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先进生物材料在创面修复中的应用

中华烧伤与创面修复杂志 2024年第1期40卷 26-32页

作者：罗高兴周璇陆军军医大学(第三军医大学)第一附属医院全军烧伤研究所创伤与化学中毒全国重点实验室重庆市创面损伤修复与再生重点实验室重庆400038

皮肤创面愈合是一个极其复杂的病理生理过程。随着材料科学、组织工程学等快速发展,通过多学科融合研发的先进生物材料具有更多功能与作用,为加快创面修复速度、改善创面修复质量,甚至组织再生提供了新的思路、策略与方法。该文重点阐... 详细信息

皮肤创面愈合是一个极其复杂的病理生理过程。随着材料科学、组织工程学等快速发展,通过多学科融合研发的先进生物材料具有更多功能与作用,为加快创面修复速度、改善创面修复质量,甚至组织再生提供了新的思路、策略与方法。该文重点阐述了不同种类的多功能先进生物材料在促进创面修复中的应用。

关键词：皮肤生物相容性材料组织工程创面愈合先进生物材料多功能

A ROS-responsive,self-immolative and self-reporting hydrogen sulfide donor with multiple biological activities for the treatment of myocardial infarction

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Bioactive Materials 2022年第3期7卷 168-182页

作者： Mengyun Yao Yifei Lu Lin Shi Yong Huang Qing Zhang Jianglin Tan Ping Hu Jianxiang Zhang gaoxing luo Ning Zhang Institute of Burn Research Southwest HospitalState Key Lab of TraumaBurn and Combined InjuryChongqing Key Laboratory for Disease ProteomicsThird Military Medical University(Army Medical University)Chongqing 400038China College of Pharmacy Jinan UniversityGuangzhou 511443China Department of Pharmaceutics College of PharmacyThird Military Medical University(Army Medical University)Chongqing 400038China

Myocardial infarction(MI),as one of the leading causes of global death,urgently needs effective therapies.Recently,hydrogen sulfide(H2S)has been regarded as a promising therapeutic agent for MI,while its spatiotemporally controlled delivery remains a major issue limiting clinical translation.To address this limitation,we designed and synthesized a novel H2S donor(HSD-R)that can produce H2S and emit fluorescence in response to reactive oxygen species(ROS)highly expressed at diseased sites.HSD-R can specifically target mitochondria and provide red fluorescence to visualize and quantify H2S release in vitro and in vivo.Therapeutically,HSD-R significantly promoted the reconstruction of cardiac structure and function in a rat MI model.Mechanistically,myocardial protection is achieved by reducing cardiomyocyte apoptosis,attenuating local inflammation,and promoting angiogenesis.Furthermore,inhibition of typical pro-apoptotic genes(Bid,Apaf-1,and p53)played an important role in the anti-apoptotic effect of HSD-R to achieve cardioprotection,which were identified as new therapeutic targets of H2S against myocardial ischemia injury.This ROS-responsive,self-immolative,and fluorescent H2S donor can serve as a new theranostic agent for MI and other ischemic diseases.

关键词： Hydrogen sulfide donor Reactive oxygen species Myocardial infarction Molecular imaging Theranostic agent

iTRAQ-based proteomic profiling reveals different protein expression between normal skin and hypertrophic scar tissue

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Burns & Trauma 2015年第2期3卷 106-113页

作者： Jianglin Tan Weifeng He gaoxing luo Jun Wu Institute of Burn Research State Key Laboratory of TraumaBurns and Combined InjuriesChongqing Key Laboratory for Disease ProteomicsSouthwest HospitalThird Military Medical UniversityChongqing 400038China

Background:A hypertrophic scar is a unique fibrotic disease that only exists in humans.Despite advances in burn care and rehabilitation,as well as progress in the management during these decades,the hypertrophic scar remains hard to cure following surgical methods and drugs for treatment.In this study,we are looking forward to finding the multitude of possible traumatic mechanisms and the underlying molecular signal ways in the formation of the hypertrophic scar.Methods:We used isobaric tags for relative and absolute quantitation(iTRAQ)labeling technology,followed by high-throughput 2D LC-MS/MS,to determine relative quantitative differential proteins between the hypertrophic scar and normal skin tissue.Results:A total of 3166 proteins were identified with a high confidence(≥95%confidence).And,a total of 89 proteins were identified as the differential proteins between the hypertrophic scar and normal skin,among which 41 proteins were up-regulated and 48 proteins were down-regulated in the hypertrophic scar.GO-Analysis indicated the up-regulated proteins were involved in extracellular matrix,whereas the down-regulated proteins were involved in dynamic junction and structural molecule activity.Conclusions:In our study,we demonstrate 89 proteins present differently in the hypertrophic scar compared to normal skin by iTRAQ technology,which might indicate the pathologic process of hypertrophic scar formation and guide us to propose new strategies against the hypertrophic scar.

关键词： iTRAQ Hypertrophic scar Proteomics

Extracorporeal membrane oxygenation combined with continuous renal replacement therapy for the treatment of severe burns:current status and challenges

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Burns & Trauma 2021年第1期9卷 401-408页

作者： Huapei Song Zhiqiang Yuan Yizhi Peng gaoxing luo State Key Laboratory of Trauma Burn and Combined InjuryInstitute of Burn ResearchSouthwest HospitalThird Military Medical University(Army Medical University)30 Gaotanyan StreetChongqing 400038China

Severe burns often cause various systemic complications and multiple organ dysfunction syndrome,which is the main cause of death.The lungs and kidneys are vulnerable organs in patients with multiple organ dysfunction syndrome after burns.Extracorporeal membrane oxygenation(ECMO)and continuous renal replacement therapy(CRRT)have been gradually applied in clinical practice and are beneficial for severe burn patients with refractory respiratory failure or renal dysfunction.However,the literature on ECMO combined with CRRT for the treatment of severe burns is limited.Here,we focus on the current status of ECMO combined with CRRT for the treatment of severe burns and the associated challenges,including the timing of treatment,nutrition support,heparinization and wound management,catheter-related infection and drug dosing in CRRT.With the advancement of medical technology,ECMO combined with CRRT will be further optimized to improve the outcomes of patients with severe burns.

关键词： Severe burns Extracorporeal membrane oxygenation Continuous renal replacement therapy Multiple organ dysfunction syndrome Acute kidney injury Acute respiratory distress syndrome

Mixed lymphocyte reaction induced by multiple alloantigens and the role for IL-10 in proliferation inhibition

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Burns & Trauma 2014年第1期2卷 24-28页

作者： Junyi Zhou Weifeng He gaoxing luo Jun Wu State Key Laboratory of Trauma Burn and Combined InjuryInstitute of Burn ResearchSouthwest Hospitalthe Third Military Medical University Chongqing Key Laboratory for Proteomics of Diseases ChongqingChina

The frequency of T cells that can respond to alloantigens is unusually high.It remains unclear how T cells would respond when stimulated by multiple major histocompatibility complex(MHC)disparate alloantigens in the same cultures.In this report,we examined potential interactions of T cell clones that were stimulated simultaneously by two sets of complete MHC disparate alloantigens using mixed lymphocyte reaction(MLR).In this assay,we observed that proliferation of B6 lymphocytes(H-2b)stimulated by both BALB/c(H-2d)and C_(3)H(H-2k)allogeneic cells was not increased but rather reduced as compared to B6 cells stimulated with either BALB/c or C_(3)H allogeneic cells.Interestingly,interleukin(IL)-10 expressions at both protein level and mRNA level was signifi cantly increased in cultures stimulated with the two MHC alloantigens,while IL-2,tumor necrosis factor(TNF)-α,transforming growth factor(TGF)-β1 production did not show any differences.In addition,Foxp_(3) mRNA expression was comparable amongst all groups.In conclusion,we observed an inhibitory effect in T cell proliferation in response to multiple MHC mismatched alloantigens in MLR,and this effect might be associated with the upregulation of IL-10 expression.

关键词： Interleukin-10 T-lymphocytes intraclonal competition mixed lymphocyte reaction

Comparative proteomic analysis of extracellular matrix proteins secreted by hypertrophic scar witb normal skin fibroblasts

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Burns & Trauma 2014年第2期2卷 76-83页

作者： Li Ma Chengjun Gan Yong Huang Ying Wang gaoxing luo Jun Wu Chongqing Key Laboratory for Disease Proteomics State Key Laboratory of Trauma Burns and Combined Injury Institute of Burn Research Southwest Hospital the Third Military Medical University Chongqing School of Environmental Air Security and Pollution Control Engineering Jinan University Guangzhou Chongqing Key Laboratory for Disease Proteomics State Key Laboratory of Trauma Burns and Combined Injury Institute of Burn Research Southwest Hospital the Third Military Medical University Chongqing The 421 Hospital People's Liberation Army Guangzhou China Chongqing Key Laboratory for Disease Proteomics State Key Laboratory of Trauma Burns and Combined Injury Institute of Burn Research Southwest Hospital the Third Military Medical University Chongqing

The formation of hypertrophic scars (HSs) is a fibroproliferative disorder of abnormal wound healing. HSs usually characterize excessive proliferation of fibroblasts, abnormal deposition of extracellular matrix (ECM) during wound healing, associated with cosmetic, functional, and psychological problems. Owing to the role of ECM proteins in scar formation, we comparatively analyzed matrix proteins secreted by normal skin fibroblasts (NSFs) and HS fibroblasts (HSFs). The acetone-extracted secreted proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and identified by mass spectrometry (MS). Based on Go annotation of MS data, the profiling of ECM proteins was established and scar-related proteins have been screened out. The functions of several ECM proteins identified by MS have been discussed, such as collagens I, VI, XII, fibronectin, decorin, lumican, and protein procollagen C endopeptidase enhancer 1 (PCPE-1). Among them, the MS result of PCPE-1 was supported by Western blotting that PCPE-1 from HSFs were significantly upregulated than that from NSFs. It is suggested that PCPE-1 could be a potential target for scar treatment. The exploration of scar related proteins may provide new perspectives on understanding the mechanism of scar formation and open a new way to scar treatment and prevention.

关键词： Extracellular matrix protein fibroblast proteomics hypertrophic scar

A biological membrane-based novel excisional wound-splinting model in mice (With video)

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Burns & Trauma 2014年第4期2卷 196-200页

作者： Zhihui Yao Yong Huang gaoxing luo Jun Wu Weifeng He State Key Laboratory of Trauma Burns and Combined InjuryInstitute of Burn ResearchSouthwest HospitalThe Third Military Medical UniversityChongqing Key Laboratory for Proteomics of DiseasesChongqingChina

Rodents have robust wound healing mechanism compared to other animal species.The major mechanisms of wound healing diff er between rodents and humans.In humans,wound healing primarily depends on re-epithelialization and granulation tissue(GT)formation,whereas wound contraction is more important during rodent wound closure.In this study,we described a novel excisional wound-splinting model in mice with a new biological membrane to imitate wound healing in humans.In this model,wound contraction can be eff ectually prevented,and the extent of re-epithelialization and the amount of granulation tissue can be determined easily.Furthermore,the harvested tissues can be analyzed with diff erent methods according to the research aim.In conclusion,we have developed a biological membrane-based,novel,excisional wound-splinting model in mice that has unique advantages for wound healing research compared with the conventional animal model.

关键词： Animal model biological membrane re-epithelialization wound healing

A systematic and quantitative method for wound-dressing evaluation

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Burns & Trauma 2015年第2期3卷 114-121页

作者： Xiaorong Zhang Rui Xu Xiaohong Hu gaoxing luo Jun Wu Weifeng He Chongqing Key Laboratory for Disease Proteomics State Key Lab of TraumaBurns and Combined InjuryInstitute of Burn ResearchSouthwest HospitalThe Third Military Medical UniversityChongqing 400038China

Background:For patients with skin defects such as burns,wound dressing plays important roles in protecting the wound.Before a novel wound dressing is applied to a patient,a series of tests should be performed to ensure its safety and efficacy.Different types of animal wound-healing models have been used to study the bio-function of different wound dressings;however,a systematic way to evaluate the effect of a wound dressing on wound healing and cutaneous regeneration is lacking.Methods:In the study presented here,full-thickness wound models were established in mice,and a systematic way to quantitatively analyze the wound-healing process and the histological results is described.Results:It was found that the rate of wound healing in the tested wound dressing(TWD)group was higher than that in the control group,and the re-epithelialization and the formation of granulation tissue were enhanced when the TWD was applied.Meanwhile,the inflammatory response was attenuated in the TWD group,and more mature and better aligned collagen fibers in the healed wound tissue was found in the TWD group compared with that in the control group.Conclusions:A systematic,quantitative way to analyze the effect of a wound dressing on wound healing was established.And it might be helpful for the design of wound dressing in the future.

关键词： Wound dressing Evaluation Wound model

Characteristics of burn deaths from 2003 to 2009 in a burn center: A retrospective study

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Burns & Trauma 2013年第2期1卷 80-86页

作者： Jian Chen Hong Yan gaoxing luo Qizhi luo Xiaolu Li Jiaping Zhang Zhiqiang Yuan Daizhi Peng Yizhi Peng Jianian Hu Jun Wu Institute of Burn Research Southwest Hospital State Key Laboratory of Trauma Burns and Combined Injury The Third Military Medical University Chongqing China

Mortality remains one of the most important end-point quality control parameters to evaluate a burn care system. We retrospectively reviewed the characteristics and multiple organ dysfunction syndrome (MODS) patterns of burn deaths in our center from January 2003 to December 2009. The mortality rate during this time period was 2.3%. Fifty-six patients died, including 49 males and 7 females. The mean survival time was 28.45 ± 24.60 days. The burn percentage was (76.70 ± 26.86) % total burn surface area (TBSA), with (27.74 ± 24.95) % deep-partial thickness burns and (46.88 ± 33.84) % full-thickness burns. Inhalation injury was diagnosed in 36 (64.29%) patients. Patients who had undergone an operation, particularly in the first week post-burn, had a significantly longer survival time. An average of 5.50 ± 1.35 malfunctioning organs per patient and a mean sequential organ failure assessment (SOFA) score of 13.91 ± 3.65 were observed. The most frequently malfunctioning organs were involved in the respiratory, hematologic, circulatory, and central nervous systems. Most of the organ damage occurred during the first week post-burn, followed by 4 weeks later, with relatively less organ damage observed in the third week. Among patients with a TBSA over 50%, non-survivors had larger burn sizes (particularly larger full-thickness burns) and a higher incidence of inhalation injury compared with survivors;nonsurvivors were also more likely to have microorganism-positive blood and sputum cultures. In conclusion, burn deaths are related to a higher burn percentage, inhalation injury, MODS, and infection. Early operation may help improve survival duration.

关键词： Burns death multiple organ dysfunction syndrome