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Mapping of QTLs and candidate genes associated with multiple phenotypic traits for Huanglongbing tolerance in citrus

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Horticultural Plant Journal 2023年第4期9卷 705-719页

作者： Ming Huang Mikeal L. Roose Qibin Yu Ed Stover David g. Hall Zhanao Deng frederick g.gmitter jr. Key Laboratory of Plant Hormones and Development Regulation of Chongqing College of Life SciencesChongqing UniversityChongqing 401331China Citrus Research and Education Center University of FloridaLake AlfredFL 33850United States Department of Botany and Plant Sciences University of CaliforniaRiversideCA 92521United States United States Horticultural Research Laboratory Agricultural Research ServiceUnited States Department of AgricultureFort PierceFL 34945United States gulf Coast Research and Education Center University of FloridaWimaumaFL 33598United States

Huanglongbing(HLB)is the most devastating disease for citrus *** Liberibacter asiaticus(C Las),vectored by Asian citrus psyllid(ACP,Diaphorina citri Kuwayama),is the most common pathogen causing the *** citrus varieties are highly susceptible to HLB,whereas trifoliate orange(Poncirus trifoliata)is considered highly tolerant to *** F1 segregating population and their parent trifoliate orange and sweet orange,which had been exposed to intense HLB pressure for three years,was evaluated for disease symptoms,ACP colonization,C Las titer and tree vigor repeatedly for two to three *** orange and sweet orange showed significant differences for most of the phenotypic traits,and the F1 population exhibited a large variation.A high-density SNP-based genetic map with 1402 markers was constructed for trifoliate orange,which exhibited high synteny and high coverage of its reference genome.A total of 26 quantitative trait locus(QTLs)were identified in four linkage groups Lg-t6,Lg-t7,Lg-t8 and Lg-t9,of which four QTL clusters exhibit a clear co-localization of QTLs associated with different *** genome-wide analysis of gene expression in response to C Las infection in‘Flying Dragon’and‘Larger-Flower DPI-50-7’trifoliate orange,85 differentially expressed genes were found located within the QTL *** them,seven genes were classified as defense or immunity protein which exhibited the highest transcriptional change after C Las *** results indicate a quantitative genetic nature of HLB tolerance and identified candidate genes that should be valuable for searching for genetic solutions to HLB through breeding or genetic engineering.

关键词： Candidatus liberibacter QTL mapping Phenotyping F1 population Poncirus Citrus

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Rationale for reconsidering current regulations restricting use of hybrids in orange juice

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Horticulture Research 2020年第1期7卷 2375-2381页

作者： Ed Stover frederick g.gmitter jr.Jude grosser Elizabeth Baldwin guohong Albert Wu Jinhe Bai Yu Wang Peter Chaires Juan Carlos Motamayor USDA/ARS US Horticultural Research Lab.2001 S Rock Rd.Ft.PierceFL 34945USA Citrus Research and Education Center Univ.Florida700 Experiment Station Rd.Lake AlfredFL 33850USA US Department of Energy Joint Genome InstituteLawrence Berkeley National Laboratory1 Cyclotron RoadBerkeleyCA 94720USA New Varieties Development&Management Corp. PO Box 941058MaitlandFL 32794USA The Coca-Cola Company 2651 Orange Ave.ApopkaFL 32703USA

Huanglongbing(HLB)is a disease that has devastated the Florida citrus industry,threatens the entire *** industry,and globally is rapidly ***’s citrus production is 90%sweet orange,which is quite sensitive to *** heavy reliance on sweet orange for Florida citrus production makes the industry especially vulnerable to diseases that are damaging to this type of ***,90%of Florida oranges are used in producing orange juice that is defined by a federal regulation known as the“orange juice standard”,specifying that at least 90%of“orange juice”must be derived from Citrus *** analyses definitively reveal that sweet orange is not a true species,but just one of many introgression hybrids of *** and ***,with phenotypic diversity resulting from accumulated mutations in this single hybrid,the“sweet orange”.No other fruit industry is limited by law to such a narrow genetic ***,there are new citrus hybrids displaying reduced sensitivity to HLB,and in some cases they produce juice,alone or in blends,that consumers would recognize as“orange juice”.Reconsidering current regulations on orange juice standards may permit use of such hybrids in“orange juice”,providing greater latitude for commercialization of these hybrids,leading to higher-quality orange juice and a more sustainable Florida orange juice industry.

关键词： orange Rational stated

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柑桔线虫抗性主基因座Tyr1的特异标记开发与遗传作图改进(英文)

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分子植物育种 2009年第3期7卷 497-504页

作者：向旭邓占鳌郑启发陈存贤 frederick g.gmitter jr 广东省农业科学院果树研究所广州510640 美国佛罗里达大学海岸研究与教育中心美国佛罗里达大学柑桔研究与教育中心

本文利用先期从BAC文库获得的NBS-LRR类候选抗病基因克隆序列Pt8a和Pt9a,进一步开发与柑桔线虫抗性主效基因位点Tyr1连锁的分子标记。以Pt8a和Pt9a序列作探针,通过高密度克隆印迹杂交,从BAC文库筛选出200个以上的阳性克隆,以阳性克隆插... 详细信息

本文利用先期从BAC文库获得的NBS-LRR类候选抗病基因克隆序列Pt8a和Pt9a,进一步开发与柑桔线虫抗性主效基因位点Tyr1连锁的分子标记。以Pt8a和Pt9a序列作探针,通过高密度克隆印迹杂交,从BAC文库筛选出200个以上的阳性克隆,以阳性克隆插入序列设计引物,对柑桔抗线虫材料和感线虫材料开展以PCR扩增为基础的集群分离分析,发现一部分克隆序列与柑桔线虫抗性主效基因位点Tyr1紧密连锁;再通过染色体步行测序,分别从3个克隆(7A4,4L17和29F20)获得3个完整的NBS-LRR类候选抗病基因序列。从此类序列开发更高特异性的分子标记,并在利用原有分子标记的基础上,对柑桔线虫抗性杂交后代群体(9145family)构建较高密度的遗传图谱;同时,将新开发的分子标记应用于柑桔衰退病抗性杂交后代群体(9401family),以初步估算柑桔线虫抗性主效基因位点Tyr1与柑桔衰退病抗性基因Ctv的遗传距离。本文利用先期从BAC文库获得的NBS-LRR类候选抗病基因克隆序列Pt8a和Pt9a,进一步开发与柑桔线虫抗性主效基因位点Tyr1连锁的分子标记。以Pt8a和Pt9a序列作探针,通过高密度克隆印迹杂交,从BAC文库筛选出200个以上的阳性克隆,以阳性克隆插入序列设计引物,对柑桔抗线虫材料和感线虫材料开展以PCR扩增为基础的集群分离分析,发现一部分克隆序列与柑桔线虫抗性主效基因位点Tyr1紧密连锁;再通过染色体步行测序,分别从3个克隆(7A4,4L17和29F20)获得3个完整的NBS-LRR类候选抗病基因序列。从此类序列开发更高特异性的分子标记,并在利用原有分子标记的基础上,对柑桔线虫抗性杂交后代群体(9145family)构建较高密度的遗传图谱;同时,将新开发的分子标记应用于柑桔衰退病抗性杂交后代群体(9401family),以初步估算柑桔线虫抗性主效基因位点Tyr1与柑桔衰退病抗性基因Ctv的遗传距离。

关键词：柑桔线虫抗性基因分子标记遗传作图

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A method for the production and expedient screening of CRISPR/Cas9-mediated non-transgenic mutant plants

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Horticulture Research 2018年第1期5卷 774-785页

作者： Longzheng Chen Wei Li Lorenzo Katin-grazzini Jing Ding Xianbin gu Yanjun Li Tingting gu Ren Wang Xinchun Lin Ziniu Deng Richard J.McAvoy frederick g.gmitter jr. Zhanao Deng Yunde Zhao Yi Li Department of Plant Science and Landscape Architecture University of ConnecticutStorrsCTUSA Institute of Vegetable Crops Jiangsu Academy of Agricultural SciencesNanjingChina College of Horticulture and State Key Laboratory of Crop genetics and germplasm Enhancement Nanjing Agricultural UniversityNanjingChina State Key Laboratory of Subtropical Silviculture Zhejiang Agriculture and Forestry UniversityZhejiangHangzhouChina College of Horticulture Hunan Agricultural UniversityHunanChangshaChina Citrus Research and Education Center University of FloridaLake AlfredFLUSA Department of Environmental Horticulture Gulf Coast Research and Education CenterIFASUniversity of FloridaWimaumaFLUSA Section of Cell and Developmental Biology University of California at San DiegoSan DiegoCA92093USA

Developing CRISPR/Cas9-mediated non-transgenic mutants in asexually propagated perennial crop plants is challenging but highly ***,we report a highly useful method using an Agrobacterium-mediated transient CRISPR/Cas9 gene expression system to create non-transgenic mutant plants without the need for sexual *** have also developed a rapid,cost-effective,and high-throughput mutant screening protocol based on Illumina sequencing followed by high-resolution melting(HRM)*** tetraploid tobacco as a model species and the phytoene desaturase(PDS)gene as a target,we successfully created and expediently identified mutant plants,which were verified as tetra-allelic *** produced pds mutant shoots at a rate of 47.5%from tobacco leaf explants,without the use of antibiotic *** these pds plants,17.2%were confirmed to be non-transgenic,for an overall non-transgenic mutation rate of 8.2%.Our method is reliable and effective in creating non-transgenic mutant plants without the need to segregate out transgenes through sexual *** method should be applicable to many economically important,heterozygous,perennial crop species that are more difficult to regenerate.

关键词： transgenic transient fir

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The Importance of Posterior Tibial Slope in TKR: Pre and Post-Operative Measurements in United States Osteoarthritic Patients

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Open Journal of Orthopedics 2024年第8期14卷 374-379页

作者： frederick F. Buechel Sr frederick F. Buechel jr Rutgers New Jersey Medical School Department of Orthopaedic Surgery Newark USA Lenox Hill Hospital Department of Orthopaedic Surgery New York USA

Background: Sagittal plane placement of the tibial component in total knee replacement (TKR) has important implications for maximizing the range of flexion motion, allowing collateral ligaments to function more normally, as well as providing ideal compressive loading on the tibial bone-prosthesis interface. This study attempts to quantify the normal posterior tibial slope (PTS) angle pre-operatively and post-operatively in osteoarthritic patients after using a conventional extramedullary tibial resection guide to assess its effectiveness. Methods: Forty-nine primary cementless total knee replacements in 34 osteoarthritic patients were measured radiographically pre-operatively and one year post-operatively to determine the PTS and its effect on range of motion. Lateral X-rays, using the anterior cortical line of the tibia, were employed for all measurements. Results: Pre-operative PTS measured 11.83˚ (range 5˚ - 18˚), while post-operative PTS of implanted tibial components measured 11.30o (range 4˚ - 18˚). The pre-operative range of motion of 112˚ (range 30˚ to 135˚) was improved to 119˚ (range 90˚ to 135˚) post-operatively after 1 year. Conclusions: Anterior tibial shaft referencing using a conventional extramedullary tibial resection guide provides an easy and convenient method for reproducing the anatomical PTS during TKR. This methodology provided improvement in average range of motion from 112˚ pre-operatively to 119˚ post-operatively at one year.

关键词： Posterior Tibial Slope Posterior Tibial Inclination Extramedullary Tibial Resection guide Total Knee Alignment

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Reprogramming of a defense signaling pathway in rough lemon and sweet orange is a critical element of the early response to‘Candidatus Liberibacter asiaticus’

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Horticulture Research 2017年第1期4卷 62-76页

作者： Qibin Yu Chunxian Chen Dongliang Du Ming Huang Jiqiang Yao Fahong Yu Ronald H Brlansky frederick g.gmitter jr University of Florida Institute of Food and Agricultural SciencesCitrus Research and Education CenterLake Alfred33850FLUSA USDA ARSSEFTNRL21 Dunbar RoadByron31008GAUSA Interdisciplinary Center for Biotechnology Research University of Florida2033 Mowry RoadGainesville32611FloridaUSA

Huanglongbing(HLB)in citrus infected by Candidatus Liberibacter asiaticus(CLas)has caused tremendous losses to the citrus *** resistant genotypes have been identified in citrus species or close *** citrus varieties,rough lemon(Citrus jambhiri)has been considered tolerant due to its ability to produce a healthy flush of new growth after *** difference between tolerance and susceptibility is often defined by the speed and intensity of a plant’s response to a pathogen,especially early defense ***-seq data were collected from three biological replicates of CLas-and mock-inoculated rough lemon and sweet orange at week 0 and 7 following *** analysis of the differentially expressed genes(DEgs)indicated that genes involved in the mitogen activated protein kinase(MAPK)signaling pathway were highly upregulated in rough *** induces the transcription of WRKY and other transcription factors which potentially turn on multiple defense-related genes.A Subnetwork Enrichment Analysis further revealed different patterns of regulation of several functional categories,suggesting DEgs with different functions were subjected to *** general,the amplitude of the expression of defense-related genes is much greater in rough lemon than in sweet orange.A quantitative disease resistance response may contribute to the durable tolerance level to HLB observed in rough lemon.

关键词： citrus lemon orange

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Provenance Documentation to Enable Explainable and Trustworthy AI:A Literature Review

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Data Intelligence 2023年第1期5卷 139-162页

作者： Amruta Kale Tin Nguyen frederick C.Harris jr. Chenhao Li Jiyin Zhang Xiaogang Ma Department of Computer Science Universityof IdahoMoscowID83844USA Department of Computer Science and Engineering University of NevadaRenoRenoNV 89557USA

Recently artificial intelligence(AI)and machine learning(ML)models have demonstrated remarkable progress with applications developed in various *** is also increasingly discussed that AI and ML models and applications should be transparent,explainable,and ***,the field of Explainable AI(XAI)is expanding *** holds substantial promise for improving trust and transparency in AI-based systems by explaining how complex models such as the deep neural network(DNN)produces their ***,many researchers and practitioners consider that using provenance to explain these complex models will help improve transparency in AI-based *** this paper,we conduct a systematic literature review of provenance,XAI,and trustworthy AI(TAI)to explain the fundamental concepts and illustrate the potential of using provenance as a medium to help accomplish explainability in AI-based ***,we also discuss the patterns of recent developments in this area and offer a vision for research in the near *** hope this literature review will serve as a starting point for scholars and practitioners interested in learning about essential components of provenance,XAI,and TAI.

关键词： Explainable AI Trustworthy AI Provenance documentation Workflow platforms Data science

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Elimination of ESD Events and Optimizing Waterjet Deflash Process for Reduction of Leakage Current Failures on QFN-mr Leadframe Devices

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Journal of Electrical Engineering 2018年第4期6卷 238-243页

作者： frederick Ray I.gomez Tito T.Mangaoang jr. Back-End Manufacturing & Technology STMicroelectronics Inc. Calamba City Laguna 4027 Philippines

This technical paper presents the resolution of high leakage current failures on QFN-mr （Quad-Flat No-leads Multi-Row） LF （leadframe） devices by optimizing the waterjet deflash process and eliminating the ESD （electrostatic discharge） events. ESD damage to units can cause permanent or latent product failures which results in low final test yield, and worse, possible external customer complaints. The use of CO2 （carbon dioxide） bubbler was able to reduce the DI （deionized） water’s equivalent resistivity from 17 M？ to 0.30 M？, minimizing the tribocharging effect produced during the waterjet deflash process. Moreover, ESD events were eliminated by grounding the floating assembly equipment parts and installing appropriate ESD controls. It is of high importance to reduce or eliminate the leakage current failures to ensure the product quality, especially as the market becomes more demanding. After the optimization and implementation of the corrective and improvement actions, high leakage current occurrence was significantly reduced from baseline of 5,784 ppm to 20 ppm.

关键词： Leakage current failure ESD QFN-mr LF waterjet deflash process.

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Cementless Semi-Constrained Rotating Platform Total Knee Replacement: A Concise Follow-Up of a Previous Report at a Minimum of Twenty Years

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Open Journal of Orthopedics 2023年第3期13卷 71-77页

作者： frederick F. Buechel Sr. frederick F. Buechel jr. Thomas E. Helbig Rutgers NJ Medical School Newark USA South Mountain Orthopaedic Associates South Orange USA Lenox Hill Hospital New York USA

Background: A previous report evaluated the initial 310 cementless, Buechel-Pappas (B-P), Semi-Constrained Rotating Platform total knee replacements in 257 patients followed for an average of 7.6 years, range 2 - 18 years. Diagnoses were osteoarthritis in 233 patients, post traumatic arthritis in 4 patients and rheumatoid arthritis in 22 patients. Knee Scores, using a strict knee scoring scale were 86.4% excellent, 12.3% good, 0.3% fair and 1.0% poor results. Survivorship, using an end point of revision for any mechanical reason (including component loosening, bearing wear and bearing dislocation) was 99.4%. Survivorship for a poor knee score (including persistent pain, loosening, instability and infection was 97.6%. The purpose of the present study is to report the updated results of this same patient cohort at a minimum follow-up of twenty years. Methods: The average age of patients at the time of surgery was 67 years, range 34 to 91 years. A total of 20 patients (22 knees) were still living, with a follow-up of 20 - 30 years (mean 23.47 years). The same strict knee scoring scale and survivorship analysis were used to evaluate patient outcomes at the 20 to 30 years interval. Results: Survivorship, in the current study, using the same end points as in the previous study, was 96.5% at the 20 and 30-year intervals. Late mechanical failure and osteolysis were not identified. Conclusion: This cementless, semi-constrained rotating platform total knee replacement has stood the test of time for more than 20 years and can be considered acceptable for long-term use, in properly selected patients.

关键词： Knee Replacement Cementless Semi-Constrained Rotating

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巨猿、化石人和智人牙齿的波动不对称:人类进化中压力源变化的意义(英文)

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心理学报 2007年第3期39卷 489-494页

作者： Michael J. frederick gordon g. gallup jr University at Albany

波动不对称(FA)是压力的随机性引发双侧完美对称偏离的现象,这是个体无力免受发展中不利因素(如早期营养不良)侵害的一个标志。比较个体口腔两侧牙齿的大小是一种测量 FA 的方法。本研究汇集了 10 个物种共 296 名个体的牙齿测量结果,... 详细信息

波动不对称(FA)是压力的随机性引发双侧完美对称偏离的现象,这是个体无力免受发展中不利因素(如早期营养不良)侵害的一个标志。比较个体口腔两侧牙齿的大小是一种测量 FA 的方法。本研究汇集了 10 个物种共 296 名个体的牙齿测量结果,测量对象包括黑猩猩(学名 Pan troglodytes)、猩猩(学名Pongo pygmaeus)、大猩猩(学名 gorilla gorilla)、智人(学名 Homo sapiens),以及许多化石人。分析发现,猩猩样本牙齿的 FA 水平要显著低于大猩猩、黑猩猩、直立人、尼安德特人和智人样本的 FA 水平。而与之相反的是,智人与尼安德特人样本的牙齿 FA 水平要显著高于其它任何一种巨猿样本的 FA 水平。该文提出了有关缓和的选择压力的解释。

关键词：波动对称选择压力化石人

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