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cell Research 1991年第1期 35-43页

作者： gao qirong, konghua zhang, longsheng hong,chengtang xu AND guoyan gu (formerly KUOYENKU)shanghai institute of cell biology, academia sinica

The region between the preexisting and nascentmembranes of a cleaving Rana egg is a dense protrusionregion of nearly 40μm wide at 180°***,thisregion differentiate,into an upper part,a strip withlong, branched and randomly distributed protrusionswhich are derived largely from the preexistingmembrane,and a lower part,a band with *** 4-and 8-cel1 stages,the strip is almost vanishedand microvilli in the band is *** nascentmembrane is smooth at the 180°stage,then a rough areaappears below the smooth region and quickly *** germ agglutinin which can bind to preexistingmembrane interrupts the development of the regionbetween the preexisting and nascent ***,Brij,having the property to increase the areaof nascent membrane,does not interrupt the developmentof the region between the preexisting and nascentmembranes.

关键词： Gleavaqe region between the preexisting and nascent membranes surface active agents wheat germ agglutinin Rana amurensis eggs.

Laser scanning fluorescence microscopic measurement of the movement of cleaving egg surface of Rana Amurensis

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cell Research 1995年第1期5卷 9-24页

作者： gu guoyan (FORMERLY KU KUOYEN) chengtang xu konghua zhang qirong gao.(shanghai institute of cell biology, Chinese Academy of Sciences, shanghai 200031, China) shanghai institute of cell biology Chinese Academy of Sciences Shanghai 200031 China.&#x0002A Dedicated to Prefessor Zhen YAO on the occasion of his 80th birthday.† This work was supported by the director's foundation of shanghai institute of cell biologyReceived 27October1994 Revised 6April1995 Accepted 25April1995

By laser scanning fluorescence microscopy for quan-titative measurement of fluorescence intensity changes on egg surface stained with fluorescein isothiocyanate duxing cleavage furrow extending forward, it was found that in area of presumptive cleavage furrow the scanning curve became ∨ shape, indicating dark stripe appeared in that place. Then the fluorescence intensity increased at the place where the botton of ∨ shape had located, and the scanning curve tuxned to ∧ shape, indicating single stripe was formed. While enhanced fluorescence appeared on the borders of ∧ shape, an M shape curve was found, show-ing double stripe occurred. During the distance between two borders of M shape incresing from 50 μm to 100μm,a fluorescence peak came to sight in the middle of the M shape, which being the cleavge furrow bottom. The two lateral sides of furrow bottom with decreasing fluorescence were nascent membrane. At that time the curve became W shape. By the sides of cleavage furrow the the stress folds became conspicous after double stripe stage, showing the stretching of the egg surface being increased. With our[31, 33] and others[32] reports that polylysine could induce the appearance of nascent membrane and phyto-hemagglutinins could decrease or prevent the appearance of nascent membrane, we believed the idea of Schroeder[25] that increasing mechanical stress could initiate nascent membrane formation and thought that the stress lay to the outsides of cleavage furrow.

关键词： cleavage egg surface movement initiating nascent membrane formation laser scanning fluorescence microscopy Rana Amurensis

Nanopolyphenol rejuvenates microglial surveillance of multiple misfolded proteins through metabolic reprogramming

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Acta Pharmaceutica sinica B 2023年第2期13卷 834-851页

作者： Dayuan Wang Xiao gu Xinyi Ma Jun Chen Qizhi zhang Zhihua Yu Juan Li Meng Hu Xiaofang Tan Yuyun Tang Jianrong xu Minjun xu Qingxiang Song Huahua Song Gan Jiang Zaiming Tang Xiaoling gao hongzhuan Chen Department of Pharmacology and Chemical biology State Key Laboratory of Oncogenes and Related GenesShanghai Universities Collaborative Innovation Center for Translational MedicineShanghai Jiao Tong University School of MedicineShanghai 200025China Key Laboratory of Smart Drug Delivery Ministry of EducationSchool of PharmacyFudan UniversityShanghai 201203China Key Laboratory of cell Differentiation and Apoptosis of Chinese Ministry of Education Shanghai Jiao Tong University School of MedicineShanghai 200025China shanghai Frontiers Science Center of TCM Chemical biology Institute of Interdisciplinary Integrative Medicine ResearchShanghai University of Traditional Chinese MedicineShanghai201203China

Microglial surveillance plays an essential role in clearing misfolded proteins such as amyloid-beta,tau,andα-synuclein aggregates in neurodegenerative ***,due to the complex structure and ambiguous pathogenic species of the misfolded proteins,a universal approach to remove the misfolded proteins remains ***,we found that a polyphenol,α-mangostin,reprogrammed metabolism in the disease-associated microglia through shifting glycolysis to oxidative phosphorylation,which holistically rejuvenated microglial surveillance capacity to enhance microglial phagocytosis and autophagy-mediated degradation of multiple misfolded *** ofα-mangostin efficiently deliveredα-mangostin to microglia,relieved the reactive status and rejuvenated the misfolded-proteins clearance capacity of microglia,which thus impressively relieved the neuropathological changes in both Alzheimer’s disease and Parkinson’s disease model *** findings provide direct evidences for the concept of rejuvenating microglial surveillance of multiple misfolded proteins through metabolic reprogramming,and demonstrate nanoformulatedα-mangostin as a potential and universal therapy against neurodegenerative diseases.

关键词： Microglia Metabolism Misfolded proteins Phagocytosis Degradation Polyphenol Nanoparticles Neurodegenerative diseases

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进展中的原生动物学研究:热点领域与新格局

中国科学：生命科学 2019年第10期49卷 1301-1322页

作者：熊杰陈建平陈晓光陈瑛冯耀宇高凤高珊顾福康黄兵梁爱华龙红岸赖德华伦照荣缪炜倪兵邱子健邵晨汪建国文建凡徐奎栋余育和张龙现张西臣赵元莙宋微波中国科学院水生生物研究所武汉430072 四川大学华西基础医学与法医学院成都610041 南方医科大学公共卫生学院广州510515 哈尔滨师范大学生命科学与技术学院哈尔滨150025 华南农业大学兽医学院广州510642 中国海洋大学海洋生物多样性与进化研究所青岛266003 华东师范大学生命科学学院上海200062 中国农业科学院上海兽医研究所上海200241 山西大学生物技术研究所太原030006 中山大学生命科学学院广州510275 西安交通大学生命科学与技术学院西安710049 中国科学院昆明动物研究所昆明650223 中国科学院海洋研究所青岛266071 河南农业大学牧医工程学院郑州450002 吉林大学动物医学学院长春130062 重庆师范大学生命科学学院重庆市动物生物学重点实验室重庆401331

原生动物是一大类动物性单细胞真核生物.其高度特化的细胞结构与生理特征,独具的进化地位以及与环境、资源、人类健康和动物疾病间的密切关系,特别是其兼具的"细胞"与"动物"这个二元性统一体特性,使得以原生动物为模式或对象的研究在以... 详细信息

原生动物是一大类动物性单细胞真核生物.其高度特化的细胞结构与生理特征,独具的进化地位以及与环境、资源、人类健康和动物疾病间的密切关系,特别是其兼具的"细胞"与"动物"这个二元性统一体特性,使得以原生动物为模式或对象的研究在以细胞学、遗传学、适应与进化为代表的基础生物学、环境生物学、人类的健康与疾病防治、水产养殖及畜牧业等应用学科均具有十分广泛的科学意义和重要的应用价值.半个多世纪以来,伴随着研究队伍的不断壮大和发展,我国的原生动物学研究从早期经典的分类学、寄生虫学、生态学,逐步拓展到今天全面、深入地涉足涵盖基础与应用生物学各学科分支领域.在最近的几十年中,我国聚焦在海洋纤毛虫的多样性与系统学、表观遗传学、细胞生物学、比较基因组学、以寄生原虫为核心的免疫生物学、病害生物学、以鞭毛虫为核心的进化生物学、以海淡水纤毛虫和阿米巴等为核心的原生动物生态学等方向并取得了全面和长足的进展,许多代表性成果处于该领域国际前沿水平.本文扼要陈述了我国原生动物学各主流团队近年来的工作进展,介绍了该领域当前的研究热点和前沿性科学问题,同时对相关领域未来的发展进行了前瞻性描绘和规划.

关键词：原生动物学研究进展多样性与系统学寄生原虫遗传学细胞生物学进化生物学生态学

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