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Hydrophobic protein in colorectal cancer in relation to tumor stages and grades

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World Journal of Gastroenterology 2010年第22期16卷 2754-2763页

作者： lay-Chin Yeoh Chee-Keat Loh Boon-Hui Gooi Manjit Singh lay-harn gam School of Pharmaceutical Sciences Universiti Sains Malaysia Department of Surgery Penang General Hospital

AIM: To identify differentially expressed hydrophobic proteins in colorectal cancer. METHODS: Eighteen pairs of colorectal cancerous tissues in addition to tissues from normal mucosa were analysed. Hydrophobic proteins were extracted from the tissues, separated using 2-D gel electrophoresis and analysed using Liquid Chromatography Tandem Mass Spectrometry (LC/MS/MS). Statistical analysis of the proteins was carried out in order to determine the significance of each protein to colorectal cancer (CRC) and also their relation to CRC stages, grades and patients’ gender. RESULTS: Thirteen differentially expressed proteins which were expressed abundantly in either cancerous or normal tissues were identified. A number of these proteins were found to relate strongly with a particular stage or grade of CRC. In addition, the association of these proteins with patient gender also appeared to be ***: Stomatin-like protein 2 was found to be a promising biomarker for CRC, especially in female patients. The differentially expressed proteins identified were associated with CRC and may act as drug target candidates.

关键词： Biomarker Colorectal cancer Liquid chromatography tandem mass spectrometry Proteomic approach 2D-polyacrylamide gel electrophoresis

Breast cancer and protein biomarkers

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World Journal of Experimental Medicine 2012年第5期2卷 86-91页

作者： lay-harn gam School of Pharmaceutical Sciences Universiti Sains Malaysia 11800 USM Penang Malaysia

Breast cancer is a healthcare concern of women *** procedures being available for diagnosis,prognosis and treatment of breast cancer,researchers are working intensively on the disease in order to improve the life quality of breast cancer *** present,there is no single treatment known to bring a definite cure for breast *** of the possible solutions for combating breast cancer is through identification of reliable protein biomarkers that can be effectively used for early detection,prognosis and treatments of the ***,the task of identification of biomarkers for breast cancer has become the focus of many researchers worldwide.

关键词： Breast cancer Protein Biomarkers Proteomics

Chemometrics of differentially expressed proteins from colorectal cancer patients

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World Journal of Gastroenterology 2011年第16期17卷 2096-2103页

作者： lay-Chin Yeoh Saravanan Dharmaraj Boon-Hui Gooi Manjit Singh lay-harn gam School of Pharmaceutical Sciences Universiti Sains Malaysia Centre for Drug Research Universiti Sains Malaysia Department of Surgery Penang General Hospital

AIM:To evaluate the usefulness of differentially expressed proteins from colorectal cancer (CRC) tissues for differentiating cancer and normal ***:A Proteomic approach was used to identify the differentially expressed proteins between CRC and normal *** proteins were extracted using Tris buffer and thiourea lysis buffer (TLB) for extraction of aqueous soluble and membrane-associated proteins,***,namely principal component analysis (PCA) and linear discriminant analysis (LDA),were used to assess the usefulness of these proteins for identifying the cancerous state of ***:Differentially expressed proteins identified were 37 aqueous soluble proteins in Tris extracts and 24 membrane-associated proteins in TLB *** on the protein spots intensity on 2D-gel images,PCA by applying an eigenvalue > 1 was successfully used to reduce the number of principal components (PCs) into 12 and seven PCs for Tris and TLB extracts,respectively,and subsequently six PCs,respectively from both the extracts were used for *** LDA classification for Tris extract showed 82.7% of original samples were correctly classified,whereas 82.7% were correctly classified for the cross-validated *** LDA for TLB extract showed that 78.8% of original samples and 71.2% of the cross-validated samples were correctly ***:The classification of CRC tissues by PCA and LDA provided a promising distinction between normal and cancer *** methods can possibly be used for identification of potential biomarkers among the differentially expressed proteins identified.

关键词： Colorectal cancer Proteomics Marker protein Principal component analysis Linear discriminant analysis

Analysis of differentially expressed proteins in cancerous and normal colonic tissues

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World Journal of Gastroenterology 2006年第31期12卷 4973-4980页

作者： lay-harn gam Chiuan-Herng Leow Che Nin Man Boon-Hui Gooi Manjit Singh School of Pharmaceutical Sciences Universiti Sains Malaysia 11800 USM Penang Malaysia National Poison Centre Universiti Sains Malaysia 11800 USM Penang Malaysia Department of Surgery Penang General Hospital Penang Malaysia

AIM： To identify and analyze the differentially expressed proteins in normal and cancerous tissues of four patients suffering from colon cancer. METHODS： Colon tissues （normal and cancerous） were homogenized and the proteins were extracted using three protein extraction buffers. The extraction buffers were used in an orderly sequence of increasing extraction strength for proteins with hydrophobic properties. The protein extracts were separated using the SDS-PAGE method and the images were captured and analyzed using Quantity One software. The target protein bands were subjected to in-gel digestion with trypsin and finally analyzed using an ESI-ion trap mass spectrometer. RESULTS： A total of 50 differentially expressed proteins in colonic cancerous and normal tissues were identified. CONCLUSION： Many of the identified proteins have been reported to be involved in the progression of similar or other types of cancers. However, some of the identified proteins have not been reported before. In addition, a number of hypothetical proteins were also identified.

关键词： Colon cancer Tissue specimens Sequentia protein extraction Proteomics