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Cellular Forms in Cultivation in Suspension of Bougainvillea glabra Choisy Variety Surprise

Journal of Agricultural Science and Technology(A) 2018年第4期8卷 203-211页

作者： Christian Marely Rodriguez-Salazar José Roman-Reynosa Sandra Victoria Avila-Reyes franklin loring-younce Antonio Ruperto Jiménez-Aparicio Silvia Evangelista-Lozano Centro de Desarrollo de Productos Bióticos del Instituto Politécnico Nacional (CEPROBI-IPN) Carretera Yautepec-JojutlaKm 6calle CEPROBI no. 8Colonia San IsidroC.P. 62731YautepecMorelosMéxico Tecnológico de Zacatepec del Instituto Nacional de México Calzada Tecnológico 27Col. Plan de AyalaC.P. 62780Zacatepec de HidalgoMorelosMéxico CONACyT-Instituto Politécnico Nacional (CEPROBI-IPN) Carretera Yautepec-JojutlaKm 6calle CEPROBI No. 8Colonia San IsidroC.P. 62731YautepecMorelosMéxico School of Food Science Washington State UniversityPullmanWA 99164-6376USA

The objective of this work was to describe the predominant cell forms in the phases of growth kinetics in a suspension culture of the Bougainvillea glabra Choisy variety *** in suspension with Murashige and Skoog(MS)basal culture medium were supplemented with six different concentrations and combinations of vegetable growth regulator(VGR)of type auxin(2,4-D and NAA)and cytokinin(BAP)(0.2-5.3 mg/L)and a *** callus was obtained from leaf explants ex vitro to in vitro culture in solid MS medium using PhytagelTM as gelling agent to 2.2 g/L.A portion of callus(1.0 g)was used as cellular inoculum and grown under controlled conditions(50 mL,120 rpm,25°C and luminous intensity of 48μmol m2/s).The best treatment with significant differences(p≤0.05)were with a hormonal relation of 2:1 of BAP and NAA,respectively,with a fresh weight yield ranging from 1.7905 g to 5.8340 g,which represents around 70%.An adaptation phase was observed from day 0 to day 14 on the curve of fresh weight;an exponential phase at day 14 to day 19 and a declination phase at day *** forms in the adaptation phase were elongated cells,a few globular and a few with kidney *** the exponential phase,these cells formed small aggregates of globular *** the death phase,brown,elongated,damaged and fragmented cells were *** this data obtained it is possible to establish the subculture time in fresh medium.

关键词： Bougainvillea kinetics of cell growth in vitro culture

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