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国外医学（医学地理分册） 1991年第4期12卷 162-163页

作者： Solo.,nw 孙晓红

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新的API泵挂深度推荐算法简述

油气田开发工程译丛 1993年第7期 37-40页

作者： H.,nw,Jr 雷小强

Biocompatibility evaluation in vitro. Part I: Morphology expression and proliferation of human and rat osteoblasts on the biomaterials

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Journal of Central South University of Technology 2001年第1期8卷 1-8页

作者： RUAN Jian ming 1,GRANT M. Helen 2 (1 Powder Metallurgy State Key Laboratory, Central South University, Changsha 410083, China 2 Bioengineering Unit, Strathclyde University, Glasgow, G4 0nw, UK) Powder Metallurgy State Key Laboratory Central South University Changsha China Bioengineering Unit Strathclyde University Glasgow UK

The biocompatibility evaluation of calcium phosphate based biomaterials is performed by tissue culture in vitro model. Three kinds of bioceramic materials which are potential to deal with bone trauma and/or conduct tissue growth are recommodated. The biological research results show that human and animal osteoblast cells anchor the materials surface in two hours in culture. Confocal laser scanning microscopy (CLSM) demonstrated the normal cell distribution and proliferation on both of dense and porous biomaterials. Hydroxyapatite and tricalcium phosphate stimulate cell proliferation. However, DNA and protein synthesis were considerably limited and the apoptosis phenomenon would be present on the hydroxyapatite (HA) materials by adding Al, Mg elements. Several important methods of biocompatibility evaluation of implant materials are described and the related biological molecular techniques such as tissue culture, cell transfection, cellular DNA stain, and Lowry assay are involved in the present research.

关键词： biomaterials tissue culture cell morphology proliferation

Biocompatibility evaluation in vitro. Part III: Cytotoxicity expression of human and animal osteoblasts on the biomaterials

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Journal of Central South University 2001年第3期13卷 147-155页

作者： RUAN Jian-ming 1,M.H. Grant 2 (1. State Key Laboratory for Powder Metallurgy, Central South University, Changs ha 410083, China 2. Bioengineering Unit, Strathclyde University, Glasgow, G4 0nw, UK) State Key Laboratory for Powder Metallurgy Central South University Changsha China Bioengineering Unit Strathclyde University Glasgow UK

Exclusion and inflammation in the clinic are observe d for various reas ons including material che- mical composition, physical properties as well a s macr o- and micro-structure of the implants, surface condition of the implants, and also patient dependent factors. Cytotoxicity expression of cells is a central issue i n current biocompatibility to screen the potential implant materials and drugs. This study was aimed at investigation reaction between the potential implant mat erials and surround tissue. Cytotoxicity of human and rat osteoblast in the mate rial extracts was determinated by testing standards such as GHS assay, MTT assay , alkaline phosphatase activity assay, LDH assay, and Lowry assay. Research resu lts demonstrated that compared with the control condition polystyrene culture pl a te both human and rat osteoblast cells have normal phenotypic expression in hydr oxyapatite extract, and this expression was statistically restricted in hydroxya patite-spinel extract. How- ever, this restrict, e.g. cytotoxicity could be partially eliminated by immersion treatment of the materials in culture medium.

关键词： biomaterial cytotoxicity biological molecular technique

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对付业务中的欺骗行为

当代保险 1991年第6期 36-38页

作者：金，nw

Biocompatibility evaluation in vitro. Part II: Functional expression of human and animal osteoblasts on the biomaterials

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Journal of Central South University of Technology 2001年第2期8卷 75-82页

作者： RUAN Jian ming 1,GRANT M. Helen 2 (1.State Key Laboratory for Powder Metallurgy, Central South University, Changsha 410083, China 2.Bioengineering Unit, Strathclyde University, Glasgow, G4 0nw, UK) State Key Laboratory for Powder Metallurgy Central South University Changsha China Bioengineering Unit Strathclyde University Glasgow UK

DNA synthesis and collagen formations on the implant material by cell culture in vitro are the most important phenotypical expression to estimate the biocompatibility. In this part, DNA synthesis and collagen formation on implant materials were quantitatively and qualitatively estimated by radioactive isotope H + thymidine to incorporate into DNA chains, H + proline to incorporate into type I collagen proteins followed by scin tillation counting and antibody antigen immunocytochemistry staining, respectively. Research results demonstrate that hydroxyapatite (HA) stimulates DNA synthesis and collagen formation on the material whereas this stimulation is restricted by adding spinel to the materials. There are statistical differences between the influences of material components on both DNA synthesis and collagen formation. It is supposed that porous materials can supply more platforms for cell anchoring, and more DNA and collagen are synthesised on the porous materials. Immersion in culture medium results in new HA crystal formation on the porous HA materials.

关键词： biocompatibility DNA synthesis collagen protein incorporation

Improving reservoir volumetric estimations in petroleum resource assessment using discovery process models

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Petroleum Science 2009年第2期6卷 105-118页

作者： Osadetz Kirk G. Geological Survey of Canada

The reservoir volumetric approach represents a widely accepted, but flawed method of petroleum play resource calculation. In this paper, we propose a combination of techniques that can improve the applicability and quality of the resource estimation. These techniques include： 1） the use of the Multivariate Discovery Process model （MDP） to derive unbiased distribution parameters of reservoir volumetric variables and to reveal correlations among the variables; 2） the use of the Geo-anchored method to estimate simultaneously the number of oil and gas pools in the same play; and 3） the crossvalidation of assessment results from different methods. These techniques are illustrated by using an example of crude oil and natural gas resource assessment of the Sverdrup Basin, Canadian Archipelago. The example shows that when direct volumetric measurements of the untested prospects are not available, the MDP model can help derive unbiased estimates of the distribution parameters by using information from the discovered oil and gas accumulations. It also shows that an estimation of the number of oil and gas accumulations and associated size ranges from a discovery process model can provide an alternative and efficient approach when inadequate geological data hinder the estimation. Cross-examination of assessment results derived using different methods allows one to focus on and analyze the causes for the major differences, thus providing a more reliable assessment outcome.

关键词： Multivariate Discovery Process model sampling bias correction cross-validation Geoanchored method