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Customized molecular tools to strengthen metabolic engineering of cyanobacteria

Green Carbon 2024年第2期2卷 149-163页

作者： Stephan Klähn franz opel Wolfgang R.Hess Department of Solar Materials Biotechnology Helmholtz Centre for Environmental Research–UFZLeipzig 04318Germany Genetics and Experimental Bioinformatics Albert-Ludwig University FreiburgFreiburg im Breisgau 79104Germany

Cyanobacteria are promising oxygenic phototrophs for the production of various *** their(photo)biotechnological exploitation,molecular tools are required,such as,for the introduction and expression of heterologous genes,or the modulation of enzyme activities or entire *** and strategies for the development of photosynthetic biomanufacturing technologies based on cyanobacteria have been extensively reviewed,as well as certain specialized aspects of their genetic ***,options for metabolic engineering of specific cyanobacterial cells are still less developed than those for other bacteria of biotechnological *** addition to the standard genetic toolbox for“classical”metabolic engineering,we emphasize certain aspects,including recently developed vector systems for the extrachromosomal maintenance of genes and approaches based on clustered regularly interspaced short palindromic repeats(CRISPR)*** highlight the development of custom molecular tools for specific strains or products,discuss the emerging use of small regulatory proteins that appear promising for advanced metabolic engineering approaches to promote specific product formation,and provide an overview of suitable online ***,we discuss the current trends in this field and indicate their potential,such as using suitable product sensors that enable systematic screening,and optimization approaches.

关键词： Metabolic engineering Cyanobacteria Synthetic plasmids Regulatory proteins Sensors

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Lateral Intra-Articular Transposition of the Anterior Tibialis Tendon for the Treatment of Relapsed Clubfoot in Toddlers: A Previously Unreported Surgical Technique

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Open Journal of Orthopedics 2014年第3期4卷 53-59页

作者： Dayton opel Samuel Abrams Matthew Halanski Kenneth Noonan Department of Orthopedics and Rehabilitation University of Wisconsin Madison USA University of Wisconsin School of Medicine and Public Health Madison USA

Dynamic supination of the foot is a common residual deformity in children with clubfeet treated with the Ponseti method. Transfer of the anterior tibialis tendon (ATT) to the lateral cuneiform is an effective method for correcting this deformity when the cuneiform is ossified in children who are 3 to 5 years of age. We describe two cases of a previously unreported method of ATT transposition for correction of bilateral residual dynamic supination in a 26-month-old and a 19-month-old patient. Both patients presented shortly after birth with bilateral congenital idiopathic clubfoot and were initially treated with the Ponseti method. Both had residual deformity following initial treatment that included posterior contracture and metatarsus adductus with dynamic forefoot supination. This was surgically corrected with a posterior release and medial release of the 1st metatarsal/1st cuneiform joint. To correct dynamic supination, the ATT was transplanted laterally into the released midfoot joint. These two patients were followed post-operatively for 7.5 years and have correction of their residual deformity in both feet based on subjective functioning, appearance, range of motion, strength, and gait. Both have excellent lateral pull of their ATT, which functions as a strong foot dorsiflexor. No residual supination is present. This is the first report of lateral transposition of the ATT as an interposition graft at the released 1st metatarsal/1st cuneiform joint in patients with relapsed clubfoot. We suggest that this method should provide a high level of functioning in children with relapsed supination deformity and whose 3rd cuneiform has not yet ossified.