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Effects of antibacterial compounds produced by Saccharomyces cerevisiae in Koumiss on pathogenic Escherichia coli O8 and its cell surface characteristics

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Journal of Integrative Agriculture 2017年第3期16卷 742-748页

作者： CHEN Yu-jie WANG Chun-jie HOU Wen-qian WANG Xiao-shuo gali bing-ga HUASAI Si-mu-jide YANG Si-qin WU A-qi-ma ZHAO Yu-fei WU Ying-ga CHEN Ao-ri-ge-le Vocational and Technical College Inner Mongolia Agricultural University Baotou 014109 P.R. China College of Animal Science Inner Mongolia Agricultural university Hohhot 010018 P.R. China College of Veterinary Medicine Inner Mongolia Agricultural University Hohhot 010018 P.R. China

The effects of antibacterial compounds produced by Saccharomyces cerevisiae in Koumiss on pathogenic Escherichia coli O8 and its cell surface characteristics were *** isolated from Koumiss produced antibacterial compounds which were active against pathogenic *** O8 as determined by the Oxford cup *** aqueous phases from *** at p H=2.0（S2）and pH=8.0（S8）were extracted and tested,*** organic acids of S2 and S8 were determined by high performance liquid chromatography（HPLC）,and the concentrations of killer toxins were determined by enhanced bicinchoninic acid（BCA）Protein Assay *** minimum inhibition concentration（MIC）and the minimum bactericidal concentration（MBC）of S2 and S8 on *** O8 were determined by the broth microdilution *** effects of S2 and S8 on the growth curve of *** O8 were determined by turbidimetry,and the hydrophobicities of *** O8 cell surface were determined using the microbial adhesion to solvents method,the permeation of *** O8 cell membrane were determined by the o-nitrophenyl-β-D-galactoside（ONPG）*** phases at pH 2.0 and 8.0had larger inhibition zones and then S2 and S8 were obtained by *** main component in S2 was citric acid and it was propanoic acid in *** organic acids and killer toxins were also *** the MICs of S2 and S8 on *** O8 were 0.025 g m L^-1,the MBCs were 0.100 and 0.200 g m L^-1,*** normal growth curve of *** O8was S-shaped,however,it changed after addition of S2 and *** O8 was the basic character,and had a relatively hydrophilic *** hydrophobicity of *** O8 cell surface and the permeation of *** O8 cell membrane were increased after adding S2 and *** present study showed that S2 and S8 inhibit the growth of pathogenic *** O8and influence its cell surface characteristics.

关键词： antibacterial compound Saccharomyces cerevisiae Escherichia coil hydrophobicity permeation

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不同熔解曲线分析技术在犬细小病毒基因分型中的比较

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中国兽医杂志 2021年第9期57卷 32-37,42页

作者：嘎利兵嘎锡林塔娜刘志成内蒙古农业大学职业技术学院内蒙古包头014109 锡林郭勒盟生态环境监测站内蒙古锡林浩特026000 广东省农业科学院动物卫生研究所广东广州510640

本试验旨在建立基于聚合酶链式反应(PCR)技术的犬细小病毒2型(CPV-2)2种不同熔解曲线技术基因分型方法,并通过比较其熔解温度差异(△Tm)评价不同方法对犬细小病毒单核苷酸多态性(SNP)位点的分型鉴别能力。结果显示,探针熔解曲线聚合酶... 详细信息

本试验旨在建立基于聚合酶链式反应(PCR)技术的犬细小病毒2型(CPV-2)2种不同熔解曲线技术基因分型方法,并通过比较其熔解温度差异(△Tm)评价不同方法对犬细小病毒单核苷酸多态性(SNP)位点的分型鉴别能力。结果显示,探针熔解曲线聚合酶链式反应(FMCA-PCR)方法中不同SNP位点之间熔解温度差异(△Tm)均大于3℃,而在高分辨率熔解曲线聚合酶链式反应(HRM-PCR)方法中熔解温度差异(△Tm)只有0.2~0.6℃。临床30份CPV-2核酸样本基因分型结果显示,FMCA-PCR方法的准确率为100%;而HRM-PCR方法准确率为96.6%(未形成杂合子前)。虽然HRM-PCR方法中不同SNP位点之间熔解温度差异较小,影响其基因分型结果,但通过杂合子HRM分析后可提高该方法的准确率。本试验结果表明,基于PCR方法的2种不同熔解曲线基因分型技术均具有较好的SNP位点分型鉴别能力,为熔解曲线基因分型技术在兽医临床中的应用提供实际参考依据。

关键词： HRM-PCR FMCA-PCR CPV-2 基因分型熔解温度

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联合应用阿苯达唑和伊维菌素在线虫感染鄂尔多斯细毛羊体内的药动学研究

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动物医学进展 2012年第1期33卷 6-12页

作者：哈斯苏荣嘎利兵嘎乌翠兰宝鲁德潘保良金海内蒙古农业大学兽医学院内蒙古呼和浩特010018 中国农业大学动物医学院北京100094 鄂尔多斯市乌审旗兽医局内蒙古鄂尔多斯017300

为阐明联合应用阿苯达唑(ABZ)和伊维菌素(IVM)在胃肠道线虫感染鄂尔多斯细毛羊体内的药动学互作关系,以感染胃肠道线虫的鄂尔多斯细毛羊为研究对象,比较研究了单独或联合应用阿苯达唑和伊维菌素后的药物动力学特征。通过粪便虫卵检查法... 详细信息

为阐明联合应用阿苯达唑(ABZ)和伊维菌素(IVM)在胃肠道线虫感染鄂尔多斯细毛羊体内的药动学互作关系,以感染胃肠道线虫的鄂尔多斯细毛羊为研究对象,比较研究了单独或联合应用阿苯达唑和伊维菌素后的药物动力学特征。通过粪便虫卵检查法,选取感染胃肠道线虫的鄂尔多斯细毛羊15只,随机分成3组,每组5只。第1组口服给予阿苯达唑(15mg/kg),第2组皮下注射伊维菌素(0.2mg/kg),第3组皮下注射伊维菌素(0.2mg/kg)的同时口服阿苯达唑(15mg/kg)。于给药后不同时间,由颈静脉采集血样,分离血浆,并用高效液相色谱法测定各时间点血浆阿苯达唑、阿苯达唑亚砜、阿苯达唑砜和伊维菌素浓度,并用PK Solution 2.0药物动力学软件计算出各药动学参数。结果表明,联合用药组绵羊血浆伊维菌素峰浓度(Cmax)、药时曲线下面积(AUC)和平均滞留时间(MRT)分别为44.80ng/mL±6.12ng/mL、5 007.46ng.h/mL±1 301.42ng.h/mL和85.47h±5.03h,均显著(P<0.05)小于单独用药组的对应参数值67.62ng/mL±9.06ng/mL、7 125.08ng.h/mL±908.52ng.h/mL和113.39h±9.00h。口服阿苯达唑组绵羊血浆中仅检测到了阿苯达唑砜和阿苯达唑亚砜,而未检测到阿苯达唑母药。联合用药后,除阿苯达唑砜的达峰时间(T max)显著推迟外,阿苯达唑砜和阿苯达唑亚砜的其他各参数之间均无显著性差异。因此,联合应用IVM和ABZ可影响它们在胃肠道线虫感染鄂尔多斯细毛羊体内的药动学特征,且对伊维菌素药动学特征的影响尤为明显,在临床联合用药过程中应予以重视。

关键词：阿苯达唑伊维菌素联合用药药动学相互作用

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Electrocatalytic CO_(2)reduction to syngas

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Green Energy & Environment 2024年第7期9卷 1085-1100页

作者： bing Chang Zhaojun Min Ning Liu Nan Wang Maohong Fan Jing Fan Jianji Wang School of Environment Key Laboratory of Yellow River and Huai River Water Environment and Pollution ControlMinistry of EducationHenan Normal UniversityXinxiangHenan453007China School of Chemistry and Chemical Engineering Key Laboratory of Green Chemical Media and ReactionsMinistry of EducationHenan Normal UniversityXinxiangHenan453007China College of Engineering and Physical Sciences and School of Energy ResourcesUniversity of WyomingLaramieWY82071USA College of Engineering Georgia Institute of TechnologyAtlantaGA30332USA

While carbon dioxide(CO_(2))is a major greenhouse gas,it is also an important C1 *** the trend of energy conservation and emission reduction,electrocatalytic reduction has become a very promising strategy for CO_(2)utilization because it can convert CO_(2)directly to high-valued chemicals and fuels under mild *** particular,the product CO and by-product H_(2)can be combined into syngas by an electrocatalytic CO_(2)reduction reaction(CO_(2)RR)in an aqueous *** molar ratios of CO and H_(2)may be used to produce essential bulk chemicals or liquid fuels such as methanol,alkanes,and olefins through thermochemical catalysis,Fischer-Tropsch synthesis,microbial fermentation,and other *** work discusses the latest strategies in controlling the molar ratio of CO/H_(2)and improving the yield of CO_(2)*** challenges of electrocatalytic syngas production are analyzed from an industrial application perspective,and the possible measures to overcome them are proposed in terms of new catalyst design,electrolyte innovation,flow reactor optimization,anodic reaction coupling,and operando technique application.

关键词： Electrocatalysis CO_(2)reduction Syngas Electrolyte Electrolyzer

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Damping capacity of Fe_(83)ga_(17) magnetostrictive alloy under magnetic field

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Rare Metals 2024年第1期43卷 402-409页

作者： Shao-Wen Yan Xing Mu bing-Jie Liu Li-Hong Xu Shi-Hai Guo Yan Qi Hong-Ping Zhang Department of Functional Material Research Central Iron and Steel Research InstituteBeijing 100081China

The damping performance of Fe_(83)ga_(17) alloys has been investigated in the static magnetic field (H). The results demonstrate that the damping peak value can be adjusted between 0.020 and 0.085 under the magnetic f... 详细信息

关键词： alloy magnetic alloys

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条带状铁建造消失之谜:铁-硅互层机制研究进展与展望

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地学前缘 2024年第1期31卷 111-126页

作者：王瑞敏沈冰北京大学地球与空间科学学院造山带与地壳演化教育部重点实验室北京100871

条带状铁建造(banded iron formation, BIF)是指以铁-硅韵律层结构为特征的化学沉积岩。BIF不仅是重要的铁资源,也是记录早期地球演化、早期微生物活动和大气-海洋氧化还原状态的重要载体。BIF的出现和消失与重要环境事件的演变密切相关... 详细信息

条带状铁建造(banded iron formation, BIF)是指以铁-硅韵律层结构为特征的化学沉积岩。BIF不仅是重要的铁资源,也是记录早期地球演化、早期微生物活动和大气-海洋氧化还原状态的重要载体。BIF的出现和消失与重要环境事件的演变密切相关。BIF在>3.8 ga首次出现,并在第一次大氧化事件前大量形成,在1.8~0.8 ga沉积中断,而在成冰纪雪球地球冰期中复现,最终消失在显生宙的氧化海洋中。传统模型认为BIF的出现与消失主要受控于海洋中Fe(Ⅱ)离子的含量。在1.8~0.8 ga期间,构造-岩浆-热液活动减弱,海洋氧化还原剧变(氧化/硫化)导致海水中的Fe(Ⅱ)被大量耗尽,BIF消失。然而,地球化学证据表明中元古代深海区域仍处于铁化状态,即Fe(Ⅱ)可以稳定存在的氧化还原状态;沉积学证据也表明中元古代仍有大量的铁岩沉积。这些富铁沉积的大量出现,表明中元古代的海洋仍具有沉积铁岩/富铁层的条件,只是不再出现BIF这种“铁-硅互层”的韵律沉积。因此,BIF在1.8~0.8 ga的沉积中断并不是由Fe(Ⅱ)耗尽造成的,而很可能与海水中铁-硅循环变化导致“铁-硅互层”沉积模式的改变有关。本文对BIF的地质学特征进行了概述,深入探讨了BIF中富铁-富硅层的沉积模式,并从Fe(Ⅱ)的来源差异和Fe(Ⅱ)的氧化差异及Fe(Ⅲ)的保存差异3方面全面综述了BIF “铁-硅互层”机制的研究进展。在此基础上,尝试找出解决中元古代BIF消失之谜的可能方向。最后,对BIF的成因机制所承载的前寒武纪铁-硅生物地球化学循环、早期地球演化过程和早期生命信息等方面提出展望。

关键词：条带状铁建造铁循环硅循环韵律层沉积前寒武纪

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Overexpression of Pbrga2ox1 enhances pear drought tolerance through the regulation of ga_(3)-inhibited reactive oxygen species detoxification and abscisic acid signaling

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Journal of Integrative Agriculture 2024年第9期23卷 2989-3011页

作者： Guoling Guo Haiyan Zhang Weiyu Dong Bo Xu Youyu Wang Qingchen Zhao Lun Liu Xiaomei Tang Li Liu Zhenfeng Ye Wei Heng Liwu Zhu bing Jia School of Horticulture Anhui Agricultural UniversityHefei 230036China Anhui Wanbang Pharmaceutical Technology Co. Ltd.Hefei 230036China Science and Technology Department Anhui Agricultural UniversityHefei 230036China Hunan Suncas Agriculture Science Co. Ltd.Changsha 410016China

Drought stress is a devastating natural disaster driven by the continuing intensification of global warming,which seriously threatens the productivity and quality of several horticultural crops,including ***(gas)play crucial roles in plant growth,development,and responses to drought *** studies have shown significant reductions of ga levels in plants under drought stress;however,our understanding of the intrinsic regulation mechanisms of ga-mediated drought stress in pear remains very ***,we show that drought stress can impair the accumulation of bioactive gas(Bgas),and subsequently identified Pbrga2ox1 as a chloroplast-localized ga deactivation *** gene was significantly induced by drought stress and abscisic acid(ABA)treatment,but was suppressed by ga_(3)***2ox1-overexpressing transgenic tobacco plants(Nicotiana benthamiana)exhibited enhanced tolerance to dehydration and drought stresses,whereas knock-down of Pbrga2ox1 in pear(Pyrus betulaefolia)by virus-induced gene silencing led to elevated drought *** plants were hypersensitive to ABA,and had a lower Bgas content,enhanced reactive oxygen species(ROS)scavenging ability,and augmented ABA accumulation and signaling under drought stress compared to wild-type ***,the opposite effects were observed with Pbrga2ox1 silencing in ***,exogenous ga_(3)treatment aggravated the ROS toxic effect and restrained ABA synthesis and signaling,resulting in the compromised drought tolerance of *** summary,our results shed light on the mechanism by which Bgas are eliminated in pear leaves under drought stress,providing further insights into the mechanism regulating the effects of ga on the drought tolerance of plants.

关键词： pear gibberellin drought stress Pbrga2ox1 reactive oxygen species abscisic acid

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Eu-MOF-Based Fluorescent Ratiometric Sensor by Detecting 3,4,5-Trihydroxybenzoic for Fingerprint Visualization on Porous Objects

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Photonic Sensors 2024年第1期14卷 85-98页

作者： Muwen LIANG Yabin ZHAO Yaping LUO Bin DU Wei HU bing LIU Xihui MU Zhaoyang TONG Department of Investigation People's Public Security University of ChinaBejing 100038China State Key Laboratory of NBC Protection for Civilian Beijing 102205China

Latent fingerprints(LFPs)at the crime scene are served as important clues to locate the trajectory of criminal behavior and portray the characteristics of the ***,visualizing LFPs is of considerable *** this work,the europium metal-organic framework(Eu-MOF)sensor was successfully constructed for sensitive detection of gallic acid(3,4,5-trihydroxybenzoic acid,ga)and visualization of the sweat *** boric-acid-modified Eu-MOF was prepared by using the simple one-pot solvothermal method using Eu as the metal i ion center and 3,5-dicarboxybenzeneboronic acid(BBDC)as the organic *** sensor showed desirable photoluminescent performance through the chelating of BBDC with Eu3+.The sensor exhibited the satisfactory linear relationship to ga in the range of 1 nM to 20 nM with a low detection limit of 0.34 nM under the optimized *** prepared sensor with ideal selectivity to ga was successfully applied for visualizing LFPs on porous substrates with the high contrast and superior *** the good performance of the sensor,all fingerprint images obtained from 1200 samples presented clear friction ridges and met the identification ***,the sensor had less impact on the subsequent deoxyribonucleic acid(DNA)detection,displaying a promising perspective for applications in extracting physical evidence of site investigation.

关键词： Europium metal-organic framework(Eu-MOF) photoluminescence 3,4,5-trihydroxybenzoic latent fingerprints(LFPs)development

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Analysis of combustion instability via constant volume combustion in a LOX/RP-1 bipropellant liquid rocket engine

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Science China(Technological Sciences) 2012年第4期55卷 1066-1077页

作者： ZHANG HuiQiang ga YongJing WANG bing WANG XiLin Department of Engineering Mechanics Tsinghua UniversityBeijing 100084China

Turbulent two-phase reacting flow in the chamber of LOX/RP-1 bipropellant liquid rocket engine is numerically investigated in this paper. The predicted pressure and mean axial velocity are qualitatively consistent with the experimental measurements. The self-excited pressure oscillations are obtained without any disturbance introduced through the initial and boundary conditions. It is found that amount of abrupt pressure peaks appear frequently and stochastically in the head regions of the chamber, which are the important sources to drive and strengthen combustion instability. Such abrupt pressures are induced by local constant volume combustion, because local combustible gas mixtures with high temperature are formed and burnt out suddenly due to some fuel droplets reaching their critical state in a rich oxygen surrounding. A third Damkhler number is defined as the ratio of the characteristic time of a chemical reaction to the characteristic time of a pressure wave expansion to measure the relative intensity of acoustic propagation and combustion process in thrusters. The analysis of the third Damkhler number distributions in the whole thrust chamber shows that local constant volume combustion happens in the head regions, while constant pressure combustion presents in the downstream regions. It is found that the combustion instability occurs in the head regions within about 30 mm from the thruster head.

关键词： combustion instability constant volume combustion spray combustion LOX/RP-1 bipropellant liquid rocket engine third Damkohler number

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低温及赤霉素对八仙花盆花花期调控技术的影响

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北方园艺 2024年第1期 67-75页

作者：蒋心笛赵冰西北农林科技大学风景园林艺术学院陕西杨凌712100

以八仙花‘无尽夏’为试材,通过4℃低温调控及不同浓度和不同喷施间隔的激素(ga3)处理,观察分析各处理的花期、开花时长变化及统计平均花序数和开花时长,研究了温度及赤霉素对八仙花花期的影响,以期在保证开花品质的前提下调控八仙花花... 详细信息

以八仙花‘无尽夏’为试材,通过4℃低温调控及不同浓度和不同喷施间隔的激素(ga3)处理,观察分析各处理的花期、开花时长变化及统计平均花序数和开花时长,研究了温度及赤霉素对八仙花花期的影响,以期在保证开花品质的前提下调控八仙花花期于春节乃至元旦前后,探究最优促花模式。结果表明:在4℃条件下低温5、6、7周均可提前八仙花盆花花期,且随低温时长的增加植株生长越好。其中,低温7周后进行加温催花仅需81 d即可到达初花期,较自然低温花期提前51 d,且可观花天数持续82 d左右,但低温时长较短时开花天数相对缩短、观花效果不佳。较单一低温处理,结合适宜浓度赤霉素的喷施可增强促花效果,进一步提前花期,但过高过低浓度易造成花序伸长畸形或推迟花期。低温6周后连续3次间隔7 d进行喷施浓度为7.5 mg·kg^(-1)的赤霉素,促花效果最好,可于1月20日左右进入初花期,观花长达60 d,同时整体植株具有更好的观赏品质。若想进一步提前花期可在低温7周后进行赤霉素的喷施。筛选所得最佳调控组合,可供生产中利用低温诱导和外源赤霉素调控八仙花盆花花期并提高催花质量,为盆栽八仙花花期调控技术及机理研究提供参考依据。

关键词：八仙花花期调控开花低温赤霉素

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