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The Brassica napus GATA transcription factor ***1 is a stigma compatibility factor

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Journal of Integrative Plant Biology 2020年第8期62卷 1112-1131页

作者： Zhiqiang Duan Yatao Zhang Jinxing Tu Jinxiong Shen Bin Yi tingdong fu Cheng Dai Chaozhi Ma National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070China

Self-incompatibility(SI)is a genetic mechanism that rejects self-pollen and thus prevents inbreeding in some hermaphroditic *** the Brassicaceae,SI involves a pollen-stigma recognition system controlled by a single locus known as the S locus,which consists of two highly polymorphic genes that encode S-locus cysteine-rich protein(SCR)and S-receptor kinase(SRK).When self-pollen lands on the stigma,the S-haplotype-specific interaction between SCR and SRK triggers ***,we show that the GATA transcription factor ***1 suppresses SI responses in Brassica napus and is induced after compatible *** loss-of-function mutant ***1 displays reduced *** contrast,overexpression of ***1 in self-incompatible stigmas leads to a partial breakdown of SI responses,suggesting that ***1 is a stigmatic compatibility ***,the expression levels of SRK and ARC1 are up-regulated in ***1 mutants,and they are down-regulated in ***1 overexpressing *** affects the cellular localization of ***1 through direct protein–protein ***,our findings highlight the fundamental role of ***1 in SI responses in ***,establishing a direct interaction between ***1 and SRK in this process.

关键词： interaction. compatibility establishing

Bna ***1,encoding a tetratricopeptide repeat protein,is required for early chloroplast biogenesis in Brassica napus

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The Crop Journal 2022年第3期10卷 597-610页

作者： Haiyan Zhang Xiaoting Li Yebitao Yang Kaining Hu Xianming Zhou Jing Wen Bin Yi Jinxiong Shen Chaozhi Ma tingdong fu Jinxing Tu National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070HubeiChina

Chloroplasts are essential for plant growth and development,as they play a key role in *** chloroplast biogenesis process is complex and its regulatory mechanism remains *** characterized a spontaneous Brassica napus(rapeseed)mutant,ytg,that showed a delayed greening phenotype in all green organs and retarded *** identified Bna ***1 encoding a chloroplastlocalized tetratricopeptide repeat protein widely expressed in rapeseed *** speculated that the ytg phenotype was caused by the deletion of Bna ***1 based on sequence comparison of 4608(with normal green leaves,isolated from the elite Chinese rapeseed cultivar ZS11)and ytg combined with transcriptome data and CRISPR/Cas9 gene editing *** homologous gene(Bna ***1)restored the phenotype of the *** ***1 interacted with MORF2,MORF8,and *** editing of the ndh D-2,ndh F-290,pet L-5,and ndh G-50 plastid transcripts was affected in *** findings suggested that Bna ***1 participates in RNA editing *** predicted 29 RNA editing sites in the chloroplast of Brassica napus by comparison with the Arabidopsis chloroplast *** conclude that Bna ***1 affects the posttranscriptional regulation of plastid gene expression and suggest that a tetratricopeptide repeat protein is involved in the chloroplast RNA editing in rapeseed.

关键词： Brassica napus Chloroplast MORFs RNA editing TPR

286份甘蓝型油菜种质苗期耐盐碱性综合评价

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植物遗传资源学报 2024年第3期25卷 356-372页

作者：薛天源鲁金春子何思晓余忆陈敬东文静沈金雄傅廷栋曾长立万何平江汉大学生命科学学院/湖北省汉江流域特色生物资源保护开发与利用工程技术研究中心武汉430056 华中农业大学植物科学技术学院/国家油菜工程技术研究中心武汉430070

对286份甘蓝型油菜品系进行苗期耐盐碱性鉴定,通过水培试验,测定盐碱胁迫处理下叶片数、绿叶数、绿叶比、株高、根长、根重等指标,通过盐碱胁迫综合评价值(D值)、极端材料筛选分析、相关性、主成分、隶属函数、频数分析和逐步回归分析法... 详细信息

对286份甘蓝型油菜品系进行苗期耐盐碱性鉴定,通过水培试验,测定盐碱胁迫处理下叶片数、绿叶数、绿叶比、株高、根长、根重等指标,通过盐碱胁迫综合评价值(D值)、极端材料筛选分析、相关性、主成分、隶属函数、频数分析和逐步回归分析法,对不同基因型的油菜种质建立苗期耐盐碱性综合评价模型并筛选出适宜的评价鉴定指标。盐碱胁迫下,叶片数与株高呈负相关,两者的相关系数未达到显著性,其他性状之间均呈正相关并达到了显著或极显著水平。利用主成分分析法将7个耐盐碱指标综合为4个主成分,可代表油菜耐盐碱性88.349%的原始数据信息量。依据4个主成分的相对重要性(权重)进行加权,得到不同基因型的耐盐碱性综合评价值(D值)。结合隶属函数分析和极端材料筛选分析,筛选出4份耐碱盐的甘蓝型油菜种质和4份盐碱敏感种质。逐步回归分析结果表明,在油菜苗期测定其绿叶数、绿叶比、地上重、根长和根重,通过回归方程估算其D值,可以初步判断甘蓝型油菜种质资源耐盐碱性。

关键词：甘蓝型油菜苗期盐碱胁迫种质资源筛选综合评价

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An in planta haploid induction system in Brassica napus

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Journal of Integrative Plant Biology 2022年第6期64卷 1140-1144页

作者： Yifan Li Dan Li Qing Xiao Huadong Wang Jing Wen Jinxing Tu Jinxiong Shen tingdong fu Bin Yi National Key Laboratory of Crop Genetic Improvement National Center of Rapeseed ImprovementHuazhong Agricultural UniversityWuhan 430070China Key Laboratory of Crop Physiology Ecology and Genetic BreedingMinistry of EducationJiangxi Agricultural UniversityNanchang 330045China

Doubled haploid technology is widely used to accelerate plant breeding,but its use in the important oilseed crop Brassica napus *** limited because *** haploids could only be obtained through in vitro anther or microspore ***,maize(Zea mays)lines containing mutations in Domain of unknown function 679 membrane protein(DMP)were used as haploid inducer *** new haploid induction mechanism has been extended to several other plants,including the dicots Arabidopsis thaliana,tomato(Solanum lycopersicum),and tobacco(Nicotiana tabacum).Here,we knocked out four BnaDMP genes in the *** cultivar Westar using a clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9 vector with an enhanced green fluorescent protein expression *** with DMP mutations in *** in the T_(0),T_(1),and T_(2) generations exhibited a haploid induction rate up to 2.53%.These results suggest that targeting BnaDMP could be useful for haploid induction in ***.

关键词： Brassica napus CRISPR/Cas9 fluorescence screening maternal haploid induction

*** affects chloroplast development in Brassica napus L.

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The Crop Journal 2021年第5期9卷 992-1002页

作者： Tingting Liu Baolong Tao Hanfei Wu Jing Wen Bin Yi Chaozhi Ma Jinxing Tu tingdong fu Lixia Zhu Jinxiong Shen National Key Laboratory of Crop Genetic Improvement National Center of Rapeseed ImprovementHuazhong Agricultural UniversityWuhan 430070HubeiChina School of Biological Science and Technology University of JinanJinan 250000ShandongChina

Mature chloroplasts,as the main sites of photosynthesis,are essential for seedling growth in higher *** of function of genes involved in chloroplast development changes plant *** obtained a YELLOW COTYLEDON (YCO) mutant in rapeseed (Brassica napus L.) using ***,a gene of unknown function,has two homologous copies (Bna *** and Bna ***) in *** mutation of these two homologs resulted in yellow cotyledons and chlorotic true *** electron microscopy revealed that the formation of thylakoid membranes was inhibited in yellow *** similarity search revealed that YCO was conserved in different species,and a subcellular location assay verified that *** was located in the *** was expressed in multiple tissues,most highly in *** of *** blocked the transcription of plastid genes,especially those of photosystem genes transcribed by plastid-encoded *** sequencing showed that the majority of genes involved in ribosome assembly and photosynthesis were down-regulated in *** *** results suggested that loss of function of *** affected plastid gene transcription,which influenced chloroplast biogenesis in rapeseed seedlings.

关键词： Brassica napus Chlorophyll biosynthesis Chloroplast biogenesis Chlorotic leaves Yellow cotyledon

Progress and future development of hybrid rapeseed in China

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Engineering Sciences 2013年第5期11卷 13-18页

作者： fu tingdong Zhou Yongming Huazhong Agricultural University

The hybrid growth of rapeseed now has reached to 4.7×106hm2,accounting for 70 % of total rapeseed growth area in China. Over the last 40 years,the researches and utilizations of hybrid rapeseed in China have significantly contributed to the development of rapeseed industry in China. The production of hybrid rapeseed mainly utilizes cytoplasmic male sterility(CMS),combined at the same time with nuclear male sterility and other pollinating-control systems in China. The hybrid rapeseed studies in China are also characterized by the improvement of quality and oil content in hybrid breeding. future studies to enhance the heterosis of rapeseed will be focused on several important issues,including the combination of heterosis and ideotype breeding,further increase of oil content in hybrids,utilization of sub-genomic heterosis and resistance improvement. The paper will discuss the following perspectives in hybrid rapeseed studies:relationships among heterosis,quality and disease traits,solutions for excessive source and pool in hybrids compared with open-pollinated cultivars,and the importance of increasing harvest index of hybrids to achieve a better yield in hybrids.

关键词： oil crops rapeseed heterosis plant breeding

A Mitochondria-Targeted PPR Protein Restores pol Cytoplasmic Male Sterility by Reducing orF224 Transcript Levels in Oilseed Rape

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Molecular Plant 2016年第7期9卷 1082-1084页

作者： Liu, Zhi Yang, Zonghui Wang, Xiang Li, Kaidi An, Hong Liu, Jie Yang, Guangsheng fu, tingdong Yi, Bin Hong, Dengfeng Huazhong Agr Univ Natl Ctr Rapeseed Improvement Wuhan Natl Key Lab Crop Genet Improvement Wuhan 430070 Peoples R China Huazhong Agr Univ Coll Plant Sci & Technol Wuhan 430070 Peoples R China

Cytoplasmic male sterility （CMS） in plants is a maternally inherited inability to produce functional pollen. CMS is generally caused by aberrant mitochondrial genes that are often chimeric in structure and frequentl... 详细信息

关键词： inability mitochondrial transcribed

黄籽甘蓝型油菜的合成和不同颜色种皮比较代谢组分析

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分子植物育种 2024年第12期22卷 3979-3987页

作者：杨芳杰叶沈华马晓伟陈延平易斌马朝芝沈金雄涂金星傅廷栋文静华中农业大学植物科学技术学院国家油菜工程技术研究中心武汉430070

为分析人工合成甘蓝型油菜的稳定性以及黄黑籽种皮中的代谢物差异,本研究以一个A_(4)代甘蓝型油菜人工合成种为材料,调查其细胞学稳定性,对其自交A10代黄、黑籽株系进行了种皮香草醛染色和广泛靶向代谢组分析,结果表明人工合成甘蓝型油... 详细信息

为分析人工合成甘蓝型油菜的稳定性以及黄黑籽种皮中的代谢物差异,本研究以一个A_(4)代甘蓝型油菜人工合成种为材料,调查其细胞学稳定性,对其自交A10代黄、黑籽株系进行了种皮香草醛染色和广泛靶向代谢组分析,结果表明人工合成甘蓝型油菜A_(4)代株系具有稳定的38条染色体,有丝分裂和减数分裂行为正常。A_(10)代黄籽和黑籽株系种皮颜色差异与原花青素差异积累有关,黑籽材料从开花后30 d开始,原花青素逐步在种皮中积累,而黄籽种皮中几乎没有原花青素积累。授粉后50 d的黄、黑籽种皮中共检测出23类737种代谢物,其中差异代谢物343种。与黑籽种皮相比,黄籽种皮中分别有149种和194种代谢物显著上调和下调积累。差异代谢物显著富集在黄酮和黄酮醇生物合成路径、甘油酯代谢路径和花青素生物合成途径这3条代谢通路中。综上所述,该人工合成甘蓝型油菜黄籽和黑籽种皮颜色差异主要由原花青素等类黄酮物质的差异积累导致。研究结果为揭示甘蓝型油菜种皮颜色形成机理奠定了理论基础,为黄籽油菜品种选育提供了材料支持。

关键词：甘蓝型油菜人工合成种黄籽代谢组学原花青素种皮颜色

A light microscopy analysis of the microspore and tapetum development in male sterile rapeseed(Brassica napus) induced by tribenuron-methyl

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A light microscopy analysis of the microspore and tapetum de...