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Calcium-based polymers for suppression of soil acidification by improving acid-buffering capacity and inhibiting nitrification

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Journal of Environmental sciences 2024年第5期139卷 138-149页

作者： Fei Kang Yunshan Meng Yanning Ge Yun Zhang Haixiang gao Xueqin Ren Jie wang Shuwen Hu beijing Key Laboratory of Farmland Soil Pollution Prevention-control and Remediation College of Resources and Environmental SciencesChina Agricultural UniversityBeijing 100193China Department of Applied Chemistry China Agricultural UniversityBeijing 100193China

Soil acidification is a major threat to agricultural sustainability in tropical and subtropical *** and environmentally friendly materials,such as calcium lignosulfonate(CaLS),calcium poly(aspartic acid)(PASP-Ca),and calcium polyγ-glutamic acid(γ-PGA-Ca),are known to effectively ameliorate soil ***,their effectiveness in inhibiting soil acidification has not been *** study aimed to evaluate the effect of CaLS,PASP-Ca,andγ-PGA-Ca on the resistance of soil toward acidification as directly and indirectly(i.e.,via nitrification)caused by the application of HNO_(3)and urea,*** comparison,Ca(OH)_(2)and lignin were used as the inorganic and organic controls,*** the materials,γ-PGA-Ca drove the substantial improvements in the pH buffering capacity(pHBC)of the soil and exhibited the greatest potential in inhibiting HNO_(3)-induced soil acidification via protonation of carboxyl,complexing with Al~(3+),and cation exchange *** acidification induced by urea,CaLS was the optimal one in inhibiting acidification and increasing exchangeable acidity during ***,the sharp reduction in the population sizes of ammonia-oxidizing bacteria(AOB)and ammonia-oxidizing archaea(AOA)confirmed the inhibition of nitrification via CaLS ***,compared to improving soil pHBC,CaLS may play a more important role in suppressing indirect ***,γ-PGA-Ca was superior to PASP-Ca and CaLS in enhancing the soil pHBC and the its resistance to acidification induced by HNO_(3) addition,whereas CaLS was the best at suppressing urea-driven soil acidification by inhibiting *** conclusion,these results provide a reference for inhibiting soil re-acidification in intensive agricultural systems.

关键词： Calcium-based polymer Soil acidification pH buffering capacity Nitrification inhibition

Oils with different degree of saturation:effects on ileal digestibility of fat and corresponding additivity and bacterial community in growing pigs

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Journal of Animal science and Biotechnology 2024年第4期15卷 1657-1668页

作者： Lu wang Yifan Chen Yuansen yang Nuo Xiao Changhua Lai State Key Laboratory of Animal Nutrition Ministry of Agriculture and Rural Affairs Feed Industry CentreChina Agricultural UniversityNo.2 Yuanmingyuan West RoadHaidian DistrictBeijing 100193China college of Animal science and Technology Hebei Agricultural UniversityHebei 071000China

Background Oils are important sources of energy in pig *** combination of oils with different degree of saturation contributes to improve the utilization efficiency of the mixed oils and may reduce the cost of oil *** experiment was conducted to evaluate the effects of oils with different degree of saturation on the fat digestibility and corresponding additivity and bacterial community in growing *** Eighteen crossbred(Duroc×Landrace×Yorkshire)barrows(initial body weight:29.3±2.8 kg)were surgically fitted with a T-cannula in the distal *** experimental diets included a fat-free basal diet and 5 oil-added *** 5 oil-added diets were formulated by adding 6%oil with different ratio of unsaturated to saturated fatty acids(U:S)to the basal *** 5 oils were palm oil(U:S=1.2),canola oil(U:S=12.0),and palm oil and canola oil were mixed in different proportions to prepare a combination of U:S of 2.5,3.5 and 4.5,*** The apparent and standardized ileal digestibility(AID and SID)of fat and fatty acids increased linearly(P18:*** AID and SID of fat and fatty acids differed among the dietary treatments(P18:*** one-slope broken-line analyses for the SID of fat,saturated fatty acids(SFA)and UFA indicated that the breakpoint for U:S of oil was 4.14(R^(2)=0.89,P1),2.91(R^(2)=0.98,P1)and 3.84(R^(2)=0.85,P1),*** determined SID of fat,C18:1,C18:2 and UFA in the mixtures was not different from the calculated SID of fat,C18:1,C18:2 and ***,the determined SID of C16:0,C18:0 and SFA in the mixtures were greater than the calculated SID values(P16:0,C18:0 and SFA(P<0.05).

关键词： Additivity Bacterial community Fat and fatty acids Growing pigs Ratio of unsaturated to saturated fatty acids Standardized ileal digestibility

Identification of S-RNase genotype and analysis of its origin and evolutionary patterns in Malus plants

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Journal of Integrative Agriculture 2024年第4期23卷 1205-1221页

作者： Zhao Liu Yuan gao Kun wang Jianrong Feng Simiao Sun Xiang Lu Lin wang Wen Tian Guangyi wang Zichen Li Qingshan Li Lianwen Li Dajiang wang Research Institute of Pomology Chinese Academy of Agricultural Sciences/Key Laboratory of Horticultural Crops Germplasm Resources UtilizationMinistry of Agriculture and Rural AffairsXingcheng 125100China college of Agriculture Shihezi University/Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization of Xinjiang Production and Construction CorpsShihezi 832000China

Identification of the S genotype of Malus plants will greatly promote the discovery of new genes,the cultivation and production of apple,the breeding of new varieties,and the origin and evolution of self-incompatibility in Malus *** this experiment,88 Malus germplasm resources,such as Aihuahong,Xishuhaitang,and Reguanzi,were used as *** gene-specific primer combinations were used in the genotype *** amplification using leaf DNA produced a single S-RNase gene fragment in all *** results revealed that 70 of the identified materials obtained a complete S-RNase genotype,while only one S-RNase gene was found in 18 of *** homology comparison and analysis,13 S-RNase genotypes were obtained:S_(1)S_(2)(Aihuahong,etc.),S_(1)S_(28)(Xixian Haitang,etc.),S_(1)S_(51)(Hebei Pingdinghaitang),S_(1)S_(3)(Xiangyangcun Daguo,etc.),S_(2)S_(3)(Zhaiyehaitang,etc.),S_(3)S_(51)(Xishan 1),S_(3)S_(28)(Huangselihaerde,etc.),S_(2)S_(28)(Honghaitang,etc.),S_(4)S_(28)(Bo 11),S_(7)S_(28)(Jiuquan Shaguo),S_(10)S_e(Dongchengguan 13),S_(10)S_(21)(Dongxiangjiao)and S_(3)S_(51)(Xiongyue Haitang).Simultaneously,the frequency of the S gene in the tested materials was *** findings revealed that different S genes had varying frequencies in Malus resources,as well as varying frequencies between intraspecific and interspecific.S_(3) had the highest frequency of 68.18%,followed by S_(1)(42.04%).In addition,the phylogenetic tree and origin evolution analysis revealed that the S gene differentiation was completed prior to the formation of various apple species,that cultivated species also evolved new S genes,and that the S_(50) gene is the oldest S allele in Malus *** S_(1),S_(29),and S_(33) genes in apple-cultivated species,on the other hand,may have originated in ***,***,and ***,*** addition to ***,*** and *** may have also played a role in the origin and evolution of some Chine

关键词： Malus S-RNase genotype self-incompatibility origin and evolution

Formation mechanism of fault accommodation zones under combined stress in graben basin:Implications from geomechanical modeling

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Petroleum science 2024年第1期21卷 54-76页

作者： Qi-Qiang Ren Jin-Liang gao Rong-Tao Jiang Jin wang Meng-Ping Li Jian-Wei Feng Institute of Sedimentary Geology Chengdu University of TechnologyChengdu 610059SichuanChina Petrochina Research Institute of Petroleum Exploration and Development Beijing100083China Jianghan Oilfield Oil and Gas Production Capacity Construction Management Center Chongqing408000China Sinopec Chongqing Fuling Shale Gas Exploration and Development Company Chongqing408000China college of Energy Chengdu University of TechnologyChengdu 610059SichuanChina School of Geoscience China University of Petroleum(East China)Qingdao 266580ShandongChina

A fault accommodation zone is a type of structure that is defined as regulating displacement and strain between faults *** numbers of fault accommodation zones are being identified in graben basins,indicating the potential exploration target and petroleum accumulation *** study aims to analyze the formation mechanism and development of fault accommodation zones under combined stress by a numerical simulation method considering geomechanical *** three-dimensional(3-D)seismic interpretation and fractal dimension method,exampled with the Dongxin fault zone,the fault activity and fault combination pattern were conducted to quantitatively characterize the activity difference in fault accommodation *** with mechanical experiment test,a geomehcanical model was established for fault accommodation zones in a graben *** the paleostress numerical simulations and structural physical simulation experiment,the developmental characteristics and genetic mechanism of fault accommodation zones were *** by multi movements and combined stresses,three significant tectonic evolution stages of the Dongxing Fault Zone(DXFZ)were distinguished:During the E_(s)^(3)sedimentary period,the large difference in the stress,strain,and rupture distribution in various faults were significant,and this stage was the key generation period for the prototype of the DXFZ,including the FAZ between large-scale *** the E_(s)^(2)sedimentary period,the EW-trending symmetric with opposite dipping normal faults and the NE-SW trending faults with large scale were furtherly *** junction area of two secondary normal faults were prone to be ruptured,performing significant period for inheriting and developing characteristics of fault accommodation *** the Es1 sedimentary period,the high-order faults in the DXFZ exhibited the obvious fault depressions and strike-slip activity,and the fault accommodation zones were

关键词： Fault accommodation zone Graben basin Fault activity Tectonic physical simulation experiment Finite element numerical simulation Dongxin fault zone

Natural variation of GmFNSII-2 contributes to drought resistance by modulating enzyme activity in soybean

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The Crop Journal 2024年第2期12卷 529-539页

作者： Huihui gao Pengcheng Wei Yongzhe Gu Pengbin Tang Yifan Shen Lei yang Linxin Dong Haowei Zheng Kuo Shu Mayamiko Masangano Bin Dong Long Miao Jiajia Li Lijuan Qiu Xiaobo wang School of Agronomy Anhui Agricultural UniversityHefei 230036AnhuiChina State Key Laboratory of Crop Gene Resources and Breeding The National Key Facility for Crop Gene Resources and Genetic Improvement(NFCRI)/Key Laboratory of Crop Gene Resource and Germplasm Enhancement(MOA)Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China

As an essential crop that provides vegetable oil and protein,soybean(Glycine max(L.)Merr.)is widely planted all over the ***,the scarcity of water resources worldwide has seriously impacted on the quality and yield of *** address this,exploring excellent genes for improving drought resistance in soybean is *** this study,we identified natural variations of GmFNSII-2(flavone synthase II)significantly affect the drought resistance of *** sequence analysis of GmFNSII-2 in 632 cultivated and 44 wild soybeans nine haplotypes were *** full-length allele GmFNSII-2^(C),but not the truncated allele GmFNSII-2^(A) possessing a nonsense nucleotide variation,increased enzyme *** research found that GmDREB3,known to increase soybean drought resistance,bound to the promoter region of GmFNSII-2^(C).GmDREB3 positively regulated the expression of GmFNSII-2^(C),increased flavone synthase abundance and improved the drought ***,a singlebase mutation in the GmFNSII-2^(C) promoter generated an additional drought response element(CCCCT),which had stronger interaction strength with GmDREB3 and increased its transcriptional activity under drought *** frequency of drought-resistant soybean varieties with Hap 1(Pro:GmFNSII-2^(C))has increased,suggesting that this haplotype may be selected during soybean *** summary,GmFNSII-2^(C) could be used for molecular breeding of drought-tolerant soybean.

关键词： Drought stress Flavone synthase GmFNSII-2 Natural variation Drought response element

Differential gene expression of phosphoglyceric kinase (PGK) and hypoxic adaptation in chicken

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science china(Life sciences) 2007年第3期50卷 335-342页

作者： wang CunFang1,3, YUAN CunZhong2, ZHANG Lao1, WU ChangXin1 & LI Ning2 1 college of Animal science and Technology, china agricultural university, beijing 100094, china 2 china State Key Laboratories for Agrobiotechnology, china agricultural university, beijing 100094, china 3 college of Food and Biologic Engineering, Shandong Institute of Light Industry, Jinan 250353, china college of Animal science and Technology China Agricultural University Beijing 100094 China college of Food and Biologic Engineering Shandong Institute of Light Industry Jinan 250353 China china State Key Laboratories for Agrobiotechnology China Agricultural University Beijing 100094 China college of Animal science and Technology China Agricultural University Beijing 100094 China

Four single-nucleotide polymorphisms (SNP) of the Phosphoglyceric Kinase (PGK) gene were discov- ered based on comparison of the sequences from an altiplano chicken breed (Tibetan chicken) and two lowland breeds (White Leghorn and Shouguang chicken). Gel-shift results indicate that one of these SNPs, an A→G mutation at position 59 in exon10, is able to bind hypoxia-induced factor-l (HIF-1), functioning as a hypoxia response element (HRE). The mutant gene results in M→T mutation at position 379 amino acid. The combined activity of this HRE and HIF-1 could increase correspondingly under a hypoxic stimulus. Hypoxia leads to increased death rates of chicken embryos; while the M→T mutation described herein is prevalent in healthy embryos grown under hypoxic conditions, thus it may repre- sent an adaptation to hypoxia. Fluorescence quantitative reverse transcription PCR results revealed that HIF-1 upregulates the transcript level of the glycolytic enzyme PGK in the brain and skeletal mus- cle of animals subjected to hypoxia. Thus, a large amount of ATP is produced by increased glycolysis, allowing the organism to meet energy metabolism demands. As such, we believe this SNP to be an adaptation to the external anoxic environment.

关键词： gene and hypoxic adaptation in chicken Differential gene expression of phosphoglyceric kinase PGK SNPs HRE

The single nucleotide polymorphisms of chicken melanocortin-4 receptor(MC4R)gene and their association analysis with carcass traits

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science china(Life sciences) 2006年第6期49卷 560-566页

作者： QIU Xuemei1,2,LI Ning1,DENG Xuemei3,ZHAO Xingbo3,MENG Qingyong1 & wang Xiuli2 1. State Key Laboratory for Agrobiotechnology,china agricultural university,beijing 100094,china 2. college of Life science and Technology,Dalian Fisheries university,Dalian,116023,china 3. college of Animal science and Technology,china agricultural university,beijing 100094,china State Key Laboratory for Agrobiotechnology China Agricultural University Beijing 100094 China college of Life science and Technology Dalian Fisheries University Dalian 116023 China State Key Laboratory for Agrobiotechnology China Agricultural University Beijing 100094 China college of Animal science and Technology China Agricultural University Beijing 100094 China college of Life science and Technology Dalian Fisheries University Dalian 116023 China

Mutations of the melanocortin-4 receptor (MC4R) gene are associated with the appetite, obesity; growth in pig, mice; human. But little is known about the function of chicken MC4R gene. In this study, F2 chicken resource population derived from broilers crossing to Silky was screened for the polymorphisms of the MC4R gene using PCR-single strand conformation polymorphism (PCR-SSCP); DNA sequencing methods. Four single nucleotide polymorphisms (SNPs) sites were found. The mutation (C→T) in the 5′ regulation region of chicken MC4R gene results in one more NF-E2; cap transcription factor binding sites in the mutation allele than in the wild allele. One missense mutation (G→A) occurs in the coding region (61nt), which changes the glycine to arginine. Moreover, in the coding region there are 2 synonymous mutations, one G→T mutation at 315nt; one C→T mutation at 336nt. Least square analysis of the SNPs; carcass traits showed that BB, DD; FF genotypes are significantly associated with body weight, carcass weight (or half carcass weight),; leg muscle weight (P>0.05 or P>0.01). But no significant association between the genotypes; abdominal fat weight is found. The results present the evidence that the chicken MC4R gene can be selected as the major candidate gene for the carcass traits such as body weight; growth.

关键词： melanocortin-4 receptor, SNPs, chicken, carcass traits.

Long noncoding RNAs HAND2-AS1 ultrasound microbubbles suppress hepatocellular carcinoma progression by regulating the miR-873-5p/tissue inhibitor of matrix metalloproteinase-2 axis

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World Journal of Gastrointestinal Oncology 2024年第4期16卷 1547-1563页

作者： Qiang Zou Hao-Wen wang Xi-Liang Di Yuan Li Hui gao Department of Interventional Therapy Tianjin Medical University Cancer Institute and HospitalTianjin 300060China National Clinical Research Center for Cancer Key Laboratory of Cancer Prevention and TherapyTianjin’s Clinical Research Center for CancerTianjin 300060China college of Animal science and Technology Northeast Agricultural UniversityHarbin 150030Heilongjiang ProvinceChina Department of Hematology and Oncology Linyi People’s HospitalLinyi 251500Shandong ProvinceChina Department of Comprehensive Oncology Baotou Cancer HospitalBaotou 014030Inner Mongolia Autonomous RegionChina

BACKGROUND Increasing data indicated that long noncoding RNAs(lncRNAs)were directly or indirectly involved in the occurrence and development of tumors,including hepatocellular carcinoma(HCC).Recent studies had found that the expression of lncRNA HAND2-AS1 was downregulated in HCC tissues,but its role in HCC progression is *** targeted microbubble destruction mediated gene transfection is a new method to overexpress *** To study the role of ultrasound microbubbles(UTMBs)mediated HAND2-AS1 in the progression of HCC,in order to provide a new reference for the treatment of *** In vitro,we transfected HAND2-AS1 siRNA into HepG2 cells by UTMBs,and detected cell proliferation,apoptosis,invasion and epithelial-mesenchymal transition(EMT)by cell counting kit-8 assay,flow cytometry,Transwell invasion assay and Western blotting,*** addition,we transfected miR-837-5p mimic into UTMBs treated cells and observed the changes of cell ***,the UTMBs treated HepG2 cells were transfected together with miR-837-5p mimic and tissue inhibitor of matrix metalloproteinase-2(TIMP2)overexpression vector,and we detected cell proliferation,apoptosis,invasion and *** vivo,we established a mouse model of subcutaneous transplantation of HepG2 cells and observed the effect of HAND2-AS1 silencing on tumor formation *** We found that UTMBs carrying HAND2-AS1 restricted cell proliferation,invasion,and EMT,encouraged apoptosis,and HAND2-AS1 silencing eliminated the effect of ***,miR-873-5p targets the gene HAND2-AS1,which also targets the 3’UTR of *** miR-873-5p mimic counteracted the impact of ***,miR-873-5p mimic solely or in combination with pcDNA-TIMP2 had been transformed into HepG2 cells exposed to *** discovered that TIMP2 reversed the effect of miR-873-5p mimic caused by the blocked signalling cascade for matrix metalloproteinase(MMP)2/*** vivo results showed that HAND2-AS1 silencing sig

关键词： Hepatocellular carcinoma Ultrasound microbubbles Long noncoding RNA HAND2-AS1 miR-873-5p Tissue inhibitor of matrix metalloproteinase-2

Variations in carbon isotope ratios of C_3 plants and distribution of C_4 plants along an altitudinal transect on the eastern slope of Mount Gongga

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science china Earth sciences 2009年第10期52卷 1714-1723页

作者： LI JiaZhu1, wang GuoAn2, LIU XianZhao1, HAN JiaMao3, LIU Min4 & LIU XiaoJuan2 1 college of Geography and Planning, Ludong university, Yantai 264025, china 2 Key Laboratory of Plant-Soil Interactions, Ministry of Education, college of Resources and Environmental sciences, china Agri- cultural university, beijing 100193, china 3 Institute of Geology and Geophysics, Chinese Academy of sciences, beijing 100029, china 4 college of Environmental sciences, MOE Laboratory for Earth Surface Processes, Peking university, beijing 100871, china college of Geography and Planning Ludong University Yantai China Key Laboratory of Plant-Soil Interactions Ministry of Education College of Resources and Environmental Sciences China Agricultural University Beijing China Institute of Geology and Geophysics Chinese Academy of Sciences Beijing China college of Environmental sciences MOE Laboratory for Earth Surface Processes Peking University Beijing China

Variations in carbon isotopic ratios (δ 13C) of C3 plants and distribution of C4 plants were investigated along an altitudinal transect on the eastern slope of Mount Gongga, and the environmental effects on them were discussed. It is shown that plants with C4 photosynthetic pathway mainly occur at altitudes below 2100 m a.s.l., suggesting that the low summer temperature is responsible for the distributional pattern. In addition, δ 13C of C3 plants increases with elevation at the region above 2000 m a.s.l. with the characteristics of humid climate, and the increase rate in δ 13C for C3 plants is about 1.3‰ per kilometer. Temperature determines the altitudinal trend of δ 13C.

关键词： Mount Gongga carbon isotopic ratios C3/C4 plants C4 distribution altitude