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Abnormal Expression of Eukaryotic Translation Factors in Malignant Transformed Human Bronchial Epithelial Cells Induced by Crystalline Nickel Sulfide

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Biomedical and Environmental Sciences 2006年第1期19卷 53-60页

作者： YI-XIONG LEI XUE-MIN CHEN gen-rong wo JIA-KUN CHEN Department of Occupational and Environmental Health Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 Hubei China Department of Preventive Medicine Guangzhou Medical College Guangzhou 510182 Guangdong China

Objective To study the oncogenic potential of mouse translation initiation factor 3 （TIF3） and elongation factor-1δ （TEF-1δ） in malignant transformed human bronchial epithelial cells induced by crystalline nickel sulfide （NiS）. Methods Abnormal expressions of human TIF3 and TEF-1δ genes in two kinds of NiS-transformed cells and NiS-tumorigenic cell lines were investigated and analyzed by the reverse transcript polymerase chain reaction （RT-PCR） and fluorescent quantitative polymerase chain reaction （FQ-PCR）, respectively. Results RT-PCR analysis primarily showed that both human TIF3 and TEF-1δ mRNA expressions in two kinds of NiS-transformed cells and NiS-tumorigenic cell lines were increased as compared with controls. FQ-PCR assay showed that the levels of TIF3 expressions in the transformed cells and tumorigenic cells were 3 and 4 times higher respectively, and the elevated expressions of TEF-16 eDNA copies were 2.7- to 3.5-fold in transformed cells and 4.1- to 5.2-fold in tumorigenic cells when compared with non-transformed cells, indicating that the over-expressions of human TIF3 and TEF-1δ genes were related to malignant degree of the cells induced by nickel. Conclusions These findings demonstrate that there are markedly abnormal expressions of TIF3 and TEF-1δ genes during malignant transformation of human bronchial epithelial cell lines induced by crystalline NiS. They seem to be the molecular mechanisms potentially responsible for human carcinogensis due to nickel.

关键词： Nickel compounds Human cells Transformation Translation factors Fluorescent quantitative PCR

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Constructing 2D bismuth-based heterostructure for highly efficient photocatalytic CO2 reduction

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Tungsten 2025年第1期7卷 71-79页

作者： Ruo-Hang Xu Hao-Yu Jiang Dan-Dan Cui Kun-rong Du Hong-Run Zhang Sheng-Jie Xu Ya-Qi Li Zhi-Zhen Ren Liang Wang Wei-Chang Hao Yi Du School of Physics and BUAA-UOW Joint Research Centre Beihang University Shenzhen Institute of Beihang University Centre for Catalysis and Clean Energy School of Environment and Science Griffith University

Reforming carbon dioxide（CO2） into fuels and valuable chemicals using solar energy holds significant potential for emission reduction and addressing energy supply *** recombination of photocarriers remains a major obstacle,however,to enhancing solar energy conversion efficiency in the photocatalytic reaction *** two-dimensional materials,constructing heterogeneous structures has emerged as an effective strategy to enhance the separation of photoexcited carriers by leveraging the internal electric field at the heterogeneous ***,we present a facile strategy for preparing Bi4NbO8Br/Bi2wo6plate-on-plate Z-scheme junctions with a strong internal electric field by in situ *** unique heterostructure facilitates efficient separation and transport of photoexcited charges by harnessing the internal electric field induced by chemical bonding at the lateral ***,the Bi4NbO8Br/Bi2wo6heterostructure exhibits enhanced CO2photoreduction properties,along with a CO yield rate of approximately 35 μmol·g-1·h-1under full light *** work offers novel insights into the synthesis of unique hetero structures intended to enhance the efficiency of photocatalytic CO2reduction.

关键词： Bi4NbO8Br/Bi2wo6 CO2 reduction Heterostructures Photocatalysts

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Theoretical Investigations on the Structural and Electronic Properties of wo3 Polymorphs

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Chinese Journal of Structural Chemistry 2018年第12期37卷 1878-1890,1843页

作者： JIN Hua ZHOU He-gen HANG Yong-Fan College of Chemistry and Biology Engineering Yichun UniversityYichun 336000China Engineering Center of Jiangxi University for Lithium Energy Yichun UniversityYichun 336000China College of Chemistry Fuzhou UniversityFuzhou 350116China State Key Laboratory of Photocatalysis on Energy and Environment Fuzhou 350002China

The structural characteristic and electronic properties of seven wo_3 bulk polymorphs have been studied by first-principles density functional theory(DFT) calculations. Our calculation results indicate that according to the energy of wo_3 per unit, the stability of different wo_3 systems reduces in turn in the following order: monoclinic wo_3, orthorhombic wo_3, tetragonal wo_3, triclinic wo_3, hexagonal wo_3 and cubic wo_3. And when the unit cell is distorted from the cubic framework, the alternatively long and short W–O bonds are formed, implying an improvement of bonding-antibonding splitting associated with the interactions between W and O atoms. The deformation of the wo6 octahedron enhances the stability of the system to some extent though the energy difference between various wo_3 phases is small(wo_3 unit). Besides, the band gap tends to enlarge accompanied with the symmetry decrease of wo_3 bulk. According to the band structures, the minimum band gaps for those wo_3 polymorphs are direct at the Γ point except for the simple cubic and hexagonal structures. In addition, for all the wo_3 phases, the compositions of the valance band maximum(VBM) and conduction band minimum(CBM) are the same, which are relative to the O 2 p and W 5 d orbitals, respectively.

关键词： tungsten trioxide band structure electronic properties DFT

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Decentralized robust guaranteed cost control for a class of interconnected singular large-scale systems with time-delay and parameter uncertainty

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Journal of Systems Engineering and Electronics 2007年第1期18卷 111-119页

作者： wo Songlin Shi Guodong Zou Yun Division of Public Teaching Changzhou Coll. of Information Technology Changzhou 213164 P. R. China Dept. of Automation Nanjing Univ. of Science and Technology Nanjing 210094 P. R. China

The decentralized robust guaranteed cost control problem is studied for a class of interconnected singular large-scale systems with time-delay and norm-bounded time-invariant parameter uncertainty under a given quadratic cost performance function. The problem that is addressed in this study is to design a decentralized robust guaranteed cost state feedback controller such that the closed-loop system is not only regular, impulse-free and stable, but also guarantees an adequate level of performance for all admissible uncertainties. A sufficient condition for the existence of the decentralized robust guaranteed cost state feedback controllers is proposed in terms of a linear matrix inequality （LMI） via LMI approach. When this condition is feasible, the desired state feedback decentralized robust guaranteed cost controller gain matrices can be obtained. Finally, an illustrative example is provided to demonstrate the effectiveness of the proposed approach.

关键词： Uncertain singular large-scale systems Time-delay system Guaranteed cost control LMI

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Dechlorination mechanism of 2,4-dichlorophenol by Ni/Fe nanoparticles in the presence of humic acid

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2009年第1期10卷 121-126页

作者： Jing-jing wo Zhen ZHANG Xin-hua XU Department of Environmental Engineering Zhejiang University Hangzhou 310027 China School of Bioscience Taizhou University Linhai 317000 China

To understand the feasibility of its application to the in situ remediation of contaminated groundwater,the dechlori-nation of 2,4-dichlorophenol (2,4-DCP) by Ni/Fe nanoparticles in the presence of humic acid (HA) was *** found that,as high performance liquid chromatography (HPLC) was used,the 2,4-DCP was first quickly reduced to o-chlorophenol (o-CP) and p-chlorophenol (p-CP),and then reduced to phenol as the final *** experimental results indicated that HA had an adverse effect on the dechlorination of 2,4-DCP by Ni/Fe nanoparticles,as the HA concentration increased,the removal rate decreased *** also demonstrated that 2,4-DCP was reduced more easily to o-CP than to p-CP,and that the sequence of the tendency in dechlorination of intermediates was p-CP>*** electron microscope (TEM) showed that HA could act as an adsorbate to compete reactive sites on the surface of Ni/Fe nanoparticles to decrease the dechlorination *** we con-cluded that the dechlorination reaction of 2,4-DCP over Ni/Fe nanoparticles progressed through catalytic reductive dechlorination.

关键词： Dechlorination Ni/Fe nanoparticles 2 4-dichlorophenol (2 4-DCP) Humic acid (HA)

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Preliminary assessment of growth and survival of green alder(Alnus viridis), a potential biological stabilizer on fly ash disposal sites

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Journal of Forestry Research 2015年第1期26卷 131-136页

作者： Marcin Pietrzykowski wojciech Krzaklewski Bartomiej wo Department of Forest Ecology Forest FacultyUniversity of Agriculture in Krakow

This paper presents preliminary assessment of seedling survival and growth of green alder（Alnus viridis（Chaix） DC. in Lam. ＆ DC.） planted on fly ash disposal sites. This kind of post-industrial site is extremely hard to biologically stabilize without top-soiling. The experiment started with surface preparation using NPK start-up mineral fertilizer at 60–36–36 kg ha-1followed by initial stabilization through hydro-seeding with biosolids（sewage sludge 4 Mg ha-1dry mass） and a mixture of grasses（Dactylis glomerata L. and Lolium multiflorum Lam.）（200 kg ha-1）. Subsequently, three-years-old green alder seedlings were planted in plots on two substrate variants：the control（directly on combustion waste） and plots with3 dm3 lignite culm from a nearby mine introduced into the planting pit. Five years of preliminary monitoring show good survival seedling rates and growth parameters（height（h）, average increase in height（Dh）, number of shoots（L o）and leaf nitrogen supply in the fly ash disposal *** of the site with a combination of lignite culm in planting pits and preliminary surface preparation by hydroseeding and mineral fertilization had the most positive effect on green alder seedling parameters. The results indicate that it is possible and beneficial to use green alder for biological stabilization on fly ash disposal sites.

关键词： Fly ash Green alder Seedlings survival Growth Biological stabilisation

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OsGLU3, a Putative Membrane-Bound Endo-1, 4-Beta-Glucanase, Is Required for Root Cell Elongation and Division in Rice （Oryza sativa L.）

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Molecular Plant 2012年第1期5卷 176-186页

作者： Jin-Wei Zhang Lei XU Yun-rong Wu Xin-Ai Chen Yu Liu Shi-Hua Zhu wo-Na Ding Ping Wu Ke-Ke Yi The State Key Laboratory of Plant Physiology and Biochemistry College of Life ScienceZhejiang UniversityHangzhou 310058China College of Science and Technology Ningbo UniversityNingboZhejiang 315211China""'1 State key Laboratory Breeding Base forZhejiang Sustainable Pest and Disease Control Institute of Virology and BiotechnologyZhejiang Academy of Agricultural SciencesHangzhou 310021China

Plant roots move through the soil by elongation. This is vital to their ability to anchor the plant and acquire water and minerals from the soil. In order to identify new genes involved in root elongation in rice, we screened an ethyl methane sulfonate （EMS）-mutagenized rice library, and isolated a short root mutant, Osglu3-1. The map-based cloning results showed that the mutant was due to a point mutation in OsGLU3, which encodes a putative membrane-bound endo- 1,4-13-glucanase. Osglu3-1 displayed less crystalline cellulose content in its root cell wall, shorter root cell length, and a slightly smaller root meristem as visualized by restricted expression of OsCYCBI, I：GUS. Exogenous application of glu- cose can suppress both the lower root cell wall cellulose content and short root phenotypes of Osglu3-1. Consistently, OsGLU3 is ubiquitously expressed in various tissues with strong expression in root tip, lateral root, and crown root pri- modia. The fully functional OsGLU3-GFP was detected in plasma membrane, and FM4-64-1abeled compartments in the root meristem and elongation zones. We also found that phosphate starvation, an environmental stress, altered cell wall cel- lulose content to modulate root elongation in a OsGLU3-dependant way.

关键词： Abiotic/environmental stress nutrition cell expansion root biology.

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Binary neutron stars gravitational wave detection based on wavelet packet analysis and convolutional neural networks

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Frontiers of physics 2020年第2期15卷 111-117页

作者： Bai-Jiong Lin Xiang-Ru Li wo-Liang Yu South China Normal University Guangzhou 510631China

This work investigates the detection of binary neutron stars gravitational wave based on convolutional neural network(CNN).To promote the detection performance and efficiency,we proposed a scheme based on wavelet packet(WP)decomposition and *** WP decomposition is a time-frequency method and can enhance the discriminant features between gravitational wave signal and noise before *** CNN conducts the gravitational wave detection by learning a function mapping relation from the data under being processed to the space of detection *** function-mapping-relation style detection scheme can detection efficiency *** this work,instrument effects are con-sidered,and the noise are computed from a power spectral density(PSD)equivalent to the Advanced LIGO design *** quantitative evaluations and comparisons with the state-of-art method matched filtering show the excellent performances for BNS gravitational wave *** efficiency,the current experiments show that this WP-CNN-based scheme is more than 960 times faster than the matched filtering.

关键词： gravitational waves algorithms astrostatistics techniques

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Kinetics of 2,4-dichlorophenol dechlorination by Pd-Fe bimetallic nanoparticles in the presence of humic acid

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2008年第1期9卷 118-124页

作者： Zhen ZHANG Jing-jing wo Naman CISSOKO Xin-hua XU Department of Environmental Engineering Zhejiang University Hangzhou 310027 China School of Bioscience Taizhou University Linhai 317000 China

The remediation of groundwater which contains chlorinated organic compounds (COCs) by nanoscale bimetallic catalysts has received increasing interest in recent years. This report presents the dechlorination of 2,4-dichlorophenol (2,4-DCP) by Pd-Fe bimetallic nanoparticles in the presence of humic acid (HA) to investigate the feasibility of using Pd-Fe for the in situ remediation of contaminated groundwater. Our experimental results indicated that HA had an adverse effect on the dechlorination of 2,4-DCP by Pd-Fe nanoparticles. The rate constant k values of 2,4-DCP dechlorination were 0.017, 0.013, 0.009, 0.006 and 0.004 min?1 for HA concentrations of 0, 5, 10, 15 and 20 mg/L, respectively. The relationship between HA dosage and k values can be described as a linear model.

关键词： 2,4-dichlorophenol (2,4-DCP) Dechlorination Pd-Fe bimetallic nanoparticles Humic acid (HA)

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Phylogenetic,Demographic and Dating Analyses of Bufo gargarizans Populations from the Zhoushan Archipelago and China's Mainland

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Asian Herpetological Research 2017年第3期18卷 165-173页

作者： Haojie TONG Yubin wo Pinghu LIAO Yuanting JIN College of Life Sciences China Jiliang University

Relatively little is known about the relationship between Bufo gargarizans populations from Zhoushan Archipelago and nearby continental regions on the Pacific coast of eastern *** this paper,155 new specimens of *** from Zhoushan Archipelago and adjacent continents and 71 published specimens of *** from China's Mainland were *** and dating analyses of *** were performed using mitochondrial DNA sequencing with a length of 1436 bp.A mt DNA tree that indicated seven major clades was *** earliest split in the mt DNA tree corresponding to the divergence of populations from the western highland region occurred approximately 4.0 million years ago（mya）.A subsequent clade occurred about 3.4 mya,with cladogenesis continuing toward the end of the *** continental clades were distributed in the western,central and northeastern regions of *** Archipelago clades consisted of two largely geographically overlapping subclades with the mt DNA divergence time of 0.73 *** results indicated there was extensive dispersal after *** *** populations on Zhoushan Archipelago most probably originated from populations in nearby eastern continental regions of *** was concluded that geological uplifting during the Pliocene and several sea-level changes in Pleistocene might have influenced the divergence and population demographical history of this species.

关键词： genetic structure island phylogeography mitochondrial DNA Bufo gargarizans

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