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Experimental validation of inter-subspecific genetic diversity in rice represented by th. differences between th. DNA sequences of 'Nipponbare' and '93-11'

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Ch.nese Science Bulletin 2007年第10期52卷 1327-1337页

作者： MEI h.nWei FENG FangJun LU BaoRong WEN WeiWei Andrew h. PATERSON CAI XingXing Ch.N Liang frank A. FELTUS XU XiaoYan WU Jingh.ng YU XinQiao Ch.N h.ngWei LI Ying LUO LiJun Sh.ngh.i Agrobiological Gene Center Shanghai 201106 China Institute of Biodiversity Science Fudan University Shanghai 200433 China h.azh.ng Agricultural University Wuhan 430070 China Plant Genome Mapping Laboratory University of Georgia Athens GA30602 USA

Th. pedigrees of th.ee sequenced rice cultivars were analyzed to sh.w th.t a majority of th. genetic composition of 'Nipponbare' originates from japonica cultivars wh.le th. minority originates from indica cultivars. In contrast, '93-11' is derived mainly from indica cultivars with.a smaller contribution from japonica cultivars. All ancestors of 'Guang lu ai 4' appeared to be indica lines. A set of molecular markers (46 InDels and 53 SSRs) polymorph.c between 'Nipponbare' and '93-11' were examined in 46 typical indica and 47 typical japonica cultivars selected from 443 accessions according to Ch.ng's index. All cultivars were divided into indica and japonica groups with.ut overlapping wh.n clustered by Ch.ng's index, InDels and SSRs. Much.h.gh.r InDel and SSR diversity between groups th.n with.n groups implies th.t th. marker polymorph.sms between 'Nipponbare' and '93-11' represent a large proportion of inter-subspecific diversity. About 85% of indica cultivars and more th.n 90% of japonica cultivars were confirmed to h.ve th. same PCR banding patterns as '93-11' and 'Nipponbare', respectively. Some polymorph.c loci between 'Nipponbare' and '93-11' cannot be validated in oth.r indica and japonica cultivars, eith.r as subspecies-specific but not predominant alleles, or alleles not specific between th. two groups. It was concluded th.t molecular markers developed from sequence polymorph.sm between 'Nipponbare' and '93-11' often represent inter-subspecific diversity, alth.ugh.some exceptions were sensitive to eith.r particular marker loci or particular cultivars.

关键词： 93-11水稻日本晴稻亚种间遗传多态性 DNA序列实验验证

Model for end-stage liver disease score versus Ch.ld score in predicting th. outcome of surgical procedures in patients with.cirrh.sis

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World Journal of Gastroenterology 2008年第11期14卷 1774-1780页

作者： Maarouf A h.teit Amaar h.Gh.zale Andrew J Bain Eli S Rosenberg Kirk A Easley frank A Anania Robin E Ruth.rford Division of Digestive Diseases Emory UniversityAtlanta GeorgiaUnited States Department of Internal Medicine Emory UniversityAtlantaGeorgiaUnited States Department of Biostatistics-Rollins Sch.ol of Public h.alth Emory UniversityAtlanta GeorgiaUnited States

AIM： To determine factors affecting th. outcome of patients with.cirrh.sis undergoing surgery and to compare th. capacities of th. Ch.ld-Turcotte-Pugh.（CTP） and model for end-stage liver disease （MELD） score to predict th.t outcome. METh.DS： We reviewed th. ch.rts of 195 patients with.cirrh.sis wh. underwent surgery at two teach.ng h.spitals over a five-year period. Th. combined endpoint of death.or h.patic decompensation was considered to be th. primary endpoint. RESULTS： Patients wh. reach.d th. endpoint h.d a h.gh.r MELD score, a h.gh.r CTP score and were more likely to h.ve undergone an urgent procedure. Among patients undergoing elective surgical procedures, no statistically significant difference was noted in th. mean MELD （12.8 ＋ 3.9 vs 12.6 ＋ 4.7, P = 0.9） or in th. mean CTP （7.6 ± 1.2 vs 7.7 ± 1.7, P = 0.8） between patients wh. reach.d th. endpoint and th.se wh. did not. Both.mean scores were h.gh.r in th. patients reach.ng th. endpoint in th. case of urgent procedures （MELD： 22.4 ± 8.7 vs 15.2 ± 6.4, P = 0.0007; CTP： 9.9 ± 1.8 vs 8.5 ± 1.8, P = 0.008）. Th. performances of th. MELD and CTP scores in predicting th. outcome of urgent surgery were only fair, with.ut a significant difference between th.m （AUC = 0.755 ± 0.066 for MELD vs AUC = 0.696 ± 0.070 for CTP, P = 0.3）. CONCLUSION： Th. CTP and MELD scores performed equally, but only fairly in predicting th. outcome of urgent surgical procedures. Larger studies are needed to better define th. factors capable of predicting th. outcome of elective surgical procedures in patients with.***, but only fairly in predicting th. outcome of urgent surgical procedures. Larger studies are needed to better define th. factors capable of predicting th. outcome of elective surgical procedures in patients with.cirrh.sis.

关键词： Liver cirrh.sis Prognosis Severity of illness index Surgical procedures Operative Postoperative complications

Analogies between Density Functional Th.ory Response Kernels and Derivatives of Th.rmodynamic State Functions

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物理化学学报 2018年第6期34卷 699-707页

作者： GEERLINGS Paul DE PROFT frank FIAS Stijn General Ch.mistry (ALGC). Free University Brussels (VUB) Pleinlaan 2. 1050 Brussel. Belgium.

In view of its use as reactivity th.ory,Conceptual Density Functional Th.ory(DFT),introduced by Parr et al.,h.s mainly concentrated up to now on th. E = E[N,v] ***,different ensemble representations can be used involving oth.r variables also,such.as ρ and μ.In th.s study,th.se different ensemble representations(E,?,F,and R) are briefly *** attention is th.n given to th. corresponding second-order(functional) derivatives,and th.ir analogieswith.th. second-order derivatives of th.rmodynamic state functions U,F,h.and G,wh.ch.are related to each.oth.r via Legendre transformations,just as th. DFT functionals(Nalewajski and Parr,1982).Starting from an analysis of th. convexity/concavity of th. DFT functionals,for wh.ch.explicit proofs are discussed for some cases,th. positive/negative definiteness of th. associated kernels is derived and a detailed comparison is made with.th. th.rmodynamic *** stability conditions in th.rmodynamics are similar in structure to th. convexity/concavity conditions for th. DFT ***,th. DFT functionals are scrutinized based on th. convexity/concavity of th.ir two variables,to yield th. possibility of establish.ng a relationsh.p between th. th.ee second-order reactivity descriptors derived from th. considered *** two ensemble representations,F and ?,F is eliminated as it h.s two dependent(extensive)variables,N and ρ.For ?,on th. oth.r h.nd,wh.ch.is concave for both.of its intensive variables(μ and υ),an inequality is derived from its th.ee second-order(functional) derivatives:th. global softness,th. local softness,and th. softness *** with.th. negative value of th. diagonal element of th. linear response function,th.s inequality is sh.wn to be compatible with.th. Berkowitz-Parr relationsh.p,wh.ch.relates th. functional derivatives of ρ with.υ,at constant N and μ.Th.s was recently at stake upon quantifying Koh.'s Nearsigh.edness of Electronic *** analogy

关键词： Conceptual DFT Response kernels Analogy with lh.rmodynamics Stability-concavity/convexity

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基于便携式坐标测量机的大齿轮测量方法

光学精密工程 2013年第7期21卷 1763-1770页

作者：林虎 frank h.rtig Karin Kniel 石照耀北京工业大学机械工程与应用电子技术学院 Ph.sikalisch.Tech.isch. Bundesanstalt Braunsch.eig und Berlin 38116 BraunschweigGermany

针对目前便携式坐标测量机测量大齿轮的采样策略和评定算法存在的不足,设计了两种特殊的辅助标尺-渐开线标尺及螺旋线标尺。利用设计的两种标尺在大齿轮齿面上对齿廓及螺旋线的测量点进行标注,开展了齿轮的在位重复性测量的实验研究。... 详细信息

针对目前便携式坐标测量机测量大齿轮的采样策略和评定算法存在的不足,设计了两种特殊的辅助标尺-渐开线标尺及螺旋线标尺。利用设计的两种标尺在大齿轮齿面上对齿廓及螺旋线的测量点进行标注,开展了齿轮的在位重复性测量的实验研究。利用关节臂坐标测量机及激光跟踪仪测量系统在德国计量研究院研制的1m外径齿轮样板上开展了测量实验,采用INVOLUTE Pro对采样数据点进行评定,给出了测量结果及测量不确定度。测量实验表明,基于新的齿轮测量方法,关节臂坐标测量机比激光跟踪仪测量系统测量结果更为精确,测试结果与标定值最大相差8.16μm。实验结果验证了提出方法的有效性,为便携式坐标测量机在大齿轮测量领域的应用提供了依据。

关键词：大齿轮测量便携式坐标测量机大齿轮样板

Video Traces for Network Performance Evaluation 2007

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解剖学部位对正常口腔粘膜上皮细胞及其分化的影响:形态定量学研究

2007年

作者： Patrick Seeling frank h. P. Fitzek Martin Reisslein

来源：畅想之星图书光盘版（南... 评论

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实用口腔医学杂志 1992年第3期8卷 179-181页

作者：金岩杨连甲 frank h. Wh.te CC Tam 第四军医大学口腔医学院香港大学医学院解剖学系

选用VIDAS图象分析系统对正常颊部、硬腭和软腭的粘膜上皮细胞的形态学指标进行测量分析。提供了多项形态学指标的正常数值,与此同时还发现:(1)硬腭粘膜上皮细胞数量最多但上皮厚度最薄;(2)在细胞分化过程中细胞的胞体和胞核均明显增大... 详细信息

选用VIDAS图象分析系统对正常颊部、硬腭和软腭的粘膜上皮细胞的形态学指标进行测量分析。提供了多项形态学指标的正常数值,与此同时还发现:(1)硬腭粘膜上皮细胞数量最多但上皮厚度最薄;(2)在细胞分化过程中细胞的胞体和胞核均明显增大,尤以胞体增大最为明显;(3)硬腭粘膜上皮细胞和胞核无论在基底层还是棘层数值都最小;(4)BAL/BL比值可以有效的反映上皮钉突的状况。

关键词：形态定量学颊粘膜口腔粘膜

Cardiac beta-defensins upregulate with.a h.gh.fat diet and influence monocyte migration

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Open Journal of Internal Medicine 2013年第2期3卷 81-94页

作者： Annika Linde Ch.istoph.r Ross frank Blech. Gerald h. Lush.ngton Tonatiuh.Melgarejo Center for Veterinary h.alth.Sciences Oklahoma State University Stillwater USA Department of Anatomy & Ph.siology Kansas State University Manhattan USA Department of Medicinal Ch.mistry University of Kansas Lawrence USA

Inflammation plays a central role in development of cardiovascular path.logy, and enh.nced understanding of th. innate immune response will h.lp direct novel th.rapeutic strategies to address h.art disease. h.st defense peptides (h.Ps)—h.reunder defensins—exh.bit antimicrobial, ch.motactic, tissue h.aling and oth.r key biological properties. Beta-defensin expression in wh.le-h.art-h.mogenate h.s been reported in different species, and plasma alph.-defensins h.ve been associated with.cardiovascular morbidity and mortality. Still, th. role of defensins in cardiac path.ph.siology remains widely undetermined. h.re, we sh.w th.t a subset of rat-beta-defensins (rBDs) is constitutively expressed in th. myocardium, and th.t th.ir gene-expression level is influenced by systemic exposure to inflammatory mediators (h.gh.at-diet and lipopolysacch.ride). Using synth.tic analogues of select rBD peptides, we evaluated th. antimicrobial activity of th.se h.Ps against clinically relevant path.gens and th.ir ability as immunoregulatory compounds. We found th.t an innate myocardial response th.t involves rBDs is activated by h.gh.at-diet feeding in rats, and th.t th.se h.Ps influence monocyte migration-findings th.t suggest th. peptides responding to exogenous danger-signals, and act with.n th. context of a myocardial “first-line-of-defense”.

关键词： Antimicrobial Peptides Myocardial Endotoxin Dietary Lipid Cell Migration

判断硬腭粘膜癌前病变的新方法:组织形态定量相关分布图分析法

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实用口腔医学杂志 1992年第3期8卷 191-192页

作者：金岩 frank h. Wh.te 杨连甲 CC Tam 第四军医大学口腔医学院香港大学医学院解剖学系

用组织形态定量学方法对硬腭部有高度恶变可能的癌旁上皮基底细胞的N/C比值和胞核体积进行测量,同时选取相同部位的良恶性病变作为对照;在此基础上用N/C比值和胞核体积两项指标进行相关分布图观察和分析,结果显示这一方法不仅可以显示... 详细信息

用组织形态定量学方法对硬腭部有高度恶变可能的癌旁上皮基底细胞的N/C比值和胞核体积进行测量,同时选取相同部位的良恶性病变作为对照;在此基础上用N/C比值和胞核体积两项指标进行相关分布图观察和分析,结果显示这一方法不仅可以显示病变的不同状态、病变的程度和病变之间相互关系,而且可以反映同一病变组内个体病变的病变程度。

关键词：形态定量学硬腭癌旁上皮炎症

病理状态对颊粘膜上皮细胞分化的影响:形态定量学分析

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实用口腔医学杂志 1992年第3期8卷 188-190页

作者： frank h. Wh.te 金岩杨连甲 CC Tam 香港大学医学院解剖学系第四军医大学口腔医学院

用形态定量学方法并结合病理改变值(PAR)对颊粘膜在正常、炎症、过度角化、白斑、鳞癌及癌旁上皮7种状态下的上皮细胞分化的情况进行了观察分析。结果显示白斑、癌旁上皮及鳞癌组在分化中胞核的增大最为明显,与此同时白斑的细胞数量也... 详细信息

用形态定量学方法并结合病理改变值(PAR)对颊粘膜在正常、炎症、过度角化、白斑、鳞癌及癌旁上皮7种状态下的上皮细胞分化的情况进行了观察分析。结果显示白斑、癌旁上皮及鳞癌组在分化中胞核的增大最为明显,与此同时白斑的细胞数量也明显增多。

关键词：形态定量学颊粘膜白斑细胞分化