检索结果-南通市图书馆

Highly Luminescent Positively Charged Quantum Dots Interacting with Proteins and Cells

Chinese Journal of Chemistry 2022年第22期40卷 2685-2693页

作者： Haixia Wang Karin nienhaus Li Shang gerd ulrich nienhaus Institute of Applied Physics Karlsruhe Institute of Technology(KIT)76131 KarlsruheGermany Institute of Nanotechnology Karlsruhe Institute of Technology(KIT)76344 Eggenstein-LeopoldshafenGermany InstituteofBiologicaland Chemical Systems Karlsruhe Institute of Technology(KIT)76344 Eggenstein-LeopoldshafenGermany Department of Physics University of llinois at Urbana-ChampaignUrbanaIL61801USA Present address:Laboratory of Biomaterials and Translational Medicine Center for NanomedicineThe Third Affiliated HospitalSun Yat-sen UniversityGuangzhou510630China Present address:School of Materials Science and Engineering Northwestern PolytechnicalUniversityXi'an 710072China

We have studied interactions between positively charged MUTAB-stabilized quantum dots(QDs)and model proteins,serum and live cells using fluorescence correlation spectroscopy(FCS),dynamic light scattering(DLS),time-resolved photoluminescence(PL)and live-cell fluorescence *** human serum albumin(HSA)as a model protein,we measured the growth of a protein adsorption layer(“protein corona”)via time-resolved *** formation was characterized by an apparent equilibrium dissociation coefficient,KD≈10μ*** adlayer growth was surprisingly slow(timescale ca.30 min),in stark contrast to many similar measurements with HSA and other proteins and different ***-resolved PL data revealed a characteristic quenching behavior depending on the QD surface coverage with *** together,we found that MUTAB-QDs initially bind HSA molecules weakly(KD≈700μM);however,the affinity is enhanced over time,presumably due to proton injection into the MUTAB layer by HSA triggering ligand *** process was also observed with human blood serum,showing equal kinetics for comparable HSA ***,imaging experiments with cultured human cells(HeLa)revealed that MUTAB-QDs bind to the cell membrane and perforate *** process is reduced upon pre-adsorption of proteins on the MUTAB-QD.

关键词： Quantum dots Nanoparticles Agglomeration Protein adsorption Quantitative fluorescence microscopy

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Self-assembled thermosensitive luminescent nanoparticles with peptide-Au conjugates for cellular imaging and drug delivery

引用

Chinese Chemical Letters 2020年第3期31卷 859-864页

作者： Xiaoyuan Zhang Wei Liu Haixia Wang Xinne Zhao Zhenfang Zhang gerd ulrich nienhaus Li Shang Zhiqiang Su State Key Laboratory of Chemical Resource Engineering Beijing Key Laboratory of Advanced Functional Polymer CompositesBeijing University of Chemical TechnologyBeijing 100029China Faculty of Physics and Astronomy Friedrich Schiller University JenaJena 07743Germany Institute of Applied Physics Karlsruhe Institute of Technology(KIT)Karlsruhe 76128Germany Institute of Nanotechnology Karlsruhe Institute of Technology(KIT)Eggenstein-Leopoldshafen 76344Germany e Institute of Toxicology and Genetics Karlsruhe Institute of Technology(KIT)Eggenstein-Leopoldshafen 76344Germany Department of Physics University of Illinois at Urbana-ChampaignUrbanaIL 6J801 United States State Key Laboratory of Solidification Processing Center for Nano Energy MaterialsSchool of Materials Science and EngineeringNorthwestern Polytechnical UniversityShaanxi Joint Laboratory of Graphene(NPU)Xi'an 710072China

A facile and efficient strategy has been developed to fabricate a multifunctional,theranostic anticancer drug delivery platform featuring active targeting,controlled drug release and fluorescence imaging for real-time control of *** this end,thermo sensitive poly(N-isopropyl acrylamide)(PNIPAM)nanospheres are decorated with peptide-Au cluster conjugates as a smart nanomedicine platform.A sophisticated trifunctional peptide is designed to release the anticancer drug doxorubicin(DOX),target cells and reduce Au^3+ions to form luminescent Au cluste ***,the peptide-Au cluster moieties are attached to the PNIPAM nanospheres via amide bonds rather than noncovalent interactions,significantly improving their stability in biological medium and drug release *** in vitro experiments showed that DOX was released in an efficient and controlled manner under physiological conditions.

关键词： Peptide-Au bioconjugates PNIPAM nanoparticles Fluorescence Drug delivery Cell imaging

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：