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Effects of "Candidatus Liberibacter solanacearum"(haplotype B)on Bactericera cockerelli fitness and vitellogenesis

Insect Science 2020年第1期27卷 58-68页

作者： Angelica Albuquerque Tomilhero Frias freddy Ibanez Azucena Mendoza William Mario de Carvalho Nunes Cecilia Tamborindeguy Department of Entomology Texas A&M University412 Heep CenterCollege StationTexasUSA DEM-Depto.de Agronomia Nucleo de Pesquisa em Biotecnologia AplicadaMaringaBrazil

"Candidatus Liberibacter solanacearum"(Lso)are phloem-restricted and unculturable Gram-negative *** five haplotypes have been identified worldwide;but only haplotypes A and B are associated with the vector Bactericera cockerelli(Sulc.)in the *** studies showed that Lso-infection reduces *** reproductive output and that Lso haplotype B is more pathogenic than Lso haplotype *** understand the interaction of Lso haplotype B and ***,the fitness of Lso-free and Lso B-infected insects,and the expression of vitellogenin(5cKg7-like),a gene involved directly in the insect reproduction were *** differences in the number of eggs oviposited,and the total number of progeny nymphs and adults were found among crosses of insects with or without *** differences in sex proportions were found between Lso B-infected and Lso-free crosses:a higher proportion of Fi adult females were obtained from Lso B-infected mothers.A significant reduction of BcVgl-like was observed in crosses performed with Lso B-infected females compared to the Lso-free *** female cohorts of different age,a significant reduction of BcVgl-like expression was measured in 7-d-old Lso B-infected females(virgin and mated)compared with 7-d-old Lso-free females(virgin and mated),*** reduction of BcVgl-like transcript was associated with a lower number of developing oocytes observed in female5s reproductive ***,this study represents the first step to understand the interaction of Lso B with ***,highlighting the effect of Lso B infection on egg production,BcVgl-like expression,and oocyte development.

关键词： feminization insect-pathogen interaction oviposition psyllids reproduction vitellogenesis

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Electrocardiographic markers of cardiac resynchronization therapy response:delayed time to intrinsicoid deflection onset in lateral leads

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Journal of Geriatric Cardiology 2022年第1期19卷 21-30页

作者： Rubén KA Tapia-Orihuela S Michael Gharacholou Samuel J Asirvatham freddy Del-Carpio Munoz Department of Medicine Universidad Nacional Mayor de San MarcosFacultad de Medicina de San FernandoLimaPeru Department of Cardiovascular Diseases Mayo ClinicJacksonvilleFLUSA Department of Cardiovascular Diseases Mayo ClinicRochesterMNUSA

Cardiac resynchronization therapy(CRT)has emerged as an important intervention for patients with heart failure(HF)with reduced ejection fraction and delayed ventricular *** these patients,CRT has demonstrated to improve quality of life,promote reverse left ventricular(LV)remodeling,reduce HF hospitalizations,and extend ***,despite advancements in our understanding of CRT,a significant number of patients do not respond to this *** invasive and non-invasive parameters have been assessed to predict response to CRT,but the electrocardiogram(ECG)has remained as the prevailing screening method albeit with ***,an accurate,simple,and reproducible ECG marker or set of markers would dramatically overcome the current *** describe the clinical utility of an old ECG parameter that can estimate ventricular activation delay:the onset to intrinsicoid deflection(ID).Based on the concept of direct measurement of ventricular activation time(intrinsic deflection onset),time to ID onset measures on the surface ECG the time that the electrical activation time takes to reach the area subtended by the corresponding surface ECG *** on this principle,the time to ID on the lateral leads can estimate the delay activation to the lateral LV wall and can be used as a predictor for CRT response,particularly in patients with non-specific intraventricular conduction delay or in patients with left bundle branch block and QRS<150 *** aim of this review is to present the current evidence and potential use of this ECG parameter to estimate LV activation and predict CRT response.

关键词： deflection intrinsic synchronization

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Cutaneous leishmaniasis control in Alta Verapaz (northern Guatemala): evaluating current efforts through stakeholders' experiences

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Infectious Diseases of Poverty 2021年第3期10卷 14-25页

作者： Renata Mendizabal-Cabrera Isabel Perez Victor Becerril Montekio freddy Perez Erick Duran Mei L.Trueba Center for Health Studies(CHS) Universidad del Valle de Guatemala(UVG)18 Avenida 11-95zona 15V.H.3Guatemala CityGuatemala Instituto Nacional de Salud Publica(INSP) CuernavacaMexico Communicable Diseases and Environmental Determinants of Health Department Pan American Health OrganizationWashingtonDCUSA. Leishmaniasis Sub-Program National Ministry of Health of GuatemalaGuatemala CityGuatemala Department of Global Health and Infection(GHI) Brighton and Sussex Medical School(BSMS)University of SussexBrightonUK.

Background:Cutaneous leishmaniasis(CL),endemic in Guatemala,mostly affects poor people living in the northern region.A national control program that ineludes surveillanee,diagnose,and treatment offered free of cost by the Ministry of Health(MoH)has been in place since ***,the incidence is increasing and treatment rates are not optimal,suggesting that current efforts are not being *** study aimed to understand barriers and facilitators of CL control in Guatemala as experienced and perceived by key stakeholders in order to comprehend what works well and does not and suggest evidence-informed ***:The study was conducted in the Coban municipality,the most endemic of Guatemala,situated in the Department of Alta *** were collected during May and June 2019 via focus groups and semi-structured interviews with key stakeholders,including local and national health personnel and residents of four communities of the endemic *** and content analysis of the collected data was conducted using ***:Three overarching issues hamper the effectiveness of current CL efforts:resource scarcity,treatment challenges,and knowledge-action *** economic resources from the MoH and community residents negatively impact incidence,detection of cases and treatment rates in that preventive action is insufficient and healthcare access is *** addition,local health workers often lack specialized CL training and access to the national CL control *** regards to the population living in the study area,misunderstanding of disease causation,shame associated with CL lesions,treatment pain fear,and long(often uncertain)waiting times for diagnose and treatment negatively affect people's willingness to seek help,treatment adherence,and their trust on the healthcare ***:Culturally sensitive CL preventive action must be *** the scarce economic resources available for CL control in the country,the

关键词： Cutaneous leishmaniasis Control effort Qualitative evaluation Stakeholders'experiences Alta Verapaz Guatemala

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《冷山》的象征主义及其他

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电影文学 2005年第12期 62-63页

作者： freddy

听说电影《冷山》之后,才知道有小说。断断续续地读完,更增添了对电影的预期。而后终在银幕上看到了明格拉的影片。走出影院之后,有一个镜头却始终缠绕着我的思绪,不禁要对电影和小说作一番深入细致的比较。在黑暗的影院内,电影镜头开... 详细信息

听说电影《冷山》之后,才知道有小说。断断续续地读完,更增添了对电影的预期。而后终在银幕上看到了明格拉的影片。走出影院之后,有一个镜头却始终缠绕着我的思绪,不禁要对电影和小说作一番深入细致的比较。在黑暗的影院内,电影镜头开始慢慢慢慢地,然后一点一点地逐渐加速,围绕着一个垂死的士兵旋转。英曼开始咳嗽。

关键词：象征主义手法小说电影乌鸡深入细致影片士兵镜头观众莎拉

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Physicochemical Properties of Combustion Ashes of Some Trees(Urban Pruning)Present in the Neotropical Region

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Journal of Renewable Materials 2023年第10期11卷 3769-3787页

作者： John freddy Gelves-Díaz Ludovic Dorkis Richard Monroy-Sepúlveda Sandra Rozo-Rincón Yebrail Alexis Romero-Arcos Competitiveness and Sustainability for Development Research Group Faculty of EngineeringUniversidad Libre-Cúcuta SectionalCúcutaColombia Catalysis and Nanomaterials Research Group Faculty of MinesUniversidad Nacional de Colombia-Medellín SectionalMedellínColombia

Secondary lignocellulosic biomass has proved to be useful as an energy source through its oxidation by means of combustion *** accordance with the above,in this paper,we wanted to study the ash from urban pruning residues that are generated in cities in the *** such as Licania tomentosa,Azadirachta indica,Ficus benjamina,Terminalia catappa,Leucaena leucocephala,Prosopis juliflora and Pithecellobium dulce were selected because they have been previously studied and showed potential for thermal energy *** materials were calcined in an oxidizing atmosphere and characterized by X-ray diffraction and fluorescence,scanning electron microscopy with microchemistry,BET surface area,thermal gravimetric analysis,and differential scanning *** pH and apparent density were also *** results show high basicity materials(average pH 10),a behavior associated with the presence of chemical elements such as calcium,potassium,magnesium,chlorine,phosphorus,and ***,these materials have a very significant amorphous fraction(between 49%and 74.5%),the dominant crystalline phases are calcite,arcanite,sylvite,and *** ashes have low surface area and do not exceed 13 m^(2)/*** characteristic morphological aspects were observed in these ashes:a morphology of rounded grains where silicon content is highlighted,and lamellar morphologies where the presence of chlorine is ***,these ashes show four significant mass loss events(400℃,430℃,680℃,and 920℃),causing mass losses that vary between 25%and 40%.Through this study,it was possible to establish that,from a chemical point of view,these ashes are less dangerous in comparison with those of a mineral coal that was used as a ***,they require additional treatments for their disposal due to their high *** of their composition,these ashes have the potential to be used in the ceramic and cement industries,and in the manuf

关键词： Bottom ash chemical composition characterization mineralogy plant biomass pruning residues urban flora

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ELEMENTAL AND SPECIATION IMAGING OF ENVIRONMENTAL SAMPLES WITH SYNCHROTRON X-RAY ANALYSIS

ELEMENTAL AND SPECIATION IMAGING OF ENVIRONMENTAL SAMPLES WI...

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第十三届北京分析测试学术报告会暨展览会(BCEIA 2009)

作者： freddy ADAMS Micro-Trace Analysis Centre Department of ChemistryUniversity of Antwerp

Microscopic X-ray fluorescence analysis with synchrotron radiation sources(SRμ-XRF)is now well accepted as an important tool for microscopic/nanoscopic elemental analysis,complementing other beam methods of analysis ...

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Urban Plant Biomass Residues from the Neotropics and Their Potential for Thermal Energy Generation

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Journal of Renewable Materials 2023年第9期11卷 3547-3566页

作者： John freddy Gelves Díaz Ludovic Dorkis Richard Monroy-Sepúlveda Sandra Rozo-Rincón Gabriel de Jesús Camargo Vargas Competitiveness and Sustainability for Development Research Group Faculty of EngineeringUniversidad Libre-Cúcuta SectionalCúcutaColombia Catalysis and Nanomaterials Research Group Faculty of MinesUniversidad Nacional de Colombia-Medellín SectionalMedellínColombia Detecal Research Group Faculty of EngineeringUniversidad Libre-BogotáSectionalBogotáColombia

The material associated with tree pruning in a city in the Colombian neotropics was characterized in order to determine its energy *** species studied for their relevance in the territory were Prosopis juliflora,Licania tomentosa,Terminalia catappa,Azadirachta indica,Pithecellobium dulce,Ficus benjamina and Leucaena *** content,bulk density,hygroscopic response,elemental chemical analysis,proximal analysis,calorific value,ease of ignition and combustion,thermogravimetric and heat flow analysis,steam generation capacity,and qualitative analysis of gases(mass spectrometry)were *** results that were obtained show high initial moisture contents that vary between 37%and 67%and a variable density(when dry)between 0.21 and 0.41 g/***,it was shown that all residues have lower carbon and sulfur content compared to a reference mineral ***,the residues of some species have higher nitrogen contents compared to the same *** biomasses are characterized by their high content of volatile fractions and by having a lower content of inorganic matter compared to *** lower calorific values of these residues are between 14170 and 16928 kJ/kg,which are not negligible compared to other *** characteristic would be related to the high presence of hemicellulose in the *** gas monitoring revealed that there are different airflow needs in order to ensure complete *** generation tests showed that the calorific value should not be the most relevant criterion to establish the potential use of the waste,since leucaena leucocephala,despite having the highest calorific value,was the material with the lowest performance in steam generation.

关键词： Calorific value characterization combustion combustible material urban flora

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Helicobacter pylori HopE and HopV porins present scarce expression among clinical isolates

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World Journal of Gastroenterology 2010年第3期16卷 320-329页

作者： Maritza Lienlaf Juan Pablo Morales María Inés Díaz Rodrigo Díaz Elsa Bruce freddy Siegel Gloria León Paul R Harris Alejandro Venegas Microbial Pathogenesis and Vaccine Biotechnology Laboratory Department of Molecular Genetics and MicrobiologyFaculty of Biological SciencesPontificia Universidad Católica de Chile Gastroenterology Unit Faculty of MedicineUniversidad Austral de Chile Institute of Biochemistry Faculty of SciencesUniversidad Austral de Chile Department of Pediatrics School of MedicinePontificia Universidad Católica de Chile

AIM:To evaluate how widely Helicobacter pylori (H. pylori ) HopE and HopV porins are expressed among Chilean isolates and how seroprevalent they are among infected patients in ***: H. pylori hopE and hopV genes derived from strain CHCTX-1 were cloned by polymerase chain reaction (PCR), sequenced and expressed in Escherichia coli AD494 (DE3). Gel-purified porins were used to prepare polyclonal antibodies. The presence of both genes was tested by PCR in a collection of H. pylori clinical isolates and their expression was detected in lysates by immunoblotting. Immune responses against HopE, HopV and other H. pylori antigens in sera from infected and non-infected patients were tested by Western blotting using these sera as f irst antibody on recombinant H. pylori ***: PCR and Western blotting assays revealed that 60 and 82 out of 130 Chilean isolates carried hopE and hopV genes, respectively, but only 16 and 9, respectively, expressed these porins. IgG serum immunoreactivity evaluation of 69 H. pylori-infected patients revealed that HopE and HopV were infrequently recognized (8.7% and 10.1% respectively) compared to H. pylori VacA (68.1%) and CagA (59.5%) antigens. Similar values were detected for IgA serum immunoreactivity against HopE (11.6%) and HopV (10.5%) although lower values for VacA (42%) and CagA (17.4%) were obtained when compared to the IgG ***: A scarce expression of HopE and HopV among Chilean isolates was found, in agreement with the infrequent seroconversion against these antigens when tested in infected Chilean patients.

关键词： Helicobacter pylori Gene expression HopE and HopV porins Antigens Immune response

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A Grammatical Approach of Multi-Localization of GUIs: Application to the Multi-Localization of the Checkers Game GUI

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Journal of Software Engineering and Applications 2018年第11期11卷 552-567页

作者： Maurice Tchoupé Tchendji freddy-Viany Tatou Ahoukeng Department of Mathematics and Computer Science Faculty of Sciences University of Dschang Dschang Cameroon

By proposing tools that help for the accomplishment of tasks in almost all sectors of activities, computer science has revolutionized the world in a general way. Nowadays, it addresses the peculiarities of peoples through their culture in order to produce increasingly easy-to-use software for end users: This is the aim of software localization. Localizing a software consists among other things, in adapting its GUI according to the end user culture. We propose in this paper a generic approach allowing accomplishing this adaptation, even for multi-user applications like gaming applications, collaborative editors, etc. Techniques of functional interpretations of abstracts structures parameterized by algebras, constitute the formal base of our approach.

关键词： GUIs Context-Free Grammars Multi-Localization Abstract Syntax Tree and Its Interpretations XML GUI Description Language Haskell

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Early GCase activity is a predictor of long-term cognitive decline in Parkinson’s disease

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Translational Neurodegeneration 2023年第1期12卷 329-332页

作者： Linn Oftedal Johannes Lange Kenn freddy Pedersen Aleksander Hagen Erga Ingvild Dalen Ole-Bjørn Tysnes Guido Alves Jodi Maple-Grødem Centre for Movement Disorders Centre for Brain HealthStavanger University Hospital4011 StavangerNorway Department of Chemistry Bioscience and Environmental EngineeringUniversity of Stavanger4021 StavangerNorway Department of Neurology Stavanger University Hospital4011 StavangerNorway Department of Social Studies University of Stavanger4021 StavangerNorway Section of Biostatistics Department of ResearchStavanger University Hospital4011 StavangerNorway Department of Clinical Medicine University of Bergen5021 BergenNorway Department of Neurology Haukeland University Hospital5053 BergenNorway

Dementia is a serious complication for many patients with advanced Parkinson’s disease(PD)and its incidence is increased in PD patients harbouring a mutation in GBA1[1].However,cognitive impairment is not limited to late disease stages and can also manifest in patients with early untreated disease,leading to diminished social function and increased disability and caregiver *** have previously shown that reduced activity of the GBA1 protein product,the lysosomal enzyme glucocer-ebrosidase(GCase),is linked with long-term progres-sion to PD dementia[2].In this study,we extend this by determining the association of cerebrospinal fluid(CSF)GCase activity with the development of cognitive impair-ment through the analysis of neuropsychological test data collected over 10 years of prospective follow-up of patients with incident PD.

关键词： impairment progr cognitive

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