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Arabidopsis Protein Phosphatase 2C ABI1 Interacts with Type I ACC Synthases and Is Involved in the Regulation of Ozone-Induced Ethylene Biosynthesis

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Molecular Plant 2014年第6期7卷 960-976页

作者： Agnieszka Ludwikow Agata Ciesla Anna Kasprowicz-Malusk filip mitula Malgorzata Tajdel lukasz Gaigarnski Piotr A. Ziolkowski Piotr Kubiak Arleta Malecka Aneta Piechalak Marta Szabat Alicja Gorska Maciej Dabrowskia Izabela Ibragimow Jan Sadowski Department of Biotechnology Institute of Molecular Biology and Biotechnology Faculty of Biology Adam Mickiewicz University inPoznan Umultowska 89 61-614 Poznan Poland Institute of Plant Genetics Polish Academy of Science Strzeszynska 34 60-479 Poznan Poland Department of Molecular and Cellular Biology Institute of Molecular Biology and Biotechnology Faculty of Biology Adam MickiewiczUniversity in Poznan Umultowska 89 61-614 Poznan Poland Department of Biotechnology and Food Microbiology University of Life Sciences Wojska Polskiego 48 60-627 Poznan Poland Department of Biochemistry Institute of Molecular Bioloqy and Biotechnology Faculty of Bioloqy Adam Mickiewicz University inPoznan Umultowska 89 61-614 Poznan Poland

Ethylene plays a crucial role in various biological processes and therefore its biosynthesis is strictly regu- lated by multiple mechanisms. Posttranslational regulation, which is pivotal in controlling ethylene biosynthesis, impacts 1-aminocyclopropane 1-carboxylate synthase （ACS） protein stability via the complex interplay of specific factors. Here, we show that the Arabidopsis thaliana protein phosphatase type 2C, ABI1, a negative regulator of abscisic acid signaling, is involved in the regulation of ethylene biosynthesis under oxidative stress conditions. We found that ABI1 interacts with ACS6 and dephosphorylates its C-terminal fragment, a target of the stress-responsive mitogen-activated protein kinase, MPK6. In addition, ABI1 controls MPK6 activity directly and by this means also affects the ACS6 phosphorylation level. Consistently with this, ozone-induced ethylene production was significantly higher in an ABI1 knockout strain （abiltd） than in wild-type plants. Importantly, an increase in stress-induced ethylene production in the abiltd mutant was compen- sated by a higher ascorbate redox state and elevated antioxidant activities. Overall, the results of this study provide evi- dence that ABI1 restricts ethylene synthesis by affecting the activity of ACS6. The ABI1 contribution to stress phenotype underpins its role in the interplay between the abscisic acid （ABA） and ethylene signaling pathways.

关键词： hormonal regulation protein phosphorylation/dephosphorylation signal transduction Arabidopsis.

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Phenotyping floral attractiveness to pollinators using volatilomics,3D imaging,and insect monitoring

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Molecular Plant 2024年第8期17卷 1164-1166页

作者： filip Slavkovic Adnane Boualem Catherine Dogimont Abdelhafid Bendahmane institute of Plant Sciences Paris-Saclay(IPS2) INRAECNRSUniversity of Paris-SaclayUniversity of EvryUniversity of Paris CiteBatiment 63091192 GifsurYvetteFrance INRAE UR 1052Unite de Génetique et d'Amelioration des Fruits et LegumesBP94.84143Montfavet.France

PLANTS,POLLINATORS,AND BIODIVERSITY:THESTATUSQUO Plant-pollinator coevolution has played a crucial role in shaping the biodiversity of ecosystems that we know ***,plants and pollinators are key to agriculture,contributing to the production of most fruits and vegetables necessary for healthy human ***,over the last decades,there is mounting evidence of pollinator decline all over the world,which constitutes a major threat to food security(European Commission,2020).To pollinators,nectar and pollen are the main rewards:pollen is essentially their only source of proteins,lipids,and vitamins,while nectar is a carbohydrate-rich solution that they use to fuel somatic functions(Ollerton,2021).However,nectar is much more than just a sweet solution:it comprises a plethora of secondary metabolites and volatiles of major importance for plant-pollinator ***,by neglecting these traits and reducing genetic diversity,plant breeding has potentially increased the risk of losing the traits beneficial to pollinators.

关键词： breeding mount vitamin

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Birds in rocky habitats of the Tatra Mountains (Carpathians)： species diversity and multiple ecological relationships

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Journal of Mountain Science 2016年第6期13卷 1078-1084页

作者： Michal CIACH filip KOWALSKI Department of Forest Biodiversity Institute of Forest Ecology and SilvicultureFaculty of ForestryUniversity of Agricultureal.29 Listopada 4531-425 KrakowPoland

Rocky habitats are regarded as biodiversity hot-spots. Along with high species diversity, diverse ecological relationships can be observed in these habitats. Large groups of bird species use rocks in various ways： as perching/roosting sites, breeding or foraging habitats, information exchange sites, display arenas or as sources of minerals and water. Because of the inaccessibility of these environments, their role and importance to animals has been underestimated. We evaluated the use of rocky habitats by birds in the Tatra Mountains （49°13′N; 19°57′E, Carpathians, central Europe）. Rocky habitats were used by 29 bird species, eight of which used cliffs directly （i.e. for nesting, foraging or resting）. The number of species recorded as using cliffs was correlated with the surface area of the cliff face. A total of 2o forms of rocky habitat use were recorded, in five behavioural categories： vocalization, foraging, perching, flight and nesting. Prevailing behaviours were flying by a rock face, circling above the face, and vocalization on a tree/shrub growing next to a rock. Rocks provide a nesting habitat for specialized petrophilic species and permit the existence of numerous ecological relations between species and habitats. The results of this study show that rocky habitats support the diversity of ecological relationships.

关键词： Bird community Rock Cliff Mountains Alpine species Ecological relationships

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Adaptive slices for acquisition of anisotropic BRDF

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Computational Visual Media 2018年第1期4卷 55-69页

作者： Radomfr Vavra Jiri filip Institute of Information Theory and Automation of the CAS Pod Vodarenskou vezi4185 08Praha 8Czech Republic

BRDF continues to be used as a fundamental tool for representing material appearance in computer graphics. In this paper we present a practical adaptive method for acquisition of anisotropic BRDF, based on sparse adaptive measurement of the complete four-dimensional BRDF space by means of one-dimensional slices, which form a sparse fourdimensional structure in the BRDF space, and can be measured by continuous movements of a light source and sensor. Such a sampling approach is advantageous especially for gonioreflectometer-based measurement devices where the mechanical travel of a light source and a sensor imposes a significant time constraint. In order to evaluate our method, we have performed adaptive measurements of three materials and we simulated adaptive measurements of thirteen others. This method has one quarter the reconstruction error of that resulting from regular non-adaptive BRDF measurements using the same number of measured samples. Our method is almost twice as good as a previous adaptive method, and it requires from two to five times fewer samples to achieve the same results as alternative approaches.

关键词： anisotropic BRDF slice sampling

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Structural and functional aspects of the liver and liver sinusoidal cells in relation to colon carcinoma metastasis

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World Journal of Gastroenterology 2005年第33期11卷 5095-5102页

作者： Katrien Vekemans filip Braet Centre of Experimental Surgery and Anaesthesia Abdominal Transplant Surgery Department Catholic University of Leuven (KUL)Herestraat 49 3000 Leuven Belgium Australian Key Centre for Microscopy and Microanalysis Electron Microscopy Unit The University of SydneySydney 2006NSWAustralia

Nowadays, liver metastasis remains difficult to cure. When tumor cells escape and arrive in the liver sinusoids, they encounter the local defense mechanism specific to the liver. The sinusoidal cells have been widely described in physiologic conditions and in relation to metastasis during the past 30 years. This paper provides an “overview” of how these cells function in health and in diseases such as

关键词： Metastasis Liver Colon carcinoma Sinusoidalcells Kupffer cells Liver sinusoidal endothelial cells Hepatic NK cells

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Multifaceted nature of membrane microdomains in colorectal cancer

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World Journal of Gastroenterology 2011年第6期17卷 681-690页

作者： Kristina A Jahn filip Braet Australian Centre for Microscopy and Microanalysis The University of Sydney

Membrane microdomains or lipid rafts are known to be highly dynamic and to act as selective signal transduction mediators that facilitate interactions between the cell's external and internal *** rafts play an important mediating role in the biology of cancer:they have been found in almost all existing experimental cancer models,including colorectal cancer (CRC),and play key regulatory roles in cell migration,metastasis,cell survival and tumor *** paper explores the current state of knowledge in this field by highlighting some of the pioneering and recent lipid raft studies performed on different CRC cell lines and human tissue *** this literature review,it becomes clear that membrane microdomains appear to be implicated in all key intracellular signaling pathways for lipid metabolism,drug resistance,cell adhesion,cell death,cell proliferation and many other processes in *** signal transduction pathways seem to originate directly from those peculiar lipid islands,thereby orchestrating the colon cancer cells' state and *** confirmed by recent animal and preclinical studies in different CRC models,continuing to unravel the structure and function of lipid rafts-including their associated complex signaling pathways-will likely bring us one step closer to better monitoring and treating of colon cancer patients.

关键词： Actin Caveolae Cytoskeleton Combined imaging Detergent-resistant membranes Drug targeting Electron microscopy Lipid domains Membrane rafts Prognosis Staging Tomography Lipid-mediated therapy

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Image-based appearance acquisition of effect coatings

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Computational Visual Media 2019年第1期5卷 73-89页

作者： Jirí filip Radomír Vávra The Czech Academy of Sciences Institute of Information Theory and AutomationPod Vodárenskou vezı4185 08Praha 8Czech Republic

Paint manufacturers strive to introduce unique visual effects to coatings in order to visually communicate functional properties of products using value-added, customized design. However, these effects often feature complex, angularly dependent, spatiallyvarying behavior, thus representing a challenge in digital reproduction. In this paper we analyze several approaches to capturing spatially-varying appearances of effect coatings. We compare a baseline approach based on a bidirectional texture function(BTF) with four variants of half-difference parameterization. Through a psychophysical study, we determine minimal sampling along individual dimensions of this *** conclude that, compared to BTF, bivariate representations better preserve visual fidelity of effect coatings, better characterizing near-specular behavior and significantly the restricting number of images which must be captured.

关键词： effect coatings measurement bidirectional texture function(BTF) appearance psychophysical experiment

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Comparative analysis of experimental testing procedures for the elicitation of rescue actions in ants

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Current Zoology 2022年第2期68卷 159-168页

作者： filip TURZA Krzysztof MILER Institute of Environmental Sciences Faculty of BiologyJagiellonian UniversityGronostajowa 7Krakow 30-387Poland Institute of Systematics and Evolution of Animals Polish Academy of SciencesStawkowska 17Krakow 31-016Poland

Rescue behavior is observed when 1 individual provides help to another individual in *** reports of rescue behavior concern ants(Formicidae),in which workers rescue each other from various types of *** of these entrapment situations can be simulated in the laboratory using an entrapment bioassay,in which ants confront a single endangered nest mate entrapped on a sandy arena by means of an artificial ***,we compared numerous characteristics of rescue actions(contact between individuals,digging around the entrapped individual,pulling at its body parts,transport of the sand covering it,and biting the snare entrapping it)in Formica cinerea *** performed entrapment tests in the field and in the laboratory,with the latter under varying conditions in terms of the number of ants potentially engaged in rescue actions and the arena substrate(marked or unmarked by ants'pheromones).Rescue actions were more probable and pronounced in the field than in the laboratory,regardless of the type of ***,different test types in the laboratory yielded inconsistent results and showed noteworthy variability depending on the tested characteristic of *** results illustrate the specifics of ant rescue actions elicited in the natural setting,which is especially important considering the scarcity of field ***,our results underline the challenges related to the comparison of results from different types of entrapment tests reported in the available ***,our study shows how animal behavior differs in differing experimental setups used to answer the same questions.

关键词： ants animal behavior entrapment bioassay Formica cinerea rescue behavior

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Parametric amplification as a single-shot time-resolved off-harmonic probe for laser–matter interactions

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High Power Laser Science and Engineering 2023年第4期11卷 9-16页

作者： filip Grepl Maksym Tryus Timofej Chagovets Daniele Margarone ELI Beamlines Facility The Extreme Light Infrastructure ERICDolníBˇrežanyCzech Republic Faculty of Nuclear Sciences and Physical Engineering Czech Technical University in PraguePragueCzech Republic

An optical probing of laser–plasma interactions can provide time-resolved measurements of plasma density;however,single-shot and multi-frame probing capabilities generally rely on complex setups with limited *** have demonstrated a new method for temporal resolution of the rapid dynamics(∼170 fs)of plasma evolution within a single laser shot based on the generation of several consecutive probe pulses from a single beta barium borate-based optical parametric amplifier using a fraction of the driver pulse with the possibility to adjust the central wavelengths and delays of particular pulses by optical delay *** flexibility and scalability of the proposed experimental technique are presented and discussed.

关键词： off-harmonic optical probing plasma diagnostics ultrafast imaging

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The role of interleukin-6 in central nervous system demyelination

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Neural Regeneration Research 2016年第12期11卷 1922-1923页

作者： filip Petkovic Bernardo Castellano Department of Cell Biology Physiology and Immunology Institute of Neuroscience Universitat Autonoma de Barcelona Bellaterra Spain Department of Immunology Institute for Biological Research "Sinisa Stankovic" Belgrade Serbia

Demyelination of the central nervous system（CNS）is a hallmark of multiple sclerosis（MS）,chronic inflammatory and neurodegenerative *** demyelination favors neurodegeneration of denuded axons,which is a major cause... 详细信息

关键词： interleukin disability neuronal irreversible despite microglia cytokine astrocyte maturation understood

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