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Carvacrol Promotes Cell Cycle Arrest and Apoptosis through PI3K/AKT Signaling Pathway in MCF-7 Breast Cancer Cells

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Chinese Journal of Integrative Medicine 2021年第9期27卷 680-687页

作者： Ashok Mari gopikrishnan mani Sirpu Natesh Nagabhishek gopaiakrishnan Balaraman Nirmala subramanian Fathima Bushra Mirza Jagan Sundaram Devaki Thiruvengadam Department of Biochemistry University of MadrasGuindy CampusChennai(600025)India Cancer Biology Lab Department of Nanoscience and NanotechnologySathyabama Institute of Science and TechnologyChennai(600119)India VRR Institute of Biomedical Science KattupakkamChennai(600056)India

Objective:To examine the role of carvacrol in modulating PI3 K/AKT signaling involved in human breast cancer pathogenesis using in vitro experimental model MCF-7 ***:MTT and lactate dehydrogenase assays were performed with cells treated with different doses of carvacrol(0–250μmol/L)at different time points(24 and 48 h).The nuclear morphology was assessed in MCF-7 cells with propidium iodide(PI)and acridine orange/ethidium bromide(AO/EB)staining and analyzed by fluorescence *** like cell cycle arrest and apoptosis were observed by flow cytometric analysis and expressions of p-Rb,cyclin D1,cyclin-dependent kinase 4(CDK4),CDK6,Bax,Bcl-2,PI3 K/p-AKT were analyzed by ***:Carvacrol significantly reduced cell viability with the half maximal inhibitory concentration value of 200μmol/L at 24 and 48 h(Pgnificant increase in the accumulation of the g0/g1 phase upon treatment with carvacrol in MCF-7 cells(Pgnificantly inhibited PI3 K/p-AKT protein expressions leading to induction of apoptosis mediated by decreased Bcl2 and increased Bax protein ***,Annexin V/PI staining by FACS analysis,dual staining by AO/EB and PI staining studies suggested induction of apoptosis by carvacrol through PI3 K/Akt signaling pathway in MCF-7 ***:Carvacrol significantly inhibited the breast cancer MCF-7 cell proliferation and induced apoptosis via suppressing PI3/AKT signaling pathway.

关键词： carvacrol breast cancer proliferation apoptosis PI3K/AKT signaling pathway

高Nb X80管线钢焊接热影响区显微组织与韧性

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金属学报 2010年第5期46卷 541-546页

作者：缪成亮尚成嘉王学敏张龙飞 mani subramanian 北京科技大学材料科学与工程学院北京100083 Department of Materials and Engineering McMaster University

利用gleeble-1500模拟不同热输入的单道次焊接热循环过程,通过光学显微镜(OM)、扫描电镜(SEM)及电子背散射衍射(EBSD)对高Nb X80管线钢焊接热影响区中的粗晶区(CgHAZ)显微组织、马/奥组元(M/A)形态和分布以及原奥氏体晶粒粗化情况进行研... 详细信息

利用gleeble-1500模拟不同热输入的单道次焊接热循环过程,通过光学显微镜(OM)、扫描电镜(SEM)及电子背散射衍射(EBSD)对高Nb X80管线钢焊接热影响区中的粗晶区(CgHAZ)显微组织、马/奥组元(M/A)形态和分布以及原奥氏体晶粒粗化情况进行研究.结果表明:高Nb X80管线钢的焊接热输入应不高于30 kJ/cm,虽然在高Nb钢中,原奥氏体晶粒的平均尺寸不会随热输入增加过于粗大,但当热输入高于40 kJ/cm时,会使得原奥氏体晶粒内粒状贝氏体的晶体取向选择过于单一,大角晶界(≥15°)密度明显降低,有效晶粒尺寸较大,M/A组元也由于热输入过大而明显粗化.上述原因使高Nb X80管线钢在大于40 kJ/cm热输入条件下热影响区粗晶区韧性明显恶化.

关键词：高Nb X80管线钢热输入热影响区韧性马氏体/奥氏体

FibroSURE^(TM) and FibroScan~ in relation to treatment response in chronic hepatitis C virus

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World Journal of gastroenterology 2011年第41期17卷 4581-4589页

作者： Keyur Patel Mireen Friedrich-Rust Yoav Lurie Mircea grigorescu Carol Stanciu Chuan-Mo Lee Eugene R Schiff Dieter Hussinger Michael P Manns guido gerken Isabelle Colle Michael Torbenson Erik Pulkstenis g.mani subramanian John g McHutchison Stefan Zeuzem Department of gastroenterology and Hepatology Duke Clinical Research Institute Department of gastroenterology and Hepatology J.W.Goethe University Hospital Department of gastroenterology and Hepatology Tel Aviv Sourasky Medical Center Department of Clinical Infectious Disease Spitalul Clinic de Urgenta Department of gastroenterology and Hepatology Institutul de Gastroenterologie si Hepatologie Department of gastroenterology Chang Gung Memorial Hospital-Kaohsiung Medical CenterChang Gung University College of Medicine Departments of gastroenterology and Internal Medicine University of Miami Department of gastroenterology and Hepatology Universittsklinik Department of gastroenterology and Hepatology Department of gastroenterology and Hepatology Ghent University Hospital Department of Pathology Johns Hopkins University School of Medicine Department of Biostatistics Human Genome Sciences Department of Clinical Research Human Genome Sciences

AIM:To compare histological endpoint assessment using noninvasive alternatives to biopsy during treatment in a chronic hepatitis C virus(HCV)***:Patients with chronic HCV were randomized to receive interferon-based therapy for 24(genotypes 2/3)or 48(genotype 1)***～TM(FS)was assessed at baseline and at week-12 post-treatment *** biopsy for METAVIR was assessed by a single ***～ transient elastogra-phy(TE)was performed during treatment in a patient ***:Two thousand and sixty patients(n = 253 in Asia)were classif ied as METAVIR F0-1(n = 1682)or F2-4(n = 378).For F2-4,FS(n = 2055)had sensitiv-ity and specif icity of 0.87 and 0.61,respectively,with area under the receiver-operating curve of 0.82;corre-sponding values for TE(n = 214)and combined FS/TE(n = 209)were 0.77,0.88 and 0.88,and 0.93,0.68 and *** FS/TE agreement for F2-4 was 71%(κ = 0.41)and higher in Asians vs non-Asians(κ = 0.86 vs 0.35;P gical response than in nonresponders,and were maintained through ***:FS and TE may reliably differentiate mild from moderate-advanced disease,with a potential for high diagnostic accuracy in Asians with chronic HCV.

关键词：丙型肝炎病毒治疗慢性 TM 评估基准亚洲人工作曲线 TE

奥氏体变形及冷却速率对低碳贝氏体组织中大角晶界分布的影响

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北京科技大学学报 2012年第3期34卷 289-297页

作者：缪成亮尚成嘉 mani subramanian 北京科技大学材料科学与工程学院北京100083 麦克马斯特大学材料与工程学院加拿大哈密尔顿L8S4M1

使用电子背散射衍射技术研究了低C高Mn高Nb成分设计下,非再结晶奥氏体变形及加速冷却速率对低碳贝氏体组织取向差特征和大角晶界分布的影响.结果表明,与原奥氏体晶粒内部的相变组织相比,原奥氏体晶界附近具有更高的大角晶界密度,非再结... 详细信息

使用电子背散射衍射技术研究了低C高Mn高Nb成分设计下,非再结晶奥氏体变形及加速冷却速率对低碳贝氏体组织取向差特征和大角晶界分布的影响.结果表明,与原奥氏体晶粒内部的相变组织相比,原奥氏体晶界附近具有更高的大角晶界密度,非再结晶区奥氏体变形及快速冷却都有利于提高共格相变的驱动力、弱化变体选择以及有效增加大角晶界密度.此外,非再结晶区的大变形除了可充分压扁奥氏体晶粒和增加单位面积的奥氏体晶界密度外,还导致奥氏体晶界上细小的非共格转变铁素体晶粒生成,且这些铁素体晶粒与相邻组织表现出大取向差.

关键词：管线钢晶界冷却奥氏体形变贝氏体

Development of Haloalkane Dehalogenases for Industrial Products

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Development of Haloalkane Dehalogenases for Industrial Produ...

The Seventh China-Japan Joint Symposium on Enzyme Engineering

作者： Kai Li mani subramanian Paul Swanson Joeseph Krebs Kevin gray Toby Richardson Keith Kretz Dan Robertson The Dow Chemical Company

Commercial manufacture of propylene oxide and epichlorohydrin（epi） by chlorination and hydration of propylene,results in the production of 1,2,3-trichloropropane（TCP）,an undesirable side product,hi order to recover halohydrin from TCP and recycle into the epi process,a Rhodococcus rhodochrous slrain was isolated by cblorocarbon-enrichrnent technique.A haloalkane dehalogenase was purified from this bacteria which catalyzed the hydrolytic dehalogenation of TCP to dichlorohalohydrin（DCH）.However,the activity of mis enzyme was too low for recovery of DCH from TCP（turnover of 0.15/second）.In addition,the enzyme was competitively inhibited by *** different Directed Evolution methods were applied to the dehalogenase gene in order to improve the *** resulted in obtaining a thermostable enzyme as well as a 15-fold improvement of catalytic ***,biopanning of DNA-libraries from 576 different environmental samples（from tropical jungles,arid deserts,agricultural fields,contaminated waste sites,sediments from lakes,rivers,streams and marine environments） yielded25-full length and 27 partial *** analysis of the sequences revealed relationship spread across all seven known *** of the newly discovered genes were shown to have different properties such as:（a） Improved catalytic activity *** relative to the wild type enzyme（b）Activity against different analogs of TCP（c） Much reduced feedback inhibition by DCH and（d） Different *** of biopanning and Directed Evolution is a powerful tool for rapid discovery new genes and improvement of enzyme functions for production of industrial products from various starting materials.

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从富马酸替诺福韦二吡呋酯(TDF)转换为磷丙替诺福韦(TAF)后骨骼和肾脏安全性改善:两项对HBeAg阳性和HBeAg阴性的慢性乙型肝炎(CHB)患者的3期研究的初步结果

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从富马酸替诺福韦二吡呋酯(TDF)转换为磷丙替诺福韦(TAF)后骨骼和...

第九届全国疑难及重症肝病大会

作者： Henry L.Chan Scott Fung Wai Kay Seto Ed gane John F.Flaherty Vithika Suri Lanjia Lin Anuj gaggar g.mani subramanian Wan Long Chuang Kosh Agarwal Harry L.Janssen Maria Buti The Chinese University of Hong Kong Toronto general Hospital Queen Mary Hospital Hong Kong Kaohsiung Medical University Hospital Kings College Hospital Toronto Western Hospital Erasmus Medical Center

背景和目的:两项大型多中心3期研究中,在48周治疗后,相比于TDF,TAF治疗对骨骼和肾脏的影响更小,且疗效率相似。本文档评估了完成96周双盲TAF或TDF治疗且换为开放性标签TAF治疗的患者,以确定骨密度、肌酐清除率的变化以及病毒抑制的维持...

背景和目的:两项大型多中心3期研究中,在48周治疗后,相比于TDF,TAF治疗对骨骼和肾脏的影响更小,且疗效率相似。本文档评估了完成96周双盲TAF或TDF治疗且换为开放性标签TAF治疗的患者,以确定骨密度、肌酐清除率的变化以及病毒抑制的维持情况。方法:两项设计相同的3期研究中,HBeAg阴性(gS-US-320-0108;N=425)或HBeAg阳性

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一项在HBeAg阳性、慢性乙型肝炎(CHB)患者中比较磷丙替诺福韦(TAF)与富马酸替诺福韦二吡呋酯(TDF)的3期研究:96周时疗效和安全性结果

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一项在HBeAg阳性、慢性乙型肝炎(CHB)患者中比较磷丙替诺福韦(TAF...

第九届全国疑难及重症肝病大会

作者： Kosh Agarwal Scott Fung Wai Kay Seto Young Suk Lim Ed gane Harry L.Janssen Manoj Sharma Wan Long Chuang Ho Bae Ki Tae Yoon John F.Flaherty Audrey Lau Anuj gaggar Vithika Suri Andrea Cathcart Lanjia Lin g.mani subramanian Shalimar Norihiro Furusyo Maria Buti Henry L.Chan Kings College Hospital Department of Medicine University of Toronto Queen Mary Hospital Hong Kong Asan Medical Center Kaohsiung Medical University Hospital Pusan National University Yangsan Hospital All India Institute of Medical Sciences Kyushu Medical Center The Chinese University of Hong Kong

背景和目的:TAF是替诺福韦(TFV)的一种新型前体药,在血浆中比TDF更稳定,增强了TFV进入肝细胞的给药,且循环TFV水平降低。这项针对HBeAg阳性患者的随机双盲研究显示,之前在48周时,在HBV DNA小于29IU/mL的患者比例方面,TAF的疗效并不劣于T...

背景和目的:TAF是替诺福韦(TFV)的一种新型前体药,在血浆中比TDF更稳定,增强了TFV进入肝细胞的给药,且循环TFV水平降低。这项针对HBeAg阳性患者的随机双盲研究显示,之前在48周时,在HBV DNA小于29IU/mL的患者比例方面,TAF的疗效并不劣于TDF,且骨骼和肾脏影响改善。本文摘展示了96周治疗后的结果。

关键词：

病毒性肝炎:乙型肝炎和丁型肝炎-临床(治疗、新化合物、耐药性) 一项在HBeAg阴性、慢性乙型肝炎(CHB)患者中比较磷丙替诺福韦(TAF)与富马酸替诺福韦二吡呋酯(TDF)的3期研究:96周时疗效和安全性结果

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病毒性肝炎:乙型肝炎和丁型肝炎-临床(治疗、新化合物、耐药性) ...

第九届全国疑难及重症肝病大会

作者： Maurizia Brunetto Young Suk Lim Ed gane Wai Kay Seto Marina Osipenko Sang Hoon Ahn Harry L.Janssen Akash Shukla Wan Long Chuang Huy Trinh Mustafa K.Celen John F.Flaherty Audrey H.Lau Anuj gaggar Vithika Suri Neeru Bhardwaj Kyungpil Kim g.mani subramanian Calvin Pan Namiki Izumi Patrick Marcellin Henry Chan Maria Buti University Hospital of Pisa Asan Medical Centre Erasmus Medical Center

背景和目的:TAF是替诺福韦(TFV)的一种新型前体药,在血浆中比TDF更稳定,增强了TFV进入肝细胞的给药,且循环TFV水平降低。这项在HBeAg阴性患者中比较TAF与TDF的随机双盲研究显示,之前在48周时,在HBV DNA小于29IU/mL的患者比例方面,TAF的...

背景和目的:TAF是替诺福韦(TFV)的一种新型前体药,在血浆中比TDF更稳定,增强了TFV进入肝细胞的给药,且循环TFV水平降低。这项在HBeAg阴性患者中比较TAF与TDF的随机双盲研究显示,之前在48周时,在HBV DNA小于29IU/mL的患者比例方面,TAF的疗效并不劣于TDF,且骨骼和肾脏影响改善。本文摘展示了96周治疗后的结果。方法:425例患者被随机分配到接受TAF 25mg QD(n=285)或TDF 300mg QD(n=140),且治疗144

关键词：

一项对比从富马酸替诺福韦二吡呋酯(TDF)转换为丙酚替诺福韦(TAF)和继续TDF治疗用于病毒学抑制的慢性乙型肝炎(CHB)患者的3期研究:第48周时的疗效和安全性结果

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一项对比从富马酸替诺福韦二吡呋酯(TDF)转换为丙酚替诺福韦(TAF)...

第十届全国疑难及重症肝病大会

作者： Pietro Lampertico Maria Buti Scott Fung Sang Hoon Ahn Wan-Long Chuang Won Young Tak Alnoor Ramji Chi-Yi Chen Edward Tam Ho Bae Xiaoli Ma John F.Flaherty Anuj gaggar Audrey Lau Becket Feierbach george Wu Vithika Suri mani subramanian Cong Cheng Huy N.Trinh Seung Kew Yoon Kosh Agarwal Young-Suk Lim Henry Chan Università degli Studi di Milano Division of Gastroenterology and Hepatology University Hospital Vall d'Hebron University Health Network Yonsei University Kaohsiung Medical University

背景和目的TAF是替诺福韦(TFV)的一种新型前体药物,近期获批用于治疗慢性乙型肝炎。与TDF相比,TAF的血浆稳定性更高,能够更有针对性地将TFV递送至肝脏且降低了TFV的循环水平。第48周和第96周时,TAF表现出不劣于TDF的疗效且改善肾脏和骨...

背景和目的TAF是替诺福韦(TFV)的一种新型前体药物,近期获批用于治疗慢性乙型肝炎。与TDF相比,TAF的血浆稳定性更高,能够更有针对性地将TFV递送至肝脏且降低了TFV的循环水平。第48周和第96周时,TAF表现出不劣于TDF的疗效且改善肾脏和骨骼安全性。本研究评估了从TDF转换为TAF vs.继续接受1年TDF治疗用于病情稳定的病毒学抑制患者时的疗效和安全性。

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