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An alternative to MINFLUX that enables nanometer resolution in a confocal microscope

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Light(Science & Applications) 2022年第9期11卷 2077-2085页

作者： LUCIANO A.MASULLO ALAN M.SZALAI LUCIA F.LOPEZ MAURICIO PILO-PAIS GUILLERMO P.ACUNA fernando d.stefani Centro de Investigaciones en Bionanociencias(CIBION) Consejo Nacionallde Investigaciones Cientificas y Técnicas(CONICET)Godoy Cruz 2390C1425FQD Ciudad Autónoma de Buenos AiresBuenos AiresArgentina departamento de Fisica Facultad de Ciencias Exactas y NaturalesUniversidad de Buenos AiresGuiraldes 2620C1428EHA Ciudad Autónoma de Buenos AiresBuenos AiresArgentina department of Physics University of FribourgChemin du Musee 3Fribourg CH-1700Switzerland

Localization of single fluorescent emitters is key for physicochemical and biophysical measurements at the nanoscale and beyond ensemble *** include single-molecule tracking and super-resolution imaging by single-molecule localization *** the numerous localization methods available,MINFLUX outstands for achieving a~10-fold improvement in resolution over wide-field camera-based approaches,reaching the molecular scale at moderate photon *** application of MINFLUX and related methods has been hindered by the technical complexity of the ***,we present RASTMIN,a single-molecule localization method based on raster scanning a light pattern comprising a minimum of *** delivers~1-2nm localization precision with usual fluorophores and is easily implementable on a standard confocal microscope with few *** demonstrate the performance of RASTMIN in localization of single molecules and super-resolution imaging of dNA origami structures.

关键词： resolution pattern enable

英汉交替传译中译语冗余的成因及应对策略——以Ian Newton教授《新冠疫情下的难民问题》模拟会议为例

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英汉交替传译中译语冗余的成因及应对策略——以Ian Newton教授《...

作者： stefani Viviana 上海外国语大学

学位级别：硕士

在交替传译中,译员应该清晰以及简明地传播信息,但是过度冗余是口译当中地一个常见的问题。冗余是语言中不可缺少的。实际上,在沟通的过程,信息会遭受噪音等因素的干扰,冗余在一定的程度上能够避免因此导致的信息失真。但是在交替传译中... 详细信息

在交替传译中,译员应该清晰以及简明地传播信息,但是过度冗余是口译当中地一个常见的问题。冗余是语言中不可缺少的。实际上,在沟通的过程,信息会遭受噪音等因素的干扰,冗余在一定的程度上能够避免因此导致的信息失真。但是在交替传译中,过度冗余会降低信息传达的效率。冗余尤其会造成译语时间的延误、降低口译的流畅、使译语逻辑信息不明,同时导致听众需耗费大量精力去理解译语。本论文拟探讨的案例为探讨Ian Newton教授《新冠疫情下的难民问题》模拟会议。实际上,在本次模拟会议的英汉交替传译的过程中,译语出现多处冗余现象。笔者希望通过研究交替传译中的冗余现象以及其成因对此提出应对策略,以提升口译质量和效率。

关键词：冗余英汉交传自我修改

does needle calibre affect pain and complication rates in patients undergoing transperineal prostate biopsy？ A prospective, randomized trial

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Asian Journal of Andrology 2009年第6期11卷 678-682页

作者： Saredi Giovanni Maria Chiara Sighinolfi Fidanza Francesco de stefani Stefano Micali Salvatore Maurizio Paterlini Roberto d＇Amico Bianchi Giampaolo department of Urology University of Modena and Reggio Emilia Modena 41100 Italy department of Oncology University of Modena and Reggio Emilia Modena 41100 Italy

Transperineal prostate biopsy is a procedure that can be used to obtain histological samples from the prostate. To improve both the quality of the biopsy core samples and prostate cancer detection, we are currently performing a prospective, randomized trial comparing prostate biopsy samples obtained using an 18 G-needle to those obtained using a 16 G-needle. The aim of this preliminary study was to evaluate pain and complication rates in both groups in order to assess whether performing a prostate biopsy with a larger calibre needle is a feasible procedure. One hundred and eighty-seven patients undergoing transperineal prostate biopsy were prospectively evaluated and divided into two groups. The first group （94 patients, Group A） received a transperineal prostate biopsy using a 16 G-needle and the second group （93 patients, Group B） underwent transperineal prostate biopsy with an 18 G-needle. Anaesthesia was obtained with a single perineal injection at the prostatic apex in all subjects. A visual analogue scale （VAS） and facial expression scale （FES） were used to assess pain during multiple steps of the procedure in each group. A detailed questionnaire was used to obtain information about drug use because it could potentially influence the pain and complications that patients experienced. Two weeks after the procedure, early and late complications were evaluated. Statistical analysis was carried out using non-parametric tests. Prostate Specific Antigen （PSA） and drug use were similar at baseline between the two groups. Pain during prostate biopsy, which was measured with both the VAS and FES instruments, did not differ significantly between the 18- and 16 G-needle groups, and no significant differences were found in early or late complication rates between the groups. Transperineal prostate biopsy with a 16 G-needle is a feasible Further studies with larger patient populations are required to prostate cancer detection rates. procedure in terms of pain and complicati

关键词： needle calibre prostatic biopsy side effects

Molecular detection of vector-borne bacteria and protozoa in healthy hunting dogs from Central Italy

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Asian Pacific Journal of Tropical Biomedicine 2015年第2期5卷 108-112页

作者： Valentina Virginia Ebani Simona Nardoni Giulia Fognani Linda Mugnaini Fabrizio Bertelloni Guido Rocchigiani Roberto Amerigo Papini Francesco stefani Francesca Mancianti department of Veterinary Science University of Pisa Veterinary Clinic "Costa d'Argento" Orbetello(Grosseto)Italy

Objective:To determine the pi'evalence of vector-bome bacteria and protozoa in hunting dogs living in Central ***:Molecular testing was executed on dNA which was extracted from blood specimens collected from 117 asymptomatic dogs to detect Anaplasma phagocytophilum,Babesia canis(***),Bartonella spp..Coxiella burnetii(***).Ehrlichia *** *** Leislnnania ***:A total of 48 dogs(41.0%) were infested by Ixodes ricinus and Rhipicephalus sanguineus ***-borne infections were observed in 64(54.7%) *** in detail.38 dogs(32.5%) screened positive for Hepatozoon canis,24(20.5%) for Bartonella rinsonii ***.20(17.1%) for Leishmania infantum,6(5.1%) for ***,5(4.3%) for ***(3 *** vogeli and 2 *** canis),3(2.5%) for Anaplasma phagocytophilum,and 2(1.7%) for Ehrlichia *** infection by 2 agents occurred in 17(14.5%) subjects,by 3 agents in 7(6.0%) dogs,and by 4 agents in 1(0.9%) ***:The results demonstrated that several vector-borne pathogens were circulating in this region and dogs infected by these agents were usually asymptomatic.A relevant finding was the presence of dNA of ***,a severe zoonotic agent,in the 5.1% of tested dogs,which can be source of infection for their owners not only through tick bites,but also directly with urine,feces and birth products.

关键词： Arthropod-bornc infection Bacteria dog PCR Protozoa

Combining Contactless Inductive Flow Tomography and Mutual Inductance Tomography for Two-Phase Flow Measurements at a Continuous Casting Model

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Journal of Iron and Steel Research International 2012年第S1期19卷 479-482页

作者： stefani Frank ECKERT Sven GERBETH Gunter KLOTSCHE Konrad TIMMEL Klaus WONdRAK Thomas PEYTON Anthony J. TERZIJA Natasa YIN Wuliang Helmholtz-Zentrum dresden-Rossendorf P.O.Box 510119Dresden D-01314Germany School of Electrical and Electronic Engineering The University of ManchesterP.O.Box 88Sackville Street Manchester M13 9PLUK

The flow structure in the mould of a continuous caster is of key importance for the quality of the final product. The use of most conventional flow measurement techniques is prevented by the high temperature of the liquid *** a downscaled physical model of the continuous casting process,we present combined measurements of the flow in the mould by Contacfless Inductive Flow Tomography(CIFT),and of the conductivity distribution in the submerged entry nozzle by Mutual Inductance Tomograpliy(MIT).In addition,we summarize an experiment with a magnetic stirrer around the submerged entry nozzle and its effects on the flow in the *** new developments towards a robust implementation of CIFT at a real caster,including the use of pickup coils and gradiometric probes,are also discussed.

关键词： continuous casting flow measurements tomography

里氏结节——Ⅰ型神经纤维瘤病的标志及神经外胚叶分化的免疫组织化学证据

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德国医学 1999年第2期16卷 97-98页

作者： A.Lukacs A.K.Junk F.H.stefani A.Kampik C.G.Schirren G.Plewig 胡真保 dermatologische Klinik und Poliklinik Ludwig-Maximilians-UniversittFrauenlobstr.9-11D-80337 MünchenGermany 同济医科大学附属同济医院

神经纤维瘤病是常见的遗传病,分8个亚型,最常见的是Ⅰ型和Ⅱ型,其基因定位于17号和22号染色体。Ⅰ型的特征是有虹膜结节,1937年由Lisch作出诊断,里氏结节通常为多个分布在虹膜上,有些在裂隙灯下才能看见,常聚集在虹膜收缩轮和虹膜缘,边... 详细信息

神经纤维瘤病是常见的遗传病,分8个亚型,最常见的是Ⅰ型和Ⅱ型,其基因定位于17号和22号染色体。Ⅰ型的特征是有虹膜结节,1937年由Lisch作出诊断,里氏结节通常为多个分布在虹膜上,有些在裂隙灯下才能看见,常聚集在虹膜收缩轮和虹膜缘,边界清晰,呈圆形或卵圆形,部分呈多环状融合,凸起的结节位于虹膜前面,用斜照法清楚地看到从虹膜水平凸出的结节阴影,结节越大。

关键词：神经纤维瘤病里氏结节神经外胚叶

Stem/progenitor cells in fetuses and newborns:overview of immunohistochemical markers

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Cell Regeneration 2021年第1期10卷 239-250页

作者： d.Fanni C.Gerosa C.Loddo M.Castagnola V.Fanos M.Zaffanello G.Faa division of Pathology University Hospital San Giovanni Di Diovia Ospedale54CagliariItaly department of Biology College of Science and TechnologyTemple UniversityPhidelphiaUSA Neonatal Intensive Care Unit Department of Surgical SciencesUniversity of CagliariCagliariItaly Laboratory of Biochemistry and Metabolomics IRCCS Fondazione Santa LuciaRomeItaly department of Surgical Sciences DentistryGynecology and PediatricsUniversity of VeronaPiazzale Stefani1I-37126 VeronaItaly

Microanatomy of the vast majority of human organs at birth is characterized by marked differences as compared toadult organs, regarding their architecture and the cell types detectable at histology. In preterm neonates, thesedifferences are even more evident, due to the lower level of organ maturation and to ongoing cell *** of the most remarkable finding in preterm tissues is the presence of huge amounts of stem/progenitor cells inmultiple organs, including kidney, brain, heart, adrenals, and lungs. In other organs, such as liver, the completelydifferent burden of cell types in preterm infants is mainly related to the different function of the liver duringgestation, mainly focused on hematopoiesis, a function that is taken by bone marrow after birth. Our preliminarystudies showed that the antigens expressed by stem/progenitors differ significantly from one organ to the ***, within each developing human tissue, reactivity for different stem cell markers also changes duringgestation, according with the multiple differentiation steps encountered by each progenitor during development. Abetter knowledge of stem/progenitor cells of preterms will allow neonatologists to boost preterm organmaturation, favoring the differentiation of the multiple cells types that characterize each organ in at term neonates.

关键词： Fetus Immunohistochemical analysis Newborn Progenitor cells Stem cells