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Determination of Trace Amount of Yttrium with Bromopyrogallol Red by solid-phase Spectrophotometry

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Rare Metals 2001年第3期20卷 202-204页

作者： Jinzhang gao,gang Ni, Li Yuan, Jingwan Kang Department of Chemistry, Northwest Normal University, Lanzhou 730070, China 西北师范大学甘肃兰州 730070

A simple and sensitive method for the determination of trace amount of yttrium by solid-phase spectrophotometry has been studied. Yttrium can form a 1 : 1 complex with bromopyrogallol red (BPR) on resin, which was determined directly at 605 nm, pH=6.5. It has a highly sensitivity (epsilon = 6.3 x 10(6)) which is 300-fold higher than the corresponding spectrophotometry in solution. The method was applied to the determination of yttrium in churchite.

关键词： solid-phase spectrophotometry bromopyrogallol red (BPR) yttrium

Designing hard wear-resistant conductors by introducing high-plasma-energy heterogeneous metals into transition metal nitrides

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Journal of Materials Science & Technology 2023年第26期157卷 213-219页

作者： Yuankai Li Chaoquan Hu Yao Wu Zhenan Qiao Yifan Cheng Zhiqing Gu gang gao Weitao Zheng State Key Laboratory of Superhard Materials Key Laboratory of Automobile Materials of Ministry of EducationSchool of Materials Science and EngineeringJilin Provincial International Cooperation Key Laboratory of High-Efficiency Clean Energy MaterialsJilin UniversityChangchun 130012China State Key Laboratory of Inorganic Synthesis and Preparative Chemistry College of ChemistryJilin UniversityChangchun 130012China SINOPEC Star Co.Ltd. Beijing 100083China Key Laboratory of Medical Electronics and Digital Health of Zhejiang Province College of Information Science and EngineeringJiaxing UniversityJiaxing 314001China National Key Laboratory of Science and Technology on Advanced Composites in Special Environments Harbin Institute of TechnologyHarbin 150080China State Key Laboratory of Automotive Simulation and Control Jilin UniversityChangchun 130025China

*** Hard and wear-resistant conductors(HWCs)refer to functional films with simultaneously high hardness,high wear resistance,and good electrical *** have important applica-tions such as next-generation sliding electrical contacts,high-end conductive probe-tip,and oval hole guides for the precision rolling mill[1-4],which has drawn extensive research ***,it is still challenging to integrate high hardness,super wear resis-tance,and high electrical conductivity in one ***-ally,researchers have mainly alloyed the highly conductive met-als Cu,Au,and Pt to improve their hardness and wear resistance.

关键词： wear high electrical

Surgical treatment of multiple magnet ingestion in children:A singlecenter study

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World Journal of Clinical Cases 2020年第23期8卷 5988-5998页

作者： Duo-Te Cai Qiang Shu Shu-Hao Zhang Jia Liu Zhi-gang gao Department of General Surgery the Children’s Hospital of Zhejiang University School of MedicineHangzhou 310051Zhejiang ProvinceChina Department of General Surgery National Clinical Research Center for Child HealthHangzhou 310051Zhejiang ProvinceChina Department of Pediatric Surgery the Children’s Hospital of Zhejiang University School of MedicineHangzhou 310058Zhejiang ProvinceChina Department of Pediatric Surgery National Clinical Research Center for Child HealthHangzhou 310058Zhejiang ProvinceChina Department of Ear Noseand Throat Surgerythe Children’s Hospital of Zhejiang University School of MedicineHangzhou 310051Zhejiang ProvinceChina

BACKGROUND Since 2017,the number of magnet ingestion cases has increased year over year in our *** all of the ingested magnetic foreign bodies were magnetic beads,and most of the patients experienced intestinal perforations,causing substantial *** To summarize our experience with surgical treatment of multiple magnet ingestion in *** The data for general surgeries were collected from January 2010 to April 2020,and the clinical characteristics,treatment methods,and outcomes were summarized and *** typical cases were selected and *** Fifty-six cases of ingested magnetic foreign bodies were collected,of which 47 were magnetic *** average patient age was 4.7±3.0 years *** number of ingested magnetic foreign bodies ranged from 2 to *** were 26 cases with symptoms at the time of admission,including two cases of *** patients were discharged successfully following conservative treatment and 43 were treated by *** was the main method of *** was used in four cases,of which three were converted to open surgery,and one was treated successfully using surgery through the *** complications occurred in seven cases,incision infections were observed in six,and adhesive ileus was observed in *** Clinicians need to summarize their experiences with treating magnetic foreign body ingestions in detail and carry out clinical research to reduce the damage to children.

关键词： Children Magnetic foreign body Pediatric surgery Intestinal perforation Buckyball Magnetic bead

基于井下煤层水力压裂的微震时空发育规律研究

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煤炭技术 2024年第5期43卷 207-211页

作者：王国举赵立松高刚周金艳河北煤炭科学研究院有限公司河北邢台054000 河北省矿井微震重点实验室河北邢台054000

为了研究井下煤层水力压裂过程中的微震活动规律,以龙凤煤矿首采区1905工作面开展的UDFD瓦斯治理项目为工程背景,采用KJ1073微震监测系统对压裂过程进行实时、动态的监测。监测研究表明:(1)微震事件的发育具有时间效应,发育范围随压裂... 详细信息

为了研究井下煤层水力压裂过程中的微震活动规律,以龙凤煤矿首采区1905工作面开展的UDFD瓦斯治理项目为工程背景,采用KJ1073微震监测系统对压裂过程进行实时、动态的监测。监测研究表明:(1)微震事件的发育具有时间效应,发育范围随压裂时间的增加逐渐扩大,微震事件能量具有周期性,分析与煤岩体裂隙扩展相关:煤岩体弹性压缩→加速扩展→减速扩展→煤岩体弹性压缩的一个循环过程;(2)微震事件发育方向与压裂液体流动的方向、煤体的倾角方向基本一致,发育范围最长为184 m,最短为100 m,垂向集中在9#煤层顶、底板10 m以内;(3)对微震数据进行分类,并采用置信椭圆分析,表明LF7-1、LF7-2压裂孔之间裂隙发育,发生联通,得到了有效的压裂,在后期的瓦斯抽采中得到验证。上述研究探明了压裂裂缝延伸扩展方向和影响范围,对进一步指导现场施工,优化压裂钻孔布置提供依据。

关键词：微震事件瓦斯压裂范围裂隙扩展

Differential Space-Time Expression of StLTPbl Gene Between Resistant and Susceptible Potato Genotypes in Response to Ralstonia solanacearum

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Agricultural Sciences in China 2008年第6期7卷 713-725页

作者： gao gang JIN Li-ping XIE Kai-yun QU Dong-yu Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences Beijing 100081 P.R.China College of Life Science Shanxi Normal University Linfen 041004 P.R.China

This study is to investigate the role of lipid transfer protein （LTP1） gene of potato （Solanum tuberosum） in bacterial wilt （Ralstonia solanacearum） resistance. A novel cDNA clone encoding nsLTP was isolated from cultivated potato （Solanum tuberosum） infected with R. solanacearum by 5＇-rapid amplification of cDNA ends （RACE）. The temporal and spatial expression of StLTPbl was studied during the early stages of potato-R, solanacearum interaction by reverse transcriptase PCR （RT-PCR） and Northern blotting. The sequence analysis of the cloned cDNA, named StLTPbl, showed 691 bp which encoded a type 1 nsLTP of 91 amino acids. Construction of a phylogenic tree showed that StLTPbl is well conserved in the coding region with high identity at the amino acid level with other Solanaceae nsLTPs. The temporal and spatial expression of StLTPbl was studied during the early stages of potato-R, solanacearum interaction. StLTPbl transcription is induced faster and transcripts accumulate to higher concentrations in resistant compared with susceptible genotypes by the pathogen. Dominant differences in the pathogen-induced gene expression pattern between the upper and lower leaves and stems were observed within the same genotypes. In situ hybridization results showed that the StLTPbl mRNA was localized in phloem cells of vascular tissues in potato leaf and stem tissues after pathogen infection. Salicylic acid, methyl jasmonate and abscisic acid could induce StLTPbl gene expression without significant difference between the upper and lower tissues. These abiotic elicitors could produce a long-lastingeffect on the StLTPbl during early stages of potato-R, solanacearum interaction. Differential expression of StLTPbl gene between resistance and susceptible potato genotypes in response to R. solanacearum suggests that this gene plays a key role in plant defense mechanisms.

关键词： Solanum tuberosum nsLTP gene expression Ralstonia solanacearum

The practically renewable and highly efficient electrocatalysts derived from a newly-designed Mo_(8)Pt polyoxometalate compound

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Science China Chemistry 2023年第2期66卷 492-499页

作者： Di Yin Yun-Dong Cao Yi Feng Guang-gang gao Hong Liu Lin-Lin Fan Zhen-Hui Kang School of Materials Science and Engineering University of JinanJinan250022China Institute of Functional Nano and Soft Materials(FUNSOM) Jiangsu Key Laboratory for Carbon-based Functional Materials and DevicesSoochow UniversitySuzhou215123China Macao Institute of Materials Science and Engineering(MIMSE) MUST-SUDA Joint Research Center for Advanced Functional MaterialsMacao University of Science and TechnologyTaipaMacao999078China

Platinum(Pt)catalysts are the most practical electrocatalysts;however,their low abundance and high cost severely limit their large-scale *** recovery is a key issue to be resolved before the use of Pt-based electrocatalysts can be broadly ***,the newly designed polyoxometalate[Pt^(II)(NH_(3))_(4)]_(2)[Mo_(8)O26](Mo_(8)Pt)acts as an ideal recycling medium for renewable and highly effective Pt *** from Mo_(8)Pt,by a facile reduction reaction,the formed Pt/Mo_(2)C/Mo_(2)N@C-N electrocatalyst shows superior electrocatalytic activity as compared to commercial Pt/C(20%)under both acidic and alkaline ***,the reaction of Pt/Mo_(2)C/Mo_(2)N@C-N in H_(2)O_(2)solution can regenerate Mo_(8)Pt for Pt *** is also demonstrated that the Pt electrocatalyst can be used for at least five rounds of recycling and regeneration,showing its efficiency and stability as well as its *** work highlights a recycling medium strategy to the new generation of renewable Pt-based catalysts.

关键词： platinum polyoxometalate electrocatalyst recycling-medium renewability

Conditional gene manipulation:Cre-ating a new biological era

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2012年第7期13卷 511-524页

作者： Jian ZHANG Jing ZHAO Wen-jie JIANG Xi-wei SHAN Xiao-mei YANG Jian-gang gao School of Life Science Shandong University Jinan 250100China

To solve the problem of embryonic lethality in conventional gene knockouts, site-specific recombinase （SSR） systems （Cre-loxP, FIp-FRT, and φC31） have been used for tissue-specific gene knockout. With the combination of an SSR system and inducible gene expression systems （tetracycline and tamoxifen）, stage-specific knockout and transgenic expression can be achieved. The application of this ＂SSR＋inducible＂ conditional tool to genomic manipulation can be extended in various ways. Alternatives to conditional gene targeting, such as conditional gene trapping, multipurpose conditional alleles, and conditional gene silencing, have been developed. SSR systems can also be used to construct precise disease models with point mutations and chromosomal abnormalities. With these exciting achievements, we are moving towards a new era in which the whole genome can be manipulated as we wish.

关键词： Site-specific recombinase Gene targeting Gene trapping Inducible systems φC31 system

Induced pluripotent stem cells:origins, applications, and future perspectives

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第12期14卷 1059-1069页

作者： Jing ZHAO Wen-jie JIANG Chen SUN Cong-zhe HOU Xiao-mei YANG Jian-gang gao School of Life Sciences Shandong University

Embryonic stem(ES)cells are widely used for different purposes,including gene targeting,cell therapy,tissue repair,organ regeneration,and so ***,studies and applications of ES cells are hindered by ethical issues regarding cell *** circumvent ethical disputes,great efforts have been taken to generate ES cell-like cells,which are not derived from the inner cell mass of blastocyst-stage *** 2006,Yamanaka et *** reprogrammed mouse embryonic fibroblasts into ES cell-like cells called induced pluripotent stem(iPS)*** one year later,Yamanaka et *** Thomson et *** reprogrammed human somatic cells into iPS *** the first generation of iPS cells,they have now been derived from quite a few different kinds of cell *** particular,the use of peripheral blood facilitates research on iPS cells because of safety,easy availability,and plenty of cell *** iPS cells have been used for cell therapy,disease modeling,and drug *** this review,we describe the generations,applications,potential issues,and future perspectives of iPS cells.

关键词： Induced pluripotent stem cells Origin Peripheral blood cells Application Potential issues

职业照射对测井工人外周血淋巴细胞数量和细胞周期的影响

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中国辐射卫生 2024年第1期33卷 28-32,39页

作者：李卫国高刚潘艳李海亮阮健磊朴春南傅惊虹刘建香中国疾病预防控制中心辐射防护与核安全医学所辐射防护与核应急中国疾病预防控制中心重点实验室北京100088 山东第一医科大学(山东省医学科学院)预防医学科学学院(放射医学研究所) 山东济南250117

目的分析长期低剂量中子-γ辐射对测井工人外周血淋巴细胞的影响。方法通过现场采集血样和问卷调查获取测井公司工人健康信息,采用LiF圆片和CR-39监测测井工人的γ和中子剂量,应用血细胞计数仪检测外周血淋巴细胞计数,应用流式细胞术... 详细信息

目的分析长期低剂量中子-γ辐射对测井工人外周血淋巴细胞的影响。方法通过现场采集血样和问卷调查获取测井公司工人健康信息,采用LiF圆片和CR-39监测测井工人的γ和中子剂量,应用血细胞计数仪检测外周血淋巴细胞计数,应用流式细胞术检测细胞周期及周期相关蛋白的变化情况。结果部分测井工人年受照剂量超过5 mSv。淋巴细胞计数在最小年剂量0~1 mSv组均值为2.45×10^(9)/L,在最大年剂量5~25 mSv组均值为2.08×10^(9)/L,差值比达到15%。与岗前工人相比,测井工人淋巴细胞周期发生G1期阻滞,周期蛋白p21、CDK2表达增加。结论长期低剂量中子-γ辐射可引起人淋巴细胞计数下降,也可引起淋巴细胞周期和周期蛋白表达改变。

关键词：测井工人中子-γ辐射淋巴细胞计数细胞周期周期蛋白