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Inhibitory effect of three biopolymer materials on scar formation following trabeculectomy

International Journal of Ophthalmology(English edition) 2011年第4期4卷 380-383页

作者： Sha, Qian Jiao, Guang-Yu Cui, Hai-bin Wang, Jie Sun, Li-bin Chen, Ming fu, song-bin Harbin Med Coll Harbin 150001 Heilongjiang Pr Peoples R China China Med Univ Shengjing Hosp Dept Ophthalmol Shenyang 110004 Liaoning Provin Peoples R China Eye Dis Prevent Inst Harbin 150001 Heilongjiang Pr Peoples R China Heilongjiang Prov Hosp Dept Ophthalmol Harbin 150001 Heilongjiang Pr Peoples R China

AIM: To investigate the inhibitory effects of amniotic membrane, polylactic acid membrane and chitosan membrane on scar formation following trabeculectomy. METHODS: A total of 24 New Zealand white rabbits (48 eyes) were randomly divided into 4 groups: amniotic membrane group, polylactic acid membrane group, chitosan membrane group, and control group, with 6 rabbits (12 eyes) in each group. The left eyes underwent routine trabeculectomy, and the right eyes were considered as controls. Amniotic membrane, polylactic acid membrane and chitosan membrane were respectively installed under sclera flap in three groups, but any treatment was not applied in control group. Intraocular pressure, conjunctival filtering bleb, and anterior chamber inflammation responses were monitored at day 1, 3, 7, 14, 28 and 56 post-operatively. Eyeball tissue underwent histopathological examination at day 56 post-operatively. RESULTS: Fibrocytes and inflammatory cells were reduced in amniotic membrane, polylactic acid membrane and chitosan membrane groups compared to that in control group. At day 1 post-operatively, intraocular pressure was decreased in three membrane groups compared to that in control group. At day 14 post-operatively, the intraocular pressure was decreased significantly, while it of three membrane groups was significantly lower than that of preoperative (P0.05). Filtering bleb of four groups was clearly observed at day 7 post-operatively, but there was no significant difference in pair-wise comparison. At day 28 and 56 post-operatively, filtering bleb in control group was significantly narrowed compared to that in three membrane groups (P <0.05), but there was no significant difference in pair-wise comparison of three membrane groups. CONCLUSION: All amniotic membrane, polylactic acid membrane and chitosan membrane can effectively inhibit scar formation following trabeculectomy, the effect of amnioti

关键词： amniotic membrane polylactic acid membrane chitosan membrane glaucoma filtration surgery

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Impact of Arsenic Trioxide on LS-174T Cell Growth in vitro and the Activity of Telomerase

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Chemical Research in Chinese Universities 2006年第2期22卷 209-212页

作者： WANG Xi-shan WANG Gui-yu XU Hai-tao DONG De-li fu song-bin ZHANG Qi-fan YANG Bao-feng Department of Abdominal Surgery the Affiated Tumor Hospital Department of Pharmacology Department of Genetics Harbin Medical University Harbin 150001 P. R. China

The therapeutic action of arsenic trioxide（ As203 ） on solid tumors has aroused widespread interest among scholars. To study the impact of As2O3 on human colorectal carcinoma cells（ LS-174T cell） and the activity of telomerase, the methods of PCR-ELISA, flow cytometry （FCM） and MTT assay in vitro were utilized. The results show that （1） with an increase in the concentration of As2O3, the ratio of living the cells to dead cells decreases significantly, and the IC50 value is 5.23 μg/mL; （2） the cells of the experimental groups can endure a series of morphological changes similar to the features of apoptosis ; （3） the apoptotic curves of FCM pictures appear after 24 h, and the cells show the apoptosis in a time-dependent manner; （4） As2O3 can inhibit the activity of telomerase of the cell extraction obviously in a concentration-dependent and time-dependent manner after 24 h. It can be concluded from the experiment results in vitro that As2O3 can induce the apoptosis of LS-174T cells and inhibit the telomerase activity. Therefore, it has been proposed, for the first time, that these two factors （the apoptosis of LS-174T cells and the inhibition to the telomerase activity） are important causes of the LS-174T cell death caused by As2O3.

关键词： Arsenic trioxide Telomerase Colorectal carcinoma Apoptosis

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Studies on Intramolecular Stacking Interaction of Ternary Complexes M（Ⅱ）（ATP）￣（2－） with Heteroaromatic N－Base Ligands

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Chemical Research in Chinese Universities 1994年第3期10卷 167-174页

作者： WU fu-liai, song bin, ZHANG Jie and JI Liang-nian (Chemistiy Department , Biotechnology Research Center, Zhongshan University, Guangzhou, 510275)

o understand the driving forces leading to mixed-ligand complexes in biologi-cal *** complexes of M (ATP(L￣(2-) type, where M = Cu￣(2+), Ni￣(2+) orCo￣(2+) , and L= pyridine(Py) , 4-picoline, 3, 5-lutidine, isoquinoline (lq) or benzimi-dazole(Bi) in aqueous solution were studied by spectropliotometry, some of them(M=Cu￣(2+), L = isoquinoline or benzimidazole) were also separately studied hy po-tentiometric pH titration. The results show that an intramolecular stacking interac-tion exists between the heteroaromatic ring of the ligands and the purine moiety ofATP.

关键词： Stacking interaction Ternary complexes Heteroaromatic N-base ATP

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Effects of cloned tumstatin-related and angiogenesis-inhibitory peptides on proliferation and apoptosis of endothelial cells

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Chinese Medical Journal 2008年第22期121卷 2324-2330页

作者： ZHANG Guang-mei ZHANG Ying-mei fu song-bin LIU Xing-han fu Xue YU Yan ZHANG Zhi-yi Department of Obstetrics & Gynaecology First Clinical College Harbin Medical University Harbin Heilongjiang 150001 China Central Laboratory First Clinical College Harbin Medical University Harbin Heilongjiang 150001 China Bio-pharmaceutical Key Laboratory of Heilongjiang Province Incubator of State Key Laboratory Basic College Harbin Medical University Harbin Heilongjiang 150086 China Department of Chemotherapy Third Clinical College Harbin Medical University Harbin Heilongjiang 150040 China Department of Rheumatology First Clinical College Harbin Medical University Harbin Heilongjiang 150001 China

Background Tumstatin is a recently developed endogenous vascular endothelial growth inhibitor that can be applied as an anti-angiogenesis and antineoplastic agent. The study aimed to design and synthesize the small molecular angiogenesis inhibition-related peptide （peptide 21）, to replicate the structural and functional features of the active zone of angiogenesis inhibition using tumstatin and to prove that synthesized peptide 21 has a similar activity： specifically inhibiting tumor angiogenesis like tumstatin. Methods Peptide 21 was designed and synthesized using biological engineering technology. To determine its biological action, the human umbilical vein endothelial cell line ECV304, the human ovarian cancer cell line SKOV-3 and the mouse embryo-derived NIH3T3 fibroblasts were used in in vitro experiments to determine the effect of peptide 21 on proliferation of the three cell lines using the MTT test and growth curves. Transmission electron microscopy （TEM） and flow cytometry （FCM） were applied to analyze the peptide 21-induced apoptosis of the three cell lines qualitatively and quantitatively. In animal experiments, tumor models in nude mice subcutaneously grafted with SKOV-3 were used to observe the effects of peptide 21 on tumor weight, size and microvessel density （MVD）. To initially investigate the role of peptide 21, the effect of peptide 21 on the expression of vascular endothelial growth factors （VEGFs） by tumor tissue was semi-quantitatively analyzed. Results The in vitro Ml-r test and growth curves all indicated that cloned peptide 21 could specifically inhibit ECV304 proliferation in a dose-dependent manner （P 〈0.01）; TEM and FCM showed that peptide 21 could specifically induce ECV304 apoptosis （P 〈0.01）. Results of in vivo experiments showed that tumors in the peptide 21 group grew more slowly. The weight and size of the tumors after 21 days of treatment were smaller than those in the control group （P 〈0.05）, with a mean tumor inhibition rate of 67.

关键词： tumstatin angiogenesis inhibitors anti-angiogenesis ovarian neoplasms

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Gene mapping of autosomal dominant retinitis pigmentosa in a Chinese family

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Chinese Medical Journal 2009年第9期122卷 1097-1101页

作者： DAI Li-li SUN Da-wei WANG Zheng fu song-bin HUANG Shang-zhi ZHANG Zhong-yu ZENG Guang PENG Shao-min Department of Ophthalmology Second Affiliated Hospital ofHarbin Medical University Harbin Heilongjiang 150081 China Genetic Laboratory Harbin Medical University HarbinHeilongjiang 150081 China Department of Medical Genetics Institute of Basic MedicalSciences Chinese Academy of Medical Sciences & Peking UnionMedical College Beijing 100005 China Departments of Surgery and Ophthalmology and Visual Sciences YaleUniversity School of Medicine New Haven CT USA

Background The autosomal dominant form of retinitis pigmentosa （ADRP） can be caused by mutations in 14 genes and further loci remains to be identified. This study was intended to identify mutations in a Chinese pedigree with ADRP. Methods A large Chinese family with retinitis pigmentosa was collected. The genetic analysis of the family suggested an autosomal dominant pattern. Microsatellite （STR） markers tightly linked to genes known to be responsible for ADRP were selected for linkage analysis. Exons along with adjacent splice junctions of PRPF31 were amplified by polymerase chain reaction （PCR） and screened by direct sequencing. Results The caused gene of ADRP was mapped to 19q13.4 between markers D19S572 and D19S877, with a maximum LOD score of 3.01 at marker D19S418 （recombination fraction=0）. Conclusion The affected gene linked to the 19q13.4 in a Chinese family with ADRP, which is different from other mutations at the same loci in other Chinese families.

关键词： retinitis pigmentosa, autosomal dominant genetic linkage Chinese

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人血清铜蓝蛋白水平变化及其意义的初步探讨

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中国地方病学杂志 2006年第2期25卷 168-169页

作者：崔晓波傅松滨哈尔滨医科大学医学遗传学教研室 150081

目的通过测定78例糖尿病患者及36例正常人血清铜蓝蛋白(ceruloplasmin,CP)水平,探讨CP 在糖尿病发生发展中的意义。方法将受试对象分为4组,分别采用免疫终点法测定血清CP。结果糖尿病组血清CP水平显著低于正常对照组(P0．05);2型单纯糖... 详细信息

目的通过测定78例糖尿病患者及36例正常人血清铜蓝蛋白(ceruloplasmin,CP)水平,探讨CP 在糖尿病发生发展中的意义。方法将受试对象分为4组,分别采用免疫终点法测定血清CP。结果糖尿病组血清CP水平显著低于正常对照组(P0．05);2型单纯糖尿病组与合并慢性并发症组血清CP 水平差异无统计学意义(P>0．10)。结论血清CP水平对于糖尿病患者的诊断及治疗效果的判断具有一定参考意义。

关键词：铜蓝蛋白糖尿病血糖

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第六次全国医学遗传学学术会议

作者： HUANG Yun JINYan fu song-bin Laboratory of Medical Genetics, Harbin Medical University, Harbin 150086, China

Annexin n is a Ca2+ -dependent phospholipid-binding protein that has been implicated in a number of membrane-related events. Annexin n has a unique N-terminus tail domain contains a nuclear export signal as well as multiple phosphorylation sites, suggesting annexin n in response to a wide range of stimuli is involved in cell growth regulation. In order to assess the exact function in cell proliferation, we focused on a

关键词： Annexin n. biological function. apoptosis

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BNIP3 Interact With p53 And Enhance Apoptosis of Lung Cancer mediated by p53

BNIP3 Interact With p53 And Enhance Apoptosis of Lung Cancer...

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第六次全国医学遗传学学术会议

作者： JINYan HUANG Yun YUYang fu song-bin Laboratory of Medical Genetics, Harbin Medical University, Harbin 150086, China

Our previous results suggest that exogenous wild type p53 can mediate apoptosis in lung cancer cells. It is known that in higher metastasis lung cancer, the speed of apoptosis is faster than in lower lung cancer, but the mechanism is unknown. We used cDNA apoptosis microchip to test the metastasis ability of four different cell lines(Anip973 and 95D, AGZY83-a and 95C). Our results demonstrated that the two first cell lines, Anip973 and 95D, have a higher metastasis ability, whereas the AGZY83-a and 95C, showed a lower

关键词： BNIP3. p53. Apoptosis

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自体骨骼肌细胞和血管内皮生长因子联合移植对坏死心肌的修复作用

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中国地方病学杂志 2007年第5期26卷 530-532页

作者：邓颖傅松滨王秀云哈尔滨医科大学附属第二医院急诊科 150081 哈尔滨医科大学生物学院 150081 哈尔滨医科大学附属第一医院 150081

目的探讨自体骨骼肌细胞和血管内皮生长因子（VEGF）联合移植对坏死心肌的修复作用。方法将成年兔24只随机分为4组：对照组、细胞移植组、VEGF组、细胞＋VEGF移植组。以体外培养兔臀骨骼肌细胞作为移植细胞。结扎兔左冠状动脉复制缺... 详细信息

目的探讨自体骨骼肌细胞和血管内皮生长因子（VEGF）联合移植对坏死心肌的修复作用。方法将成年兔24只随机分为4组：对照组、细胞移植组、VEGF组、细胞＋VEGF移植组。以体外培养兔臀骨骼肌细胞作为移植细胞。结扎兔左冠状动脉复制缺血坏死心肌模型，1周后分别注射培养液、含移植细胞的培养液、含VEGF的培养液、含移植细胞和VEGF的培养液。移植4周后，超声检查心脏功能，光镜观察坏死心肌形态结构。结果左心室射血分数对照组为（54．80±2．77）％，细胞移植组为（64．53±3．27）％，VEGF组为（55．23±2．78）％，细胞＋VEGF移植组为（66．83±3．25）％，组间比较差异有统计学意义（F=25．39，P〈0．05）。左室后壁收缩期末厚度对照组为（2．37±0．17）mm，细胞移植组为（3．32±0．23）mm，VEGF组为（2．48±0．28）mm，细胞＋VEGF移植组为（3．49±0．26）mm，组间比较差异有统计学意义（F：34．73，P〈0．05）。左室后壁舒张期末厚度对照组为（1．52±0．25）mm，细胞移植组为（2．47±0．13）mm。VEGF组为（1．60±0．16）mm，细胞＋VEGF移植组为（2．47±0．17）mm，组间比较差异有统计学意义（F=50．05，P〈0．05）。细胞＋VEGF移植组、细胞移植组分别与VEGF组和对照组比较，上述指标均明显增高（P〈0．05）。HE染色后，光镜下细胞＋VEGF移植组和细胞移植组的梗死区、梗死周边区均可见肌纤维，并有多核细胞生长。结论骨骼肌细胞与VEGF联合移植或单独移植均可对坏死心肌的心功能有明显改善作用。

关键词：肌骨骼细胞移植血管内皮生长因子梗死心肌

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Influence of Ambient-Temperature Corrosion on the Oxidation Behavior of Al-Si Coatings

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Chinese Journal of Aeronautics 2002年第4期15卷 244-249页

作者：张琦宋复斌王华阁骆军华 School of Materials Science and Engineering Beijing University of Aeronautics and Astronautics Beijing 100083 China Shenyang Aeronautics Engine Research and Design Institute Shengyang 110015 China

The high temperature oxidation behavior of Al Si coatings on the Ni base superalloy DZ125, with and without the ambient temperature corrosion test, was investigated. The results show that the isothermal oxidation behavior of the corroded coating changes, which has the higher mass gain than that of the original coating during the initial oxidation stage. Moreover, the cyclic oxidation resistance of the corroded coating decreases severely. The oxide scale was primarily composed of Al 2O 3, contaminated ...

关键词： ambient temperature corrosion oxidation Al Si coating

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