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Increased expression of regulatory T cell-associated markers in recent-onset diabetic children

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Open Journal of Immunology 2011年第3期1卷 57-64页

作者： Mikael Pihl Mikael Chéramy Jenny Mjosberg Johnny Ludvigsson Rosaura casas Division of Pediatrics and Diabetes Research Center Department of Clinical and Experimental MedicineLinkoping UniversityLinkopingSweden Division of Clinical Immunology Department of Clinical and Experimental MedicineLinkoping UniversityLinkopingSweden

CD4+CD25hi T cells are thought to be crucial for the maintenance of immunological tolerance to self antigens. In this study, we investigated the frequencies of these cells in the early stage of type 1 diabetes, as well as in a setting of possible pre-diabetic autoimmunity. Hence, the expression of FOXP3, CTLA-4, and CD27 in CD4+ CD25hi T cells was analyzed using flow cytometry in 14 patients with recent onset type 1 diabetes, in 9 at-risk individuals, and 9 healthy individuals with no known risk for type 1 diabetes. Our results show there were no differences in the frequency of CD4+CD25hi cells between groups. However, compared to controls, recent-onset type 1 diabetic patients had higher expression of FOXP3, CTLA-4, and CD27 in CD4+ CD25hi cells from peripheral blood. The median fluorescence intensity of FOXP3 was significantly higher in CD4+CD25hi cells from patients with type 1 diabetes than from controls. Furthermore, a positive correlation between the frequency of FOXP3+ cells and the median fluorescence intensity of FOXP3 was observed among patients with type 1 diabetes. These data suggest that the frequency of CD4+CD25hi FOXP3+ T cells in the periphery is not decreased but rather increased at onset of type 1 diabetes. Thus, functional deficiencies rather than reduced numbers of CD4+CD25hi cells could contribute to the development of type 1 diabetes.

关键词： Regulatory T cells Type 1 Diabetes Autoantibodies

Adapting the Servqual Scale to a Private Hospital Emergency Services： An Empirical Investigation

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Chinese Business Review 2014年第5期13卷 273-289页

作者： Luis E. Ibarra Vanessa casas M Ana L. Partida Universidad Estatal de Sonora Hermosillo Mexico

Most of the time, clients only have one opportunity to evaluate a service received in terms of quality and satisfaction; under this premise organizations have been concerned with developing, in a permanent manner, a quality evaluation culture in the services offered to their clients. To do this, procedures and methods that let them achieve it, have been implemented. That is the case of the model that concedes to measure and evaluate service quality, which is redefined and named Servqual, considering a multidimensional theoretical construct that explains the client＇s perception of quality service considering the differences between what is expected and what is received. Therefore, the goal of this study is to identify the factors that define the quality of emergency services provided by a private hospital in Hermosillo, Sonora, Mtxico, a sample of 384 patients, with a confidence level of 95% and a margin of permissible error of 5%. A questionnaire with 22 items was applied to measure the perceptions and expectations of users in terms of the quality of the service, which was subjected to an extensive evaluation of reliability and construct validity, with an estimate Cronbach＇s alpha of 95.6% and 97.9% respectively. The results showed that Servqual is a valid, reliable, and dependable instrument to monitor and measure the quality of the services offered in private hospitals in Hermosillo, and permit hospital administrators to identify opportunities or improvement areas, from the patients＇ perspective.

关键词： methodology Servqual quality of service level of satisfaction perceptions and expectations of the users

Naringenin prevents experimental liver fibrosis by blocking TGFβ-Smad3 and JNK-Smad3 pathways

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World Journal of Gastroenterology 2017年第24期23卷 4354-4368页

作者： Erika Hernández-Aquino Natanael Zarco Sael casas-Grajales Erika Ramos-Tovar Rosa E Flores-Beltrán Jonathan Arauz Mineko Shibayama Liliana Favari Víctor Tsutsumi José Segovia Pablo Muriel Laboratory of Experimental Hepatology Department of PharmacologyCinvestav-IPNApartado Postal 14-740Mexico City Department of Physiology Biophysics and NeurosciencesCinvestav-IPNApartado Postal 14-740Mexico City Department of Pharmacology School of MedicineAutonomous University of Baja CaliforniaMexicaliApartado Postal 21100 Department of Infectomics and Molecular Pathogenesis Cinvestav-IPNApartado Postal 14-740Mexico City

To study the molecular mechanisms involved in the hepatoprotective effects of naringenin (NAR) on carbon tetrachloride (CCl4)-induced liver fibrosis. METHODSThirty-two male Wistar rats (120-150 g) were randomly divided into four groups: (1) a control group (n = 8) that received 0.7% carboxy methyl-cellulose (NAR vehicle) 1 mL/daily p.o.; (2) a CCl4 group (n = 8) that received 400 mg of CCl4/kg body weight i.p. 3 times a week for 8 wk; (3) a CCl4 + NAR (n = 8) group that received 400 mg of CCl4/kg body weight i.p. 3 times a week for 8 wk and 100 mg of NAR/kg body weight daily for 8 wk p.o.; and (4) an NAR group (n = 8) that received 100 mg of NAR/kg body weight daily for 8 wk p.o. After the experimental period, animals were sacrificed under ketamine and xylazine anesthesia. Liver damage markers such as alanine aminotransferase (ALT), alkaline phosphatase (AP), γ-glutamyl transpeptidase (γ-GTP), reduced glutathione (GSH), glycogen content, lipid peroxidation (LPO) and collagen content were measured. The enzymatic activity of glutathione peroxidase (GPx) was assessed. Liver histopathology was performed utilizing Masson’s trichrome and hematoxylin-eosin stains. Zymography assays for MMP-9 and MMP-2 were carried out. Hepatic TGF-β, α-SMA, CTGF, Col-I, MMP-13, NF-κB, IL-1, IL-10, Smad7, Smad3, pSmad3 and pJNK proteins were detected via western blot. RESULTSNAR administration prevented increases in ALT, AP, γ-GTP, and GPx enzymatic activity; depletion of GSH and glycogen; and increases in LPO and collagen produced by chronic CCl4 intoxication (P 4. Although zymography assays showed that CCl4 produced an increase in MMP-9 and MMP-2 gelatinase activity; interestingly, NAR administration was associated with normal MMP-9 and MMP-2 activity (P < 0.05). The anti-inflammatory, antine

关键词： Fibrosis Transforming growth factor-β Naringenin pSmad3 Smad3 JNK Nuclear factor kappa Carbon tetrachloride

Childhood trauma and factors associated with depression among inpatients with cardiovascular disease

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World Journal of Psychiatry 2017年第2期7卷 106-113页

作者： Felipe José Nascimento Barreto Frederico Duarte Garcia Paulo Henrique Teixeira Prado Paulo Marcos Brasil Rocha Nádia Souza Las casas Felipe Barbosa Vallt Humberto Correa Maila Castro Lourenco Neves Department of Mental Health Federal University of Minas Gerais Postgraduation Program in Molecular Medicine School of Medicine Federal University of Minas Gerais Molecular Medicine National Institute of Science and Technology Avenida Alfredo Balena Federal University of Minas Gerais Hospital das Clínicas Federal University of Minas Gerais

AIM To identify factors associated with depressive symptoms among inpatients with cardiovascular disease(CVD). METHODS This is a cross-sectional study performed in a subsample of a large cross-sectional research that investigated affective disorders and suicide behaviour among inpatients hospitalized in non-surgical wards of the University Hospital of the Federal University of Minas Gerais from November 2013 to October 2015. Sociodemographic and clinical data were obtained through a structured interview and medical record review. Depression was assessed by the depression subscale of the Hospital Anxiety and Depression Scale, with scores ≥ 8 considered as positive screening for depression. We used the Fagestr?m Test for Nicotine Dependence to characterize nicotine dependence. For assessing resilience and early-life trauma, we used the raw scores of the Wagnild and Young Resilience Scale and Childhood Trauma Questionnaire, *** At endpoint, we included 137 subjects. Thirty-eight(27.7%) subjects presented depressive symptoms and nine(23.7%) of those were receiving antidepressant treatment during hospitalization. The female sex; a lower mean educational level; a greater prevalence of previous suicide attempts; a higher level of pain; a higher prevalence of family antecedents of mental disorders; a lower resilience score; and higher childhood trauma score were the factors significantly associated with screening positive for major depression(P < 0.05). Multivariate analysis demonstrated that the factors independently associated with the depressive symptoms were a higher childhood trauma severity(OR = 1.06; P = 0.004); moderate to severe nicotine dependence(OR = 8.58; P = 0.008); and the number of previous hospital admissions(OR = 1.11; P = 0.034). The obtained logistic model was considered valid, indicating that the three factors together distinguished between having or not depressive symptoms, and correctly classified 74.6% of individuals in the ***

关键词： Inpatients Depression Cardiovascular disease Depressive symptoms General hospital

Candidate genes underlying QTL for flowering time and their interactions in a wide spring barley(Hordeum vulgare L.) cross

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The Crop Journal 2021年第4期9卷 862-872页

作者： Ana M.casas Carlota R.Gazulla Arantxa Monteagudo Carlos P.Cantalapiedra Marian Moralejo MPilar Gracia Francisco J.Ciudad William T.B.Thomas Jose L.Molina-Cano Scott Boden Bruno Contreras-Moreira Ernesto Igartua Department of Genetics and Plant Production Aula Dei Experimental Station(EEAD-CSIC)Avda Montanana 1005E-50059 ZaragozaSpain University of Lleida-AGROTECNIO Center Avda.Rovira Roure 19125198 LleidaSpain Instituto de Tecnologia Agraria Junta de Castilla y LeonITACyLP.O.Box 17247071 ValladolidSpain The James Hutton Institute InvergowrieDundee DD25DAUK Institut de Recerca i Tecnologia Agroalimentaries(UdL-IRTA) Avda Rovira Roure 19125198 LleidaSpain John Innes Centre Norwich Research ParkNorwich NR47UHUK Fundacion ARAID calle Maria de Luna 1150018 ZaragozaSpain Present address:Centro de Biotecnologia y Genomica de Plantas Universidad Politecnica de Madrid(UPM)and Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria(INIA)Madrid 28223Spain Present address:School of Agriculture Food and WineWaite Research InstituteUniversity of AdelaideGlen OsmondSA 5064Australia Present address:The European Bioinformatics Institute(EMBL-EBI) Wellcome Trust Genome CampusHinxtonUK

Response to vernalization and photoperiod are the main determinants controlling the time to flowering in temperate cereals. While the individual genes that determine a plant's response to these environmental signals are well characterized, the combinatorial effect on flowering time of allelic variants for multiple genes remains unresolved. This study investigated the genetic control of flowering-time in a biparental population of spring barley, derived from a wide cross between a late-flowering European and an early-flowering North-American cultivar. While the major flowering time genes are not segregating in the Beka ×Logan cross, large variation in flowering was observed. We identified five QTL, with both parents found to contribute early alleles. The catalog of QTL discovered aligns with several candidate genes affecting flowering time in barley. The combination of particular alleles at HvCEN, HvELF3 and HvFT1 in Logan are responsible for the earliness of this cultivar. Interestingly, earliness for flowering could be further enhanced, with Beka found to contribute three early alleles, including a QTL colocating with a HvFD-like gene, suggesting that there are diverse aspects of the flowering-time pathway that have been manipulated in these two cultivars. Epistatic interactions between flowering-time QTL or candidate genes were observed in field data and confirmed under controlled conditions. The results of this study link photoperiod-dependent flowering-time genes with earliness per se genes into a single model, thus providing a unique framework that can be used by geneticists and breeders to optimize flowering time in barley.

关键词： Barley Flowering HvELF3 HvCEN HvFT1

盐盆石盐的成岩作用:现代石盐、第四纪石盐和二叠纪石盐岩相特征的比较

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地质地球化学 1993年第4期21卷 35-38,8页

作者： E.casas T.K.Lowenstein 袁复怀许志强中科院盐湖研究所

现代盐盆石盐(加州萨林河谷、墨西哥南加利福尼亚州萨林纳奥莫特皮克)和第四纪浅埋藏(0～200m)石盐(加州萨林河谷和布里斯托尔干湖、中国柴达木盆地察尔汗湖、玻利维亚乌尤尼湖)的岩相研究表明,石盐的成岩改造与沉积作用是同时开始的,... 详细信息

现代盐盆石盐(加州萨林河谷、墨西哥南加利福尼亚州萨林纳奥莫特皮克)和第四纪浅埋藏(0～200m)石盐(加州萨林河谷和布里斯托尔干湖、中国柴达木盆地察尔汗湖、玻利维亚乌尤尼湖)的岩相研究表明,石盐的成岩改造与沉积作用是同时开始的,在埋藏的上部几米内这种成岩改造很强烈,并在埋藏的最初45m内基本上是完全的。经历过反复洪水期、蒸发浓缩和干化的现代盐盆的石盐壳,具有丰富的同沉积成岩特征。这些“成熟的”现代石盐的组构都是以(洪水期形成的)结构和组构以及(干化期形成的)胶结结构为主。泥质夹层含有数量不等的置换石盐晶体。在浅埋藏处,石盐保留着“成熟的”现代盐盆石盐的许多结构特征。位于最上面几米之下的石盐都已不容易被洪水所溶解,而继续被纯洁石盐胶结着。在埋藏的最初10m内,胶结作用使石盐盐壳的孔隙度减小到不足10％。剩下的空隙都由于埋藏深度达到45m后被全部填满。在埋深浅的情况下,置换石盐继续在泥中生长,但可能只局限于最初的几十米埋藏深度内。胶结作用和浅埋藏的置换石盐生长的机制可能包括:(1)地下卤水的蒸发浓缩;(2)当卤水下渗到沉积物表面之下时地表卤水的冷却。新墨西哥州二叠纪萨拉多和鲁斯特物两建造的未变形石盐具有的精细同沉积结构和丰富的纯洁石盐胶结物,都可与现代?

关键词：石盐成岩作用岩相第四纪二叠纪

Current trends in access to treatment for hepatitis B in immigrants vs non-immigrants

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Gastroenterology Report 2020年第5期8卷 362-366,I0002页

作者： Mireia Miquel Albert Pardo Montse Forné Gemma Martínez-Alpin AdriàRodríguez-Castellano Meritxell casas MercèRosinach MercèRoget Blai Dalmau Rocío Temiño Joan Carlos Quer Jordi Sanchez-Delgado Jordi Ortiz Mercedes Vergara Liver Unit Gastroenterology DepartmentParc TaulíUniversity HospitalInstitut d’Investigacio i InnovacióParc TaulíI3PTAutonomous University of BarcelonaSabadellSpain Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas(CIBERehd) Instituto de Salud Carlos IIIMadridSpain Gastroenterology Department University Hospital Joan XXIIITarragonaSpain Liver Unit Gastroenterology DepartmentUniversity Hospital Mútua TerrassaBarcelonaSpain Liver Unit Gastroenterology DepartmentConsorci Sanitari de TerrassaTerrassaSpainó

Background:Universal vaccination for hepatitis B virus(HBV)and migratory movements have changed the demographic characteristics of this disease in Spain and in ***,we evaluated the characteristics of the disease and the possible differences according to origin(immigrants vs non-immigrants)and access to ***:This is a multicenter cross-sectional study(June 2014 to May 2015)in which outpatients with a positive HBsAg were seen and followed in four Hepatology *** and clinical data and indication and access to treatment were collected in two different regions of Catalonia(Spain)where there are no barriers to treatment due to a comprehensive coverage under the National Health ***:A total of 951 patients were evaluated(48.1%men).Of these,46.6%were immigrants(58.7%of them were born in Africa)and were significantly younger compared to *** proportions of patients with alcohol consumption,being overweight,and other indicators of metabolic co-morbidities were significantly higher in *** the 937 patients receiving HBeAg examination,91.7%were *** HBeAg-positive infection was significantly higher in immigrants(3.9%vs 0.6%,P=0.001)and chronic HBeAg-negative hepatitis was higher non-immigrants(31.7%vs 21.4%,P<0.001).Not only was the proportion of patients who met treatment criteria significantly higher among nonimmigrants(38.4%vs 29.2%,P=0.003),but also the proportion of those with indication of effectively receiving therapy at the time of data collection(83.2%vs 57.8%,P<0.001).Conclusions:The immigrant population with HBV is younger and has a lower prevalence of metabolic co-morbidities and a higher frequency of chronic HBeAg *** having access to care and an indication for treatment,some do not get adequately treated due to several factors including local adaptation that precludes access to treatment.

关键词： hepatitis B prevalence immigration access to treatment

Two novel mutations in theGAN gene causing giant axonal neuropathy

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World Journal of Pediatrics 2018年第3期14卷 298-304页

作者： Monica Irad Normendez-Martínez Lucero Monterde-Cruz Roberto Martínez Magdalena Marquez-Harper Nayelli Esquitin-Garduno Margarita Valdes-Flores Leonora casas-Avila Valeria Ponce de Leon-Suarez Viktor Javier Romero-Díaz Alberto Hidalgo-Bravo Medical Specialties Hospital Boulevard Milenio 130San Carlos la Roncha37660 LeonGuanajuatoMexico Department of Gene tics National Institute of R ehabilitationAvenue Mexico-Xochimilco 28914389 Me xico CityMexico Department ofHis tology School ofMedicineAutonomous University oft heS tate ofN uevo LeonMonterreyMexico

Background Giant axonal neuropathy (GAN) is a rare neurodegenerative disease transmitted in an autosomal recessive mode. This disorder presents motor and sensitive symptoms with an onset in early childhood. Progressive neurodegeneration makes the patients wheelchair dependent by the end of the second decade of life. Affected individuals do not survive beyond the third decade of life. Molecular analysis has identified mutations in the gene GAN in patients with this disorder. This gene produces a protein called gigaxonin which is presumably involved in protein degradation via the ubiquitin-proteasome system. However, the underlying molecular mechanism is not clearly understood yet. Methods Here we present the fi rst patient from Mexico with clinical data suggesting GAN. Sequencing of the GAN gene was carried out. Changes in the nucleotide sequence were investigated for their possible impact on protein function and structure using the publicly available prediction tools PolyPhen-2 and PANTHER. Results The patient is a compound heterozygous carrying two novel mutations in the GAN gene. The sequence analy-sis revealed two missense mutations in the Kelch repeats domain. In one allele, a C>T transition was found in exon 9 at the nucleotide position 55393 (g.55393C>T). In the other allele, a transversion G>T in exon 11 at the nucleotide position 67471 (g.67471G>T) was observed. Both of the bioinformatic tools predicted that these amino acid substitutions would have a negative impact on gigaxonin's function. Conclusion This work provides useful information for health professionals and expands the spectrum of disease-causing mutations in the GAN gene and it is the first documented case in Mexican population.

关键词： GAN Genetics Giant axonal neuropathy Incapacity Neurodegenerative

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喧嚣之外的宁静圣保罗BD住宅

室内设计与装修 2022年第10期 52-57页

作者： [巴西]studio arthur casas 悠扬(编译) studio arthur casas

本案是由巴西设计公司Arthur casas设计完成的一栋私人住宅,主人是一对年轻夫妇和他们的两个小孩。为了在有限的地块上建造更多的空间,设计师决定沿垂直方向进行布局,并通过电梯与楼梯连接每个楼层。建筑的外墙采用现浇混凝土。其色彩... 详细信息

本案是由巴西设计公司Arthur casas设计完成的一栋私人住宅,主人是一对年轻夫妇和他们的两个小孩。为了在有限的地块上建造更多的空间,设计师决定沿垂直方向进行布局,并通过电梯与楼梯连接每个楼层。建筑的外墙采用现浇混凝土。其色彩经过多次测试和验证,最终确定为赭石,它不仅使建筑更好地融入环境,而且也与建筑的整体风格完美匹配。

关键词：私人住宅现浇混凝土电梯融入环境整体风格楼梯完美匹配外墙