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Constructing Competitive Reverse Transcription Polymerize Chain Reaction Inter-Reference of PC mRNA by Intron Approach

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Agricultural Sciences in China 2004年第7期3卷 548-552页

作者： XUChuang XIACheng LIUGuo-wen WANGZhe JIANGYu-fu ZHANGNai-shen~ fuShi-xin FacultyofMilitaryVeterinaryMedicine QuartermasterUniversityofChinesePeople＇sLiberationArmyChangchun130062P.R.China

Inter-reference of competitive reverse transcription polymerase chain reaction (RT-PCR)was constructed by intron method to detect the change of PC mRNA level in the pathway ofcarbohydrate metabolism. The experiment based on the principle that 81bp intron sequencewas deleted in PC mRNA compared with PC DNA sequence. The 466bp competitive DNA templaterecombinant plasmid of PC mRNA was successfully built by a pair of primer and was clonedonce, PC DNA and PC mRNA could be inter-referred each other. The intron approach used inthe experiment has broken through the traditional method of constructing competitivetemplate.

关键词： PC Competitive template Intron Quantitative RT-PCR Clone

THE PHOTOACTIVITY OF SUBSTITUTED PHTHALOCYANINES TO PRODUCE ~1O_2

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Chinese Chemical Letters 1994年第2期5卷 115-116页

作者： Xian fu ZHANG and Tao shen(Institute of Photographic Chemistry, Academia Sinica, Beijing, 100101)

The guantum yields of 1O2 generated by photosensitization of some substituted metal phthalocyanlnes(Φ▲)were measured by using DPBF(diphenylisobenzofuran)as chemical trap of 1O2 in oxygen saturated DMSO solution. Both strong electron-withdrawing(-NO2)or strong electron-donating(-NH2)group decrease the ability to produce 1O2,whereas halogenation(Cl, Br, I)changes this ability only slightly.

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Blood pressure,baroreflex sensitivity,and end organ damage in hybrid offspring of spontaneously hypertensive rats and Spraque-Dawlev rats

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Acta Pharmacologica Sinica 2005年第9期26卷 1049-1056页

作者： He-hui XIE~2 fu-ming shen~2 Chao-yu MIAO Ding-feng SU~3 Department of Pharmacology,Second Military Medical University,Shanghai 200433,China Department of Pharmacology Second Military Medical University Shanghai China

Aim:To investigate the blood pressure(BP),baroreflex sensitivity(BRS),andorgan damage in hybrids of spontaneously hypertensive rats and ***:Spontaneously hypertensive rats and Sprague-Dawley rats werecrossbred,and the F1hybrids were inbred randomly to produce an *** the age of 52 weeks,the F1and F2hybrids were tested to determine BP and BRSin a conscious *** examinations were carried out after BPrecording and BRS ***:BP and BRS were not different in F1and *** was inversely related to systolic BP(SBP)in male,female,or wholepopulations of ***,BRS values were one-third determined bySBP level(the determinant coefficient was 0.326).The indexes for left ventricularhypertrophy,aortic hypertrophy,and renal damage were all positively related toBP,and negatively related to *** multiple-regression analysis,left ventricularand aortic hypertrophy and glomerulosclerosis score were all most significantlyassociated with lower BRS and higher systolic *** contribution of BRS to leftventricular and aortic hypertrophy and glomerulosclerosis was greater than thatof ***:The present work with hybrid rats demonstrated quantita-tively that the BRS value was one-third determined by SBP *** BP leveland BRS value contributed greatly to the hypertensive organ ***,the contribution of BRS to the hypertensive organ damage was greater than thatof BP level in these rats.

关键词： baroreflex blood pressure end organ damage hypertension genetic hybridization

Inflammasome-independent NALP3 contributes to high-salt induced endothelial dysfunction

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中国药理学与毒理学杂志 2019年第10期 796-797页

作者： fu Hui CHEN Ji-kuai LU Wen-jie JIANG Yu-jie WANG Yuan-yuan LI Dong-jie shen fu-ming Department of Pharmacy Shanghai Tenth People′ s Hospital Tongji University Department of Health Toxicology Faculty of Naval Medicine Second Military Medical University

OBJECTIVE Na+is an important nutrient and its intake, mainly from salt（Na Cl）, is essential for normal physiological function. However, high salt intake may lead to vascular injury, independent of a rise in blood pressure（BP）. Canonical NALP3 inflammasome activation is a caspase-1 medicated process, resulting in the secretion of IL-18 and IL-1β which lead to endothelial dysfunction. However, some researches uncovered a direct and inflammasome-independent role of NALP3 in renal injury. Thus, this study was designed to investigate the possible mechanisms of NALP3 in high salt induced endothelial dysfunction. METHODS and RESULTS Changes in endothelial function were measured by investigating mice(C57 BL/6 J, NALP3;and wild-type, WT)fed with normal salt diet（NSD） or high-salt diet（HSD） for12 weeks, and thoracic aortic rings from C57 BL/6 J mice cultured in high-salt medium. Changes of tube formation ability, intracellular reactive oxygen species（ROS）, and NALP3 inflammasome expression were detected using mouse aortic endothelial cells（MAECs） cultured in highsalt medium. Consumption of HSD for 12 weeks did not affect BP or body weight in C57 BL/6 J mice. Endotheliumdependent relaxation（EDR） decreased significantly in C57 BL/6 J mice fed with HSD for 12 weeks, and in isolated thoracic aortic rings cultured in high-salt medium for 24 *** from the aortic ring assay also revealed that the angiogenic function of thoracic aortas was impaired by either consumption of HSD or exposure to high-salt medium. NALP3;mice fed with HSD showed a relatively mild decrease in EDR function when compared with WT mice. Tube length of thoracic aortic rings from NALP3;mice was longer than those from WT mice after receiving high-salt treatment. Inhibiting NALP3 with a NALP3 antagonist, smal interfering（si） RNA experiments using si-RNALP3,and decomposing ROS significantly improved tube formation ability in MAECs under high salt medium. NALP3 expression was increased in MAECs cultured with hig

关键词： high salt intake endothelial-dependent relaxation angiogenic function NALP3 reactive oxygen species

Sodium Abundances in Very Metal-poor Stars

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Research in Astronomy and Astrophysics 2023年第7期23卷 229-246页

作者： Yu-fu shen S.A.Alexeeva Gang Zhao Shuai Liu Zeming Zhou Hongliang Yan Haining Li Tianyi Chen Xiaodong Xu Huiling Chen Huawei Zhang Jianrong Shi CAS Key Laboratory of Optical Astronomy National Astronomical ObservatoriesChinese Academy of SciencesBeijing 100101China School of Astronomy and Space Science University of Chinese Academy of SciencesBeijing 100049China Department of Astronomy School of PhysicsPeking UniversityBeijing 100871China Kavli Institute for Astronomy and Astrophysics Peking UniversityBeijing 100871China

Chemical composition of very metal-poor(VMP)stars can provide observational constraints on current models of nucleosynthesis and the chemical evolution of the *** has been found that the scatter of[Na/Fe]versus[Fe/H]in VMP stars is very large in contrast with most other ***,a negative slope in[Na/Fe]versus[Fe/H]was found for giants,which is very unlikely according to the theory of *** the sample of93 VMP stars in the metallicity range-4.25<[Fe/H]<-1.64 we obtained NLTE sodium abundances using the line profile fitting method by employing accurate atmospheric parameters determined when taking into account NLTE line formation for both FeⅠand FeⅡ.Originally selected from the LAMOST low-resolution spectral database,the spectra of stars were obtained with the High Dispersion Spectrograph of the Subaru *** 57turn-off stars in metallicity domain-3.04<[Fe/H]<-1.64,we obtained mean[Na/Fe]=-0.29±0.14 and positive slope 0.09±*** 21 giants distributed over metallicity-3.59<[Fe/H]<-2.19,we found mean[Na/Fe]=-0.35±0.1 and positive slope 0.07±***[Na/Fe]trend is lower by~0.2 dex,compared to the modern GCE *** believe the GCE model should be adjusted,by considering the associated *** stars in our sample are found to be outliers,with too low or too high Na abundances.

关键词： techniques:spectroscopic stars:abundances stars:atmospheres

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放射性液态流出物连续监测系统设计

核电子学与探测技术 2021年第3期41卷 382-385页

作者：沈福中国辐射防护研究院太原030006

针对核设施中液态流出物^(60)Co、^(137)Cs的测量,构建了基于Nal探测器的核索识别型放射性液态流出物监测系统。实验测试表明:该系统稳定可靠,具有核素识别能力,实现^(60)Co、^(137)Cs的探测限小于0.111 Bq/L。该仪器可用于流出物关键... 详细信息

针对核设施中液态流出物^(60)Co、^(137)Cs的测量,构建了基于Nal探测器的核索识别型放射性液态流出物监测系统。实验测试表明:该系统稳定可靠,具有核素识别能力,实现^(60)Co、^(137)Cs的探测限小于0.111 Bq/L。该仪器可用于流出物关键γ核素监测,还可用于饮用水关键核素在线监测。

关键词：放射性监测仪多道探测排放

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新型人工核素气溶胶监测仪设计

核电子学与探测技术 2020年第2期40卷 288-292页

作者：沈福中国辐射防护研究院太原030006

针对核设施人工放射性气溶胶与氡钍子体粒度具有明显差异,利用这种差异,设计了一种带有氡子体与人工放射性核素粒度分离、负压抽真空已达稀薄空气层的监测仪器。实现进一步降低天然辐射氡钍子体干扰,提高监测灵敏度。实验测试结果表明:... 详细信息

针对核设施人工放射性气溶胶与氡钍子体粒度具有明显差异,利用这种差异,设计了一种带有氡子体与人工放射性核素粒度分离、负压抽真空已达稀薄空气层的监测仪器。实现进一步降低天然辐射氡钍子体干扰,提高监测灵敏度。实验测试结果表明:以最小可探测活动衡量方式的探测灵敏度较无粒度分离装置提高2〜15倍;该技术在人工放射性与天然氡钍子体粒度有显著差异,氡钍子体水平越高,优势越明显。

关键词：氡钍子体高氡放射性气溶胶粒度分离真空

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新核电烟囱取样管路损失试验评估

核电子学与探测技术 2020年第3期40卷 392-395页

作者：沈福中国辐射防护研究院太原030006

针对CAP1400、台山、田湾等新核电厂烟囱气载放射性排放监测取样管线损失,本文采用实验的方法评估,在验室搭建了管路损失的实验装置,并就单位长度不锈钢1寸管,粗糙度为3.2μm的不锈钢管路开展了试验,为核电烟囱排放设施评估提供了数据支... 详细信息

针对CAP1400、台山、田湾等新核电厂烟囱气载放射性排放监测取样管线损失,本文采用实验的方法评估,在验室搭建了管路损失的实验装置,并就单位长度不锈钢1寸管,粗糙度为3.2μm的不锈钢管路开展了试验,为核电烟囱排放设施评估提供了数据支撑,相比过去无数据状态,提升了排放监测取样的有效性。

关键词：气载放射性排放年剂量限排放流监测剂量因子

Vitamin C Inhibits Benzo[a]pyrene-Induced Cell Cycle Changes Partly via Cyclin D1/E2F Pathway in Human Embryo Lung Fibroblasts

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Biomedical and Environmental Sciences 2006年第3期19卷 239-244页

作者： AI GAO BING-CI LIU XIANG-LIN SHI CHUAN-SHU HUANG XIAO-WEI JIA BAO-RONG YOU MENG YE fu-HAI shen HONG-JU DU National Institute of Occupation Health and Poison Control Chinese Center for Disease Control and Prevention Beijing 100050 China National Institute for Occupational Safety and Health 1095 Willowdale RoadMorgantown WV26506 USA Nelson Institute of Environmental Medicine New York University School of Medicine 57 Old Forge Road Tuxedo NY 10987

Objective To study the molecular mechanism of the inhibitory effects of vitamin C on benzo[a]pyrene （B[a]P）-induced changes of cell cycle in human embryo lung fibroblast （HELF） cells. Methods The stable transfectants, HELF transfected with antisense cyclin D1 and antisense CDK4, were established. Cells were cultured and pretreated with vitamin C before stimulation with B[a]P for 24 h. The expression levels of cyclin DI, CDK4, E2FI, and E2F4 were determined by Western blot. Flow cytometric analysis was employed to detect the distributions of cell cycle. Results B[a]P significantly elevated the expression levels of cyclin D 1, E2F1, and E2F4 in HELF cells. Vitamin C decreased the expression levels of cyclin D 1, E2F1, and E2F4 in B [a]P-stimulated HELF cells. Dose-dependent relationships were not found between the different concentrations of vitamin C （10, 100, 500, 1000, and 5000 lamol/L） and the expression levels of cyclin D 1, E2F1, and E2F4 in HELF cells. The expression levels of cyclin D1, E2FI, and E2F4 in B[a]P-treated transfectants were lower than those in B[a]P-treated HELF cells. The expression levels of cyclin DI and E2F4 treated with vitamin C and antisense cyclin D1 were decreased compared with those treated with antisense cyclin DI alone. The effects of vitamin C combined with antisense CDK4 on the expression levels of cyclin DI and E2FI/E2F4 were similar to those of antisense CDK4 alone. B[a]P progressed HELF cells from GI to S phase. Both vitamin C and antisense cyclin DI suppressed the changes of cell cycle progressed by B[a]P. However, antisense CDK4 did not attenuate the above changes. Vitamin C combined with antisense CDK4 markedly suppressed B[a]P-induced changes of cell cycle as compared with antisense CDK4. But the inhibitory effects of vitamin C combined with antisense cyclin DI on B[a]P-induced changes of cell cycle were similar to those of vitamin C alone or antisense cyclin DI alone. Conclusions B[a]P progressed HELF cells from G1 to S phase

关键词： B[a]P Ascorbic acid Cyclin D1 E2F Cell cycle Antisense