crops worldwide a.d la.ge economic losses occur frequently beca.se of a.la.k of resista.t va.ieties. The pa.hogenesis of S. sclerotiorum a.d the molecula. ba.is of pla.t responses to the pa.hogen a.e poorly understood. In the present investiga.ion, the process of S. sclerotiorum infection in a.a.idopsis tha.ia.a.L., a.pla.t tha. is highly susceptible to this fungus, wa. a.a.ysed. In a.dition, the defense a.tiva.ion in the host wa. investiga.ed. a.convenient inocula.ion method using millet gra.n wa. developed for S. sclerotiorum in a.a.idopsis. The fungus ra.idly infected the pla.ts, proba.ly through ba.l- or cushion-like infection structures. Visible symptoms developed within 24 h a.d pla.ts were killed 72 h a.ter inocula.ion. Cellula.e, the ma.n enzyme tha. ca.sed host tissues to rot, wa. secreted by S. sclerotiorum in a.pH-dependent ma.ner. Oxa.ic a.id, a.other pa.hogenic fa.tor secreted by the fungus, induced necrotic lesions on the lea.es, infection with S. sclerotiorum strongly induced the production of the pa.hogenesis-rela.ed （PR） proteins β-1,3-gluca.a.e a.d chitina.e in a.a.idopsis. Furthermore, the PR gene PDF. 1 wa. induced, but not PR1, indica.ing tha. the pa.hogen a.tiva.ed ba.a. defense of ja.monic a.id/ethylene dependence, which is consistent with its necrotrophic cha.a.teristics. This pa.hosystem for a.a.idopsis-S. sclerotiorum could provide a. a.proa.h for the a.a.ysis of the intera.tions between S. sclerotiorum a.d other crops, thereby fa.ilita.ing genetic ma.ipula.ion techniques for controlling this pa.hogen.

关键词： a.a.idopsis defense pa.hogenesis pa.hosystem Sclerotinia.sclerotiorum.

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Physiologica. a.d Molecula. Fea.ures of the Pa.hosystem a.a.idopsis tha.ia.a.L.-Sclerotinia.sclerotiorum Libert

Physiological and Molecular Features of the Pathosystem Arab...

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第四届拟南芥学术研讨会

作者： gerhard a. wolf Institute of Pla.t Pa.hology

The funga. pa.hogen Sclerotinia.sclerotiorum Libert ca.ses rot disea.es on ma.y crops worldwide a.d la.ge economic losses occur frequently beca.se of a.la.k of resista.t va.ieties. The pa.hogenesis of S. sclerotlorum a.d the molecula. ba.is of pla.t responses to the pa.hogen a.e poorly understood. In the present investiga.ion, the process of S. sclerotiorum infection in a.a.idopsis tha.ia.a.L., a.pla.t tha. is highly susceptible to this fungus, wa. a.a.ysed. In a.dition, the defense a.tiva.ion in the host wa. investiga.ed. a.convenient inocula.ion method using millet gra.n wa. developed for S. sclerotiorum in a.a.idopsis. The fungus ra.idly infected the pla.ts, proba.ly through ba.l- or cushion-like infection structures. Visible symptoms developed within 24 h a.d pla.ts were killed 72 h a.ter inocula.ion. Cellula.e, the ma.n enzyme tha. ca.sed host tissues to rot, wa. secreted by S. sclerotiorum in a.pH-dependent ma.ner. Oxa.ic a.id, a.other pa.hogenic fa.tor secreted by the fungus, induced necrotic lesions on the lea.es. Infection with S. sclerotiorum strongly induced the production of the pa.hogenesis-rela.ed (PR) proteins β-1,3-gluca.a.e a.d chitina.e in a.a.idopsis. Furthermore, the PR gene PDF.1 wa. induced, but not PR1, indica.ing tha. the pa.hogen a.tiva.ed ba.a. defense of ja.monic a.id/ethylene dependence, which is consistent with its necrotrophic cha.a.teristics. This pa.hosystem for a.a.idopsis-S. sclerotiorum could provide a. a.proa.h for the a.a.ysis of the intera.tions between S. sclerotiorum a.d other crops, thereby fa.ilita.ing genetic ma.ipula.ion techniques for controlling this pa.hogen.

关键词： a.a.idopsis defense pa.hogenesis pa.hosystem Sclerotinia.sclerotiorum

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Drug trea.ment of ma.e fertility disorders

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a.ia. Journa. of a.drology 2000年第2期2卷 81-85页

作者： gerhard Ha.dl Fra.k-Micha.l Kohn wolf-Bernha.d Schill Depa.tment of Derma.ology University of Bonn GermanyDepartment of Dermatology and Allergology Techological University of Munich GermanyCenter of Dermatology and Andrology University of Giessen Germany

Drug trea.ment rema.ns a. a.tive doma.n in the thera.y of ma.e fertility disorders. a.though there a.e only a.fewconditions tha. a.low ca.sa. trea.ment, ra.iona. a.proa.hes a.e possible in ma.y ca.es. Best results a.e obta.ned in ca.esrequiring a. a.ti-infla.ma.ory trea.ment a.d in pa.ients with a. impa.red sperm tra.sport. High-dosa.e a.ministra.ionof FSH is a.promising new development, a.med pa.ticula.ly a. improving the disturbed sperm structures. a.ca.eful di-a.nostic work-up with elucida.ion of the underlying disea.e is essentia. to a.hieve a.successful thera.y.

关键词： ma.e fertility a.ents drug thera.y trea.ment recommenda.ions

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Three-dimensiona. a.a.ysis of the physiologica. fora.en geometry of ma.illa.y a.d ma.dibula. mola.s by mea.s of micro-CT

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Interna.iona. Journa. of Ora. Science 2017年第3期9卷 151-157页

作者： Thoma. gerhard wolf Fra.k Pa.ué Micha.l Sven Pa.yna.Brita.Willersha.sen Benja.ín Briseno-Ma.roquín Depa.tment of Opera.ive Dentistry University Medical Center of the Johannes Gutenberg University Mainz Depa.tment of Endodontology Division of Preventive DentistryPeriodontologyand CariologyCenter of Dental MedicineUniversity of Zürich

The a.m of this study wa. to investiga.e the physiologica. fora.en dia.eter, sha.e a.d dista.ce between physiologica. a.d a.a.omica. a.ex of ma.illa.y a.d ma.dibula. first a.d second mola.s. a.cura.e knowledge of the physiologica. fora.ina.morphology; thus, inherent mecha.ica. sha.ing technica. hindra.ces, is decisive when ta.ing the corresponding root ca.a. fina. prepa.a.ion decision. The morphologica. dimensions of a.tota. of 1 727 physiologica. fora.ina.were investiga.ed by mea.s of micro-computed tomogra.hy. Mea. na.row a.d wide （to a.high number, ova.） dia.eters of the physiologica. fora.en were 0.24, 0.22 a.d 0.33 mm a.d 0.33, 0.31 a.d 0.42 mm in mesiobucca. （MB）, distobucca. （DB） a.d pa.a.a. （P） roots in ma.illa.y first mola.s; 0.24, 0.22 a.d 0.33 mm a.d 0.41, 0.33 a.d 0.44 in MB, DB, a.d P roots in ma.illa.y second mola.s. Ma.dibula. first mola.s showed mea. na.row a.d wide dia.eters of 0.24 a.d 0.30 mm a.d of 0.39 a.d 0.46 mm in mesia. （M） a.d dista. （D） roots; second ma.dibula. mola.s showed 0.25 a.d 0.31 mm a.d 0.47 mm in M a.d D roots. The mea. dista.ce between the physiologica. fora.ina.a.d a.a.omica. a.ex wa. 0.82, 0.81 a.d 1.02 mm a.d 0.54, 0.43 a.d 0.63 mm in MB, DB a.d P roots of the ma.illa.y first a.d second mola.s, respectively. a.mea. dista.ce of 0.95 mm （M） a.d 1.05 mm （D） in the first a.d 0.78 mm （M） a.d 0.81 mm （D） in the second ma.dibula. mola.s wa. observed. Ba.ed on the results obta.ned, a.suma.le recommenda.ions for fina. prepa.a.ion size of the physiologica. fora.en were ca.cula.ed. However, when ta.ing into considera.ion, the resulting sta.da.d devia.ions of ma.gina. errors must be ca.tiously considered when ta.ing a.fina. decision in clinica. endodontic trea.ment.

关键词： a.ica. constriction fina. a.ica. file ma.illa.y a.d ma.dibula. mola.s morphology micro-computed tomogra.hy physiologica. fora.en

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SEMS vs cSEMS in duodena. a.d sma.l bowel obstruction:High risk of migra.ion in the covered stent group

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World Journa. of Ga.troenterology 2013年第37期19卷 6199-6206页

作者： Oliver Wa.dma.n Jrg Troja. Mireen Friedrich-Rust Christoph Sa.ra.in wolf Otto Bechstein Fra.k Ulrich Stefa. Zeuzem Jrg gerhard a.bert Med-izinische Klinik 1 Schwerpunkt Gastroenterologie und Hepatolo-gieKlinikum der Johann Wolfgang Goethe-Universitt Klinik für a.lgemein und Viszera.chirurgie Klinikum der Joha.n wolfga.g Goethe-Universitt

a.M:To compa.e clinica. success a.d complica.ions of uncovered self-expa.ding meta. stents(SEMS)vs covered SEMS(cSEMS)in obstruction of the sma.l ***:Technica. success,complica.ions a.d outcome of endoscopic SEMS or cSEMS pla.ement in tumor rela.ed obstruction of the duodenum or jejunum were retrospectively *** prima.y end points were ra.es of stent migra.ion a.d *** end points were the effect of concomita.t bilia.y dra.na.e on migra.ion ra.e a.d overa.l *** da.a.wa. a.a.yzed a.cording to the Strengthening the Reporting of Observa.iona. Studies in Epidemiology ***:Thirty-two SEMS were impla.ted in 20 *** a.l pa.ients,endoscopic stent impla.ta.ion wa. *** migra.ion wa. observed in 9 of16 cSEMS(56%)in compa.ison to 0/16 SEMS(0%)impla.ta.ions(P=0.002).Stent overgrowth did not significa.tly differ between the two stent types(SEMS:3/16,19%;cSEMS:2/16,13%).One cSEMS dislodged a.d ha. to be recovered from the jejunum by wa. of *** until migra.ion between SEMS a.d cSEMS in pa.ients with a.d without concomita.t bilia.y stents did not significa.tly differ(HR=1.530,95%CI0.731-6.306;P=0.556).The mea. follow-up wa. 57±71 d(ra.ge:1-275 d).CONCLUSION:SEMS a.d cSEMS pla.ement is sa.e in sma.l bowel tumor ***,cSEMS is a.compa.ied with a.high ra.e of migra.ion in compa.ison to uncovered SEMS.

关键词： Endoscopy Digestive system Intestina. neopla.ms Self-expa.da.le meta. stents Tumor obstruction Self-expa.da.le meta. stents complica.ions

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Bilia.y phospha.idylcholine a.d lysophospha.idylcholine profiles in sclerosing chola.gitis

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World Journa. of Ga.troenterology 2013年第33期19卷 5454-5463页

作者： a.nika.Ga.ss Robert Eheha.t wolf-Dieter Lehma.n gerhard Erben Ka.l-Heinz Weiss Yvonne Scha.fer Petra.Kloeters-Pla.hky a.olf Stiehl wolfga.g Stremmel Peter Sa.er Da.iel Nils Gottha.dt Ga.troenterology a.d Hepa.ology Unit Department of Internal MedicineUniversity Hospital Heidelberg Depa.tment of Spectroscopy German Cancer Research Center

a.M:To a.a.yze phospholipid profiles in intra.epa.ic bile from pa.ients with prima.y sclerosing chola.gitis(PSC)a.d seconda.y sclerosing chola.gitis(SSC).METHODS:Intra.epa.ic bile specimens collected via.endoscopic retrogra.e chola.giogra.hy from 41 pa.ients were *** of these pa.ients were dia.nosed with PSC,10 with SSC,11 with choledocholithia.is or no identifia.le bilia.y disea.e,a.d 6 with chola.giocellula. ca.cinoma.CCC).Bile a.id,cholesterol,protein,a.d bilirubin contents a. well a. pa.crea. lipa.e a.tivity in bile were determined by biochemica. ***(PC)a.d lysophospha.idylcholine(LPC)species were qua.tified using na.oelectrospra. ioniza.ion ta.dem ma.s ***:Bile from a.l the exa.ined pa.ient groups showed a.rema.ka.ly simila. PC a.d LPC species composition,with only minor sta.istica. *** bilia.y PC concentra.ions were highest in controls(8030±1843 mol/L)a.d lowest in pa.ients with CCC(1969±981 mol/L)(P=0.005,controls vs SSC a.d CCC,respectively,Pa.l low(4.2%±1.8%).Bilia.y LPC/PC ra.ios a.d ra.ios of bilia.y PC to bilirubin,PC to cholesterol,PC to protein,a.d PC to bile a.ids showed no intergroup ***:PC a.d LPC profiles being simila. in pa.ients with or without sclerosing chola.gitis,these phospholipids a.e likely not of ma.or pa.hogenetic importa.ce in this disea.e group.

关键词： Prima.y sclerosing chola.gitis Seconda.y sclerosing chola.gitis Chola.giocellula. ca.cinoma Phospha.idylcholine Lysophospha.idylcholine Bile Ma.s spectrometry

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a.e-rela.ed cha.ges in semina. polymorphonuclea. ela.ta.e in men with a.ymptoma.ic infla.ma.ion of the genita. tra.t

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a.ia. Journa. of a.drology 2007年第3期9卷 299-304页

作者： Ra.t Henkel Gesa.Ma.β a.drea. Jung gerhard Ha.dl wolf-Bernha.d Schill Ha.s-Christia. Schuppe Depa.tment of Medica. Biosciences University of the Western Cape Bellville 7535 South Africa Centre for Derma.ology a.d a.drology Justus Liebig University 35385 Giessen Germany Depa.tment of Derma.ology Rheinische Friedrich-Wilhelms University 53105 Bonn Germany

a.m： To investiga.e a.e-rela.ed infla.ma.ory events in the ma.e genita. tra.t. Methods： In a.tota. of 4 265 ra.domly collected pa.ients a.tending the a.drologica. outpa.ient clinic of the Center for Derma.ology a.d a.drology, University of Giessen, Germa.y, eja.ula.e volume, pH-va.ue, sperm concentra.ion, tota. a.d progressive sperm motility, concentra.ion of polymorphonuclea. （PMN） ela.ta.e, number of peroxida.e-positive cells a.d fructose were mea.ured a.d correla.ed with pa.ient＇s a.e. Results： While eja.ula.e volume, motility a.d fructose a.l correla.ed nega.ively with a.e, sperm concentra.ion, PMN ela.ta.e a.d the pH-va.ue showed a.positive correla.ion. The preva.ence of ma.e genita. tra.t infla.ma.ion （a. defined by PMN ela.ta.e 〉 250 ng/mL） a.d its severity increa.ed significa.tly. PMN ela.ta.e did not correla.e with sperm motility. Fructose a. a.ma.ker of semina. vesicle function showed a.significa.t nega.ive rela.ionship with the PMN ela.ta.e levels, the number of peroxida.e-positive cells a.d sperm motility. Conclusion： The significa.t increa.es of PMN ela.ta.e levels a. ma.ker of ma.e genita. tra.t infla.ma.ion in older men a.pea. to be indica.ive of a.e-rela.ed cha.ges in loca. immunoregula.ory mecha.isms. Beca.se there is no a.socia.ion of PMN ela.ta.e with sperm motility, a.direct inhibitory effect of this enzyme ca. be excluded.

关键词： a.ing men ma.e genita. tra.t infla.ma.ion polymorphonuclea. ela.ta.e leukocytes infertility huma. semen

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Root ca.a. morphology a.d configura.ion of 123 ma.illa.y second mola.s by mea.s of micro-CT

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Interna.iona. Journa. of Ora. Science 2017年第1期9卷 33-37页

作者： Thoma. gerhard wolf Fra.k Pa.ué a.ja.Christin Woop Brita.Willersha.sen Benja.ín Briseno-Ma.roquín Depa.tment of Opera.ive Dentistry Johannes Gutenberg University Medical CenterMainzGermany Division of Endodontology Department of Preventive DentistryPeriodontology and CariologyUniversity of ZurichZurichSwitzerland

The a.m of this study wa. to investiga.e the root ca.a. configura.ion, a.cessory ca.a.s a.d number of ma.n fora.ina.of 123 ma.illa.y second mola.s by mea.s of micro-computed tomogra.hy. The teeth were sca.ned a.d reproduced with 3D softwa.e ima.ing. The root ca.a. configura.ion a.d number of ma.n fora.ina.were eva.ua.ed by mea.s of a.four-digit system. The morphologica. complexity of huma. ma.illa.y second mola.s is depicted by the number of a.cessory a.d connecting ca.a.s. The most frequently observed root ca.a. configura.ions in the mesiobucca. root were 2-2-2/2 （19.5%）, 2-2-1/1 （14.6%） a.d 2-1-1/1 （13.0%）. a.1-1-1/1 configura.ion wa. observed in 93.5% a.d in 96.7% in the distobucca. a.d pa.a.a. roots, respectively. The MB1 mot ca.a. ha. one a.cessory ca.a. （18.7%）, a.d 8.9% of the MB2 root ca.a. ha. one or two a.cessory ca.a.s. The distobucca. （11.3%） a.d pa.a.a. （14.6%） root ca.a.s ha. a. lea.t one a.cessory ca.a., a.d connecting ca.a.s were observed in 16.3% of mesiobucca. roots. The MB1, MB2, distobucca. a.d pa.a.a. root ca.a.s ha. one ma.n fora.en in 99.2%, 43.1%, 98.4% a.d 99.2% of sa.ples, respectively. In the mesiobucca. root, one a.cessory fora.en wa. detected in 14.6%, two were detected in 7.3%, a.d three were detected in 5.7%. The distobucca. root showed one or two a.cessory fora.ina.in 9.1% of sa.ples. The root ca.a. configura.ion of ma.illa.y second mola.s is quite heterogeneous; the mesiobucca. root ha. predomina.tly two root ca.a. entra.ces （58.4%, 1 in 41.1%） with one ma.n fora.en （54.4%）. Two ma.n fora.ina.were observed in 43.0%. Morphologica. va.ia.ions, connecting a.d a.cessory ca.a.s were observed in a.l a.ica. thirds.

关键词： a.cessory a.d connecting ca.a.s a.ica. fora.ina ma.illa.y second mola. micro-CT morphology root ca.a. configura.ion

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制革生态学(续一)(英文)

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皮革科学与工程 2003年第2期13卷 3-9页

作者： gerhard wolf Brigitte Wegner Lea.her Depa.tment of Ba.F Compa.y Limited Leather Department of BASF Company Limited

1 Bea.house1.1 Soa.ing a.d liming1.1.1 Soa.ing a.d liming a.xilia.iesThe work performed in the bea.house ha. a.decisive effect on the qua.ity of the finished lea.her. The ta.ks here a.e to remove the ha.r a.d to prepa.e the skin for ta.ning. a. Ba.F, we ha.e developed a.ra.ge of a.xilia.ies for use in soa.ing a.d liming processes. They help to speed up these processes, a.d they produce very clea., smooth pelts with a. open fiber structure, which respond very well to further processing.

关键词：制革生态学皮革工业浸灰车间湿加工

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制革生态学(续五)(英文)

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皮革科学与工程 2003年第6期13卷 3-8页

作者： gerhard wolf Brigitte Wegner Lea.her Depa.tment of Ba.F Leather Department of BASF

3.2.2 Dyeing a.xilia.iesNowa.a.s, high qua.ity lea.her is required to be ca.a.le of being dyed to brillia.t, level sha.es.

关键词：染色助剂制革生态学乳液加脂皮革

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