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多排螺旋CT冠状动脉血管成像检查在老年患者特殊护理中的临床意义

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中国医学创新 2015年第26期12卷 111-112页

作者：陈晓玲谢娟付华静广东省深圳市人民医院广东深圳518101

目的:探讨多排螺旋CT冠状动脉血管成像检查对于老年患者特殊护理的临床意义。方法:选取2014年1月-2015年3月来本院做体检的1282例老年患者,按照随机数字表法将其分为对照组和观察组,每组641例。对照组采用常规护理,观察组采用特殊护理,... 详细信息

目的:探讨多排螺旋CT冠状动脉血管成像检查对于老年患者特殊护理的临床意义。方法:选取2014年1月-2015年3月来本院做体检的1282例老年患者,按照随机数字表法将其分为对照组和观察组,每组641例。对照组采用常规护理,观察组采用特殊护理,观察两组老年患者多排螺旋CT冠状动脉血管成像检查的结果,分析其临床意义。结果:经护理后,观察组多排螺旋CT冠状动脉血管成像检查前后老年患者心率变化显著率、屏气情况不佳率、图像质量情况较差率分别为10.1%、6.7%和8.4%,均明显低于对照组的45.4%、31.7%和30.7%,差异均有统计学意义(P<0.05)。结论:对老年患者采用特殊护理后,可以减少老年患者的检查前后心率变化显著、屏气不佳以及图像质量较差现象,确保多排螺旋CT冠状动脉血管成像检查临床诊断效果的发挥,值得临床推广。

关键词：多排螺旋CT 冠状动脉血管成像检查老年患者护理

蓝藻在微塑料上定植并对海洋生态系统产生的影响

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环境生态学 2024年第5期6卷 41-49页

作者：刘浩林杨冰付京花华南农业大学海洋学院广东广州510642

近年来微塑料在海洋中的不断积累,引发了人们对海洋生态系统功能及生态多样性的关注,其中包括蓝藻在微塑料上定植所产生的影响。在微塑料上定植,会对蓝藻的生长和形态、光合作用和脂肪酸产生不同的影响。而蓝藻的定植则会改变微塑料的... 详细信息

近年来微塑料在海洋中的不断积累,引发了人们对海洋生态系统功能及生态多样性的关注,其中包括蓝藻在微塑料上定植所产生的影响。在微塑料上定植,会对蓝藻的生长和形态、光合作用和脂肪酸产生不同的影响。而蓝藻的定植则会改变微塑料的物化特性,同时还表现为降解微塑料或延缓光老化过程。通过定植作用,蓝藻与微塑料产生相互作用,影响海洋生态系统初级生产力的同时,通过食物链的逐级传递,对整个海洋生态系统产生影响。本研究对蓝藻定植于微塑料在海洋生态系统中的研究现状进行概述,强调未来需要进一步开展的研究工作,以更好地理解蓝藻的定植对彼此及整个生态系统的潜在影响,这将为制定有效的保护措施和管理策略提供重要的科学依据。

关键词：蓝藻浮游植物微塑料细菌定植海洋生态系统

Single-cell omics: experimental workflow, data analyses and applications

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Science China Life Sciences 2025年第1期68卷 5-102页

作者： Fengying Sun Haoyan Li Dongqing Sun Shaliu fu Lei Gu Xin Shao Qinqin Wang Xin Dong Bin Duan Feiyang Xing Jun Wu Minmin Xiao Fangqing Zhao jing-Dong J.Han Qi Liu Xiaohui Fan Chen Li Chenfei Wang Tieliu Shi Department of Clinical Laboratory the Affiliated Wuhu Hospital of East China Normal University (The Second People's Hospital of Wuhu City) Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang University Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration (Tongji University) Ministry of EducationOrthopaedic DepartmentTongji HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Frontier Science Center for Stem Cells School of Life Sciences and TechnologyTongji University Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine Shanghai East HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Research Institute of Intelligent Computing Zhejiang Lab Shanghai Research Institute for Intelligent Autonomous Systems Center for Single-cell Omics School of Public HealthShanghai Jiao Tong University School of Medicine National Key Laboratory of Chinese Medicine Modernization Innovation Center of Yangtze River DeltaZhejiang University Center for Bioinformatics and Computational Biology Shanghai Key Laboratory of Regulatory Biologythe Institute of Biomedical Sciences and School of Life SciencesEast China Normal University Beijing Institutes of Life Science Chinese Academy of Sciences Peking-Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary StudiesCenter for Quantitative Biology (CQB)Peking University Zhejiang Key Laboratory of Precision Diagnosis and Therapy for Major Gynecological Diseases Women's HospitalZhejiang University School of Medicine Key Laboratory of Advanced Theory and Application in Statistics and Data Science-MOE School of StatisticsEast China Normal University

Cells are the fundamental units of biological systems and exhibit unique development trajectories and molecular features. Our exploration of how the genomes orchestrate the formation and maintenance of each cell, and control the cellular phenotypes of various organismsis, is both captivating and intricate. Since the inception of the first single-cell RNA technology, technologies related to single-cell sequencing have experienced rapid advancements in recent years. These technologies have expanded horizontally to include single-cell genome, epigenome,proteome, and metabolome, while vertically, they have progressed to integrate multiple omics data and incorporate additional information such as spatial sc RNA-seq and CRISPR screening. Single-cell omics represent a groundbreaking advancement in the biomedical field,offering profound insights into the understanding of complex diseases, including cancers. Here, we comprehensively summarize recent advances in single-cell omics technologies, with a specific focus on the methodology section. This overview aims to guide researchers in selecting appropriate methods for single-cell sequencing and related data analysis.

关键词： single-cell sequencing genome epigenome proteomics metabolomics multimodal spatial transcriptomics CRISPR screening

MiR-36f对猪蛔虫幼虫感染力和发育的影响（英文）

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中国人兽共患病学报 2015年第11期31卷 1037-1041页

作者：冯胜勇付京花邵长春朱兴全徐民俊中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室兰州730046 华南农业大学动物科学院广州510642 扬州大学兽医学院扬州225009

目的探讨猪蛔虫虫卵和幼虫期特异表达的miR-36f的是否与猪蛔虫幼虫的感染性和发育有关。方法将合成的miR-36f的模拟物,抑制剂以及模拟物对照和抑制剂对照通过浸泡的方法被猪蛔虫三期幼虫吸收后,分别将这四组幼虫通过灌胃的方式感染小鼠... 详细信息

目的探讨猪蛔虫虫卵和幼虫期特异表达的miR-36f的是否与猪蛔虫幼虫的感染性和发育有关。方法将合成的miR-36f的模拟物,抑制剂以及模拟物对照和抑制剂对照通过浸泡的方法被猪蛔虫三期幼虫吸收后,分别将这四组幼虫通过灌胃的方式感染小鼠,以不作任何处理的幼虫感染小鼠作为空白对照组,四天后采用贝尔曼法收集各组小鼠肺和肝的幼虫。对各组回收幼虫的数量以及幼虫的长度和宽度进行测量,并采用qRT-PCR方法对miR-36f的两个靶基因OST48和cytochrome b进行定量分析。结果抑制miR-36f以后,miR-36f的两个靶基因OST48和cytochrome b均被下调,幼虫发育和感染力受到抑制。结论提示猪蛔虫的miR-36f与幼虫个体发育和感染力有关。

关键词：猪蛔虫 asu-miR-36f 感染力发育

Facile synthesis of the dodecahydridododecaborate (B12H122-) from borane Lewis base adducts

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Science China Chemistry 2025年

作者： Yi jing Xin-Ran Liu Xing-hua Wang Hui Han Sha-Sha Liang Xi-Meng Chen Dong-Hui Wei Lai-Sheng Wang Xue-Nian Chen College of Chemistry Zhengzhou University School of Chemistry and Chemical Engineering Henan Key Laboratory of Boron Chemistry and Advanced MaterialsHenan Normal University Department of Chemistry Brown University

Polyhedral boranes are a class of well-known boron molecular clusters widely used in energy,chemistry,medicine,and materials science because of their unique physical and chemical *** efforts have been made in the past decades to find more effective synthetic methods for this important class of boron ***,existing synthetic methods suffer from low efficiency and low ***,we report a facile one-pot synthesis of[（CH3）3S]2B12H12with moderate yields at mild *** mechanisms for the multi-step chemoselective synthesis of B12H122-without other by-products are elucidated based on theoretical results and our previous *** Lewis base used in B–H bond condensation reaction,which acts as a hydrogen or to balance the newly generated polyhedral borane charges,is proposed and studied in *** current study has led to a more effective and selective synthetic method for B12H122-and has also implicated the syntheses of other new polyhedral boranes.

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Sine oculis homeobox homolog family function in gastrointestinal cancer:Progression and comprehensive analysis

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World Journal of Clinical Oncology 2025年第1期16卷 10-24页

作者： Yang-Zheng Lan Zheng Wu Wen-Jia Chen Xin-Ning Yu hua-Tao Wu jing Liu Department of The Breast Center Cancer Hospital of Shantou University Medical CollegeShantou 515041Guangdong ProvinceChina Department of General Surgery First Affiliated Hospital of Shantou University Medical CollegeShantou 515041Guangdong ProvinceChina

The sine oculis homeobox homolog(SIX)family,a group of transcription factors characterized by a conserved DNA-binding homology domain,plays a critical role in orchestrating embryonic development and organogenesis across various organisms,including *** six distinct members,from SIX1 to SIX6,each member contributes uniquely to the development and differentiation of diverse tissues and organs,underscoring the versatility of the SIX *** or mutations in SIX genes have been implicated in a spectrum of developmental disorders,as well as in tumor initiation and progression,highlighting their pivotal role in maintaining normal developmental trajectories and cellular *** to target the transcriptional complex of the SIX gene family have emerged as a promising strategy to inhibit tumor *** the development of inhibitors targeting this gene family is still in its early stages,the significant potential of such interventions holds promise for future therapeutic ***,this review aimed to comprehensively explore the advancements in understanding the SIX family within gastrointestinal cancers,focusing on its critical role in normal organ development and its implications in gastrointestinal cancers,including gastric,pancreatic,colorectal cancer,and hepatocellular *** conclusion,this review deepened the understanding of the functional roles of the SIX family and explored the potential of utilizing this gene family for the diagnosis,prognosis,and treatment of gastrointestinal cancers.

关键词： Sine oculis homeobox homolog Gastrointestinal cancer Transcription factor Development Regulation Diagnosis Therapeutics

皱纹盘鲍外套膜耐维生素E缺乏消减cDNA文库的构建

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中国水产科学 2007年第3期14卷 383-389页

作者：刘晶张文兵麦康森付京花冯秀妮马洪明徐玮刘付志国中国海洋大学教育部海水养殖重点实验室中国海洋大学教育部海水养殖重点实验室山东青岛266003

为了克隆维生素E缺乏时皱纹盘鲍(Haliotis discus hannai)外套膜差异性表达的基因,本研究配制了维生素E缺乏水平(3.5 mg/kg)和正常添加水平(52.8 mg/kg)的人工饲料,喂养皱纹盘鲍幼鲍,初始体质量为(0.71±0.00)g,初始壳长为(15.49±0.04)... 详细信息

为了克隆维生素E缺乏时皱纹盘鲍(Haliotis discus hannai)外套膜差异性表达的基因,本研究配制了维生素E缺乏水平(3.5 mg/kg)和正常添加水平(52.8 mg/kg)的人工饲料,喂养皱纹盘鲍幼鲍,初始体质量为(0.71±0.00)g,初始壳长为(15.49±0.04)mm,240 d后,采用抑制消减杂交法(Suppression Subtractive Hybridization,SSH)构建了维生素E缺乏组与正常组外套膜组织差异表达的cDNA消减文库。经检验,差异表达的基因均被富集了25-28倍,证明构建的cDNA消减文库具有很强的消减效率。从文库中随机挑取100个白色克隆摇菌液进行PCR鉴定,95%的克隆中均有100-900 bp的插入片段,这些片段可能是维生素E缺乏差异表达基因的cDNA片段。选取含插入片段大小不同的48个克隆测序,其中有16个新基因,10个谷胱甘肽转移酶的基因,5个谷胱甘肽过氧化物酶基因,2个过氧化氢酶基因,3个羟基类固醇脱氢酶基因,4个酪氨酸激酶基因,1个类甲状腺过氧化物酶蛋白基因,3个纤维素酶基因,1个cAMP效应元件结合蛋白基因,3个贝壳生物矿化相关蛋白的基因。总的来说,利用抑制消减杂交的方法构建差异表达cDNA文库,可以比较好地反映维生素E对皱纹盘鲍影响的基因信息,为研究其他营养素对皱纹盘鲍基因表达的影响提供了基础数据。

关键词：皱纹盘鲍外套膜维生素E 抑制消减杂交 cDNA消减文库

Lipomatous ependymoma with ZFTA:RELA fusion-positive:A case report

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World Journal of Clinical Cases 2025年第1期13卷 48-55页

作者： Xiao-Yu Zhao Juan-Han Yu Yi-hua Wang Yi-Xin Liu Lu Xu Lin fu Ning Yi China Medical University The First Hospital of China Medical UniversityShenyang 110001Liaoning ProvinceChina Department of Pathology The First Hospital of China Medical UniversityShenyang 110001Liaoning ProvinceChina Anshan Cancer Hospital Anshan 114000Liaoning ProvinceChina

BACKGROUND Ependymoma with lipomatous differentiation is a rare type of *** ZFTA fusion-positive supratentorial ependymoma is a novel tumor type in the 2021 World Health Organization classification of central nervous system *** fusion-positive lipomatous ependymoma has not been reported to *** SUMMARY We reported a case of a 15-year-old Chinese male who had a sudden convulsion lasting approximately six *** resonance imaging showed a round cystic shadow of approximately 1.9 cm×1.5 cm×1.9 cm under the right parieto-occipital *** examination showed characteristic perivascular pseudorosettes and adipose differentiation in the ***-chemical staining showed that the tumor cells were negative for cytokeratin,NeuN,Syn and p53,but positive for GFAP,vimentin and S-100 ***-ficant punctate intracytoplasmic EMA immunoreactivity was *** level of Ki-67 was about 5%.Genetic analysis revealed ZFTA:RELA fusion.A cranio-tomy with total excision of the tumor was *** follow-up time was 36 months,no evidence of disease recurrence was found in magnetic resonance *** Based on these findings,the patient was diagnosed as a ependymoma with ZFTA fusion and lipomatous *** case report provides information on the microscopic morphological features of ependymoma with ZFTA fusion and lipomatous differentiation,which can help pathologists to make a definitive diagnosis of this tumor.

关键词： Ependymoma Lipomatous Molecular classification Supratentorial ZFTA:RELA fusion Case report

光测法与电测法应用于大型土木工程健康监测的对比试验研究

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工程质量 2006年第11期24卷 47-50页

作者：符晶华南秋明符蓉熊仁阶眭灵峰武汉理工大学光纤传感技术中心华中科技大学文华学院湖北建科结构加固有限责任公司

为实现对大型土木工程的健康监测,笔者在某实际工程的大跨混凝土梁进行静载试验之际,分别采用光测法(光纤布拉格光栅传感器)与电测法(电阻应变片)对梁进行了同步检测。结果表明,前者比后者具有更好的稳定性和可靠性,检测结果更加合理。... 详细信息

为实现对大型土木工程的健康监测,笔者在某实际工程的大跨混凝土梁进行静载试验之际,分别采用光测法(光纤布拉格光栅传感器)与电测法(电阻应变片)对梁进行了同步检测。结果表明,前者比后者具有更好的稳定性和可靠性,检测结果更加合理。因而光纤布拉格光栅传感器等光测法在大型土木工程健康检测方面具有更好的应用前景。

关键词：光纤光栅传感器健康监测大型土木工程