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How mesenchymal stem cell cotransplantation with hematopoietic stem cells can improve engraftment in animal models

World Journal of Stem Cells 2022年第8期14卷 658-679页

作者： Murilo Montenegro Garrigós fernando anselmo de oliveira Mariana Penteado Nucci Leopoldo Penteado Nucci Arielly da Hora Alves Olivia Furiama Metropolo Dias Lionel Fernel Gamarra Hospital Israelita Albert Einstein São Paulo 05652-900São PauloBrazil Instituto de Química Universidade de São PauloSão Paulo 05508-900São PauloBrazil LIM44-Hospital das Clínicas Faculdade Medicina da Universidade de São PauloSão Paulo 05403-000Brazil Centro Universitário do Planalto Central Área Especial para Industria nº02 Setor Leste-Gama-DFBrasília 72445-020Distrito FederalBrazil

BACKGROUND Bone marrow transplantation(BMT)can be applied to both hematopoietic and nonhematopoietic diseases;nonetheless,it still comes with a number of challenges and limitations that contribute to treatment *** this in mind,a possible way to increase the success rate of BMT would be cotransplantation of mesenchymal stem cells(MSCs)and hematopoietic stem cells(HSCs)to improve the bone marrow niche and secrete molecules that enhance the hematopoietic *** To analyze HSC and MSC characteristics and their interactions through cotransplantation in murine *** We searched for original articles indexed in PubMed and Scopus during the last decade that used HSC and MSC cotransplantation and in vivo BMT in animal models while evaluating cell *** excluded in vitro studies or studies that involved graft versus host disease or other hematological diseases and publications in languages other than *** PubMed,we initially identified 555 articles and after selection,only 12 were *** Scopus,2010 were identified,and six were left after the screening and eligibility *** Of the 2565 articles found in the databases,only 18 original studies met the eligibility *** distribution by source showed similar ratios,with human umbilical cord blood or animal bone marrow being administered mainly with a dose of 1×10^(7) cells by intravenous or intrabone ***,MSCs had a high prevalence of human donors with a variety of sources(umbilical cord blood,bone marrow,tonsil,adipose tissue or fetal lung),using a lower dose,mainly 106 cells and ranging 104 to 1.5×107 cells,utilizing the same *** were characterized prior to administration in almost every *** recipient used was mostly immunodeficient mice submitted to low-dose irradiation or *** main technique of engraftment for HSC and MSC cotransplantation evaluation was chimerism,followed by hematopoietic reconstitution and survival anal

关键词： Mesenchymal stem cells Hematopoietic stem cells Bone marrow transplantation Cotransplantation Hematopoietic reconstitution Engraftment

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Stem cell homing, tracking and therapeutic efficiency evaluation for stroke treatment using nanoparticles: A systematic review

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World Journal of Stem Cells 2020年第5期12卷 381-405页

作者： Mariana Penteado Nucci Igor Salerno Filgueiras Joao Matias Ferreira fernando anselmo de oliveira Leopoldo Penteado Nucci Javier Bustamante Mamani Gabriel Nery Albuquerque Rego Lionel Fernel Gamarra LIM44 Hospital das Clinicas HCFMUSPFaculdade de MedicinaUniversidade de Sao PauloSao Paulo 05529-060Brazil Hospital Israelita Albert Einstein Sao Paulo 05529-060Brazil Centro Universitário do Planalto Central BrasíliaDFBrazil

BACKGROUND Stroke is the second leading cause of death *** is a real need to develop treatment strategies for reducing neurological deficits in stroke survivors,and stem cell(SC)therapeutics appear to be a promising alternative for stroke therapy that can be used in combination with approved thrombolytic or thrombectomy ***,the efficacy of SC therapy depends on the SC homing ability and engraftment into the injury site over a long period of ***,tracking SCs from their niche to the target tissues is a complex *** To evaluate SC migration homing,tracking and therapeutic efficacy in the treatment of stroke using nanoparticles METHODS A systematic literature search was performed to identify articles published prior to November 2019 that were indexed in PubMed and *** following inclusion criteria were used:(1)Studies that used in vivo models of stroke or ischemic brain lesions;(2)Studies of SCs labeled with some type of contrast agent for cell migration detection;and(3)Studies that involved in vivo cellular homing and tracking *** A total of 82 articles were identified by indexing in Scopus and Pub *** inclusion criteria were applied,35 studies were selected,and the articles were assessed for eligibility;ultimately,only 25 studies were *** of the selected studies used SCs from human and mouse bone marrow labeled with magnetic nanoparticles alone or combined with fluorophore *** cells were administered in the stroke model(to treat middle cerebral artery occlusion in 74%of studies and for photothrombotic induction in 26%of studies).Fiftythree percent of studies used xenogeneic grafts for cell therapy,and the migration homing and tracking evaluation was performed by magnetic resonance imaging as well as other techniques,such as near-infrared fluorescence imaging(12%)or bioluminescence assays(12%).CONCLUSION Our systematic review provided an up-to-date evaluation of SC migration hom

关键词： Stem cell Nanoparticles Homing Tracking Near-infrared fluorescence image Cellular therapy Magnetic resonance image Bioluminescence Stroke

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Current overview of induced pluripotent stem cell-based blood-brain barrier-on-a-chip

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World Journal of Stem Cells 2023年第6期15卷 632-651页

作者： Arielly da Hora Alves Mariana Penteado Nucci Nicole Mastandrea Ennes do Valle Juliana Morais Missina Javier Bustamante Mamani Gabriel Nery Albuquerque Rego Olivia Furiama Metropolo Dias Murilo Montenegro Garrigós fernando anselmo de oliveira Lionel Fernel Gamarra Hospital Israelita Albert Einstein São Paulo 05529-060São PauloBrazil Hospital das Clínicas HCFMUSP Faculdade de MedicinaUniversidade de São PauloSão Paulo 05403-010São PauloBrazil

BACKGROUND Induced pluripotent stem cells(iPSCs)show great ability to differentiate into any tissue,making them attractive candidates for pathophysiological *** rise of organ-on-a-chip technology in the past century has introduced a novel way to make in vitro cell cultures that more closely resemble their in vivo environments,both structural and *** literature still lacks consensus on the best conditions to mimic the blood-brain barrier(BBB)for drug screening and other personalized *** development of models based on BBB-on-achip using iPSCs is promising and is a potential alternative to the use of animals in *** To analyze the literature for BBB models on-a-chip involving iPSCs,describe the microdevices,the BBB in vitro construction,and *** We searched for original articles indexed in PubMed and Scopus that used iPSCs to mimic the BBB and its microenvironment in microfluidic *** articles were identified,wherein only 14 articles were finally selected according to the inclusion and exclusion *** compiled from the selected articles were organized into four topics:(1)Microfluidic devices design and fabrication;(2)characteristics of the iPSCs used in the BBB model and their differentiation conditions;(3)BBB-on-a-chip reconstruction process;and(4)applications of BBB microfluidic three-dimensional models using *** This study showed that BBB models with iPSCs in microdevices are quite novel in scientific *** technological advances in this area regarding the use of commercial BBB-on-a-chip were identified in the most recent articles by different research *** polydimethylsiloxane was the most used material to fabricate in-house chips(57%),whereas few studies(14.3%)adopted *** the models were constructed using a porous membrane made of diverse materials to separate the *** sources were divergent among the studies,but th

关键词： Induced pluripotent stem cells Cell differentiation Blood-brain barrier Neurovascular unit Organ-on-a-chip Microfluidic device

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Triple-modal imaging of stem-cells labeled with multimodal nanoparticles, applied in a stroke model

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World Journal of Stem Cells 2019年第2期11卷 100-123页

作者： Helio Rodrigues da Silva Javier Bustamante Mamani Mariana Penteado Nucci Leopoldo Penteado Nucci Andrea Tiemi Kondo Daianne Maciely Carvalho Fantacini Lucas Eduardo Botelho de Souza Virginia Picanço-Castro Dimas Tadeu Covas José Mauro Kutner fernando anselmo de oliveira Nelson Hamerschlak Lionel Fernel Gamarra Hospital Israelita Albert Einstein LIM44 Hospital das Clinicas HCFMUSP Faculdade de MedicinaUniversidade de São Paulo Centro Universitário do Planalto Central Faculdade de Medicina de Ribeirão Preto Universidade de São Paulo

BACKGROUND Mesenchymal stem cells(MSCs) have been widely tested for their therapeutic efficacy in the ischemic brain and have been shown to provide several benefits. A major obstacle to the clinical translation of these therapies has been the inability to noninvasively monitor the best route, cell doses, and collateral effects while ensuring the survival and effective biological functioning of the transplanted stem cells. Technological advances in multimodal imaging have allowed in vivo monitoring of the biodistribution and viability of transplanted stem cells due to a combination of imaging technologies associated with multimodal nanoparticles(MNPs) using new labels and covers to achieve low toxicity and longtime residence in *** To evaluate the sensitivity of triple-modal imaging of stem cells labeled with MNPs and applied in a stroke *** After the isolation and immunophenotypic characterization of human bonemarrow MSCs(hBM-MSCs), our team carried out lentiviral transduction of these cells for the evaluation of bioluminescent images(BLIs) in vitro and in vivo. In addition, MNPs that were previously characterized(regarding hydrodynamic size, zeta potential, and optical properties), and were used to label these cells,analyze cell viability via the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay and BLI analysis, and quantify the internalization process and iron load in different concentrations of MNPs via magnetic resonance imaging(MRI),near-infrared fluorescence(NIRF), and inductively coupled plasma-mass spectrometry(ICP-MS). In in vivo analyses, the same labeled cells were implanted in a sham group and a stroke group at different times and under different MNP concentrations(after 4 h or 6 d of cell implantation) to evaluate the sensitivity of triple-modal *** hBM-MSC collection and isolation after immunophenotypic characterization were demonstrated to be adequate in hBM samples. After transduction of these cells with

关键词： Multimodal nanoparticles Human bone marrow mesenchymal stem cells Near-infrared fluorescence image Magnetic resonance image Bioluminescence Stroke

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Effect of resistance training volume on body adiposity,metabolic risk,and inflammation in postmenopausal and older females:Systematic review and meta-analysis of randomized controlled trials

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Journal of Sport and Health Science 2024年第2期13卷 145-159页

作者： Paulo Ricardo Prado Nunes Pamela Castro-e-Souza anselmo Alves de oliveira Bruno de Freitas Camilo Gislaine Cristina-Souza Lucio Marques Vieira-Souza Marcelo Augusto da Silva Carneiro department of Body and Human Movement Minas Gerais State University(UEMG)37902-092 PassosMGBrazil University Center of Planalto de Araxa(UNIARAXA) 38180-129 AraxaMGBrazil Physical Activity Health and Ageing Research Group(GPASE)Minas Gerais State University(UEMG)37902-092 PassosMGBrazil Exercise and Nutrition Research Group(GPEN) Minas Gerais State University(UEMG)37902-092 PassosMGBrazil Sport Sciences and Physical Exercise Study and Research Group(GEPCEEX) Minas Gerais State University(UEMG)37902-092 PassosMGBrazil Metabolism Nutrition and Exercise LaboratoryPhysical Education and Sport CenterLondrina State University(UEL)86050-070 LondrinaPRBrazil Postgraduate Program in Physical Education Federal University of Sergipe(UFS)49107-230 Sao CristovaoSEBrazil

Purpose:This meta-analytical study aimed to explore the effects of resistance training(RT) volume on body adiposity,metabolic risk,and inflammation in postmenopausal and older ***:A systematic search was performed for randomized controlled trials in PubMed,Scopus,Web of Science,and *** controlled trials with postmenopausal and older females that compared RT effects on body adiposity,metabolic risk,and inflammation with a control group(CG) were *** reviewers selected the studies,extracted the data,and performed the risk of bias and certainty of the evidence(Grading of Recommendations,Assessment,development,and Evaluation(GRAde)) *** body and abdominal adiposity,blood lipids,glucose,and C-reactive protein were included for meta-analysis.A random-effects model,standardized mean difference(Hedges’ g),and 95% confidence interval(95%CI) were used for ***:Twenty randomized controlled trials(overall risk of bias:some concerns;GRAde:low to very low) with overweight/obese postmenopausal and older females were *** groups were divided into low-volume RT(LVRT,~44 sets/week) and high-volume RT(HVRT,~77 sets/week).Both RT groups presented improved body adiposity,metabolic risk,and inflammation when compared to ***,HVRT demonstrated higher effect sizes than LVRT for glucose(HVRT=-1.19;95%CI:-1.63 to-0.74;LVRT=-0.78;95%CI:-1.15 to-0.41) and C-reactive protein(HVRT=-1.00;95%CI:-1.32 to-0.67;LVRT=-0.34;95%CI,-0.63 to-0.04)) when compared to ***:Compared to CG,HVRT protocols elicit greater improvements in metabolic risk and inflammation outcomes than LVRT in overweight/obese postmenopausal and older females.

关键词： C-reactive protein Fat mass Lipid profile Menopause Strength training

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Anti-inflammatory effects ofα-humulene andβ-caryophyllene on pterygium fibroblasts

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International Journal of Ophthalmology(English edition) 2022年第12期15卷 1903-1907页

作者： Magda Massae Hata Viveiros Márcia Guimarães Silva JoséGalberto Martins da Costa anselmo Gomes de oliveira Carolina Rubio Carlos Roberto Padovani Cláudia Aparecida Rainho Silvana Artioli Schellini department of Ophthalmology School of Medicine of BotucatuSão Paulo State University–UNESPBotucatuSao Paulo 18618-000Brazil department of Pathology School of Medicine of BotucatuSão Paulo State University–UNESPBotucatuSao Paulo 01049-010Brazil department of Biological Chemistry Regional University of CaririCratoCeará66100-900Brazil department of Pharmacology School of Pharmaceutical SciencesSão Paulo State University–UNESPAraraquaraSao Paulo 01049-010Brazil Biological Sciences Institute of Biosciences of BotucatuSão Paulo State University–UNESPBotucatuSao Paulo 01049-010Brazil department of Biostatistics São Paulo State University–UNESPBotucatuSao Paulo 01049-010Brazil

AIM:To investigate the anti-inflammatory effects of the sesquiterpenesα-humulene andβ-caryophyllene on pterygium ***:Primary cultures of pterygium fibroblasts were *** passage pterygium fibroblasts were exposed toα-humulene andβ-caryophyllene separately and *** cell viability was assessed by 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide(MTT)assay at 12,24,48,and 72h after *** levels of the inflammatory cytokines interleukin(IL)-1β,IL-6,IL-8,tumor necrosis factor(TNF)-αand IL-10 in the conditioned culture medium were assessed by enzymelinked immunosorbent assay(ELISA)at 12,24 and 48h after *** were statistically analyzed using Friedman repeated measures analysis of variances on ***:The 25μmol/Lβ-caryophyllene induced significant decrease in the IL-6 production by pterygium fibroblasts 48h after the exposure(P=0.041).The levels of IL-1β,IL-8,IL-10,and TNF-αwere very low and had no statistically significant variations af ter exposure toα-humulene,β-caryophyllene,or both compounds ***:The exposure to 25μmol/L ofβ-caryophyllene significantly reduce the production of IL-6 by pterygium fibroblasts after *** sesquiterpene may be a potential alternative adjuvant agent for the treatment of pterygium.

关键词： pterygium fibroblasts β-caryophyllene α-humulene cytokines

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Study and Evaluation of Aluminum Capsules to Irradiation of Gaseous Samples in Nuclear Reactor

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Journal of Physical Science and Application 2015年第4期5卷 263-267页

作者： Osvaldo Luiz da Costa anselmo Feher Joao A. Moura Carla D. Souza Rodrigo Tiezzi Daiane C. B. de Souza Eduardo S. Moura Henrique B. oliveira Carlos A. Zeituni Maria Elisa C. M. Rostelato Nuclear and Energy Research Institute IPEN Av. Professor Lineu Prestes 2242 Cidade Universitazria Sao Paulo SP 05508-000 Brazil

Gas irradiation in research nuclear reactors is an important way to produce radionuclides. Although some nuclear reactors centers offer this type of service, there are few publications about capsules to irradiation of gaseous samples. This paper describes a method to fabricate and evaluate aluminum capsules to irradiate gaseous samples in nuclear reactor. A semi-circular slotted die from a hydraulic presshead was modified to seal aluminum tubes. The aluminum capsules were subjected to leak detection tests, which demonstrated the accordance with standard ISO 9978.

关键词： Aluminum capsules gas irradiation ISO 9978 research nuclear reactor tightness.

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