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柔嫩艾美耳球虫裂殖生殖阶段超微结构的研究

畜牧兽医学报 2001年第5期32卷 452-457页

作者：安健汪明孔繁瑶殷佩云北京农学院动物科技系 102206 中国农业大学动物医学院北京100094

利用透射电镜对柔嫩艾美耳球虫 (Eimeriatenella)裂殖生殖阶段的超微结构进行了观察描述。其第二次裂殖生殖方式为外裂殖生殖 ,裂殖子侵入宿主细胞后 ,裂殖子逐渐变圆形成裂殖体 ,此过程中裂殖子的棒状体首先消失 ,此后锥体和极环消失... 详细信息

利用透射电镜对柔嫩艾美耳球虫 (Eimeriatenella)裂殖生殖阶段的超微结构进行了观察描述。其第二次裂殖生殖方式为外裂殖生殖 ,裂殖子侵入宿主细胞后 ,裂殖子逐渐变圆形成裂殖体 ,此过程中裂殖子的棒状体首先消失 ,此后锥体和极环消失、最后微线、淀粉颗粒和折光体消失。裂殖体长大后 ,细胞膜下陷 ,将裂殖体分成几部分 ,随后裂殖体进行核分裂形成多核裂殖体 ,此后在靠近细胞核处细胞膜加厚外突 ,临近部位的细胞膜凹陷 ,同时近突起部位内膜复合体加厚形成极环 ,随着时间的推移逐渐形成幼稚裂殖子 ,幼稚裂殖子后部与残体相连 ,此过程中裂殖子的锥体、极环、微线、棒状体、淀粉颗粒、折光体依次逐渐形成。裂殖子成熟的标志为从残体脱离 ,裂殖子的膜下微管 2 4根纵向贯穿裂殖子直达顶端和极环相连 ,裂殖子内部由锥体、锥体前环 1.锥体前环 2、极环、微线、线粒体、折光体和 3 多个棒状体等组成。

关键词：柔嫩艾美耳球虫超微结构裂殖生殖裂殖子鸡

TWAS facilitates gene-scale trait genetic dissection through gene expression,structural variations,and alternative splicing in soybean

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Plant Communications 2024年第10期5卷 16-32页

作者： Delin Li Qi wang Yu Tian Xiangguang Lyv Hao Zhang Huilong Hong Huawei {1. Yan-Fei Li Chaosen Zhao Jiajun wang Ruizhen wang Jinliang yang Bin Liu Patrick S.Schnable James C.Schnable Ying-Hui Li Li-Juan Qiu The National Key Facility for Crop Gene Resources and Genetic Improvement(NFCRI)/Key Laboratory of Crop Gene Resource and Germplasm Enhancement(MOA)/State Key Laboratory of Crop Gene Resources and Breeding Institute of Crop ScienceChinese Academy of Agricultural SciencesBeijing 100081China college of Agriculture Northeast Agricultural UniversityHarbin 150030China National Nanfan Research Institute(Sanya) Chinese Academy of Agricultural SciencesSanya 572024China Crops Research Institute of Jiangxi Academy of agricultural {1.s Nanchang 330200China Soybean Research Institute Heilongjiang Academy of Agricultural SciencesHarbin 150086China Department of Agronomy and Horticulture University of Nebraska-LincolnLincolnNE 68583USA Department of Agronomy Iowa State UniversityAmesIA 50011USA

A genome-wide association study(GWAS)identifies trait-associated loci,but identifying the causal genes can be a bottleneck,due in part to slow decay of linkage disequilibrium(LD).A transcriptome-wide association study(TWAS)addresses this issue by identifying gene expression-phenotype associations or integrating gene expression quantitative trait loci with GWAS ***,we used self-pollinated soybean(Glycine max[L.]Merr.)as a model to evaluate the application of TWAS to the genetic dissection of traits in plant species with slow LD *** generated RNA sequencing data for a soybean diversity panel and identified the genetic expression regulation of 29286 soybean *** TWAS solutions were less affected by LD and were robust to the source of expression,identifing known genes related to traits from different tissues and developmental *** novel pod-color gene L2 was identified via TWAS and functionally validated by genome *** introducing a new exon proportion feature,we significantly improved the detection of expression variations that resulted from structural variations and alternative *** a result,the genes identified through our TWAS approach exhibited a diverse range of causal variations,including SNPs,insertions or deletions,gene fusion,copy number variations,and alternative *** this approach,we identified genes associated with flowering time,including both previously known genes and novel genes that had not previously been linked to this trait,providing insights complementary to those from *** summary,this study supports the application of TWAS for candidate gene identification in species with low rates of LD decay.

关键词： eQTLs TWAS structural variation alternative splicing soybean

Expression of NAC1.up-stream regulatory region and its relationship to the lateral root initiation induced by gibberellins and auxins

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science{1.(Life sciences) 2006年第5期49卷 429-435页

作者： wang Youhua 1.,DUAN Liusheng 1.,LU Mengzhu 2 ,LI Zhaohu 1.,wang Minjie 2 &ZHAI Zhixi 1.1.State Key Laboratory of Plant Physiology and Biochemistry,Department of Agronomy,college of Agronomy and Biotech- nology,china agricultural university,beijing 1.0094,china 2.Forestry Institute,Chinese Academy of Forestry,beijing 1.0091.china State Key Laboratory of Plant Physiology and Biochemistry Department of Agronomy College of Agronomy and Biotechnology China Agricultural University Beijing 100094 China Forestry Institute Chinese Academy of Forestry Beijing 100091 China

A 1.50 bp up-stream regulatory fragment of the transcription factor gene NAC1.n Arabidopsis thaliana was isolated using polymerase chain reaction (PCR) based techniques. Thefragment was used to substitute the 35S promoter of the pBI1.1.plasmid to construct abate-glucuronidase gene (GUS) expression system. The construct was introduced into tobacco(Nico-tiana tabaccum) plants by the Agrobacterium-med\aled transferring method. GUS expressionpattern was studied by using the transgenic lines. The results showed that the GUS driven by theNAC1.up-stream regulatory region was specifically expressed in the root meristem region, basal areasof the lateral root primordium and the lateral roots. The GUS expression was induced by3-indolebutyric acid (IBA)and gibberellins (GA_3 and GA_(4+7)). The results indicated that theup-stream regulatory fragment of NAC1.responded to plant hormones. The fragment might be involved inboth auxins and gibberellins signaling in promoting the development of lateral roots.

关键词： auxin gibberellin GUS NAC1 lateral root Nicotians tabaccum

Spatial distribution regularity and influence factors of population density in the LRGR

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science Bulletin 2007年第S2期 90-97页

作者： SONG GuoBao~(1,2) LI ZhengHai~(1,3+) BAO YaJing~1 L HaiYan~4 gao JiXi~3 wang HaiMei~5 XU Tian~6 CHENG Yan~7 1 Institute of Bio-Resources and Environment,college of Life science,Dalian Nationalities university,Dalian 116600,china 2 college of Resources science & Technology,beijing Normal university,beijing 100875,china 3 Innovation Base of Regional Ecology,Chinese Research Academy of Environmental sciences,beijing 100012,china 4 Institute of agricultural Resources and Regional Planning,Chinese Academy of agricultural sciences,beijing 100081,china 5 Inner Mongolia Municipality Observatory,Huhhot 010051,china 6 college of Life science,Inner Mongolia university,Huhhot 010021,china 7 college of Ecological Environment,Inner Mongolia agricultural university,Huhhot 010018,china

Combining GIS measures and statistic methods,this paper selects the physiographic factors and climatic factors to study the influencing strength and its functional forms of all factors on population density in the *** addition,this paper simulates the status of population density distribution in the LRGR with the factors influencing the population density *** findings indicate the following circumstances:(1.All natural factors have influence on the spatial pattern of population density in the LRGR,but the influencing extent and forms are quite *** factors and traffic network density have great influence on population density,while such factors as temperature and precipitation have little influence on population *** relationship between the popula- tion density and the altitude and traffic network density assumes the negative and positive linear cor- relation trends,while the influence of water system density and slope appears to be the exponential function and logarithmic function separately.(2)The population is scattered throughout LRGR and takes on such features as integral scattering and local concentration because of the influence of natural ***,a high population density belt has been formed in the middle of LRGR,and a high population density patch comes into being in the south of LRGR.(3)There is a good linear re- gression relationship between population density and human activity index(HAI)in the LRGR,which reflects that population density can present the artificial disturbance on natural ecosystem in the LRGR. (4)The increase of population density leads to the decrease of NDVI index.

关键词： population density artificial disturbance vegetation coverage physiographic factor LRGR

Molecular cloning and expression of interleukin Ibeta (IL-1.) from red seabream (Pagrus major)

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Progress in Natural science:Materials International 2004年第5期 22-28页

作者： CAI Zhonghua, SONG Linsheng, gao Chunping, WU Longtao and QIU Lihua (1. Institute of Oceanology, Chinese Academy of {1.s, Qingdao 266071. china 2. Department of Fisheries {1., Tianjin agricultural college, Tianjin 300381. china 3. The college of Life {1., Ocean university of china, Qingdao 266003, china)

The interleukin 1. (IL-1.) cDNA was cloned from the red seabream (Pagrus major) by homology cloning strategy. A cDNA fragment was amplified by PCR using two degenerated primers, which were designed according to the conserved regions of other known IL-1. sequences, and elongated by 3' ends and 5' ends RACE PCR to get the full length coding sequence of red seabream IL-1. (RS IL-1.). The sequence contained 1.52 nucleotides that included a 5' untranslated region (UTR) of 84bp, a 3' UTR of 41. bp and an open reading frame (ORF) of 759 nucleotides which could be translated into a putative peptide of 253 amino acids with molecular weight of 28.6 kD and putative isoelectric point pI of 5.29. The deduced peptide contained two potential N-glycosylation sites and an identifiable IL1.family signature, but lacked the signal peptide and the clear ICE cut site, which were common in other nonmammalian IL-1. genes. The RS IL-1. had the highest homology with piscine IL-1. according to phylogenetic tree analysis.

关键词： Interleukin-1. homology cloning mRNA expression red seabream.

Structure,Binding Characteristics,and 3D Model Prediction of a Newly Identified Odorant-Binding Protein from the Cotton Bollworm,Helicoverpa armigera (Hübner)

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Journal of Integrative Agriculture 2012年第3期11卷 430-438页

作者： ZHANG Tian-tao wang Wei-xuan GU Shao-hua ZHANG Zi-ding WU Kong-ming ZHANG Yong-jun GUO Yu-yuan Northwest Agriculture and Forestry university Yangling 712100 P.R. China State Key Laboratory of Plant Diseases and lnsect Pests/Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing 100193 P.R. China State Key Laboratory of Agrobiotechnology/college of Biological {1.s China Agricultural University Beijing 100193 P.R. China

The full-length sequence of the odorant binding protein 5 gene,HarmOBP5,was obtained from an antennae cDNA library of cotton bollworm,Helicoverpa armigera （Hübner）.The cDNA contains a 444 bp open reading frame,encoding a protein with 1.7 amino acids,namely ***5 was expressed in Escherichia coli and the recombinant protein was purified by affinity ***-PAGE and Western blot analysis demonstrated that the purified protein can be used for further investigation of its binding *** binding assays with 1.3 odorant chemicals indicated that HarmOBP5 has strong affinity to some special plant volatiles,including （E）-β-farnesene,ethyl butyrate,ethyl heptanoate,and acetic acid 2-methylbutyl *** on three-dimensional （3D） model of AaegOBP1.from Aedes aegypti,a 3D model of HarmOBP5 was *** model revealed that some key binding residues in HarmOBP5 may play important roles in odorant perception of *** study provides clues for better understanding physiological functions of OBPs in *** and other insects.

关键词： odorant binding protein fluorescence binding assay 3D model Helicoverpa armigera (Hübner)

Study on the spatial differences and its time lag effect on climatic factors of the vegetation in the Longitudinal Range-Gorge Region

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science Bulletin 2007年第S2期 42-49页

作者： BAO YaJing~1 SONG GuoBao~(1,2) LI ZhengHai~(1,3) gao JiXi~3 L HaiYan~4 wang HaiMei~5 CHENG Yan~6 XU Tian~7 1 Institute of Bio-Resources and Environment,college of Life science,Dalian Nationalities university,Dalian 116600,china 2 college of Resources science and Technology,beijing Normal university,beijing 100875,china 3 Innovation Base of Regional Ecology,Chinese Research Academy of Environmental sciences,beijing 100012,china 4 Institute of agricultural Resources and Regional Planning,Chinese Academy of agricultural sciences,beijing 100081,china 5 Inner Mongolia Municipality Observatory,Huhhot 010051,china 6 college of Ecological Environment,Inner Mongolia agricultural university,Huhhot 010018,china 7 college of Life science,Inner Mongolia university,Huhhot 010021,china

This article analyzes the correlation of NDVI and two meteorological data including temperature and precipitation,and probes the spetial pattern of responding sensitivity of the vegetation to temperature and precipitation *** constructing the NDVIrindex,the relation between vegetation cover and land use in the Longitudinal Range-Gorge Region（LRGR）is *** conclusion is as follows:（1. The time lag effect that vegetation responds to temperature and precipitation change is ***- ferent regions have different delay time corresponding to maximum correlation *** to response time of precipitation and temperature combination,three kinds of temperature-precipi- tation forms are defined,namely,precipitation and temperature in the same time,precipitation prior to temperature and temperature prior to precipitation.（2）The spatial distribution of sensitivity,of which vegetation responses to temperature,is characterized by several high and low scattered correlation *** from temperature,two insensitive regions and two sensitive belts are formed to pre- sent the response of vegetation to precipitation.（3）The NDVIris constructed,which can perfectly re- flect the corresponding relationship between meteorological factors and land use condition in the LRGR.

关键词： land cover meteorological factors effect of time lag spatial correlation population density Longitudinal Range-Gorge Region(LRGR)

Enhanced potency of an IgM-like nanobody targeting conserved epitope in SARS-CoV-2 spike N-terminal domain

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Signal Transduction and Targeted Therapy 2024年第6期9卷 2726-2737页

作者： Bo Liu Honghui Liu Pu Han Xiaoyun wang Chunmei wang Xinxin Yan Wenwen Lei Ke Xu Jianjie Zhou Jianxun Qi Ruiwen Fan Guizhen Wu Wen-xia Tian George F.gao Qihui wang college of Veterinary Medicine Shanxi Agricultural University030801 JinzhongChina CAS Key Laboratory of Pathogen Microbiology and Immunology Institute of MicrobiologyChinese Academy of Sciences(CAS)100101 BeijingChina School of Life sciences Yunnan University650504 KunmingYunnan ProvinceChina NHC Key Laboratory of Biosafety National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and Prevention(China CDC)102206 BeijingChina Savaid Medical School University of Chinese Academy of Sciences101408 BeijingChina

Almost all the neutralizing antibodies targeting the receptor-binding domain(RBD)of spike(S)protein show weakened or lost efficacy against severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)emerged or emerging variants,such as Omicron and its *** suggests that highly conserved epitopes are crucial for the development of neutralizing ***,we present one nanobody,N235,displaying broad neutralization against the SARS-CoV-2 prototype and multiple variants,including the newly emerged Omicron and its ***-electron microscopy demonstrates N235 binds a novel,conserved,cryptic epitope in the N-terminal domain(NTD)of the S protein,which interferes with the RBD in the neighboring S *** neutralization mechanism interpreted via flow cytometry and Western blot shows that N235 appears to induce the S1.subunit shedding from the trimeric S ***,a nano-IgM construct(MN235),engineered by fusing N235 with the human IgM Fc region,displays prevention via inducing S1.shedding and cross-linking virus *** to N235,MN235 exhibits varied enhancement in neutralization against pseudotyped and authentic viruses in *** intranasal administration of MN235 in low doses can effectively prevent the infection of Omicron sub-variant BA.1.and XBB in vivo,suggesting that it can be developed as a promising prophylactic antibody to cope with the ongoing and future infection.

关键词： doses prevention conserved

Chemical construction and anti-HCoV-OC43 evaluation of novel 1.,1.-disubstituted aloperine derivatives as dual cofactor inhibitors of TMPRSS2 and SR-B1

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Chinese Chemical Letters 2024年第5期35卷 201-206页

作者： Yulong Shi Fenbei Chen Mengyuan Wu Xin Zhang Runze Meng Kun wang Yan wang Yuheng Mei Qionglu Duan Yinghong Li Rongmei gao Yuhuan Li Hongbin Deng Jiandong Jiang Yanxiang wang Danqing Song beijing Key Laboratory of Antimicrobial Agents Institute of Medicinal BiotechnologyChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100050China Department of Pharmacy Affiliated Hospital of Jining Medical UniversityJining Medical UniversityJining 272000China

Thirty-one new 1.,1.-disubstituted aloperine derivatives were subtly constructed through a selective oxidation on the 1.-α-C-H induced by sulfonyl and a nucleophilic substitution with the stereoselectivity and *** them,compound 6b displayed a moderate anti-human coronavirus OC43(HCoV-OC43)potency and blocked the viral entry stage through a host mechanism of *** chemoproteomic techniques,both transmembrane serine protease 2(TMPRSS2)and scavenger receptor class B type 1.SR-B1.proteins,which act as host cofactors of viral entry,were identified to be the direct targets of 6b against ***,6b may deactivate the TMPRSS2 by inducing a change in protein conformation,rather than binding to its catalytic center,thus suppressing the viral membrane ***,our study provided key scientific data for the development of aloperine derivatives into a new class of antiviral candidates against humanβ-coronavirus,including severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).

关键词： Aloperine SARS-CoV-2 TMPRSS2 SR-B1 Synthesis