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Concentration and Preservation of Very Low Abundance Biomarkers in Urine, such as Human Growth Hormone (hGH), by Cibacron Blue F3G-A Loaded Hydrogel Particles

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Nano Research 2008年第6期1卷 502-518页

作者： Claudia Fredolini francesco meani K.Alex Reeder Sally Rucker Alexis Patanarut Palma J.Botterell Barney Bishop Caterina Longo Virginia Espina Emanuel F.Petricoin III Lance A.Liotta Alessandra Luchini Department of Urology S.Giovanni Bosco HospitalTorino 10154Italy Department of Medicine and Experimental Oncology University of TurinTurin 10126Italy Gynecology and Obstetrics Department University of BresciaBrescia 25100Italy Department of Dermatology and Venereology University of Modena and Reggio EmiliaModena 41100Italy Center for the Applied Proteomics and Molecular Medicine George Mason UniversityManassasVA 20110USA Chemistry and Biochemistry Department George Mason UniversityManassasVA 20110USA.

Urine is a potential source of diagnostic biomarkers for detection of diseases,and is a very attractive means of non-invasive biospecimen ***,proteomic measurement in urine is very challenging because diagnostic biomarkers exist in very low concentration(usually below the sensitivity of common immunoassays)and may be subject to rapid *** nanoparticles functionalized with Cibacron Blue F3G-A(CB)have been applied to address these challenges for urine biomarker *** chose one of the most difficult low abundance,but medically relevant,hormones in the urine:human growth hormone(hGH).The normal range of hGH in serum is 1 to 10 ng/mL but the urine concentration is suspected to be a thousand times less,well below the detection limit(50 pg/mL)of sensitive clinical hGH *** demonstrate that CB particles can capture,preserve and concentrate hGH in urine at physiological salt and urea concentrations,so that hGH can be measured in the linear range of a clinical immunometric *** and cadaveric hGH were captured from synthetic and human urine,concentrated and measured with an Immulite chemiluminescent *** of hGH less than 0.05 ng/mL(the Immulite detection limit)were concentrated to 2 ng/mL,with a urine volume of 1 *** response studies using 10 mL of urine demonstrated that the concentration of hGH in the particle eluate was linearly dependent on the concentration of hGH in the starting solution,and that all hGH was removed from *** if the starting urine volume is 100 mL,the detection limit will be 0.1 pg/*** from a healthy donor whose serum hGH concentration was 1.34 ng/mL was studied in order detect endogenous *** from a volume of 33 mL,the particle eluate had an hGH concentration of 58 pg/mL,giving an estimated initial concentration of hGH in urine of 0.175 pg/*** nanotechnology described here appears to have the desired precision,accuracy and sensitivity to suppor

关键词： Urine proteomics Cibacron Blue F3G-A human growth hormone hydrogel nanoparticles N-isopropylacrylamide

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