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Spectro-Microscopy of Living Plant Cells

Molecular Plant 2012年第1期5卷 14-26页

作者： Klaus h.tter Alfred J. Meixner frank Sch.eifenbaum Center for Plant Molecular Biology （ZMBP） Plant Physiology and Biophysical Chemistry University of Tubingen Auf der Morgenstelle 1 72076 Tuebingen Germany Institute for Ph.sical and Th.oretical Ch.mistry （IPTC） Nano-Optics University of Tubingen Auf der Morgenstelle 8 72076 Tubingen Germany

Spectro-microscopy, a combination of fluorescence microscopy with.spatially resolved spectroscopic tech.i- ques, provides new and exciting tools for functional cell biology in living organisms. Th.s review focuses on recent devel- opments in spectro-microscopic applications for th. investigation of living plant cells in th.ir native tissue context. Th. application of spectro-microscopic meth.ds led to th. recent discovery of a fast signal response path.ay for th. brassi- nosteroide receptor BRI1 in th. plasma membrane of living plant cells. Moreover, th. competence of different plant cell types to respond to environmental or endogenous stimuli was determined in vivo by correlation analysis of different optical and spectroscopic readouts such.as fluorescence lifetime （FLT）. Furth.rmore, a new spectro-microscopic tech.ique, fluorescence intensity decay sh.pe analysis microscopy （FIDSAM）, h.s been developed. FIDSAM is capable of imaging low- expressed fluoroph.re-tagged proteins at h.gh.spatial resolution and precludes th. misinterpretation of autofluorescence artifacts. In addition, FIDSAM provides a very effective and sensitive tool on th. basis of F6rster resonance energy transfer （FRET） for th. qualitative and quantitative determination of protein-protein interaction. Finally, we report on th. quan- titative analysis of th. ph.tosystem I and II （PSI/PSII） ratio in th. ch.oroplasts of living Arabidopsis plants at room tem- perature, using h.gh.resolution, spatially resolved fluorescence spectroscopy. With.th.s tech.ique, it was not only possible to measure PSI/PSII ratios, but also to demonstrate th. differential competence of wild-type and carboh.drate-deficient plants to adapt th. PSI/PSII ratio to different ligh. conditions. In summary, th. information content of standard microscopic images is extended by several dimensions by th. use of spectro-microscopic approach.s. Th.refore, novel cell ph.siolog- ical and molecular topics can be addressed and valuable insigh

关键词： Fluorescence microscopy FLIM FRET ph.tosystems protein-protein interaction FIDSAM.

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Selective Suspension in Aqueous Sodium Dodecyl Sulfate According to Electronic Structure Type Allows Simple Separation of Metallic from Semiconducting Single-Walled Carbon Nanotubes

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Nano Research 2009年第8期2卷 599-606页

作者： Kai Mosh.mmer frank h.nnrich.Manfred M.Kappes Forsch.ngszentrum Karlsruh. Institut für NanotechnologieD-76021 KarlsruheGermany Institut für Ph.sikalisch. Ch.mie Universität KarlsruheD-76128 KarlsruheGermany

Both.density gradient centrifugation and gel electroph.resis h.ve been reported to allow h.gh.th.ough.ut separation of metallic from semiconducting single-walled carbon nanotubes(SWNTs)wh.n using aqueous sodium dodecyl sulph.te(SDS)*** sh.w h.re th.t both.meth.ds rely on an initial dispersion-by-sonication step,wh.ch.is already selective with.respect to electronic structure *** corresponding aqueous SDS“starting”suspensions obtained after sonication and purifi cation by simple centrifugation(70,000 g,1 h.contain semiconducting SWNTs primarily in th. form of small bundles wh.reas metallic SWNTs are predominantly suspended as individual *** gradient centrifugation th.n separates th. bundles from th. individual tubes on th. basis of differences in th.ir overall buoyant *** electroph.resis separates th. longer bundles from th. sh.rter individual tubes on th. basis of th.ir different *** also demonstrate th.t such.starting suspensions can be fractionated according to electronic structure type by even simpler tech.iques such.as size exclusion ch.omatograph. or gel fi ltration,th.s opening th. way for simple scale-up.

关键词： Single-walled carbon nanotubes metallic–semiconducting separation electroph.resis density gradient centrifugation size exclusion ch.omatograph.

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牛奶可乐经济学

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作者： (美)罗伯特·弗兰克(Robert h. frank)

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用于焙烤面包及涂层混合料的膨松剂

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中国食品工业 2001年第5期 18-19页

作者： frank h. Ch.ng Rh.dia有限公司

众所周知，膨松剂在焙烤中的主要作用是在焙烤或混合生面团和稀面糊时提供所需气体，以使焙烤产品获得充气膨松的效果，增加体积并改善口感及外观质量。

关键词：焙烤膨松剂烤面包面团口感外观质量涂层主要作用效果增加

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Precise role of h.pylori in duodenal ulceration

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World Journal of Gastroenterology 2006年第40期12卷 6413-6419页

作者： Mich.el h.bsley frank I Tovey Joh. h.lton Department of Surgery Royal Free and University College Medical School London United Kingdom Department of Microbiology Royal Free and University College Medical School London United Kingdom

Th. facts th.t h.pylori infection is commoner in duodenal ulcer (DU) patients th.n in th. normal population, and th.t eradication results in most cases being cured, h.ve led to th. belief th.t it causes DU. h.wever, early cases of DU are less likely th.n establish.d ones to be infected. h.pylori-negative cases are usually ascribed to specific associated factors such.as non-steroidal anti-inflammatory drugs (NSAIDs), Croh.’s disease, and h.pergastrinaemia, but even after excluding th.se, several h.pylori-negative cases remain and are particularly common in areas of low prevalence of h.pylori infection. Moreover, th.s incidence of h.pylori negative DU is not associated with.a fall in overall DU prevalence wh.n compared with.countries with.a h.gh.r h.pylori prevalence. In countries with.a h.gh.h.pylori prevalence th.re are regional differences in DU prevalence, but no evidence of an overall h.gh.r prevalence of DU th.n in countries with.a low h.pylori prevalence. Th.re is no evidence th.t virulence factors are predictive of clinical outcome. After h.aling following eradication of h.pylori infection DU can still recur. Medical or surgical measures to reduce acid output can lead to long-term h.aling despite persistence of h.pylori infection. Up to h.lf of cases of acute DU perforation are h.pylori negative. Th.se findings lead to th. conclusion th.t h.pylori infection does not itself cause DU, but leads to resistance to h.aling, i.e., ch.onicity. Th.s conclusion is sh.wn not to be incompatible with.th. universally h.gh.prevalence of DU compared with.controls.

关键词： Duodenal ulceration h.pylori infection Not causal Delays h.aling

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用形态定量学方法研究颊粘膜上皮棘层细胞在良、恶性病变中的变化

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实用口腔医学杂志 1992年第3期8卷 181-183页

作者： frank h. Wh.te 金岩 Daniel CC Tam 杨连甲郑秉源香港大学医学院解剖学系第四军医大学口腔医学院病理科

对口腔颊部粘膜在正常、炎症、纤维增生、乳头状瘤、癌旁上皮及鳞癌癌巢的状态下的上皮棘层细胞及胞核的面积、体积和胞核的直径、形态进行了检测分析,结果提示反映细胞和胞核大小的形态学指标对于判断病变的程度有着重要的意义。

关键词：形态定量学细胞细胞核颊粘膜

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Direct ex vivo analysis of dendritic cells in patients with.h.patocellular carcinoma

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World Journal of Gastroenterology 2006年第20期12卷 3275-3282页

作者： Lars A Ormandy Anatol Frber Tobias Cantz Susanne Petrykowska h.iner Wedemeyer Monique h.rning frank Leh.er Mich.el P Manns Firouzeh.Korangy Tim F Greten Department of Gastroenterology h.patology and Endocrinology Hannover Medical SchoolGermany Departments of Visceral and Transplantation Surgery h.nnover Medical Sch.ol Germany

AIM： To analyze th. ph.notype and function of dendritic cells （DC） from patients with.h.patocellular carcinoma （h.C） in order to understand th.ir role in th.s disease. METh.DS： Myeloid dendritic cells were enumerated in periph.ral blood of h.C patients. CD80, CD83, CD86 and h.A-DR expression on naive and stimulated myeloid dendritic cells from periph.ral blood were analyzed. Myeloid dendritic cells were isolated from periph.ral blood and th.ir function was tested. Ph.gocytosis was analyzed using FITC-dextran beads, peptide specific stimulation, th. capacity to stimulate allogeneic T cells and secretion of cytokines upon poly dZ：dC was tested. RESULTS： Myeloid dendritic cells were reduced in patients with.h.C. No differences in CD80, CD83, CD86 and h.A-DR expression were found on naive and stimulated myeloid dendritic cells from h.C patients and h.alth. controls. Normal ph.gocytosis or stimulation of peptide specific T cells was observed in contrast to an impaired allo-stimulatory capacity and a reduced IL-12 secretion. CONCLUSION： Impaired IL-12 production of mDCs in patients could lead to an impaired stimulatory capacity of naive T cells suggesting th.t IL-12 directed th.rapies may enh.nce tumor specific immune responses in h.C patients.

关键词： Myeloid DC h.patocellular carcinoma CD80 CD83 CD86

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A collagen plug with.sh.pe memory to seal iatrogenic fetal membrane defects after fetoscopic surgery

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生物活性材料(英文) 2023年第2期20卷 463-471页

作者： Rob T.C.Meuwese Elly M.M.Versteeg Joris van Drongelen Daniëlle de h.og Debora Bouwh.is frank P.h.A.Vandenbussch. Toin h.van Kuppevelt Willeke F.Daamen Department of Bioch.mistry Radboud Institute for Molecular Life SciencesRadboud University Medical CenterPO Box 91016500 HBNijmegenthe Netherlands Department of Obstetrics&Gynecology Radboud University Medical CenterPO Box 91016500 HBNijmegenthe Netherlands Department of Obstetrics&Gynecology Helios Klinikum DuisburgAn der Abtei 747166DuisburgGermany

Iatrogenic preterm premature rupture of fetal membranes(iPPROM)after fetal surgery remains a strong trigger for premature *** fetal membrane defects do not h.al spontaneously and amniotic fluid leakage and ch.rioamniotic membrane separation may occur,we developed a biocompatible,fetoscopically-applicable collagen plug with.sh.pe memory to prevent *** plug expands directly upon employment and seals fetal membranes,h.nce preventing amniotic fluid leakage and potentially *** typeⅠcollagen plugs were given sh.pe memory and crimped to fit th.ough.a fetoscopic cannula(Ø3 mm).Expansion of th. plug was examined in ph.sph.te buffered saline(PBS).Its sealing capacity was studied ex vivo using h.man fetal membranes,and in situ in a porcine bladder *** crimped plug with.sh.pe memory expanded and tripled in diameter with.n 1 min wh.n placed into PBS,wh.reas a crimped plug with.ut sh.pe memory did *** both.h.man fetal membranes and porcine bladder,th. plug expanded in th. defect,secured itself and sealed th. defect with.ut membrane *** conclusion,collagen plugs with.sh.pe memory are promising as medical device for rapid sealing of fetoscopic defects in fetal membranes at th. endoscopic entry point.

关键词： Biomaterial Crosslinking Fetoscopy Fetal th.rapy Medical device Regenerative medicine

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牛奶可乐经济学

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作者： (美)罗伯特·弗兰克(Robert h. frank)

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Burn Injuries in Elderly—A Retrospective Analysis of a Ten Year Period

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Modern Plastic Surgery 2012年第2期2卷 13-19页

作者： frank Siemers Eirini Liodaki Karl L. Mauss Felix h. Stang Tobias von Wild Peter Mailaender Plastic Surgery University Clinic Schleswig-Holstein Lubeck Germany

Objective: Geriatric patients represent a separate group in th. treatment of burn injury regarding path.genesis, path.ph.siology and th.rapeutic procedure. Meth.ds: From 2000 to 2010 on our burn unit 53 patients aged over 60 years were treated. Th. mean age of th. 27 women and 26 men was 76 years (60 - 102 years), with.an average of 33.2% of total burn surface area. 25 patients (47.2%) h.d severe inh.lation injury, 77.4% of th. patients suffered full th.ckness burn injuries. Mean ABSI Score was 12. Relevant additional diseases were coronary h.art disease, h.art insufficiency, h.pertension, COPD, adiposity and ch.onic alcoh.lism. Results: 22 patients were treated by early operative intervention (day 0 - 4), 13 of th.se patients survived furth.r process. In 17 cases first operative procedure could take place only at a later date, 14 patients deceased before operation. 26 patients developed pneumonia. Th. total morbidity amounted to 26 of 53 patients (49%). A total of 19 injured with.a burn index over 80 survived. 9 of th.m h.d an index of more th.n 100. Usual indices as th. “Zellweger Index” and th. ABSI-Score are not adequate to determine th. prognosis of th.rmal injuries in th. elderly. Conclusion: Th. treatment of elderly with.burn trauma is affected by many factors. Compared to younger patients th. increased incidence of an inh.lation injury is decisive for th. prognosis of older patients. Th. frequency of pre-existing diseases is clearly largely th.n with.younger burn injured patients. Th.se prognostically relevant parameters were not sufficiently considered by establish.d score systems.

关键词： Burn Injury Intensive Care Elderly

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