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Journal of Integrative Agriculture 2021年第3期20卷 804-814页

作者： HE Li-mei zhao Sheng-yuan gao Xi-wu WU Kong-ming State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China college of Plant Protection China Agricultural UniversityBeijing 100193P.R.China

Spodoptera frugiperda,the pest fall armyworm(FAW),is widespread in more than 100 *** date,planting insectresistant transgenic crops is one of the main control methods in its native *** this study we evaluated Bt-transgenic maize(Bt maize)and non-transgenic(conventional)maize and six other host plants in greenhouse pot experiments and field trials for oviposition preference by the Chinese populations of *** laboratory trials,female moths preferred to oviposit on maize with no significant preference between conventional and Bt ***,after conventional and transgenic maize were exposed to FAW larvae and damaged,oviposition was significantly higher on transgenic maize than on the conventional *** trials showed that for plants less damaged at an early stage(seedling stage),oviposition of FAW on transgenic and conventional maize was significantly higher than that on wheat,sorghum,foxtail millet,peanut and soybean while showing no significant difference between transgenic or conventional *** adults mainly laid eggs on Bt maize,while the larval density and leaf damage rating or percentage of damaged plants were significantly lower than on conventional *** density and its damage on conventional maize were significantly higher than that on Bt maize and the other five ***,maize is a highly preferred and suitable host for *** feeding and ovipositing,and Bt maize can be used as trap crop to protect other crops.

关键词： fall armyworm oviposition behavior trap crop Bt maize

7种十字花科绿肥作物对干旱胁迫的生理应答及抗旱性评价

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植物营养与肥料学报 2024年第9期30卷 1833-1844页

作者：孙艳妮王佳蓉韩高海唐云丽魏奭杰贾振鹏秦璐杨璐曹卫东黄冬琳高亚军王朝辉张达斌西北农林科技大学资源环境学院陕西杨凌712100 永寿县农业技术推广中心陕西永寿713400 中国农业科学院油料作物研究所/农业农村部油料作物生物学与遗传育种重点开放实验湖北武汉430062 中国农业科学院农业资源与农业区划研究所北京100081 农业农村部西北植物营养与农业环境重点实验室陕西杨凌712100 作物抗逆与高效生产全国重点实验室陕西杨凌712100

【目的】研究干旱胁迫对十字花科绿肥作物生育期生长及生理特性的影响,为筛选适宜的十字花科绿肥作物品种提供理论依据和关键技术参数,助力我国旱地农业绿色发展。【方法】以7种十字花科绿肥为研究对象,通过室内盆栽试验模拟干旱胁迫环... 详细信息

【目的】研究干旱胁迫对十字花科绿肥作物生育期生长及生理特性的影响,为筛选适宜的十字花科绿肥作物品种提供理论依据和关键技术参数,助力我国旱地农业绿色发展。【方法】以7种十字花科绿肥为研究对象,通过室内盆栽试验模拟干旱胁迫环境,分析正常灌水(田间持水量80%)和干旱胁迫(田间持水量40%)条件下7种十字花科绿肥作物生育期内生长指标、光合特性和生理特征的变化规律,并通过主成分分析法对其抗旱性进行综合评价,最终筛选出抗旱能力突出的十字花科绿肥作物品种。【结果】1)与正常灌水相比,干旱胁迫处理下各绿肥作物叶片的净光合速率和蒸腾速率均下降(P2)干旱胁迫条件下,绿肥作物发芽率、根长和生物量分别平均比正常灌水降低6.1%,147%(P238%(P3)干旱胁迫条件下,秦优10号叶片过氧化氢酶活性显著增加26%,秦优10号、青杂11号叶片超氧化物歧化酶活性显著增加,7种绿肥叶片过氧化物酶活性均呈升高趋势(P>0.05);4)干旱胁迫会引起中绿油2号、紫芥、秦优10号和中油肥1号叶片脯氨酸含量显著增加,紫芥、中绿油1号、中绿油2号丙二醛含量显著降低;5)主成分分析结果表明,生物量和叶片丙二醛含量、过氧化氢酶及过氧化物酶活性可作为评估十字花科绿肥抗旱性的首选抗旱指标,中绿油1号在供试7种十字花科绿肥的抗旱能力方面综合评分最高,抗旱能力较强。【结论】中绿油1号因其叶片较高的抗氧化能力、渗透调节能力及较长根系长度,在干旱胁迫条件下综合抗旱能力突出,可作为抗旱型十字花科绿肥品种在黄土高原地区推广应用。

关键词：干旱胁迫十字花科绿肥光合特性生理特征主成分分析

HIF-1α信号通路对脂多糖诱导鸡巨噬细胞炎症的调节作用研究

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中国畜牧兽医 2024年第5期51卷 2071-2080页

作者：冯晓梦高超陶新磊李小方吕晓萍高雪丽赵一李亚楠刘超男东北农业大学动物医学院黑龙江省实验动物与比较医学重点实验室哈尔滨150030 中国农业大学动物医学院北京100193

【目的】探究鸡巨噬细胞(HD11)在脂多糖(LPS)诱导条件下,缺氧诱导因子-1α(HIF-1α)信号通路和线粒体功能对细胞炎症反应的影响。【方法】试验以HD11细胞为研究对象,分别用不同浓度LPS作用于细胞,培养24 h后检测细胞活力,筛选LPS最佳作... 详细信息

【目的】探究鸡巨噬细胞(HD11)在脂多糖(LPS)诱导条件下,缺氧诱导因子-1α(HIF-1α)信号通路和线粒体功能对细胞炎症反应的影响。【方法】试验以HD11细胞为研究对象,分别用不同浓度LPS作用于细胞,培养24 h后检测细胞活力,筛选LPS最佳作用浓度;同时在最佳LPS作用浓度下,筛选LPS最佳作用时间。将HD11细胞分为对照组和模型组,模型组加入最佳作用浓度LPS培养,对照组加等量的完全培养液,按照LPS最佳作用时间培养后,提取细胞总RNA进行转录组测序,并对差异表达基因进行GO功能注释和KEGG通路富集分析。利用实时荧光定量PCR和Western blotting分别检测HIF-1α信号通路相关因子mRNA和蛋白表达量;通过流式细胞术检测线粒体膜电位和活性氧(ROS)水平变化。【结果】LPS诱导的HD11细胞炎症模型中最适条件为1μg/mL LPS培养12 h,以此条件成功建立了细胞炎症模型。与对照组相比,模型组共检测到2063个差异表达基因,其中1319个上调,744个下调。GO功能注释结果显示,差异表达基因显著富集到免疫系统应答、对外部刺激的反应和细胞因子受体结合等过程。KEGG通路富集分析结果显示,差异表达基因显著富集在50条信号通路,主要涉及免疫细胞介导的炎症反应和Toll样受体、核转录因子-κB(NF-κB)、HIF-1α等信号通路。其中有13个显著上调表达的基因集中在HIF-1α相关信号通路,包括Toll样受体4(TLR4)、NF-κB、HIF-1α、血管内皮生长因子(VEGF)基因等。实时荧光定量PCR和Western blotting结果显示,NF-κB p65、HIF-1α、VEGF等mRNA和蛋白表达水平均显著上调(P1),ROS水平显著升高(P1α与NF-κB信号通路相互串扰共同参与LPS诱导的细胞炎症过程。同时,细胞线粒体功能下降,导致ROS生成增多,进而促进HIF-1α的表达,共同加重了炎性反应和代谢紊乱。

关键词：鸡巨噬细胞脂多糖(LPS) HIF-1α 炎症反应线粒体功能

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G-protein β subunit AGB1 positively regulates salt stress tolerance in Arabidopsis

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Journal of Integrative Agriculture 2015年第2期14卷 314-325页

作者： MA Ya-nan CHEN Ming xu Dong-bei FANG Guang-ning WANG Er-hui gao Shi-qing xu zhao-shi LI Lian-cheng ZHANG Xiao-hong MIN Dong-hong MA You-zhi college of Agronomy Northwest A&F University/State Key Laboratory of Arid Region Crop Adversity Biology Institute of Crop sciences Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement/Key Laboratory of Biology and Genetic Improvement of Triticeae CropsMinistry of Agriculture beijing Hybrid Wheat Engineering and Technology Research Center

The heterotrimeric GTP-binding proteins（G-proteins） in eukaryotes consisted of α, β and γ subunits and are important in molecular signaling by interacting with G-protein-coupled receptors（GPCR）, on which to transduce signaling into the cytoplast through appropriate downstream effectors. However, downstream effectors regulated by the G-proteins in plants are currently not well defined. In this study, the transcripts of AGB1, a G protein β subunit gene in Arabidopsis were found to be down-regulated by cold and heat, but up-regulated by high salt stress treatment. AGB1 mutant（agb1-2） was more sensitive to high salt stress than wild-type（WT）. Compared with WT, the cotyledon greening rates, fresh weight, root length, seedling germination rates and survival rates decreased more rapidly in agb1-2 along with increasing concentrations of Na Cl in normal（MS） medium. Physiological characteristic analysis showed that compared to WT, the contents of chlorophyll, relative proline accumulation and peroxidase（POD） were reduced, whereas the malonaldehyde（MDA） content and concentration ratio of Na＋/K＋ were increased in agb1-2 under salt stress condition. Further studies on the expression of several stress inducible genes associated with above physiological processes were investigated, and the results revealed that the expressions of genes related to proline biosynthesis, oxidative stress response, Na＋ homeostasis, stress- and ABAresponses were lower in agb1-2 than in WT, suggesting that those genes are possible downstream genes of AGB1 and that their changed expression plays an important role in determining phenotypic and physiologic traits in agb1-2. Taken together, these findings indicate that AGB1 positively regulates salt tolerance in Arabidopsis through its modulation of genes transcription related to proline biosynthesis, oxidative stress, ion homeostasis, stress- and ABA-responses.

关键词： Arabidopsis heterotrimeric G-protein β subunit physiological processes salt stress tolerance

Enriched endoplasmic reticulum-mitochondria interactions result in mitochondrial dysfunction and apoptosis in oocytes from obese mice

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Journal of Animal science and Biotechnology 2018年第1期9卷 91-98页

作者： Lihong zhao Tengfei Lu Lei gao Xiangwei Fu Shien Zhu Yunpeng Hou State Key Laboratory of Agrobiotechnolocjy College of Biolocjical Sciences China Agricultural University Yuanmincjyuan west Rd 2 Bejing100193 China Key Laboratory of Animal Genetics Breeding and Reproduction College of Animal Science and Technology China Agricultural University Beijing China

Background： Maternal obesity alters oocytes and subsequent fetal metabolism. An increasing number of studies have shown that the endoplasmic reticulums（ER） or mitochondria have important effects on oocyte quality, but there has been no study of the effect of mitochondria-associated ER membranes（MAMs） on oocyte quality. The present study was designed to assess whether the level of MAM and MAM-related proteins were different in oocytes from obese and control ***： First, oocytes from mice with high-fat-diet（HFD）-induced obesity had higher levels（either greater numbers or a higher proportion for the same numbers） of MAM than oocytes from control mice. The abundance of MAM-related proteins in oocytes from obese mice was significantly greater at both the messenger RNA and protein levels, including inositol 1,4,5-trisphosphate receptor, type 1（IP3R1）, inositol 1,4,5-trisphosphate receptor,type 2（IP3 R2） and phosphofurin acidic cluster sorting protein 2（PACS-2）. Further, there was an increase in mitochondrial Ca^（2＋）（[Ca^（2＋）]_m） which was associated with increased apoptosis and compromised cytoplasmic maturation in oocytes from obese mice. Down-regulation of MAM-related protein IP3R1 in oocytes from obese mice decreased [Ca^2＋]_mand apoptosis and improved cytoplasmic maturation but did not reduce the overal MAM level. However, down-regulating MAM-related protein PACS-2 in oocytes from obese mice did reduce the level of MAM and [Ca^（2＋）]_m, which decreased the rate of apoptosis and improved cytoplasmic maturation of oocytes from obese ***： It is possible that enriched MAM could increase [Ca^（2＋）]_m, and this increase has been found to be associated with increased apoptosis and compromised cytoplasmic maturation in oocytes from obese mice. This finding suggests a novel therapeutic target for obesity-induced oocyte defects.

关键词： Apoptosis IP3R1 MAM Mitochondrial Ca2+ Obesity Oocyte PACS-2

Anaerobic digestion of heat treated sludge liquor by pilot scale mesophilic EGSB reactor

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International Journal of agricultural and Biological Engineering 2016年第3期9卷 146-153页

作者： zhao Jing Qiao Wei Wang Wei gao Fang Xiao Zheng Biomass Engineering Center College of EngineeringChina Agricultural UniversityBeijing 100083China State R&D Center for Efficient Production and Comprehensive Utilization of Biobased Gaseous Fuels Energy AuthorityNational Development and Reform Committee(BGFeuls)Beijing 100083China School of Environment Tsinghua UniversityBeijing 100084China Center for Environmental Education and Communications of Ministry of Environmental Protection Beijing 100029China beijing Zhongyuan Chuangneng Engineering&Technology CO. LTDBeijing 100080China

Biogas recovery from heat treated sludge liquor was tested by a 4 m^(3)mesophilic expanded granular sludge bed(EGSB)reactor within 206 *** pilot experiment,the heat pretreatment effects on hydrolysis and biogas production potential on three sludges from different wastewater plants were evaluated using bench *** of these sludges was used in the subsequent pilot heat treatment and EGSB *** biogas conversion potential of this sludge filtrate was 79%.Seeded with granular sludge,the EGSB reactor could be quickly activated and adapted to the new filtrate *** EGSB influent COD was 20.0-35.0 g/L,and the effluent COD was lower than 10.0 g/*** COD removal rate was 60%-70%at an organic loading rate(OLR)of 10.0 kg COD/(m^(3)·d).The fresh supernatant with low pH resulted in the high biogas production in *** effluent with high nitrogen and phosphor should be considered for further treatment.

关键词： anaerobic digestion sludge biogas heat treatment EGSB reactor pilot scale

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2023年中国蜂蜜贸易现状及未来展望

中国蜂业 2024年第6期75卷 53-57页

作者：安民辰谢崇鑫高芸赵芝俊中国农业科学院农业经济与发展研究所北京100081 青岛农业大学经济管理学院青岛266109

近年来,受进口增长以及出口减少的影响,中国蜂蜜贸易顺差不断缩小。本文利用贸易数据分析贸易结构和特征、研判贸易形势,并结合近年蜂蜜产品国内市场调查数据、进出口检验检疫公报以及蜂蜜品质和成分的最新研究,对我国蜂蜜出口态势和市... 详细信息

近年来,受进口增长以及出口减少的影响,中国蜂蜜贸易顺差不断缩小。本文利用贸易数据分析贸易结构和特征、研判贸易形势,并结合近年蜂蜜产品国内市场调查数据、进出口检验检疫公报以及蜂蜜品质和成分的最新研究,对我国蜂蜜出口态势和市场形势进行研判。研究认为,技术性贸易壁垒在一定程度上制约了我国蜂蜜出口量价增长,但更重要的是蜂蜜市场细分和产品品牌创建对进出口市场有较大影响。我国蜂产业亟待建立产品标准化体系,开发我国特色蜂蜜品种的质量标准和有益成分,同时开展消费者科普活动,借鉴国外蜂蜜品牌建设经验,宣传我国特色蜂蜜的保健功能,推广成熟蜜生产方式,建立蜂产品优质优价市场机制。

关键词：蜂蜜贸易进出口价格展望

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松墨天牛4龄越冬幼虫响应低温胁迫的生理适应机制

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北京林业大学学报 2024年第4期46卷 74-83页

作者：刘佳奇赵立娟刘磊黄瑞芬韩有志张志伟崔绍朋高瑞贺山西农业大学林学院山西太谷030800 山西省林业危险性有害生物检验鉴定中心山西太谷030800 国家林业和草原局生物灾害防控中心辽宁沈阳110034

【目的】明确松墨天牛4龄越冬幼虫响应低温胁迫的生理适应机制,为松墨天牛的科学防治提供理论依据。【方法】于2021年3月中旬于江西省德兴市采集松墨天牛4龄越冬幼虫,设置25℃(对照)、4、0、-5、-10、-15和-20℃7个温度梯度和30 min、1 ... 详细信息

【目的】明确松墨天牛4龄越冬幼虫响应低温胁迫的生理适应机制,为松墨天牛的科学防治提供理论依据。【方法】于2021年3月中旬于江西省德兴市采集松墨天牛4龄越冬幼虫,设置25℃(对照)、4、0、-5、-10、-15和-20℃7个温度梯度和30 min、1 h和3 h共3个时间梯度,分别测定虫体耐寒能力、耐寒物质含量、抗氧化酶活性以及低温胁迫对中肠损伤情况。【结果】(1)在过冷却点((-9.27±0.90)℃)附近,50%致死时间约为1.9 h(114.97 min),99%致死时间约为2.8 d(4061.86 min),在低于过冷却点(-15℃)的低温胁迫下,50%和99%致死时间约为-10℃低温处理下的1/4。(2)在低温胁迫处理后,4龄越冬幼虫虫体含水率变化不显著,脂肪含量和糖原质量浓度在0℃处理3 h后显著下降(P1 h后显著上升(P20℃处理1 h后显著下降(P3)低温胁迫处理后,抗氧化酶(SOD、POD、CAT)活性均呈显著上升趋势(P15℃处理3 h后中肠内壁分解,细胞之间出现空泡,-20℃处理3 h后中肠细胞破裂,细胞内容物逸散并充满整个肠腔,空泡消失。【结论】本文明确了松墨天牛4龄越冬幼虫响应低温胁迫的氧化应激生理反应,可丰富、完善松墨天牛耐寒机理研究。

关键词：松墨天牛越冬幼虫耐寒物质抗氧化酶系

Pyramiding of the dep1-1 and NAL1NJ6 alleles achieves sustainable improvements in nitrogen-use efficiency and grain yield in japonica rice breeding

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Journal of Genetics and Genomics 2019年第6期46卷 325-328页

作者： Xiaopeng xu Kun Wu Ruineng xu Jianping Yu Jing Wang Ying zhao Yun Wang Wenzhen Song Shuoxun Wang Zhi gao Yongjia Zhong Xinxin Li Hong Liao Xiangdong Fu State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental Biology Innovation Academy for Seed Design Chinese Academy of Sciences Beijing 100101 China Root Biology Center Fujian Agricultural and Forestry University Fuzhou 300052 China college of Life sciences University of Chinese Academy of Sciences Beijing 100049 China

Rice is one of the most important cereal crops in the world, and a substantial increase in grain yield is necessary for food security. However, high yields of semidwarf modern rice varieties are heavily dependent on the application of mineral nitrogenous fertilizer (Tilman et al., 2002;Sun et al., 2014). Nitrogen (N)-insensitive sponses associated with reduced N-use efficiency (NUE) is a major characteristic of the green revolution varieties (GRVs), in which the growth-inhibiting protein SLENDER RICE1 (SLR1) accumulates (Li et al., 2018). Unfortunately, increasing the level of N fertilizer use to reach the full yield potential of GRVs is subject to diminishing returns, quite apart from its deleterious effect on the environments (Rahn et al., 2009;Liu et al., 2015). Therefore, there is an urgent need to develop new rice GRVs that increase NUE while maintaining their high yields. Recently, several genes (e.g., DEP1, ***, OsNRT2.3b, ARE1 and GRF4) responsible for improved NUE have been identified in rice (Sun et al.. 2014;Hu et al., 2015;Fan et al., 2016;Wang et al., 2018;Li et al., 2018). More importantly, boosting the activity of the transcription factor GRF4 overcomes the ability of SLR1 to prevent the GRF4-GIF1 interaction, which in turn promotes the coordinated expression of the genes involved in N assimilation and carbon fixation and consequently enhances the NUE of rice GRVs, thereby improving our ability to grow crops sustainably (Li et al., 2018). However, current understanding of the genetic basis for improving NUE remains at the level of identification of a number of quantitative trait loci (QTLs), without any understanding of the nature of the gene products.

关键词： SLENDER RICE1 (SLR1) N-use efficiency (NUE) green revolution varieties (GRVs)

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新疆一枝蒿不同部位水提物的体外抗炎效果

中国兽医杂志 2024年第2期60卷 112-119页

作者：邢天骄彭凌峰马丹阳高雪嫣赵红琼郝智慧新疆农业大学动物医学学院新疆乌鲁木齐830052 中国农业大学动物医学院北京海淀100193

为了探究新疆一枝蒿不同部位水提物的体外抗炎效果,本试验在体外用脂多糖(LPS)诱导小鼠单核巨噬白血病细胞(RAW264.7)构建炎症模型,采用水提法对新疆一枝蒿不同部位(叶、茎、花)进行提取,运用噻唑蓝(MTT)法检测新疆一枝蒿不同部位水提... 详细信息

为了探究新疆一枝蒿不同部位水提物的体外抗炎效果,本试验在体外用脂多糖(LPS)诱导小鼠单核巨噬白血病细胞(RAW264.7)构建炎症模型,采用水提法对新疆一枝蒿不同部位(叶、茎、花)进行提取,运用噻唑蓝(MTT)法检测新疆一枝蒿不同部位水提物对细胞活力度的影响,实时荧光定量PCR(RT-qPCR)检测其对炎症相关基因TNF-α、IL-1β和IL-6 mRNA表达的影响,Western blot检测其对TNF-α、IL-1β和IL-6蛋白表达的影响。结果显示,新疆一枝蒿叶、茎、花水提物分别在0.078~1.250、0.078~5.000和0.078~1.250 mg/mL的浓度范围内对RAW264.7细胞活力度无显著抑制作用。与LPS模型组相比,新疆一枝蒿叶、茎、花水提物浓度范围在0.313~1.250、0.625~2.500和0.625~1.250 mg/mL时,细胞活力度分别提高了23.12%~28.14%(P1)、10.88%~18.62%(P1)和16.13%~16.76%(P1.250 mg/mL的叶水提物可以极显著降低TNF-α、IL-1β和IL-6的mRNA和蛋白表达(P1);浓度为0.625和1.250 mg/mL的茎水提物可以极显著降低IL-1β的mRNA和蛋白表达(P1),以及IL-6和TNF-α的mRNA表达(P1);浓度为0.625 mg/mL的花水提物可以极显著降低IL-1β和IL-6的mRNA和蛋白表达(P1)。结果表明,新疆一枝蒿不同部位(叶、茎、花)水提物均有抗炎效果,本试验结果可为新疆一枝蒿的兽医临床应用提供参考依据。

关键词：新疆一枝蒿不同部位脂多糖(LPS) RAW264.7 炎症