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临床检验杂志 2013年第5期31卷 397-400页

作者： robert H.Christenson gary L.Myers Gerald r.Cooper 徐国宾李江郑芳鄢盛恺

对b型钠尿肽（bNP）和N末端b型钠尿肽前体（NT-probNP）的建议根据对文献综述,提出bNP和NT-probNP在CVD和卒中一级预防危险评估时的临床应用和检测建议。

关键词： b型钠尿肽 N末端 NT-probNP 前体心血管病危文献综述临床应用

二维分离在一日内实现:二维凝胶电泳工作流程在五小时内完成

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生命科学仪器 2010年第5期8卷 22-25页

作者： gary b. SMEJKAL Darren J. bAUEr the Hubbard Center for Genome Studies University of New Hampshire Durham NH 03824 U.S.A.

介绍了一种微处理器控制等电聚焦(IEF) Hoefer IEF100系统,其能够施加12 000 V电压,使得蛋白质的二维电泳(2DE)的第一维能够在3 h内完成。当其在Hoefer SE260小型垂直电泳槽中与作为第二维的聚丙烯酰胺凝胶电泳(PAGE)联用时,那么整个二... 详细信息

介绍了一种微处理器控制等电聚焦(IEF) Hoefer IEF100系统,其能够施加12 000 V电压,使得蛋白质的二维电泳(2DE)的第一维能够在3 h内完成。当其在Hoefer SE260小型垂直电泳槽中与作为第二维的聚丙烯酰胺凝胶电泳(PAGE)联用时,那么整个二维的工作流程能够在5 h内完成。

关键词：二维凝胶电泳工作流程二维分离聚丙烯酰胺凝胶电泳小时微处理器控制等电聚焦二维电泳

Control of bone Mass by Novel runx2 regulatory Pathways

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Control of Bone Mass by Novel Runx2 Regulatory Pathways

第十届国际骨矿研究学术会议暨第十二届国际骨质疏松研讨会

作者： Jane b.Lian gary S.Stein Andre J.van Wijnen Janet L.Stein Department of Cell biology University of Massachusetts Medical School

＜正＞Osteoporosis is a multifactorial disease with many contributing factors leading to bone loss caused by an age-related decrease in the number and activity of osteoblasts and further exacerbated by hormonal changes leading to increased osteoclast bone *** discovery of the master transcription factor,runx2 that is essential for commitment and differentiation of mesenchymal stem cells（MSCs）to osteoblasts and bone

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利用双电极装置提高等电聚焦通量

生命科学仪器 2011年第6期9卷 28-29页

作者： gary b.SMEJKAL Darren J.bAUEr the Hubbard Center for Genome Studies University of New Hampshire

在最大电压12 000 V下操作,蛋白质在固定pH梯度(IPGs)上的等电聚集(IEF)可以在3 h内完成。采用改装的电极装置,将两电极对通过线路并联,可使IEF的通量加倍。、与常规的单电极对相比,成对的IPGs的电压梯度几乎一致,并在大致两倍的电流下... 详细信息

在最大电压12 000 V下操作,蛋白质在固定pH梯度(IPGs)上的等电聚集(IEF)可以在3 h内完成。采用改装的电极装置,将两电极对通过线路并联,可使IEF的通量加倍。、与常规的单电极对相比,成对的IPGs的电压梯度几乎一致,并在大致两倍的电流下运行,IEF可在大致相同的时间内完成。

关键词：双电极等电聚焦通量装置利用电压梯度 pH梯度电极对

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结构改革与中国工业增长

经济研究 2009年第7期44卷 4-20页

作者：张军陈诗一 gary H. Jefferson 复旦大学中国社会主义市场经济研究中心 200433 美国布兰迪斯大学(brandeis University)

1978年以来,中国工业在持续的结构改革中经历了强劲的增长和生产率水平的不断提高。本文估算了工业分行业随机前沿生产函数,发现1992年后,TFP增长超过了要素投入增长,但是TFP对工业增长的贡献在2001年后出现了下降。进一步对TFP增长分... 详细信息

1978年以来,中国工业在持续的结构改革中经历了强劲的增长和生产率水平的不断提高。本文估算了工业分行业随机前沿生产函数,发现1992年后,TFP增长超过了要素投入增长,但是TFP对工业增长的贡献在2001年后出现了下降。进一步对TFP增长分解后发现,由工业结构改革引致的行业间要素重置显然对改革开放期间中国工业生产率的提高乃至工业增长起到了实际推动作用,即结构红利是显著存在的,而2001年后要素配置效率的下降也成为同期TFP增长贡献份额下降的主要原因。因素回归分析显示,中国要素市场的改革和工业行业的结构调整主导了要素配置效率变化的总体走势,并造成了不同行业要素配置效率的显著差异。

关键词：结构改革要素配置效率结构红利生产率变化工业增长

Cartilage oligomeric matrix protein: A novel non-invasive marker for assessing cirrhosis and risk of hepatocellular carcinoma

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World Journal of Hepatology 2015年第14期7卷 1875-1883页

作者： gary L Norman Nikolaos K Gatselis Zakera Shums Christos Liaskos Dimitrios P bogdanos George K Koukoulis George N Dalekos Inova Diagnostics Inc. Department of Medicine and research Laboratory of Internal Medicine School of MedicineUniversity of Thessaly Division of rheumatology School of MedicineUniversity of Thessaly Division of Transplantation Immunology and Mucosal biology Institute of Liver StudiesKing’s College London School of MedicineDenmark Hill Campus Department of Pathology School of MedicineUniversity of Thessaly

AIM: To assess serum cartilage oligomeric matrix protein(COMP) as a marker of cirrhosis and risk of progression to hepatocellular carcinoma(HCC). METHODS: A COMP enzyme-linked immunosorbentassay was used to test 187 patients with chronic liver diseases at the time point of first evaluation. The selected patients included 72 with chronic hepatitis b infection, 75 with chronic hepatitis C infection, 22 with primary biliary cirrhosis, 7 with autoimmune hepatitis type 1, and 11 with alcoholic liver disease. Demographic, biochemical, histological and clinical characteristics of the patients were recorded at the first evaluation. One hundred and forty-seven patients were followed for a median [interquartile range(IQr)] duration of 96.5(102) mo. The clinical, biochemical and histological data, as well as the development of cirrhosis, HCC according to internationally accepted criteria and in case of death, a liver-related cause during the follow-up period, were recorded at the electronic database of our clinic. COMP determination was also performed in 43 healthy individuals who served as the control study ***: COMP positivity(> 15 U/L) was detected in 22%-36% among chronic liver disease groups. Strikingly, almost 83% of COMP-positive patients were cirrhotic at baseline, independently of cause of liver disease. Among the patients who developed HCC during follow-up, 73.7%(14/19) were COMP positive at baseline. COMP positivity was significantly associated with older age(P brosis(P = 0.001) and necroinflammatory activity(P = 0.001), higher aspartate aminotransferase(P ransferase(P ranspeptidase(P = 0.003), alkaline phosphatase(P = 0.001), bilirubin(P rnational normalized ratio(P = 0.002) and alpha-fetoprotein levels(P r albumin(P r)] were significantly higher in cirrhotics compared to non-cirrhotics [13.8(7.9) U

关键词： Hepatic fibrosis Hepatocellular carcinoma Viral hepatitis Enzyme-linked immunosorbent assay biomarker Cirrhosis

美国临床生化科学院检验医学实践指南:心血管疾病和卒中一级预防中新的生物标志物第6章载脂蛋白AⅠ和载脂蛋白b与心血管疾病危险

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临床检验杂志 2013年第5期31卷 382-387页

作者： gary L.Myers Peter W.F.Wilson Gerald r.Cooper Scott M.Grundy Darwin r.Labarthe 王永志郑芳鄢盛恺

对载脂蛋白AⅠ与载脂蛋白b的建议根据对文献的综述,提出以下载脂蛋白AⅠ（apo AⅠ）和载脂蛋白b（apo b）在CHD和卒中一级预防危险评估中的临床应用及测定建议。

关键词：载脂蛋白AⅠ 载脂蛋白b 心血管病危临床应用危险评估一级预防 CHD

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舌尖上的大脑

利用HLA-Drβ1亚型同源多肽筛选类风湿关节炎相关抗原的研究

作者： (美)gary L.Wenk

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中国免疫学杂志 2002年第3期18卷 152-154页

作者：栗占国肖利群韩蕾 gary Pestano 北京大学人民医院风湿免疫科北京100034 美国哈佛大学医学院Dana Farber研究所免疫实验室

目的 :筛选与类风湿关节炎易感基因HLA Drβ1亚型同源多肽结合的抗原性蛋白。方法 :利用HLA Drβ1亚型同源多肽亲和层析柱结合类风湿关节炎患者b细胞可溶性蛋白 ,并以pH梯度洗脱获取HLA Drβ1同源多肽结合蛋白。结果 :类风湿关节炎易... 详细信息

目的 :筛选与类风湿关节炎易感基因HLA Drβ1亚型同源多肽结合的抗原性蛋白。方法 :利用HLA Drβ1亚型同源多肽亲和层析柱结合类风湿关节炎患者b细胞可溶性蛋白 ,并以pH梯度洗脱获取HLA Drβ1同源多肽结合蛋白。结果 :类风湿关节炎易感基因HLA Drβ1亚型同源多肽可高亲和力结合II型胶原、热休克蛋白 90和组蛋白等 5种抗原蛋白。结论 :II型胶原及热休克蛋白 90可能通过与HLA Drβ1分子形成二聚体复合物参与类风湿关节炎的发病。

关键词：类风湿关节炎 HLA-Drβ1 亚型结合抗原同源多肽 PH梯度洗脱 Ⅱ型胶原热休克蛋白

Golgi protein-73:A biomarker for assessing cirrhosis and prognosis of liver disease patients

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World Journal of Gastroenterology 2020年第34期26卷 5130-5145页

作者： Nikolaos K Gatselis Tamás Tornai Zakera Shums Kalliopi Zachou Asterios Saitis Stella Gabeta roger Albesa gary L Norman Mária Papp George N Dalekos Department of Medicine and research Laboratory of Internal Medicine National Expertise Center of Greece in Autoimmune Liver DiseasesGeneral University Hospital of LarissaLarissa 41110Greece Institute of Internal Medicine and Hepatology Larissa 41447Greece Department of Internal Medicine Division of GastroenterologyUniversity of DebrecenFaculty of MedicineDebrecen H-4032Hungary Department of research and Development Inova DiagnosticsInc.San DiegoCA 92131United States

bACKGrOUND reliable biomarkers of cirrhosis,hepatocellular carcinoma(HCC),or progression of chronic liver diseases are *** this context,Golgi protein-73(GP73)also called Golgi phosphoprotein-2,was originally defined as a resident Golgi type II transmembrane protein expressed in epithelial *** a result,GP73 expression was found primarily in biliary epithelial cells,with only slight detection in ***,in patients with acute or chronic liver diseases and especially in HCC,the expression of GP73 is significantly up-regulated in *** far,few studies have assessed GP73 as a diagnostic or prognostic marker of liver fibrosis and disease *** To assess serum GP73 efficacy as a diagnostic marker of cirrhosis and/or HCC or as predictor of liver disease *** GP73 serum levels were retrospectively determined by a novel GP73 ELISA(QUANTA Lite®GP73,Inova Diagnostics,Inc.,research Use Only)in a large cohort of 632 consecutive patients with chronic viral and non-viral liver diseases collected from two tertiary Academic centers in Larissa,Greece(n=366)and Debrecen,Hungary(n=266).Aspartate aminotransferase(AST)/Platelets(PLT)ratio index(APrI)was also calculated at the relevant time points in all *** hundred and three patients had chronic hepatitis b,183 chronic hepatitis C,198 alcoholic liver disease,28 autoimmune cholestatic liver diseases,15 autoimmune hepatitis,and 5 with other liver-related *** duration of follow-up was 50(57)mo[median(interquartile range)].The development of cirrhosis,liver decompensation and/or HCC during follow-up were assessed according to internationally accepted *** particular,the surveillance for the development of HCC was performed regularly with ultrasound imaging and alpha-fetoprotein(AFP)determination every 6 mo in cirrhotic and every 12 mo in non-cirrhotic *** Increased serum levels of GP73(>20 units)were detected at initial evaluation in 277 out of

关键词： biomarker Golgi protein-73 Hepatic fibrosis Cirrhosis Hepatocellular carcinoma Hepatitis b Hepatitis C Aspartate aminotransferase/Platelets ratio index score