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Influence of norcantharidin on proliferation,proliferation-related gene proteins prolifera-ting cell nuclear antigen and Ki-67 of human gallbladder carcinoma GBC-SD cells

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Hepatobiliary & Pancreatic Diseases International 2004年第4期3卷 603-607页

作者： Yue-Zu Fan, Jin-Ye fu, ze-Ming zhao and Cun-Qiu Chen Shanghai, China Department of Surgery, Tongji Hospital of Tongji U- mversity, Shanghai 200065, China Department of Surgery, Pudong People’ s Hospital, Shanghai 201200 , Chi- na Department of Surgery Tongji Hospital of Tongji University 389 Xincun Road Shanghai 200065 China. fanyuezu_shtj@***

BACKGROUND: Gallbladder carcinoma is a highly lethal and aggressive disease with early metastasis, strong invasion and poor prognosis. Most patients with this disease are at the advanced and un-resectable stage and should be consi- dered for palliative treatment such as chemotherapy and ra- diotherapy. Unfortunately, reports of chemotherapy and radiotherapy for gallbladder carcinoma are disappointing. We investigated the influence of norcantharidin (NCTD) on proliferation, proliferation-related gene proteins PCNA and Ki-67 of human gallbladder carcinoma GBC-SD cells in vitro. METHODS: GBC-SD cell lines of human gallbladder carci- noma were cultured by the cell culture technique. The ex- periment was divided into NCTD group and control group. The tetrazolium-based colorimetric assay was used to evaluate cell growth. The streptavidin-biotin complex method was used to determine the expressions of prolifera- tion-related gene proteins PCNA and Ki-67 of human gall- bladder carcinoma GBC-SD cells. RESULTS: NCTD inhibited the growth and proliferation of GBC-SD cells from 10 mg/L or after 6 hours in a dose- and time-dependent manner, with the IC50 value of 56.18 μg/ ml at 48 hours. After treatment with NCTD, the expression of PCNA (0.932 ±0.031 vs. 0.318 ±0.023, P<0.001) and Ki-67 (0.964 ±0.092 vs. 0.297 ±0.018, P<0.001) proteins were decreased significantly. CONCLUSION: NCTD inhibits the proliferation of human gallbladder carcinoma GBC-SD cells in vitro and the expres- sion of their proliferation-related gene proteins PCNA and Ki-67.

关键词： gallbladder neoplasm norcantharidin cell culture immunohistochemistry cell proliferation proliferating cell nuclear antigen, Ki-67

MXene Hybridized Polymer with Enhanced Electromagnetic Energy Harvest for Sensitized Microwave Actuation and Self-Powered Motion Sensing

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NANO-MICRO LETTERS 2025年第1期17卷 1-15页

作者： Wang, Yu-ze Wang, Yu-Chang Liu, Ting-Ting zhao, Quan-Liang Li, Chen-Sha Cao, Mao-Sheng Beijing Inst Technol Sch Mat Sci & Engn Beijing 100081 Peoples R China Peking Univ Sch Mat Sci & Engn Beijing 100871 Peoples R China North China Univ Technol Sch Mech & Mat Engn Beijing 100144 Peoples R China Heilongjiang Univ Minist Educ Peoples Republ China Key Lab Funct Inorgan Mat Chem Harbin 150080 Peoples R China

Polymeric microwave actuators combining tissue-like softness with programmable microwave-responsive deformation hold great promise for mobile intelligent devices and bionic soft robots. However, their application is challenged by restricted electromagnetic sensitivity and intricate sensing coupling. In this study, a sensitized polymeric microwave actuator is fabricated by hybridizing a liquid crystal polymer with Ti3C2Tx (MXene). Compared to the initial counterpart, the hybrid polymer exhibits unique space-charge polarization and interfacial polarization, resulting in significant improvements of 230% in the dielectric loss factor and 830% in the apparent efficiency of electromagnetic energy harvest. The sensitized microwave actuation demonstrates as the shortened response time of nearly 10 s, which is merely 13% of that for the initial shape memory polymer. Moreover, the ultra-low content of MXene (up to 0.15 wt%) benefits for maintaining the actuation potential of the hybrid polymer. An innovative self-powered sensing prototype that combines driving and piezoelectric polymers is developed, which generates real-time electric potential feedback (open-circuit potential of similar to 3 mV) during actuation. The polarization-dominant energy conversion mechanism observed in the MXene-polymer hybrid structure furnishes a new approach for developing efficient electromagnetic dissipative structures and shows potential for advancing polymeric electromagnetic intelligent devices.

关键词： Microwave absorption Electromagnetic response Energy harvest Self-sensing Soft actuator

Human neural stem cell-derived extracellular vesicles protect against ischemic stroke by activating the PI3K/AKT/mTOR pathway

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Neural Regeneration Research 2025年第11期20卷 3245-3258页

作者： Jiayi Wang Mengke zhao Dong fu Meina Wang Chao Han Zhongyue Lv Liang Wang Jing Liu Stem Cell Clinical Research Center The First Affiliated Hospital of Dalian Medical UniversityDalianLiaoning ProvinceChina Dalian Innovation Institute of Stem Cell and Precision Medicine DalianLiaoning ProvinceChina Institute(College)of Integrative Medicine Dalian Medical UniversityDalianLiaoning ProvinceChina

Human neural stem cell-derived extracellular vesicles exhibit analogous functions to their parental cells,and can thus be used as substitutes for stem cells in stem cell therapy,thereby mitigating the risks of stem cell therapy and advancing the frontiers of stem cell-derived *** lays a foundation for the development of potentially potent new treatment modalities for ischemic ***,the precise mechanisms underlying the efficacy and safety of human neural stem cell-derived extracellular vesicles remain unclear,presenting challenges for clinical *** promote the translation of therapy based on human neural stem cell-derived extracellular vesicles from the bench to the bedside,we conducted a comprehensive preclinical study to evaluate the efficacy and safety of human neural stem cell-derived extracellular vesicles in the treatment of ischemic *** found that administration of human neural stem cell-derived extracellular vesicles to an ischemic stroke rat model reduced the volume of cerebral infarction and promoted functional recovery by alleviating neuronal *** human neural stem cell-derived extracellular vesicles reduced neuronal apoptosis by enhancing phosphorylation of phosphoinositide 3-kinase,mammalian target of rapamycin,and protein kinase B,and these effects were reversed by treatment with a phosphoinositide 3-kinase *** findings suggest that human neural stem cell-derived extracellular vesicles play a neuroprotective role in ischemic stroke through activation of phosphoinositide 3-kinase/protein kinase B/mammalian target of rapamycin signaling ***,we showed that human neural stem cell-derived extracellular vesicles have a good in vivo safety ***,human neural stem cell-derived extracellular vesicles are a promising potential agent for the treatment of ischemic stroke.

关键词： behavior exosome extracellular vesicles ischemic stroke mammalian target of rapamycin(mTOR) middle cerebral artery occlusion neural stem cells neuronal apoptosis phosphoinositide 3-kinase(PI3K) protein kinase B(AKT)

As-cast structure and tensile properties of AZ80 magnesium alloy DC cast with low-voltage pulsed magnetic field

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Transactions of Nonferrous Metals Society of China 2015年第7期25卷 2165-2171页

作者：罗天骄冀焕明崔杰赵福泽冯小辉李应举杨院生中国科学院金属研究所沈阳110016

The effects of a low-voltage pulsed magnetic field on the solidified structure and mechanical properties of DC casting AZ80 magnesium alloy were *** results showed that the solidified structure of the DC casting AZ80 magnesium alloy was refined obviously by the low-voltage pulsed magnetic field and significant grain refinement in the DC casting ingot of AZ80 magnesium alloy was ***,the morphology of the dentritic in the DC casting ingot was transformed from coarse dentritic to fine rosette with the application of low-voltage pulsed magnetic *** ability of deformation of the ingot was enhanced and especially the plasticity of the ingot center after upsetting was improved greatly by more than 80%after deformation.

关键词： AZ80 magnesium alloy DC casting pulsed magnetic field grain refinement

Single-cell omics: experimental workflow, data analyses and applications

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Science China Life Sciences 2025年第1期68卷 5-102页

作者： Fengying Sun Haoyan Li Dongqing Sun Shaliu fu Lei Gu Xin Shao Qinqin Wang Xin Dong Bin Duan Feiyang Xing Jun Wu Minmin Xiao Fangqing zhao Jing-Dong J.Han Qi Liu Xiaohui Fan Chen Li Chenfei Wang Tieliu Shi Department of Clinical Laboratory the Affiliated Wuhu Hospital of East China Normal University (The Second People's Hospital of Wuhu City) Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang University Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration (Tongji University) Ministry of EducationOrthopaedic DepartmentTongji HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Frontier Science Center for Stem Cells School of Life Sciences and TechnologyTongji University Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine Shanghai East HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Research Institute of Intelligent Computing Zhejiang Lab Shanghai Research Institute for Intelligent Autonomous Systems Center for Single-cell Omics School of Public HealthShanghai Jiao Tong University School of Medicine National Key Laboratory of Chinese Medicine Modernization Innovation Center of Yangtze River DeltaZhejiang University Center for Bioinformatics and Computational Biology Shanghai Key Laboratory of Regulatory Biologythe Institute of Biomedical Sciences and School of Life SciencesEast China Normal University Beijing Institutes of Life Science Chinese Academy of Sciences Peking-Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary StudiesCenter for Quantitative Biology (CQB)Peking University Zhejiang Key Laboratory of Precision Diagnosis and Therapy for Major Gynecological Diseases Women's HospitalZhejiang University School of Medicine Key Laboratory of Advanced Theory and Application in Statistics and Data Science-MOE School of StatisticsEast China Normal University

Cells are the fundamental units of biological systems and exhibit unique development trajectories and molecular features. Our exploration of how the genomes orchestrate the formation and maintenance of each cell, and control the cellular phenotypes of various organismsis, is both captivating and intricate. Since the inception of the first single-cell RNA technology, technologies related to single-cell sequencing have experienced rapid advancements in recent years. These technologies have expanded horizontally to include single-cell genome, epigenome,proteome, and metabolome, while vertically, they have progressed to integrate multiple omics data and incorporate additional information such as spatial sc RNA-seq and CRISPR screening. Single-cell omics represent a groundbreaking advancement in the biomedical field,offering profound insights into the understanding of complex diseases, including cancers. Here, we comprehensively summarize recent advances in single-cell omics technologies, with a specific focus on the methodology section. This overview aims to guide researchers in selecting appropriate methods for single-cell sequencing and related data analysis.

关键词： single-cell sequencing genome epigenome proteomics metabolomics multimodal spatial transcriptomics CRISPR screening

Lipomatous ependymoma with ZFTA:RELA fusion-positive:A case report

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World Journal of Clinical Cases 2025年第1期13卷 48-55页

作者： Xiao-Yu zhao Juan-Han Yu Yi-Hua Wang Yi-Xin Liu Lu Xu Lin fu Ning Yi China Medical University The First Hospital of China Medical UniversityShenyang 110001Liaoning ProvinceChina Department of Pathology The First Hospital of China Medical UniversityShenyang 110001Liaoning ProvinceChina Anshan Cancer Hospital Anshan 114000Liaoning ProvinceChina

BACKGROUND Ependymoma with lipomatous differentiation is a rare type of *** ZFTA fusion-positive supratentorial ependymoma is a novel tumor type in the 2021 World Health Organization classification of central nervous system *** fusion-positive lipomatous ependymoma has not been reported to *** SUMMARY We reported a case of a 15-year-old Chinese male who had a sudden convulsion lasting approximately six *** resonance imaging showed a round cystic shadow of approximately 1.9 cm×1.5 cm×1.9 cm under the right parieto-occipital *** examination showed characteristic perivascular pseudorosettes and adipose differentiation in the ***-chemical staining showed that the tumor cells were negative for cytokeratin,NeuN,Syn and p53,but positive for GFAP,vimentin and S-100 ***-ficant punctate intracytoplasmic EMA immunoreactivity was *** level of Ki-67 was about 5%.Genetic analysis revealed ZFTA:RELA fusion.A cranio-tomy with total excision of the tumor was *** follow-up time was 36 months,no evidence of disease recurrence was found in magnetic resonance *** Based on these findings,the patient was diagnosed as a ependymoma with ZFTA fusion and lipomatous *** case report provides information on the microscopic morphological features of ependymoma with ZFTA fusion and lipomatous differentiation,which can help pathologists to make a definitive diagnosis of this tumor.

关键词： Ependymoma Lipomatous Molecular classification Supratentorial ZFTA:RELA fusion Case report

Solar evaporation for simultaneous oil-water separation and electricity generation with Janus wood-based absorbers

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Frontiers of Environmental Science & Engineering 2024年第2期18卷 13-23页

作者： Yue Yang ze fu Qi Zhang Jiangsu Key Laboratory of Chemical Pollution Control and Resources Reuse School of Environmental and Biological EngineeringNanjing University of Science&TechnologyNanjing 210094China Key Laboratory of Industrial Ecology and Environmental Engineering(Ministry of Education) School of Environmental Science and TechnologyDalian University of TechnologyDalian 116024China National Laboratory of Solid State Microstructures Collaborative Innovation Center of Advanced MicrostructuresCollege of Engineering and Applied SciencesKey Laboratory of Intelligent Optical Sensing and Manipulation(Ministry of Education)Jiangsu Key Laboratory of Artificial Functional MaterialsNanjing UniversityNanjing 210093China

Oily wastewater from ocean oil spills endangers marine ecosystems and human health. Therefore, developing an effective and sustainable solution for separating oil-water mixtures is urgent. Interfacial solar photothermal evaporation is a promising approach for the complete separation of two-phase mixtures using only solar energy. Herein, we report a carbonized wood-based absorber with Janus structure of comprising a hydrophobic top-layer and an oleophobic bottom-layer for simultaneous solar-driven oil-water separation and electricity generation. Under sunlight irradiation, the rapid evaporation of seawater will induce a separation of oil-water mixtures, and cause a high salt concentration region underlying the interface, while the bottom “bulk water” maintains in a low salt concentration, thus forming a salinity gradient. Electricity can be generated by salinity gradient power. Therefore, oil-water separation efficiency of > 99% and derived extra electricity power of ~0.1 W/m2 is achieved under solar radiation, demonstrating the feasibility of oil-water separation and electricity production synchronously directly using solar energy. This work provides a green and cost-effective path for the separation of oil-water mixtures.

关键词： Oily wastewater Carbonized wood Salinity gradient Electricity generation Solar irradiation

Comparison Between χ~2 and Bayesian Statistics with Considering the Redshift Dependence of Stretch and Color from JLA Data

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Communications in Theoretical Physics 2019年第9期71卷 1097-1108页

作者： ze zhao School of Physical Sciences University of Chinese Academy of Sciences CAS Key Laboratory of Theoretical Physics Institute of Theoretical PhysicsChinese Academy of Sciences Kavli Institute for Theoretical Physics China Chinese Academy of Sciences

In this work, we compare the impacts given by χ^2 statistics and Bayesian statistics. Bayesian statistics is a new statistical method proposed by [C. Ma, P. S. Corasaniti, and B. A. Bassett, arXiv:1603.08519[***](2016)]recently, which gives a fully account for the standard-candle parameter dependence of the data covariance matrix. For this two statistical methods, we explore the possible redshift-dependence of stretch-luminosity parameter α and colorluminosity parameter β by using redshift tomography. By constraining the ΛCDM model, we check the consistency of cosmology-fit results given by the SN sample of each redshift bin. We also adopt the linear parametrization to explore the possible evolution of α and β and the deceleration parameter q(z) for CPL, JBP, BA and Wang models. We find that:(i) Using the full JLA data, at high redshift α has a trend of decreasing at more than 1.5σ confidence level(CL),and β has a significant trend of decreasing at more than 19σ CL.(ii) Compared with χ^2 statistics(constant α, β) and Bayesian statistics(constant α, β), Bayesian statistics(linear α and β) yields a larger best-fit value of fractional matter density ?m0 from JLA+CMB+GC data, which is much closer to slightly deviates from the best-fit result given by other cosmological observations.(iii) The figure of merit(FoM) given by JLA+CMB+GC data from Bayesian statistics is also larger than the FoM from χ^2 statistics, which indicates that former statistics has a better accuracy.(iv) q(z) given by both statistical methods favor an eternal cosmic acceleration at 1σ CL.

关键词： cosmology dark energy observations cosmological parameters supernova

Diagnosing holographic type dark energy models with the Statefinder hierarchy, composite null diagnostic and w-w′pair

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Science China(Physics,Mechanics & Astronomy) 2018年第3期61卷 65-76页

作者： ze zhao Shuang Wang CAS Key Laboratory of Theoretical Physics Institute of Theoretical Physics Chinese Academy of Sciences School of Physical Science University of Chinese Academy of Sciences School of Astronomy and Space Science Sun Yat-Sen University

The main purpose of this work is to distinguish various holographic type dark energy （DE） models, including the AHDE, HDE, NADE, and RDE model, by using various diagnostic tools. The first diagnostic tool is the Statefinder hierarchy, in which the evolution of Statefinder hierarchy parmeter S3（1）（z） and S4（1）（z） are studied. The second is composite null diagnostic （CND）, in which the trajectories of {S（1）4, ∈} and {S4（1）, e} are investigated, where ∈ is the fractional growth parameter. The last is w-w′ analysis, where w is the equation of state for DE and the prime denotes derivative with respect to lna. In the analysis we consider two cases： varying current fractional DE density Ωde0 and varying DE model parameter C. We find that：（1） both the Statefinder S4（1） can lead to larger hierarchy and the CND have qualitative impact on AHDE, but only have quantitative impact on HDE. （2） S4（1） differences than S 4（1）, while the CND pair has a stronger ability to distinguish different models than the Statefinder hierarchy. （3） For the case of varying C, the {w, w′} pair has qualitative impact on AHDE; for the case of varying Ωde0, the {w, w′} pair only has quantitative impact; these results are different from the cases of HDE, RDE, and NADE, in which the {w, w′} pair only has quantitative impact on these models. In conclusion, compared with HDE, RDE, and NADE, the AHDE model can be easily distinguished by using these diagnostic tools.

关键词： dark energy cosmology cosmological constant