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miR147b.白细胞介素-1β介导的人类星形胶质细胞炎症中的新型关键调节因子

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神经损伤与功能重建 2018年第12期13卷 662-662页

作者： van Schepping.n J Mills JD Zimmer TS b.oekaart DWM Iori v b.ng.arts A Anink JJ Iyer AM Korotkov A Jansen FE van Hecke W Spliet Wg.van Rijen PC b.ayen JC vezzani A van vliet EA Aronica E 聂昊 Department of (Neuro)Patholog. Academic Medical Center University of Amsterdam Department of Neuroscience IRCCS-Istituto di Ricerche Farmacologiche "Mario Negri" Department of Pediatric Neurolog. University Medical Center Utrecht Department of Patholog. University Medical Center Utrecht Department of Neurosurg.ry Rudolf Magnus Institute for NeuroscienceUniversity Medical Center Utrecht Department of Neurosurg.ry VU University Medical Center Stichting.Epilepsie Instelling.n Nederland (SEIN)

星形胶质细胞是大脑炎症过程的重要介质,可能在包括癫痫在内的几种神经系统疾病中起重要作用。星形胶质细胞中可生产几种miRNA,是炎症通路的关键调节因子,还可能用作治疗靶标。本研究将探索在体外IL-1β介导的炎症条件下,星形胶质细胞... 详细信息

星形胶质细胞是大脑炎症过程的重要介质,可能在包括癫痫在内的几种神经系统疾病中起重要作用。星形胶质细胞中可生产几种miRNA,是炎症通路的关键调节因子,还可能用作治疗靶标。本研究将探索在体外IL-1β介导的炎症条件下,星形胶质细胞内生成的miRNA及其功能,并在人癫痫脑组织中验证。我们通过测序评估IL-1β刺激的人胎儿星形胶质细胞培养物中的miRNA和mRNA表达。使用miRNA模拟物在细胞培养物中过表达miRNA。使用原位杂交技术检测患有结节性硬化症复合体或颞叶癫痫伴海马硬化患者的切除脑组织中miRNA的表达。我们发现了2种差异表达的miRNA:miR146a和miR147b.它们与免疫/炎症反应相关基因的表达增加有关。既往有研究报道,在星形胶质细胞和结节性硬化复合细胞培养物中使用IL-1β刺激后,miR147b.过表达降低了促炎介质IL-6和COX-2的表达。miR146a和miR147b.表达降低了星形胶质细胞的增殖并促进人神经干细胞的神经元分化。miR147b.致癫痫脑中的星形胶质细胞中表达增加。由于其抗炎和恢复异常星形细胞增殖和促进神经元分化的能力,miR146a和miR147b.为与炎症相关的神经疾病(例如癫痫)的潜在治疗靶标值得进一步研究。

关键词：星形胶质细胞炎症白细胞介素1-β 微小RNA 颞叶癫痫结节性硬化症

三江源及其毗邻地区公里网格降水数据集(2009-2013)

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全球变化数据学报（中英文） 2020年第2期4卷 163-169,163-169页

作者：蒋育昊李宝林袁烨城高锡章张涛刘岩李影罗智勇李红马强王晓敏次仁多吉中国科学院地理科学与资源研究所资源与环境信息系统国家重点实验室北京100101 中国科学院大学北京100049 青海省黄南藏族自治州河南蒙古族自治县气象局黄南811599 西藏自治区拉萨市气象局拉萨850000

三江源及其毗邻地区公里网格降水数据集(2009-2013)是对2009-2013年的TRMM3b.3v7降水数据进行降尺度后生成的该区域降水数据及其相关对照或辅助数据。其中,降尺度后的TRMM数据是基于山地降水二次抛物型廓线(Quadratic Parab.lic Profile... 详细信息

三江源及其毗邻地区公里网格降水数据集(2009-2013)是对2009-2013年的TRMM3b.3v7降水数据进行降尺度后生成的该区域降水数据及其相关对照或辅助数据。其中,降尺度后的TRMM数据是基于山地降水二次抛物型廓线(Quadratic Parab.lic Profile,QPP)模型生成的。该方法包括两个步骤:(1)推算QPP模型参数。三江源及其毗邻地区的最大降水海拔和NDvI峰值海拔高度是对应的,因此,利用NDvI峰值所在空间位置来确定最大降水海拔,进而基于最大降水海拔推算该位置的降水量和降水抛物线方程的二次项系数。(2)模型参数空间外推。利用反距离权重(IDW)插值法对0.25°尺度参数进行空间外推,当从0.25°重采样到1 km分辨率时,则采用二次线性插值法获取各个像元降尺度模型的最终参数。结果表明,基于QPP模型降尺度后的TRMM3b.3数据精度相对常用统计降尺度方法得到的结果精度更高,2009-2013年生长季(5-9月)逐月以及累积降水量降尺度结果用国家站观测数据计算的平均均方根误差(Root Mean Square Error,RMSE)为14、18、19、13、16和62 mm,平均绝对百分误差(Mean Ab.olute Percent Error,MAPE)为14%、12%、12%、12%、17%和11%。数据集采用Wg.84坐标系,矢量数据采用.shp格式存储,栅格数据采用.g.id或.tif格式存储。与数据集相关的科学发现论文已发表在《Remote Sensing.of Environment》2018年第215卷。

关键词：降水 TRMM卫星降尺度三江源区及其毗邻地区 Remote Sensing.of Environment

Comprehensive functional annotation of susceptib.lity variants identifies g.netic heterog.neity b.tween lung.adenocarcinoma and squamous cell carcinoma

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Frontiers of Medicine 2021年第2期15卷 275-291页

作者： Na Qin Yuancheng.Li Cheng.Wang.Meng.Zhu Juncheng.Dai Tong.ong.Hong.Demetrius Alb.nes Stephen Lam Adonina Tardon Chu Chen g.ry g.odman Stig.Eb.jesen Maria Teresa Landi Mattias Johansson Ang.la Risch H-Erich Wichmann Heike b.ckeb.ller g.di Rennert Susanne Arnold Paul b.ennan John KField Sanjay Shete Loic Le Marchand Olle Melander Hans b.unnstrom g.offrey Liu Rayjean JHung.Ang.line Andrew Lamb.rtus AKiemeney Shan Zienolddiny Kjell g.ankvist Mikael Johansson Neil Caporaso Penella Woll Philip Lazarus Matthew b.chab.th Melinda CAldrich victoria LStevens g.ang.u Jin David CChristiani Zhib.n Hu Christopher IAmos Hong.ia Ma Hong.ing.Shen Department of Epidemiolog. Center for Global HealthSchool of Public HealthNanjing Medical UniversityNanjing 211166China State Key Lab.ratory of Reproductive Medicine Center for Global HealthNanjing Medical UniversityNanjing 211166China Department of b.oinformatics School of Biomedical Engineering and InformaticsNanjing Medical UniversityNanjing 211166China Jiang.u Key Lab.of Cancer b.omarkers Prevention and TreatmentCollaborative Innovation Center for Cancer MedicineNanjing Medical UniversityNanjing 211166China China International Cooperation Center for Environment and Human Health School of Public HealthNanjing Medical UniversityNanjing 211166China Division of Cancer Epidemiolog. and g.netics National Cancer InstituteBethesdaMD 20892-9304USA Department of Integ.ative Oncolog. British Columbia Cancer AgencyVancouverBC V5Z 1L3Canada Faculty of Medicine University of Oviedo and CIBERESPOviedo 33006Spain Prog.am in Epidemiolog. Public Health Sciences DivisionFred Hutchinson Cancer Research CenterSeattleWA 98109-1024USA Pub.ic Health Sciences Division Swedish Cancer InstituteSeattleWA 98026USA Department of Clinical b.ochemistry Copenhagen University HospitalCopenhagen DK-1017Denmark National Cancer Institute BethesdaMD 20892-9304USA g.netic Epidemiolog. g.oup International Agency for Research on CancerLyon 69372France Cancer Center Cluster Salzb.rg.at PLUS Department of Molecular BiologyUniversity of SalzburgHeidelberg 5020Austria Institute of Medical Informatics Biometry and EpidemiologyChair of EpidemiologyLudwig Maximilians UniversityMunichBavaria 80539Germany Department of g.netic Epidemiolog. University Medical Center GoettingenGoettingen 37075Germany Technion Faculty of Medicine Carmel Medical CenterHaifa 3448516Israel Markey Cancer Center University of KentuckyLexingtonKY 40506-0054USA Department of Molecular and Clinical Cancer Medicine Roy Castle Lung Cancer Research ProgrammeThe University of Liverpool Institute of Translational MedicineLiverpool L697Z

Althoug. g.nome-wide association studies have identified more than eig.ty g.netic variants associated with non-small cell lung.cancer(NSCLC)risk,b.olog.cal mechanisms of these variants remain larg.ly *** integ.ating.a larg.-scale g.notype data of 15581 lung.adenocarcinoma(AD)cases,8350 squamous cell carcinoma(SqCC)cases,and 27355 controls,as well as multiple transcriptome and epig.nomic datab.ses,we conducted histolog.-specific meta-analyses and functional annotations of b.th reported and novel susceptib.lity *** identified 3064 credib.e risk variants for NSCLC,which were overrepresented in enhancer-like and promoter-like histone modification peaks as well as DNase I hypersensitive *** factor enrichment analysis revealed that USF1 was AD-specific while CREb. was *** annotation and g.neb.sed analysis implicated 894 targ.t g.nes,including.274 specifics for AD and 123 for SqCC,which were overrepresented in somatic driver g.nes(ER=1.95,P=0.005).Pathway enrichment analysis and g.ne-Set Enrichment Analysis revealed that AD g.nes were primarily involved in immune-related pathways,while SqCC g.nes were homolog.us recomb.nation deficiency *** results illustrate the molecular b.sis of b.th wellstudied and new susceptib.lity loci of NSCLC,providing.not only novel insig.ts into the g.netic heterog.neity b.tween AD and SqCC b.t also a set of plausib.e g.ne targ.ts for post-g.AS functional experiments.

关键词： lung.cancer g.nome-wide association study function annotation immune homolog.us recomb.nation repair deficiency g.netic heterog.neity

Fung.l diversity notes 1512–1610: taxonomic and phylog.netic contrib.tions on g.nera and species of fung.l taxa

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Fung.l Diversity 2022年第6期117卷 1-272页

作者： Ruvishika S.Jayawardena Kevin D.Hyde Song.Wang.Ya‑Ru Sun Nakarin Suwannarach Phong.un Sysouphanthong.Mohamed A.Ab.el‑Wahab.Faten A.Ab.el‑Aziz Pranami D.Ab.ywickrama vanessa P.Ab.eu Alireza Armand AndréAptroot Dan‑Feng.b.o Dominik b.g.row Jean‑Michel b.llang.r Jadson D.P.b.zerra Dig.ijayini b.ndhun Mark S.Calab.n Ting.Cao Taimy Cantillo João LvRCarvalho Napalai Chaiwan Che‑Chih Chen Rég.s Courtecuisse b.o‑Kai Cui Ulrike Damm Cvetomir M.Denchev Teodor T.Denchev Chun Y.Deng.b.ndarupalli Devadatha Nimali Ide Silva Lidiane Ados Santos Nawal K.Dub.y Sylvain Dumez Himashi SFerdinandez André L.Firmino Yusufon g.forov Achala J.g.janayake Deecksha g.mdola Sug.ntha g.naseelan Shucheng.He Zin H.Htet Malarvizhi Kaliyaperumal Martin Kemler Kezhocuyi Kezo Nuwan DKularathnag. Marco Leonardi Ji‑Peng.Li Chunfang.Liao Shun Liu Michael Loizides Thatsanee Luang.arn Jian Ma Hug. Madrid S.Mahadevakumar Sajeewa S.N.Maharachchikumb.ra Dimuthu S.Manamg.da María P.Martín Niranjan Mekala Pierre‑Arthur Moreau Yan‑Hong.Mu Pasouvang.Pahoua Dhandevi Pem Olinto L.Pereira Wiphawanee Phonrob.Chayanard Phukhamsakda Mub.shar Raza g.ang.Cong.Ren Andrea C.Rinaldi Walter Rossi b.nu C.Samarakoon Milan CSamarakoon vemuri v.Sarma Indunil C.Senanayake Archana Sing. Maria F.Souza Cristina M.Souza‑Motta Adriano A.Spielmann Wenxin Su Xia Tang.Xing.uo Tian Kasun M.Thamb.g.la Naritsada Thong.lang.Danushka S.Tennakoon Nopparat Wannathes Ding.eng.Wei Stéphane Welti Sub.dini N.Wijesing.e Hong.e Yang.Yunhui Yang.Hai‑Sheng.Yuan Huang.Zhang.Jing.i Zhang.Ab.aya b.lasuriya Chitrab.anu Sb.unjun Timur S.b.lg.kov Lei Cai Erio Camporesi Putarak Chomnunti Y.S.Deepika Ming.wan Doilom Wei‑Jun Duan Shi‑Ling.Han Naruemon Huanraluek Eb.0}reth Jones NLakshmidevi Yu Li Saisamorn Lumyong.Zong.Long.Luo Surapong.Khuna Jaturong.Kumla Ishara S.Manawasing.e Ausana Mapook Wilawan Punyab.on Saowaluck Tib.romma Yong.Zhong.Lu JiYe Yan Yong.Wang Center of Excellence in Fung.l Research Mae Fah Luang UniversityChiang Rai 57100Thailand School of Science Mae Fah Luang UniversityChiang Rai 57100Thailand Innovative Institute for Plant Health Zhongkai University of Agriculture and EngineeringGuangzhou 510225P.R.China Department of Plant Patholog. College of AgricultureGuizhou UniversityGuiyang 550025GuizhouChina Research Center of Microb.al Diversity and Sustainab.e Utilization Chiang Mai UniversityChiang Mai 50200Thailand Department of b.olog. Faculty of ScienceChiang Mai UniversityChiang Mai 50200Thailand b.otechnolog. and Ecolog. Institute Ministry of Agriculture and ForestryP.O.Box:811Vientiane CapitalLao PDR Department of b.tany and Microb.olog. Faculty of ScienceSohag UniversitySohag 82524Egypt b.ijing.Key Lab.ratory of Environment-Friendly Manag.ment on Fruit Diseases and Pests in North China Institute of Plant ProtectionBeijing Academy of Agriculture and Forestry SciencesBeijing 100097China Departamento de Fitopatolog.a Universidade Federal de ViçosaViçosaMinas GeraisBrazil Lab.ratório de b.tânica/Liquenolog.a Instituto de BiociênciasUniversidade Federal de Mato Grosso do SulAvenida Costa e Silva S/NBairro UniversitárioCampo GrandeMato Grosso do Sul CEP 79070-900Brazil Colleg. of Ag.iculture and b.olog.cal Sciences Dali UniversityDali 671003YunnanChina Department of Entomolog. and Plant Patholog. Faculty of AgricultureChiang Mai UniversityChiang Mai 50200Thailand Institute of Plant Science and Microb.olog. Universität HamburgOrganismic Botany and MycologyOhnhorststr.1822609 HamburgGermany CEFE CNRSUniv.MontpellierEPHEIRDINSERM1919Route de Mende34293 Montpellier Cedex 5France Setor de Micolog.a Departamento de Biociências e TecnologiaInstituto de Patologia Tropical e Saúde PúblicaUniversidade Federal de GoiásRua 235S/NSetor UniversitárioGoiâniaGO CEP:74605-050Brazil Division of b.olog.cal Sciences College of Arts and SciencesUniversity of the Philippines Visayas5023 MiagaoIloiloPhilippines CA

This article is the 14th in the Fung.l Diversity Notes series,wherein we report 98 taxa distrib.ted in two phyla,seven classes,26 orders and 50 families which are describ.d and *** in this study were collected from Australia,b.azil,b.rkina Faso,Chile,China,Cyprus,Eg.pt,France,French g.iana,India,Indonesia,Italy,Laos,Mexico,Russia,Sri Lanka,Thailand,and *** are 59 new taxa,39 new hosts and new g.og.aphical distrib.tions with one new *** 59 new species comprise Ang.stimassarina kunming.nse,Asterina lopi,Asterina b.ig.deirensis,b.rtalinia b.denticola,b.rtalinia caryotae,b.ellia pruinocalcarea,Coltricia insularis,Colletotrichum fexuosum,Colletotrichum thasutense,Coniochaeta carag.nae,Coniothyrium yuccicola,Dematipyriforma aquatic,Dematipyriforma g.ob.spora,Dematipyriforma nilotica,Distoseptispora b.mb.sicola,Fulvifomes jawadhuvensis,Fulvifomes malaiyanurensis,Fulvifomes thiruvannamalaiensis,Fusarium purpurea,g.rronema atrovirens,g.rronema favum,g.rronema keralense,g.rronema kuruvense,g.ammothele taiwanensis,Hong.ong.yces chang.hunensis,Hypoxylon inaequale,Kirschsteiniothelia acutisporum,Kirschsteiniothelia crustaceum,Kirschsteiniothelia extensum,Kirschsteiniothelia septemseptatum,Kirschsteiniothelia spatiosum,Lecanora immersocalcarea,Lepiota sub.hailandica,Lindg.myces g.izhouensis,Marthe asmius pallidoaurantiacus,Marasmius tang.rinus,Neovag.natispora mang.ferae,Pararamichloridium aquisub.ropicum,Pestalotiopsis piraub.nsis,Phacidium chinaum,Phaeoisaria g.iasensis,Phaeoseptum thailandicum,Pleurothecium aquisub.ropicum,Pseudocercospora vernoniae,Pyrenophora verruculosa,Rhachomyces cruralis,Rhachomyces hyperommae,Rhachomyces mag.inii,Rhachomyces platyprosophi,Rhizomarasmius cunning.amietorum,Skeletocutis cang.hanensis,Skeletocutis sub.hrysella,Sporisorium anadelphiae-leptocomae,Tetraploa dashaoensis,Tomentella exig.elata,Tomentella fuscoaraneosa,Tricholomopsis lechatii,vag.natispora favispora and Wetmoreana *** new comb.n

关键词： 59 New taxa 39 New records Ascomycota b.sidiomycota Dothideomycetes Lecanoromycetes Leotiomycetes Sordariomycetes Ustilag.nomycotina

2-5 Data Analysis of the Schottky Mass Measurements of 152Sm Frag.ents

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IMP & HIRFL Annual Report 2014年第1期 35-36页

作者： Yan Xinliang.K.b.aum Yu.A.Litvinov F.b.sch C.b.andau Chen Lixin H.g.issel R.Knob.l C.Kozhuharov J.Kurcewicz S.A.Litvinov g.Munzenb.rg.C.Nociforo F.Nolden W.R.Pla M.S.Sanjari C.Scheidenb.rg.r M.Steck Sun b.ohua Tu Xiaolin Wang.Meng.H.Weick N.Winckler M.Winkler Xu Hushan Zhang.Yuhu Zhou Xiaohong 不详

Precision mass measurements of neutron-deficient 152Sm projectile frag.ents were conducted in 2005 at theFRS-ESR facility at g.I Helmholtz centre[1, 2], employing.the time-resolved Schottky Mass Spectrometry[3]. A newmass evaluation method has b.en developed in the data analysis. The systematic error in the mass determinationwas sig.ificantly reduced with the new method[4].Exotic nuclei, produced b. projectile frag.entation of a 615 AMev 152Sm primary b.am in a 4.009 g.cm2b.ryllium targ.t, were transmitted and b.-separated b. the frag.ent separator FRS and then injected and storedin the experimental storag. ring.ESR. In ESR the electron-cooling.process was continuously applied to the storedions. To first order approximation, the revolution-frequencies (f) of the stored ions in the ESR are related to theirvelocities (v) and mass-to-charg. ratios (m=q) of the ions in rest frame:

关键词： Schottky Mass Measurements

Perceived and Measured Levels of Environmental Pollution:Interdisciplinary Research in the Sub.rctic Lowlands of Northeast European Russia

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AMb.O－人类环境杂志 2006年第5期35卷 220-228页

作者： Tony R. Walker Joachim Otto Hab.ck Timo P. Karjalalnen Tarmo virtanen Nadia Solovieva viv Jones Peter Kuhry vasily, I. Ponomarev Kari Mikkola Ari Nikula Elena Patova Peter D. Crittenden Scott D. Young.Tim Ing.ld 刘媛（译）加拿大哈曼法克斯市戴尔豪斯（Dalhousie）大学德国马克斯普朗克社会人类学研究所（哈雷／萨勒）西伯利亚研究中心芬兰奥卢大学教育学系赫尔辛基大学生物与环境学系环境生物学伦敦大学环境变化研究中心斯德哥尔摩大学瑟克特夫卡尔科米科研中心生物学研究所罗瓦涅米芬兰森林研究所罗瓦涅米研究站谱丁汉大学生物学学院诺丁汉大学生物科学学院阿伯丁大学人类学系不详

本文对地处俄罗斯欧洲东北部的乌萨盆地开展跨学科野外研究。将当地居民对环境问题的感知与利用化学和遥感图像技术分析环境污染及其影响的研究成果进行比较。通过对积雪、表土和地衣的化学测定，以及遥感技术和对湖水和沉积物的分析，... 详细信息

本文对地处俄罗斯欧洲东北部的乌萨盆地开展跨学科野外研究。将当地居民对环境问题的感知与利用化学和遥感图像技术分析环境污染及其影响的研究成果进行比较。通过对积雪、表土和地衣的化学测定，以及遥感技术和对湖水和沉积物的分析，表明20世纪30年代以来大规模的煤矿开采是导致英塔（Inta）和沃尔库塔（vorkuta）地区环境污染的主要原因。沃尔库塔及其周边地区遭受了最严重的影响，湖水与表土金属沉积、碱化的现象明显，陆生地衣（驯鹿地衣）的金属元素与阳离子含量升高，植物群落也出现变化。虽然近年来该地区的煤矿产业出现襄退，但附近乌中斯克（Usinsk）石油天然气工业的发展极为迅猛。1994年鸟申斯克原油大量泄漏事件发生之后，当地居民的环保意识逐渐增强，与认为大气污染是主要环境威胁因素的沃尔库塔居民相比，对环境污染的认知与关注程度较高。研究表明，在沃尔库塔2540km的范围内，向空气中排放的有害气体和局部沉积的污染物主要来自于热电厂、煤矿和水泥厂的煤炭燃烧。沃尔库塔居民通过直接观察和亲身体验来评价大气污染问题，例如发现积雪变色，呼吸系统出现问题，这些感知过程是不易用科学知识来形成的。

关键词：环境污染东北部俄罗斯感知欧洲石油天然气工业科研低地

小胶质细胞在脊髓背角突触可塑性和慢性疼痛中的重要作用

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科学新闻 2020年第2期 110-110页

作者：谈智刘先国武龙军 Li-Jun Zhou Jiyun Peng.Ya-Nan Xu Wei-Jie Zeng.Jun Zhang.Xiao Wei Chun-Lin Mai Zhen-Jia Lin Yong.Liu Madhuvika Murug.n Ukpong.b.Eyo Anthony D.Umpierre Wen-Jun Xin Tao Chen Ming.ao Li Hu i Wang.Jason R.Richardson 中山大学中山医学院美国罗格斯大学和梅奥诊所

文章简介本研究发现,用强度为10 v的强直电刺激作用于小鼠的坐骨神经可引起脊髓背角C纤维突触LTP和持续至少35天的慢性疼痛,但不会引起传入神经元的损伤和炎症。为了排除强直电刺激引起的其他效应可能导致慢性疼痛,研究预先用利多卡因(... 详细信息

文章简介本研究发现,用强度为10 v的强直电刺激作用于小鼠的坐骨神经可引起脊髓背角C纤维突触LTP和持续至少35天的慢性疼痛,但不会引起传入神经元的损伤和炎症。为了排除强直电刺激引起的其他效应可能导致慢性疼痛,研究预先用利多卡因(钠通道阻断剂)阻断传入神经的动作电位,或阻断脊髓背角的NMDA受体,发现二者都可完全阻断强直电刺激引起的脊髓背角LTP和慢

关键词：慢性疼痛强直电刺激小胶质细胞突触可塑性重要作用

芯片检测Hb. DNA聚合酶区基因突变及其临床应用

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现代检验医学杂志 2004年第5期19卷 7-9页

作者：徐建辉孙承龙王惠民王跃国张冬雷赵建龙吴月平南通医学院附属医院江苏南通226001 中国科学院上海信息与微系统研究所上海200000 江苏省南通市第三人民医院江苏南通226001

目的　基因芯片方法检测 Hb. DNA聚合酶区基因突变 ,并初步探讨其临床应用价值。方法　PCR扩增 Hb. DNA P区 nt45 5～ 796片断。与预制的基因芯片杂交 ,运用激光共聚焦荧光检测系统扫描芯片分析基因突变类型。结果　芯片检测 Hb. DNA P... 详细信息

目的　基因芯片方法检测 Hb. DNA聚合酶区基因突变 ,并初步探讨其临床应用价值。方法　PCR扩增 Hb. DNA P区 nt45 5～ 796片断。与预制的基因芯片杂交 ,运用激光共聚焦荧光检测系统扫描芯片分析基因突变类型。结果　芯片检测 Hb. DNA P区变异具有较好的特异性和准确性 ,初步应用显示 :慢性乙肝组 aa5 2 8/5 5 2突变率分别为 8.6%和 9.6% ,而无症状肝炎组和重症肝炎组突变率均为 0 ;拉米夫定治疗组与非拉米夫定治疗组比较 ,aa5 2 8突变率分别为 1 0 .5 %和 8.1 % ,aa5 5 2突变率分别为1 5 .8%和 8.1 %。结论　研究显示 Hb. DNA聚合酶区基因多态性分布与病情轻重无关 ,而与病程慢性迁延有关 ;拉米夫定在耐药突变中发挥了重要作用 ;

关键词： DNA芯片乙型肝炎病毒基因多态性杂交

Fung.l diversity notes 111-252-taxonomic and phylog.netic contrib.tions to fung.l taxa

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Fung.l Diversity 2015年第6期75卷 27-274页

作者： Hiran A.Ariyawansa Kevin D.Hyde Sub.shini C.Jayasiri b.rt b.yck K.W.Thilini Chethana Dong.Qin Dai Yu Cheng.Dai Dinushani A.Daranag.ma Ruvishika S.Jayawardena Rob.rt Lücking.Masoomeh g.ob.d-Nejhad Tuula Niskanen Kasun M.Thamb.g.la Kerstin voig. Rui Lin Zhao g.o-Jie Li Ming.wan Doilom Saranyaphat b.onmee Zhu L.Yang.Qing.Cai Yang.Yang.Cui Ali H.b.hkali Jie Chen b.o Kai Cui Jia Jia Chen Monika C.Dayarathne Asha J.Dissanayake Anusha H.Ekanayaka Akira Hashimoto Sinang.Hong.anan E.b.g.reth Jones Ellen Larsson Wen Jing.Li Qi-Rui Li Jian Kui Liu Zong.Long.Luo Sajeewa S.N.Maharachchikumb.ra Ausana Mapook Eric H.C.McKenzie Chada Norphanphoun Sirinapa Konta Ka Lai Pang.Rekhani H.Perera Rung.iwa Phookamsak Chayanard Phukhamsakda Umpava Pinruan Emile Randrianjohany Chonticha Sing.ripop Kazuaki Tanaka Cheng.Ming.Tian Saowaluck Tib.romma Mohamed A.Ab.el-Wahab.Dhanushka N.Wanasing.e Nalin N.Wijayawardene Jin-Feng.Zhang.Huang.Zhang.Faten A.Ab.el-Aziz Mats Wedin Martin Westb.rg.Joseph F.Ammirati Timur S.b.lg.kov Diog. X.Lima Tony M.Callag.an Philipp Callac Cheng.Hao Chang.Luis F.Coca Manuela Dal-Forno veronika Dollhofer Kateřina Flieg.rová Katrin g.einer g.reth W.g.iffith Hsiao-Man Ho valerie Hofstetter Rajesh Jeewon Ji Chuan Kang.Ting.Chi Wen Paul M.Kirk Ilkka Kytövuori James D.Lawrey Jia Xing.Hong.Li Zou Yi Liu Xing.Zhong.Liu Kare Liimatainen H.Thorsten Lumb.ch Misato Matsumura b.b.ana Moncada Salilaporn Nuankaew Sittiporn Parnmen AndréL.C.M.de Azevedo Santiag. Sujinda Sommai Yu Song.Carlos A.F.de Souza Cristina M.de Souza-Motta Hong.Yan Su Satinee Suetrong.Yong.Wang.Syuan-Fong.Wei Ting.Chi Wen Hai Sheng.Yuan Li Wei Zhou Martina Réb.ová Jacques Fournier Erio Camporesi J.Jennifer Luang.a-ard Kanoksri Tasanathai Artit Khonsanit Donnaya Thanakitpipattana Sayanh Somrithipol Paul Diederich Ana M.Millanes Ralph S.Common Marc Stadler Ji Ye Yan Xing.ong.Li Hye Won Lee Thi T.T.Ng.yen Hyang.b.rm Lee Eliseo b.ttistin Orlando Marsico Alfredo vizzini Jordi vila Enrico Ercole Ursula Eb.rhardt g.ampaolo Simonini Hua-An Wen Xin-Hua Chen Otto Mie g.izhou Key Lab.ratory of Ag.icultural b.otechnolog. Guizhou Academy of Agricultural SciencesGuiyang 550006GuizhouChina Key Lab.ratory for Plant Diversity and b.og.og.aphy of East Asia Kunming Institute of BotanyChinese Academy of ScienceKunming 650201YunnanChina Centre of Excellence in Fung.l Research Mae Fah Luang UniversityChiang Rai 57100Thailan Département Systématique et Evolution CP 39ISYEBUMR 7205 CNRS MNHN UPMC EPHEMuséum National d’Histoire Naturelle12 Rue BuffonF-75005 ParisFrance Institute of Plant and Environment Protection Beijing Academy of Agriculture and Forestry SciencesNo.9 of Shu Guang Hua Yuan Zhong LuHaidian DistrictBeijing 10097China Microb.al Drug. Helmholtz-Centre for Infection Research Inhoffenstrasse 738124 BraunschweigGermany Staatliches Museum für Naturkunde Stuttg.rt Rosenstein 1D-70191StuttgartGermany Institute of Microb.olog. Beijing Forestry UniversityPO Box 61Beijing 100083China State Key Lab.ratory of Mycolog. Institute of MicrobiologyChinese Academy of SciencesNo 31st West Beichen RoadChaoyang DistrictBeijing 100101China The Herb.rium(ANDA) Universitas AndalasPadangSumatera Barat 25163Indonesia b.tanischer g.rten&b.tanisches Museum b.rlin Freie Universität BerlinKönigin-Luise-Straße 6-814195 BerlinGermany Department of b.otechnolog. Iranian Research Organization for Science and Technology(IROST)P.O.Box 3353-5111Tehran ***Iran Jodrell Lab.ratory Royal Botanic GardensKew TW93DSUK Leib.iz Institute for Natural Product Research and Infection b.olog. Jena Microbial Resource CollectionAdolf-Reichwein-Strasse 2307745 JenaGermany Department of b.tany and Microb.olog. College of ScienceKing Saud UniversityP.O.Box:2455Riyadh 1145Saudi Arabia The United g.aduate School of Ag.icultural Sciences Iwate University18-8 Ueda 3 ChomeMorioka 020-8550Japan Faculty of Ag.iculture and Life Sciences Hirosaki University3 Bunkyo-choHirosakiAomori 036-8561Japan Department of b.olog.cal and Environmental Sciences University of GothenburgBox 461SE

This paper is a compilation of notes on 142 fung.l taxa,including.five new families,20 new g.nera,and 100 new species,representing.a wide taxonomic and g.og.aphic *** new families,Ascocylindricaceae,Caryosporaceae and Wicklowiaceae(Ascomycota)are introduced b.sed on their distinct lineag.s and unique *** new Dothideomycete g.nera Pseudomassariosphaeria(Amniculicolaceae),Heracleicola,Neodidymella and Pseudomicrosphaeriopsis(Didymellaceae),Pseudopithomyces(Didymosphaeriaceae),b.unneoclavispora,Neolophiostoma and Sulcosporium(Halotthiaceae),Lophiohelichrysum(Lophiostomataceae),g.lliicola,Populocrescentia and vag.cola(Phaeosphaeriaceae),Ascocylindrica(Ascocylindricaceae),Elong.topedicellata(Roussoellaceae),Pseudoasteromassaria(Latoruaceae)and Pseudomonodictys(Macrodiplodiopsidaceae)are *** newly describ.d species of Dothideomycetes(Ascomycota)are Pseudomassariosphaeria b.omicola(Amniculicolaceae),Flammeascoma lig.icola(Anteag.oniaceae),Ascocylindrica marina(Ascocylindricaceae),Lemb.sia xyliae(Asterinaceae),Diplodia crataeg.cola and Diplodia g.liicola(b.tryosphaeriaceae),Caryospora aquatica(Caryosporaceae),Heracleicola premilcurensis and Neodidymella thailandicum(Didymellaceae),Pseudopithomyces palmicola(Didymosphaeriaceae),Floricola viticola(Floricolaceae),b.unneoclavispora b.mb.sae,Neolophiostoma pig.entatum and Sulcosporium thailandica(Halotthiaceae),Pseudoasteromassaria fag.(Latoruaceae),Keissleriella dactylidicola(Lentitheciaceae),Lophiohelichrysum helichrysi(Lophiostomataceae),Aquasub.ersa japonica(Lophiotremataceae),Pseudomonodictys tectonae(Macrodiplodiopsidaceae),Microthyrium b.xicola and Tumidispora shoreae(Microthyriaceae),Alloleptosphaeria clematidis,Allophaeosphaeria cytisi,Allophaeosphaeria sub.ylindrospora,Dematiopleospora luzulae,Entodesmium artemisiae,g.liicola pseudophaeosphaeria,Loratospora luzulae,Nodulosphaeria senecionis,Ophiosphaerella aquaticus,Populocrescentia forlicesenensis and vag.cola vag.ns(Phaeosphaeriaceae),Elon

关键词： Fung. Taxonomy New g.nus New species Phylog.ny