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Infectious Diseases of Poverty 2017年第1期6卷 1185-1195页

作者： Mellina YAMAMURA Marcelino santos NETO francisco CHIARAVALLOTI NETO Luiz Henrique ARROYO Antonio Carlos Vieira RAMOS Ana Angelica Rego de QUEIROZ Aylana de souza BELCHIOR Danielle Talita dos santos Juliane de Almeida CRISPIM Ione Carvalho PINTO Severina Alice da Costa UCHOA Regina Celia FIORATI Ricardo Alexandre ARCENCIO School of Nursing of the University of Sao Paulo Ribeirao Preto Campus(EERP/USP)Ribeirao retoSPBrazil Federal University of Maranhao(UFMA) Sao LuísMABrazil School of Public Health of the University of Sao Paulo(FSP/USP) Sao PauloSPBrazil Federal University of Rio Grande do Norte(UFRN) NatalRNBrazil School of Medicine of the University of Sao Paulo Ribeirao Preto Campus(FMRP/USP)Ribeirao PretoSPBrazil

Background:In Brazil,people still fall ill and die from tuberculosis(TB),and this can be explained by the significant impasse in the equity of distribution of therapeutic resources to the population as a *** aim was to identify geographical areas which have shown progress in terms of equity(of income,schooling and urban occupancy)and test its effect on mortality from TB in a municipality of southeast ***:It is an ecological study considering TB as the basic cause for deaths registered between 2006 and 2013 on the Mortality Information System and other variables obtained through the demographic Census of the Brazilian Institute of Geography and Statistics(2010).The geographical area for analysis comprised the areas of coverage of the health *** indicators have been constructed through the Principal Component Analysis(PCA).The cases were geocoded and the annual mortality rate from TB was calculated with smoothing using the local empirical Bayesian *** linear regression was then *** was confirmation of the existence of spatial dependence of residue through the application of the Global Moran I test,and application of the Models with Global Spatial Effects,to identify the best standard of spatial ***:The mortality rates ranged from 0.00 to 2.8 deaths per 100,000 people,per *** the PCA,three indicators were constructed,and designated as indicators of income,social inequality,and social *** multiple linear regression,the indicator of social equity was statistically significant(Pdependence(Moran I=0.21,P=0.003455).The best model to deal with existing spatial dependence was the Spatial Lag ***:The better social conditions have shown progress in reducing mortality from TB,thereby reinforcing the achievement of Sustainable development *** addition,cartography was also applied,which can be replicated

关键词： Tuberculosis Spatial analysis Mortality Cause of death

Small river basin and estuarine sediment fluxes:The magnitude necessary for coastal dispersion and siltation effects on a coral reef

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International Journal of Sediment Research 2019年第6期34卷 591-599页

作者： Paula Sagili?o Isacksson Eduardo Guilherme Gentil de Farias francisco José Dias Roberto Nascimento de Farias Anandra Machado francisco de assis Esteves Mauricio Mussi Molisani Instituto de Biodiversidade e Sustentabilidade (NUPEM/UFRJ) Universidade Federal do Rio de Janeiro Grupo de Gestao Ecologia e Tecnologia Marinha (GTMar)Departamento de Engenharia de Pesca a Ciencias Biologicas (DEPB)Universidade do Estado de Santa Catarina (UESC) Laboratorio de Hidrodinamica Costeira Estuarina e de Aguas Interiores (LHiCEAI)Departamento de Oceanografia e Limnologia (DEOLI)Universidade Federal do Maranhao (UFMA) Programa de Pos-Graduacao em Ciencias Ambientais e Conservacao-PPGCIAC Instituto de Biodiversidade e Sustentabilidade (NUPEM/UFRJ)

Increasing continental suspended sediment influx to coral reefs is an example of land-sea coupling that requires the identification of sources,magnitude of transport,and controlling *** Brazil,a small coastal basin(Macae River) was identified as a source of suspended sediment to a coral reef on the coast of Cape Armacao dos *** suspended sediment loads were measured at the basin as were fluxes within the estuary and towards the coast during eight tidal *** load and yield from this basin were typical of small coastal basins,showing high to moderate slopes and transitional land ***,the magnitude of the river loads was lower than the sediment transport within the estuary,indicating that the estuary amplifies river fluxes and sustains the transference of suspended sediment alongshore to the coral ***,the estuary displays both suspended particle retention and export capacity and,therefore,fluxes to the coast and the coral reef occur as episodic events.

关键词： Suspended matter Watershed Mass-balance budget Campos Basin

Antibacterial enhancement of antibiotic activity by Enterolobium contortisiliquum(Vell.)Morong

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Asian Pacific Journal of Tropical Biomedicine 2017年第10期7卷 945-949页

作者： Zildene de Sousa Silveira Nair Silva Macêdo Thiago Sampaio de Freitas Ana Raquel Pereira da Silva Joycy Francely Sampaio dos santos Maria Flaviana Bezerra Morais-Braga José Galberto Martins da Costa Raimundo Nonato Pereira Teixeira Jean Paul Kamdem Henrique Douglas Melo Coutinho francisco assis Bezerra da Cunha Laboratorio de Bioprospeccao do Semioarido Universidade Regional do CaririCratoBrazil Laboratorio de Microbiologia e Biologia Molecular Universidade Regional do CaririCratoBrazil Laboratorio de Pesquisas de Produtos Naturais Universidade Regional do CaririCratoBrazil departamento de Ciencias biologicas Universidade Regional do Cariri-URCACratoCEP:63105-000Brazil Programa de Pos-graduacao em Bioprospeccao Molecular Universidade Regional do Cariri–URCACratoBrazil

Objective: To identify the main chemical classes of compounds from aqueous extract of Enterolobium contortisiliquum(E. contortisiliquum) seed bark and to evaluate its antibacterial activity, as well as its potential to increase the activity of antibiotics against strains of Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia ***: Different classes of compounds in the aqueous extract of E. contortisiliquum were evaluated based on the visual changes in the coloration and the formation of precipitate after the addition of specific reagents. The antibacterial activity of the extract and its potential to increase of antibiotic activity of antibiotics drugs, gentamicin and norfloxacin was determined by using the microdilution ***: Our results demonstrated that the following secondary metabolites were presented in E. contortisiliquum seed bark: flavones, flavonols, xanthones, flavononols,chalcones, aurones, flavones and catechins. The extract itself had very low antibacterial activity against all bacterial strains tested(MIC ≥ 1024 μg/m L), but there was an increase in the antibiotic activity of gentamicin and norfloxacin when combined in the subinhibitory concentration(i.e., MIC/8).Conclusions: Our data suggests that E. contortisiliquum seed bark may be an alternative source for new drugs with the potential to increase antibiotic activity against different strains of bacteria.

关键词： Antibiotic activity Enhancement Synergism Chemical compounds Bioactivity

Characteristics relating to the interiorization of acquired immunodeficiency syndrome in Brazil: a cross-sectional study

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Infectious Diseases of Poverty 2015年第1期4卷 234-238页

作者： Gabriel de deus Vieira Ana Raquel Paz dos Reis francisco Ormidiel Teles de Alcântara Augusto Karina Reis Martins Paulo Roberto Fernes Kern Thairini Fuza de souza Sérgio de Almeida Basano Luís Marcelo Aranha Camargo Camila Maciel de Sousa departament of Medicine São Lucas CollegePorto VelhoRondôniaBrazil Tropical Medicine Center of Rondônia Porto VelhoRondôniaBrazil Institute of Biomedical Sciences 5 University of São PauloMonte NegroRondôniaBrazil

Background:In recent years there has been changes in the social and geographic profile of acquired immunodeficiency syndrome(AIDS).The aim of this study was to evaluate the internalization of AIDS in the state of Rondônia,***:In Rondônia,1473 AIDS cases were reported,with an average annual incidence of 15.8/100,000 persons(42.7%women).The most common mode of viral transmission was sexual(96.5%),and the majority of the individuals had not completed their primary education(64.8%).There was heterogeneity in relation to case distribution,involving almost all of the municipalities in the *** average annual mortality rate was 2.5/100,000 ***:Rondônia has a higher incidence of AIDS than the national average and the northern *** to provide access to treatment and follow-up of these individuals should be implemented,prioritizing areas where the incidence is higher and decentralizing the treatment of patients with AIDS in the state.

关键词： Epidemiology Aids Residence characteristics Amazo

Climatic Projections of Lightning in Southeastern Brazil Using CMIP5 Models in RCP’s Scenarios 4.5 and 8.5

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American Journal of Climate Change 2017年第3期6卷 539-553页

作者： Ana Paula Paes dos santos Osmar Pinto Júnior Sérgio Rodrigo Quadros dos santos francisco José Lopes de Lima Everaldo Barreiros de souza André Arruda Rodrigues de Morais Eldo E. ávila Analía Pedernera National Institute for Space Research (INPE) S&atilde o José dos Campos Brazil Vale Institute of Technology (ITV) Belém Brazil National University of Cordoba (UNC) Córdoba Argentina

Given the high and increasing lightning incidence over the Southeast of Brazil and the various impacts that this phenomenon generates to society, there is a growing need in predicting its occurrence, in order to minimize its consequences. In this context, this work presents the development of a methodology for the projection of lightning in the State of S?o Paulo (Southeastern Brazil), using the HadGEM2-ES and CSIRO-Mk3.6 models in two IPCC climate change scenarios: RCP4.5 and RCP8.5. Since lightning is not an output variable of climate models, tests were carried out to evaluate the relationship between the observed data of oceanic and atmospheric fields, which are known as outputs of the models, and the lightning from the RINDAT and BrasilDAT detection networks. As result, a correlation of 0.84 was obtained. In the projections, it was verified that, while during a large portion of the current climate we observed events of lightning below the average, the future climate reveals the preponderance of anomalously above average events, both in the scenario of intermediate-low emissions (RCP4.5) and in the scenario of high emissions (RCP8.5), suggesting a change in the pattern of the lightning incidence in the State of S?o Paulo.

关键词： Lightning Climatic Projections Southeastern Brazil

Rainfall variability in the Brazilian northeast biomes and their interactions with meteorological systems and ENSO via CHELSA product

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Big Earth Data 2019年第4期3卷 315-337页

作者： Washington Luiz Félix Correia Filho Joséfrancisco de Oliveira-Júnior Dimas de Barros Santiago Paulo Miguel de Bodas Terassi Paulo Eduardo Teodoro Givanildo de Gois Claudio JoséCavalcante Blanco Pedro Henrique de Almeida souza Micejane da Silva Costa Heliofábio Barros Gomes Paulo Josédos santos Institute of Atmospheric Sciences(ICAT) Federal University of Alagoas(UFAL)MaceióBrazil Postgraduate Program in Biosystems Engineering(PGEB) Federal Fluminense University(UFF)NiteróiBrazil Postgraduate Program in Meteorology Unidade Acadêmica de Ciências Atmosféricas(UACA)Federal Universityof Campina Grande(UFCG)Campina GrandeBrazil Postgraduate Program in Physical Geography University of São Paulo(USP)São PauloBrazil Federal University of Mato Grosso do Sul(UFMS) Chapadão do SulBrazil fSchool of Industrial Metallurgical Engineering of Volta Redonda Technological CenterUniversidade Federal Fluminense(UFF)Volta RedondaBrazil Faculty of Sanitary and Environmental Engineering(FAESA/ITEC) Federal University of Pará(UFPA)BelémBrazil

Brazilian biomes are home to a significant portion of the world’s biodiversity,with a total of 14%of existing species and still concentrate 20%of the world’s water ***,changes in biomes have a direct impact on rainfall patterns and water *** on this,the objective was to evaluate the variability of rainfall in the four existing biomes in the Northeast Brazil(NEB)and their interaction with the ENSO climate variability mode and regional scale meteorological systems via CHELSA *** this,monthly rainfall data were used from 1979 to 2013,with a spatial resolution of 1 km×1 km of the CHELSA product,and seasonal and annual rainfall patterns were extracted via *** was found that the rainy season in the Amazon,Caatinga and Cerrado biomes occurred between January and April,with varying intensities,except for the Atlantic *** seasonality patterns are associated with the NEB meteorological systems,with emphasis on ITCZ(all Biomes),UTCV(Amazon,Caatinga and Cerrado),Frontal Systems(extreme south of Caatinga,Cerrado and Atlantic Forest)and EWD/TWD in the(Atlantic Forest).In the inter-annual scale,the remarkable influence of ENSO was verified,mainly in the years 1983,1985,1989,1993,1998,2009 and *** is noteworthy that 1985 was the wettest year of the period,with a surplus in all biomes,while the driest year differs between the Amazon(1983),Atlantic Forest and Caatinga(1993)and Cerrado(2012)*** study via orbital product in NEB showed that anthropogenic processes and natural variability interfere with the forms of rain interception in the biomes and hence in rainfall patterns and water recycling in NEB.

关键词： Phytophysiognomy rainfall inter-annual variability water recycling climate change land use and occupation

Joannesia princeps： Evaluation of Aqueous Extracts Genotoxicity Utilizing Allium cepa Assay and Micronucleus Test

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Journal of Life Sciences 2013年第12期7卷 1249-1254页

作者： Viviane Moreira de Lima Jessica Tamara dos santos Teixeira Jennifer Vieira Gomes Lelio Kallut Almeida Netto Leonardo Oliveira Bastos Hataanderson Luiz Cabral dos santos Lenicio Gongalves francisco de assis da Silva Bruno Pereira Berto Helcio Resende Borba department of Animal Biology Federal Rural University of Rio de Janeiro Seropddica Rio de Janeiro 23890000 Brazil

Evaluation of the genotoxic and cytotoxic potentials is a part of the analysis process where plant products with therapeutic properties must be submitted for being safely employed, mainly through long time points, doannesia princeps, also known as ＂cotieira＂ has been used on popular medicine as laxative and several diseases treatment. Aiming to analyze genotoxic potential ofd. princeps leaves extract, Allium cepa assay and micronucleus test were employed. No genotoxic activity presented J. princeps leaves extract; however, extracts cytotoxic activity over A. cepa meristematic cells and mice marrow cells was observed. J. princeps leaves extract presented antiproliferative activity at concentrations and systems employed indicating their therapeutic potential for cellular cycle inhibition. Moreover, results showed that in regard to its mutagenic effect, usage of J. princeps leaves tea on popular medicine has been a safe procedure, as well.

关键词： Cytotoxicity genotoxicity Allium cepa assay micronucleus Joannesia princeps.

Identification of Sugar,Amino Acids and Minerals from the Pollen of Jandaíra Stingless Bees(Melipona subnitida)

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Food and Nutrition Sciences 2014年第11期5卷 1015-1021页

作者： Girliane Regina da Silva Tamires Botelho da Natividade Celso Amorim Camara Eva Monica Sarmento da Silva francisco de assis Ribeiro dos santos Tania Maria Sarmento Silva departamento de Ciencias Moleculares Universidade Federal Rural de PernambucoPernambucoBrazil Colegiado de Zootecnia Universidade Federal do Vale de Sao FranciscoPernambucoBrazil departamento de Ciencias Biológicas Universidade Estadual de Feira de SantanaBahiaBrazil

The aim of this investigation was to analyze two samples of pollen from jandaíra stingless bees (Melipona subnitida) in view of their mineral composition, free amino acids and sugars. Palynological analysis showed that the predominant pollen was monofloral from Senna sp. species (94.5%, pollen 2011) and the second pollen sample showed the presence of two primary species, Chamaecrista sp. (39.2% pollen 2009) and Mimosa tenuiflora (43.5%) (pollen 2009). The highest mineral content was potassium. The bee pollen contained 20.8% and 31.0% of mannitol in samples from 2011 and 2009, respectively. Proline and serine are the predominant amino acids. High content of essential amino acids, minerals and the sugar mannitol confirmed high nutritional value of pollen samples from jandaíra bees.

关键词： Jandaíra Bees Mineral Amino Acids Sugar

Characterization of Clinical and Environmental Isolates of <i>Cryptococcus neoformans</i>/<i>Cryptococcus gattii</i>Complex Maintained in Yeast Culture Collection in São Paulo, Brazil

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Open Journal of Epidemiology 2018年第2期8卷 76-92页

作者： Pedro Henrique Magalh&atilde es Cardoso Bosco Christiano Maciel da Silva Jairo Ivo dos santos Rennan Luiz Oliveira dos santos Diniz Pereira Leite Jú nior Marcos Ereno Auler Luciana da Silva Ruiz Eriques Gonç alves da Silva Dé bora Moreira Carina Domaneschi francisco de assis Baroni Marcia de souza Carvalho Melhem Marilena dos Anjos Martins Claudete Rodrigues Paula Faculty of Veterinary Medicine and Zootechny-FMVZ-USP University of S&#227 o Paulo (USP) S&#227 o Paulo Brazil Faculty of Medicine/FMUSP University of S&#227 o Paulo (USP) S&#227 o Paulo Brazil Federal University of Santa Catarina (UFSC) Florian&oacute polis Brazil Faculty of dentistry University of S&#227 o Paulo (FOUSP) S&#227 o Paulo Brazil Faculty of Medicine Federal University of Mato Grosso (UFMT) Cuiab&aacute Brazil Faculty of Pharmacy University of the Western Center of Paran&aacute (UNICENTRO) Guarapuava Brazil Institute Adolfo Lutz (IAL) Bauru Brazil Federal Rural University of Rio de Janeiro (UFRRJ) Serop&eacute dica Brazil Institute Adolfo Lutz (IAL) S&#227 o Paulo Brazil

Objective: As isolates of Cryptococcus are frequently kept collection stocks in institutions, sometimes without proper characterization, we sought to determine the genotype profiles, protease and phospholipase activities “in vitro” and the susceptibility testing for azoles and amphotericin B. Methodology: 84 isolates from several regions of Brazil (40 samples from clinical origin and 44 isolates from environmental origin) were maintained at the microorganism’s bank of the Biomedical Science Institute (ICB-USP) of the São Paulo University, in São Paulo, Brazil. This isolates was submitted fungal strains determination, DNA extraction and purification, determination of genotype by URA5 gene RFLP of CGB-positive isolates, protease and phospholipase activity and susceptibility to antifungals. Results: Of six CGB positive isolates tested by RFLP-PCR, only four presented a genomic profile consistent C. gattii species (VGII), while two other were C. neoformans (VNI and VNIII), indicating the existence of canavanine-resistante C. neoformans isolates in the culture collections. The clinical isolates secreted higher levels of phospholipase and environmental isolates but no differences were observed for the protease levels. Almost all isolates were sensible to azoles and amphotericin B. Conclusion: We point out in this research the existence of C. neoformans strains resistant to canavanine and intrinsic characteristic of C. gatti. These results demonstrate the importance to perform a detailed characterization of isolates kept in culture collections.

关键词： Cryptococcus Complex Genotyping Protease Phospholipase Etest Culture Collection